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A major quantitative trait locus controlling phosphorus utilization efficiency under different phytate-P conditions at vegetative stage in barley

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Journal of Integrative Agriculture 2018年第2期17卷 285-295页

作者： GAO Shang-qing CHEN Guang-deng HU De-yi ZHANG xi-zhou LI Ting-xuan LIU Shi-hang LIU Chun-ji College of Resources Sichuan Agricultural University Chengdu 611130 P.R. China Triticeae Research Institute Sichuan Agricultural University Chengdu 611130 P.R. China Commonwealth Scientific and Industrial Research Organization (CSIRO) Agriculture Flagship St Lucia Queensland 4067 Australia

Organic phosphorus（P） is an important component of the soil P pool, and it has been proven to be a potential source of P for plants. The phosphorus utilization efficiency（PUE） and PUE related traits（tiller number（TN）, shoot dry weight（DW）, and root dry weight） under different phytate-P conditions（low phytate-P, 0.05 mmol L^-1 and normal phytate-P, 0.5 mmol L^-1） were investigated using a population consisting of 128 recombinant inbred lines（RILs） at the vegetative stage in barley. The population was derived from a cross between a P-inefficient genotype（Baudin） and a P-efficient genotype（CN4027, a Hordeum spontaneum accession）. A major locus（designated ***-3 H） conferring PUE was detected in shoots and roots from the RIL population. The quantitative trait locus（QTL） was mapped on chromosome 3 H and the allele from CN4027 confers high PUE. This locus explained up to 30.3 and 28.4% of the phenotypic variance in shoots under low and normal phytate-P conditions, respectively. It also explains 28.3 and 30.7% of the phenotypic variation in root under the low and normal phytate-P conditions, respectively. Results from this study also showed that TN was not correlated with PUE, and a QTL controlling TN was detected on chromosome 5 H. However, dry weight（DW） was significantly and positively correlated with PUE, and a QTL controlling DW was detected near the ***-3 H locus. Based on a covariance analysis, existing data indicated that, although DW may affect PUE, different genes at this locus are likely involved in controlling these two traits.

关键词： barley phosphorus utilization efficiency quantitative trait locus recombinant inbred line phytate-P

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Synthesis of p-nitrophenol under normal pressure with A-1 phase transfer catalysis

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Journal of Central South University of Technology 2001年第1期8卷 54-56页

作者： ZHONG Shi an, OUYANG xi, zhou Chun shan, CHENG Ben cheng (College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China) 1. College of Chemistry and Chemical Engineering Central South University 410083 Changsha China

p nitrophenol is synthesized using p nitrochlorobenzene and sodium hydroxide with phase transfer catalyst. Several reaction factors, such as catalyst type, the amounts of catalysts and sodium hydroxide, the concentration of sodium hydroxide solution as well as reaction time, affect greatly the yield of p nitrophenol. The optimum reaction conditions are as follows: A 1 used as phase transfer catalyst whose consumption is 6% (mole fraction) of p nitrochlorobenzene; the molar ratio of sodium hydroxide to p nitrochlorobenzene is 3:1; the concentration of sodium hydroxide solution is 50% (mass fraction). Nitrobenzene, whose molar amount is 2.45 times as much as that of p nitrochlorobenzene; reaction time is 7 h at 140 ℃ under normal pressure. The final yield of p nitrophenol is 83.6%.

关键词： phase transfer catalysis p nitrophenol synthesis

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Preliminary report on screening IGSF3 gene mutation in families with congenital absence of lacrimal puncta and canaliculi

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International Journal of Ophthalmology(English edition) 2020年第9期13卷 1351-1355页

作者： Fei Wang Hai Tao Cui Han Fang Bai Peng Wang xi-Bin zhou Li-Hua Wang Chuan Liu Lacrimal Center of Ophthalmology the Third Medical Center of Chinese PLA General HospitalBeijing 100039China

AIM:To investigate the variation of IGSF3 gene in three families with congenital absence of lacrimal puncta and canaliculi,and to lay a foundation for further research on the pathogenic gene of congenital lacrimal duct ***:The members of the three families were *** ophthalmologic examinations in details,including slit-lamp biomicroscope,intraocular pressure and fundus examination,*** carried *** patients were checked with paracentesis of puncta membrane and lacrimal duct probing,as well as the computed tomographydacryocystography(CT-DCG).Peripheral blood of 14 participants(3 normal)from three families were collected,4 mL each,for genomic DNA extraction,and 11 exon fragments of IGSF3 gene were amplified and sequenced by polymerase chain reaction(PCR)to determine whether there were IGSF3 genetic ***:A total of 14 members from three families were screened for 4 synonymous variants:c.930 C>T(***366=),c.1359 T>C(***709=),c.1797 G>A(***855=),c.1539 G>A(***769=),and 6 missense variants:c.l507 G>A(***759 Ser),c.1783 T>C(***851 Arg),c.1952 G>T(*** 907 lle),c.3120 C>G(***1040 Glu),c.3123 C>G(***1041 Glu),c.3139_3140 insGAC(***1046_Pro1047 insAsp),and the latter three were only found in two patients with absence of lacrimal puncta and canaliculi combined with congenital osseous nasolacrimal canal obstruction from the first ***:The same IGSF3 gene mutation c.31393140 insGAC is found in the patients with congenital absence of lacrimal puncta and canaliculi combine with osseous nasolacrimal canal obstruction.

