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Rice ethylene signaling-emerging from underwater

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Rice ethylene signaling-emerging from underwater

Transgenic Rice Expressing Chitinase Specific dsRNAs Coferred Resistance to Mythimna separata

中国遗传学会植物遗传和基因组学专业委员会2009年学术研讨会

作者： hada wuriyanghan Plant Gene Research Center National Key Lab of Plant GenomicsInstitute of Genetics and Developmental BiologyChinese Academy of Sciences

Ethylene regulates multiple aspects of plant growth and development,however,its roles in monocotyledonous plants are less *** receptors are the first component of ethylene *** this study,we characterized a subfamily II ethylene receptor OsETR2 in ***2 receptor has autophosphorylation ability,is a Ser/Thr kinase but not a His

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Transgenic Rice Expressing Chitinase Specific dsRNAs Coferre...

Control of Mythimna Separata using RNAi technology

中国植物病理学会2016年学术年会

作者：包文化 hada wuriyanghan University of Inner Mongolia

Mythimna Separata is major agricultural pests that cause significant yield losses of crop plants each *** of double-stranded RNA（dsRNA） directed against suitable insect target genes in transgenic plants has been shown to give protection against pests through plant-mediated RNA interference（RNAi）.Thus,as a potential effective strategy for insect pest management in agricultural practice,RNAi for Mythimna separata control has received close attention in recent *** propose here to develop Mythimna separata-resistant plants using RNAi *** primary hypothesis is that if we can identify and deliver to Mythimna separata interfering RNAs that down-regulate expression of Mythimna separata genes such as Chitinase genes which are essential for their growth and development,they will result in a debilitating *** do this,we designed and carried out the following ***,using Gateway cloning technology to construct Plant binary expression vector corresponding for interfering sequences against the target genes chitinasel and *** rice expressing the hairpin dsRNAs was verified by southern blot and *** the transgenic rice was fed to mythimna separata,target Chitinase genes transcript levels were substantially decreased,also resulting in retarded larval growth and increased mortality.

关键词： Mythimna separata RNAi Chitinase gene transgenic rice

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Control of Mythimna Separata using RNAi technology

Tomato SIBL4 plays an important role in fruit pedicel organogenesis and abscission

全国农业生物化学与分子生物学第十五届学术研讨会

作者： Wenhua Bao hada wuriyanghan 内蒙古大学生命科学学院

Mythimna Separata is major agricultural pests that cause significant yield losses of crop plants each *** of double-stranded RNA(dsRNA) directed against suitable insect target genes in transgenic plants has been shown to give protection against pests through plant-mediated RNA interference(RNAi).Thus,as a potential effective strategy for insect pest management in agricultural practice,RNAi for Mythimna separata control has received close attention in recent *** propose here to develop Mythimna separata-resistant plants using RNAi *** primary hypothesis is that if we can identify and deliver to Mythimna separata interfering RNAs that down-regulate expression of Mythimna separata genes such as Chitinase genes which are essential for their growth and development,they will result in a debilitating *** do this,we designed and carried out the following *** used different sources of RNAi effectors to achieve knockdown of Mse-Chi genes and caused lethal *** rice expressing the hairpin dsRNAs was verified by southern blot and *** the transgenic rice was fed to Mythimna separata,target Chitinase genes transcript levels were substantially decreased,also resulting in retarded larval growth and increased mortality.

关键词： Mythimna separata RNAi Chitinase gene transgenic rice

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Horticulture Research 2021年第1期8卷 1094-1108页

作者： Fang Yan Zhehao Gong Guojian Hu Xuesong Ma Runyao Bai Ruonan Yu Qiang Zhang Wei Deng Zhengguo Li hada wuriyanghan Key Labo rato ry of Her bage&En demic Crop Biotech no logy Ministry of EducationSchool of Life ScienceInner Mongolia UniversityHohhot 010021China Key Lab oratory of Pla nt Hormones and Developme nt Regulati on of Chongqing School of Life SciencesChongqing University401331 ChongqingChina School of Biology and Basic Medical Sciences Soochow UniversitySuzhouChina Center of Plant Functional Genomics Institute of Advanced Interdisciplinary StudiesChongqing University401331 ChongqingChina

Abscission,a cell separation process,is an important trait that in fluences grain and fruit *** previously reported that BEL1-LIKE HOMEODOMAIN 4(SIBL4)is involved in chloroplast development and cell wall metabolism in tomato *** the present study,we showed that silencing SIBL4 resulted in the enlargement and pre-abscission of the tomato(Solanum lycopersicum ***-TOM)fruit *** anatomic analysis showed the presence of more epidermal cell layers and no obvious abscission zone(AZ)in the SlBL4 RNAi lines compared with the wild-type ***-seq analysis indicated that the regulation of abscission by SlBL4 was associated with the altered abundance of genes related to key meristems,auxin transporters,signaling components,and cell wall ***,SlBL4 positively affected the auxin concentration in the abscission zone.A dual-luciferase reporter assay revealed that SlBL4 activated the transcription of the JOINTLESS,OVATE,PIN1,and LAX3 *** reported a novel function ofSlBL4,which plays key roles in fruit pedicel organogenesis and abscission in tomatoes.