关键词： congenital absence lacrimal puncta lacrimal canaliculi IGSF3 mutation DNA sequencing

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SARS-Coronavirus-2 Nsp13 Possesses NTPase and RNA Helicase Activities That Can Be Inhibited by Bismuth Salts

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Virologica Sinica 2020年第3期35卷 321-329页

作者： Ting Shu Muhan Huang Di Wu Yujie Ren Xueyi Zhang Yang Han Jingfang Mu Ruibing Wang Yang Qiu Ding-Yu Zhang xi zhou Center for Translational Medicine Wuhan Jinyintan HospitalWuhan 430023China State Key Laboratory of Virology Wuhan Institute of VirologyCenter for Biosafety Mega-ScienceChinese Academy of SciencesWuhan 430071China Center for Precision Translational Medicine of Wuhan Institute of Virology and Guangzhou Women and Children’s Medical Center Guangzhou Women and Children’s Medical CenterGuangzhou 510120China State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical SciencesUniversity of MacaoMacao SAR 999078China University of Chinese Academy of Sciences Beijing 100049China

The ongoing outbreak of Coronavirus Disease 2019(COVID-19)has become a global public health ***-2(SARS-CoV-2),the causative pathogen of COVID-19,is a positive-sense single-stranded RNA virus belonging to the family *** RNA viruses,virus-encoded RNA helicases have long been recognized to play pivotal roles during viral life cycles by facilitating the correct folding and replication of viral ***,our studies show that SARS-CoV-2-encoded nonstructural protein 13(nsp13)possesses the nucleoside triphosphate hydrolase(NTPase)and RNA helicase activities that can hydrolyze all types of NTPs and unwind RNA helices dependently of the presence of NTP,and further characterize the biochemical characteristics of these two enzymatic activities associated with SARS-CoV-2 ***,we found that some bismuth salts could effectively inhibit both the NTPase and RNA helicase activities of SARS-CoV-2 nsp13 in a dose-dependent ***,our findings demonstrate the NTPase and helicase activities of SARS-CoV-2 nsp13,which may play an important role in SARS-CoV-2 replication and serve as a target for antivirals.

关键词： SARS-coronavirus-2(SARS-CoV-2) Nsp13 NTPase Helicase Antiviral target

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Newly discovered insect RNA viruses in China

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Science China(Life Sciences) 2013年第8期56卷 711-714页

作者： QIU Yang WANG ZhaoWei LIU Yongxiang QI Nan SI Jie xiANG Xue xiA xiaoLing HU YuanYang zhou xi State Key Laboratory of Virology College of Life SciencesWuhan University

Insects are a group of arthropods and the largest group of animals on Earth,with over one million species described to *** other life forms,insects suffer from viruses that cause disease and *** that are pathogenic to beneficial insects cause dramatic economic losses on *** contrast,viruses that are pathogenic to insect pests can be exploited as attractive biological control *** of these factors have led to an explosion in the amount of research into insect viruses in recent years,generating impressive quantities of information on the molecular and cellular biology of these *** to the wide variety of insect viruses,a better understanding of these viruses will expand our overall knowledge of their ***,we review studies of several newly discovered RNA insect viruses in China.

关键词： insect virus Wuhan Nodavirus Dendrolimus punctatus tetravirus Ectropis obliqua picorna-like virus

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Crushing analysis and multi-objective optimization of bitubular hexagonal columns with ribs

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Journal of Central South University 2017年第5期24卷 1164-1173页

作者： ZOU xiang GAO Guang-jun DONG Hai-peng LI Jian zhou xi-sai CHEN Wei GUAN Wei-yuan Key Laboratory of Traffic Safety on Track of Ministry of Education(School of Traffic&Transportation Engineering Central South University)Changsha 410075China

In order to improve the crashworthiness of thin-walled columns, the energy absorption characteristics of three columns under quasi-static axial crushing loads were analyzed through LS-DYNA. Numerical results show that the energy absorption capability of the bitubular hexagonal columns with middle to middle(MTM) ribs is the best, followed by the bitubular hexagonal columns with corner to corner(CTC) ribs and the bitubular hexagonal columns without(NOT) ribs, respectively. Then, the MTM rib was optimized by using multi-objective particle swarm optimization algorithm. Through the analysis of the Pareto front for specific energy absorption(SEA, A_(se)) and peak crushing force(PCF, F_(pc)), it is found that there is a vertex on the Pareto front. The vertex has the design parameters of t_1=1.2 mm, t_2=1.2 mm, A_(se)=11.3729 k J/kg, F_(pc)=235.8491 kN. When the PCF is in a certain size, on the left of the vertex, the point with t_2=1.2 mm has the biggest SEA, meanwhile on the right of the vertex, the point with t_1=1.2 mm has the biggest SEA. Finally, the global sensitivity analysis was conducted to investigate the effect of two design parameters. The result is obtained that both SEA and PCF for MTM are more sensitive to t_1 rather than t_2 in the design domain.

关键词： bitubular hexagonal column axial crushing crashworthiness optimization global sensitivity analysis

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G^2 Continuity Algorithms between Adjacent NURBS Patches along Common Cubic Boundary Curve

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Chinese Journal of Aeronautics 2003年第4期16卷 241-246,242-246页

作者：周西军 Mechanical and Electrical Engineering College Northwestern Polytechnical University Xi'an 710072 China

Non-uniform rational B-spline (NURBS) curves and surfaces are becoming increasingly widespread. The author have explored G^1 continuity condition between adjacent NURBS surface patches along common cubic boundary curve. On the basis of the research performed, this paper presents a G^2 continuity condition between adjacent NURBS patches along common cubic boundary curve and deduces a specific algorithm for contro1 points and weights of NURBS patch. For making another NURBS patch and one given NURBS patch to attain G^2, according to algorithm condition, one can adjust another patch control points and weights. It is much more convenient for engineers to apply.

关键词： computer aided design geometric continuity non-uniform rational B-spline

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Exosomal miR-29b found in aqueous humour mediates calcium signaling in diabetic patients with cataract

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International Journal of Ophthalmology(English edition) 2021年第10期14卷 1484-1491页

作者： Chao Gao xin Liu Fan Fan Jia-Ning Yang xi-Yue zhou Heng-Jun Mei xiao-Lei Lin Yi Luo First Affiliated Hospital School of MedicineShihezi UniversityShihezi 832000Xinjiang Uygur Autonomous RegionChina Eye Institute Eye and ENT HospitalCollege of MedicineFudan UniversityShanghai 200031China State Key Laboratory of Medical Neurobiology Institutes of Brain Science and Collaborative Innovation Center for Brain ScienceShanghai Medical CollegeFudan UniversityShanghai 200031China Shanghai Key Laboratory of Visual Impairment and Restoration Science and Technology Commission of Shanghai MunicipalityShanghai 200031China Key Laboratory of Myopia(Fudan University) Chinese Academy of Medical SciencesNational Health CommissionShanghai 200031China

AIM:To investigate the role of exosomal miR-29b and Ca^(2+)in regulating the function of human lens epithelial cells(HLECs).METHODS:Exosomes were isolated from human aqueous humour(AH)by ultracentrifugation,and visualized by nanopar ticle tracking and transmission electron *** miRNA sequencing was performed to identify differentially expressed miRNAs between diabetes with cataracts(DMC)group and age-related cataracts(ARC)*** was used to predict potential target of certain *** expression of CACNA1C mRNA was determined by quantitative real-time polymerase chain reaction and CACNA1C protein was determined by Western *** of Ca^(2+)in human AH and the culture supernatant of cells were detected by the calcium assay *** counting kit-8 was used to determine cell ***:Exosomes were isolated from human AH,which had a typical cup-shaped phenotype and a particle size distribution in accordance with micro extracellular *** miRNA sequencing revealed that miR-29b was significantly downregulated in DMC group compared with ***^(2+)concentration of human AH in DMC was higher than that in *** culture supernatant of cells transfected with miR-29b inhibitors had a higher concentration of Ca^(2+)than that transfected with miR-29b ***-29b reduced the viability of HLECs by upregulating CACNA1C ***:Exosomes isolated from human AH contains abundant miRNAs.A significantly expressed miR NA,miR-29b,can affect the concentration of Ca^(2+)and regulate HLEC processes by upregulating CACNA1C.