关键词： metabolism dic separation

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RNAi技术在植物抗病领域的应用研究进展

内蒙古大学学报（自然科学版） 2017年第4期48卷 445-451页

作者：哈达曹爱萍赵晨赵琪琦刁鹏飞内蒙古大学生命科学学院呼和浩特010021

RNAi是一种内源的基因沉默现象.由于其简便、高效及特异性,RNAi技术在各种模式和非模式生物系统中得到了广泛的应用.植物基因工程的宗旨是将动物、植物或微生物中分离到的目的基因,通过各种手段整合到植物基因组上,使其稳定遗传并赋予... 详细信息

RNAi是一种内源的基因沉默现象.由于其简便、高效及特异性,RNAi技术在各种模式和非模式生物系统中得到了广泛的应用.植物基因工程的宗旨是将动物、植物或微生物中分离到的目的基因,通过各种手段整合到植物基因组上,使其稳定遗传并赋予植物新的优良性状.RNAi技术在植物基因工程领域得到了广泛的应用,目前已经成功培育出了抗病毒、抗细菌、抗真菌、抗线虫转基因植物.2007年,美国Monsanto公司及中国科学院研究人员利用RNAi技术分别获得了抗虫转基因玉米和棉花,为农业害虫的防控提供了新的思路和方法.本文重点探讨RNAi技术的发展史及通过RNAi技术培育抗虫转基因植物的原理和途径.

关键词： RNAi 农业害虫抗病性转基因植物研究进展

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列当寄生紫苏的报道

内蒙古大学学报（自然科学版） 2019年第6期50卷 643-647页

作者：白润尧布拉嘎江正强哈达内蒙古大学生命科学学院牧草与特色作物生物技术教育部重点实验室

紫苏(学名:Perilla frutescens(L.)Britt.)是属于唇形科的一年生草本植物,具有特有的生物活性物质及营养价值,是我国首批公布的药食两用中草药资源之一.随着紫苏的温室培养及规模化种植,各种病害接踵而来.寄生植物从宿主获得营养物质,... 详细信息

紫苏(学名:Perilla frutescens(L.)Britt.)是属于唇形科的一年生草本植物,具有特有的生物活性物质及营养价值,是我国首批公布的药食两用中草药资源之一.随着紫苏的温室培养及规模化种植,各种病害接踵而来.寄生植物从宿主获得营养物质,导致宿主自身的生物量减少.寄生植物中列当科居多,而向日葵列当是向日葵的一大病害.本研究结果表明向日葵列当科寄生于紫苏的根部,显著影响其生长发育并降低其叶绿素含量,表明列当可能是紫苏种植中的一个潜在的病害.

关键词：紫苏列当寄生生长病害

乙烯对盐胁迫下紫花苜蓿种子萌发和幼苗生长的影响

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内蒙古大学学报（自然科学版） 2021年第1期52卷 59-69页

作者：王月于若男马雪松哈达孔令琪燕芳内蒙古大学生命科学学院牧草与特色作物生物技术教育部重点实验室呼和浩特010070 中国农业科学院草原研究所呼和浩特010021

紫花苜蓿是多年生草本豆科植物,其适口性好且富含蛋白质,但土壤盐渍化会影响其生长且产量降低.本研究使用不同浓度NaCl溶液模拟盐胁迫处理13份不同品种紫花苜蓿,筛选到最佳NaCl胁迫浓度为250mmol/L;NaCl胁迫后外源添加ETH和ACC,通过分... 详细信息

紫花苜蓿是多年生草本豆科植物,其适口性好且富含蛋白质,但土壤盐渍化会影响其生长且产量降低.本研究使用不同浓度NaCl溶液模拟盐胁迫处理13份不同品种紫花苜蓿,筛选到最佳NaCl胁迫浓度为250mmol/L;NaCl胁迫后外源添加ETH和ACC,通过分析紫花苜蓿发芽和苗长等指标,筛选到ETH和ACC的最佳处理浓度:0.5mmol/L和10μmol/L;在盐胁迫后外源添加ETH和ACC会缓解NaCl对发芽率、发芽势、发芽速度的抑制;初步证明乙烯参与调控了盐胁迫下紫花苜蓿种子萌发的过程。

关键词：紫花苜蓿盐胁迫乙烯种子萌发生理指标

Cloning and expression of putative ethylene receptor genes in soybean plant

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Progress in Natural Science:Materials International 2007年第10期17卷 1152-1160页

作者： Xie Zongming1,2,3, Lei Gang1,2, wuriyanghan hada1,2, Tian Aiguo1, Zhang Jinsong1 and Chen Shouyi1(1. National Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China 2. Graduate School of Chinese Academy of Sciences, Beijing 100039, China 3. Xinjiang Academy of Agricultural and Reclamation Science, Shihezi 832000, China) National Key Laboratory of Plant Genomics Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China Graduate School of Chinese Academy of Sciences Beijing 100039 China Xinjiang Academy of Agricultural and Reclamation Science Shihezi 832000 China

Ethylene plays important roles in plant growth, development, and stress responses, and ethylene receptors have been identified and studied extensively in various plant species. Here we report the cloning of four ethylene receptor genes from soybean, i.e. GmETR1, GmERS1, GmETR2 and GmEIN4. Construction of the phylogenic tree showed that GmETR1 and GmERS1 belong to subfamily I whereas GmETR2 and GmEIN4 belong to subfamily II. The four ethylene receptor genes showed different tissue-specific expression patterns in roots, stems, leaves, cotyledons, flowers, pods and seeds of soybean. These genes were differentially regulated by various abiotic stresses and plant hormones. The possible roles of the four genes in soybean plant were also discussed.

关键词： abiotic stress expression analysis ethylene receptor plant hormone soybean.