关键词： exosomes miR-29b diabetes and cataracts Ca^(2+) CACNA1C

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Glial cell line-derived neurotrophic factor and brain-derived neurotrophic factor regulate the interaction between astrocytes and Schwann cells at the trigeminal root entry zone

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Neural Regeneration Research 2023年第6期18卷 1364-1370页

作者： Madeha Ishag Adam Ling Lin Amir Mahmoud Makin xiao-Fen Zhang Lu-xi zhou xin-Yue Liao Li Zhao Feng Wang Dao-Shu Luo Key Laboratory of Brain Aging and Neurodegenerative Diseases of Fujian Province Laboratory of Clinical Applied AnatomySchool of Basic Medical SciencesFujian Medical UniversityFuzhouFujian ProvinceChina Public Technology Service Center of Fujian Medical University FuzhouFujian ProvinceChina Center for Membrane and Water Science&Technology Institute of Oceanic and Environmental Chemical EngineeringZhejiang University of TechnologyHangzhouZhejiang ProvinceChina

The trigeminal root entry zone is the zone at which the myelination switches from peripheral Schwann cells to central *** special anatomical and physiological structure renders it susceptible to nerve *** etiology of most primary trigeminal neuralgia is closely related to microvascular compression of the trigeminal root entry *** study aimed to develop an efficient in vitro model mimicking the glial environment of trigeminal root entry zone as a tool to investigate the effects of glial cell line-derived neurotrophic factor and brain-derived neurotrophic factor on the structural and functional integrity of trigeminal root entry zone and modulation of cellular *** astrocytes and Schwann cells isolated from trigeminal root entry zone of postnatal rats were inoculated into a two-well silicon culture insert to mimic the trigeminal root entry zone microenvironment and treated with glial cell line-derived neurotrophic factor and brain-derived neurotrophic *** monoculture,glial cell line-derived neurotrophic factor promoted the migration of Schwann cells,but it did not have effects on the migration of *** the co-culture system,glial cell line-derived neurotrophic factor promoted the bidirectional migration of astrocytes and Schwann ***-derived neurotrophic factor markedly promoted the activation and migration of ***,in the co-culture system,brain-derived neurotrophic factor inhibited the migration of astrocytes and Schwann cells to a certain *** findings suggest that glial cell line-derived neurotrophic factor and brain-derived neurotrophic factor are involved in the regulation of the astrocyte-Schwann cell interaction in the co-culture system derived from the trigeminal root entry *** system can be used as a cell model to study the mechanism of glial dysregulation associated with trigeminal nerve injury and possible therapeutic interventions.

关键词： astrocytes brain-derived neurotrophic factor cell migration glial cell line-derived neurotrophic factor glial interaction Schwann cells trigeminal nerve

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Structural insight into EV-A713A protein and its interaction with a peptide inhibitor

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Virologica Sinica 2023年第6期38卷 975-979页

作者： Yahui Liu Panjing Lv Wei Wang Jiahai Zhang xi zhou Yang Qiu Kun Cai Haoran Zhang Yuan Fang Yan Li Department of Pathogen Biology School of Basic MedicineTongji Medical College and State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious DiseasesHuazhong University of Science and TechnologyWuhan430030China Medical Subcenter of HUST Analytical&Testing Center Huazhong University of Science and TechnologyWuhan430030China MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics School of Life SciencesDivision of Life Sciences and MedicineUniversity of Science and Technology of Chin1Hefei230027China State Key Laboratory of Virology Wuhan Institute of VirologyChinese Academy of SciencesWuhan430071China Institute of Health Inspection and Testing Hubei Provincial Center for Disease Control and Prevention(Hubei CDC)Wuhan430079China Department of Pediatrics Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China

Dear Editor,Hand,foot,and mouth disease(HFMD)is a common illness among children that is usually mild and ***,in some severe cases,patients may rapidly develop neurological and systemic complications that can be fatal(Ooi et al.,2010).HFMD is caused by a few serotypes of human enterovirus A(HEV-A)species(Yu and Cowling,2019),with enterovirus A71(EV-A71)and coxsackievirus A16(CV-A16)being the most typical causative agents(Yu and Cowling,2019).Furthermore,severe neurological sequelae are more common in cases with EV-A71 serotype infection than other serotypes(Ooi et al.,2010;Yu and Cowling,2019).There has been a significant increase in EV-A71 epidemic activity throughout the Asia–Pacific region(McMinn,2002)since EV-A71 was first isolated from a patient with central nervous system(CNS)disease in 1969(Schmidt et al.,1974).EV-A71 is a small non-enveloped virus belonging to HEV-A species of the genus Enterovirus in the family Picornaviridae(Nasri et al.,2007).Its genome is a single-stranded positive-sense RNA of approximately 7400 bp in length and contains only one complete open reading frame(ORF),which encodes a large polyprotein(Solomon et al.,2010).

关键词： enterovirus insight

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