检索结果-南通市图书馆

A non-labeled DNA biosensor based on light addressable potentiometric sensor modified with TiO_2 thin film

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2009年第11期10卷 860-866页

作者： xiao-lin ZONG Chun-sheng wu xiao-ling wu Yun-feng LU Ping WANG Biosensor National Special Laboratory Key Laboratory of Biomedical Engineering of Education Ministry Department of Biomedical Engineering Zhejiang University Hangzhou 310027 China

Titanium dioxide (TiO2) thin film was deposited on the surface of the light addressable potentiometric sensor (LAPS) to modify the sensor surface for the non-labeled detection of DNA molecules. To evaluate the effect of ultraviolet (UV) treatment on the silanization level of TiO2 thin film by 3-aminopropyltriethoxysilane (APTS),fluorescein isothiocyanate (FITC) was used to label the amine group on the end of APTS immobilized onto the TiO2 thin film. We found that,with UV irradiation,the silanization level of the irradiated area of the TiO2 film was improved compared with the non-irradiated area under well-controlled conditions. This result indicates that TiO2 can act as a coating material on the biosensor surface to improve the effect and efficiency of the covalent immobilization of biomolecules on the sensor surface. The artificially synthesized probe DNA molecules were covalently linked onto the surface of TiO2 film. The hybridization of probe DNA and target DNA was monitored by the recording of I-V curves that shift along the voltage axis during the process of reaction. A significant LAPS signal can be detected at 10 μmol/L of target DNA sample.

关键词： DNA biosensor Titanium dioxide (Ti02) thin film Light addressable potentiometric sensor (LAPS) Silanization Fluorescein label Gene chip

KALMAN FILTERING CORRECTION IN REAL-TIME FORECASTING WITH HYDRODYNAMIC MODEL

在线全文

学校读者我要写书评

暂无评论

Journal of Hydrodynamics 2008年第3期20卷 391-397页

作者： wu xiao-ling WANG Chuan-hai CHEN Xi XIANG xiao-hua ZHOU Quan State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering Hohai University Nanjing210098 China Shanghai pudong area hydrology bureau Shanghai 201208 China

Accurate and reliable flood forecast is crucial for efficient real-time river management, including flood control, flood warning, reservoir operation and river regulation. In order to improve the estimate of the initial state of the forecasting system and to reduce the errors in the forecast period a data assimilation procedure was often need. The Kalman filter was proven to be an efficient method to adjust real-time flood series and improve the initial conditions before the forecast. A new model integrating the hydraulic model with the Kalman filter for real-time correction of flood forecast was developed and applied in the Three Gorges interzone of the Yangtze River. The method was calibrated and verified against the observed flood stage and discharge during Three Gorges Dam construction periods （2004）. The results demonstrate that the new model incorporates an accurate and fast updating technique can improve the reliability of the flood forecast.

关键词： real-time flood forecast Kalman filter hydraulic model alternate Kalman filtering method

Cloning of fatty acid elongase1 gene and molecular identification of A and C genome in Brassica species

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2007年第3期50卷 343-349页

作者： wu YuHua, xiao ling, wu Gang & LU ChangMing Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, wuhan 430062, China 1. Oil Crops Research Institute Chinese Academy of Agricultural Sciences Wuhan 430062 China

The fatty acid elongase 1 (FAE1) genes of Brassic napus were cloned from two cultivars, i.e. Zhong- shuan No. 9 with low erucic acid content, and Zhongyou 821 with high erucic acid content, using the degenerate PCR primers. The sequence analysis showed that there was no intron within the FAE1 genes. The FAE1 genes from Zhongyou 821 contained a coding sequence of 1521 nucleotides, and those cloned from Zhongshuan No. 9 contained a 1517 bp coding sequence. Alignment of the FAE1 sequences from Brassica rapa, B. oleracea and B. napus detected 31 single nucleotide polymorphic sites (2.03%), which resulted in 7 amino-acid substitutions. Further analysis indicated that 19 SNPs were genome-specific, of which, 95% were synonymous mutations. The nucleotide substitution at po- sition 1217 in the FAE1 genes led to a specific site of restricted cleavage. An AvrII cleavage site was present only in the C genome genes and absent in the A genome FAE1 genes. Digestion profile of the FAE1 sequences from B. rapa, B. oleracea and B. napus produced with AvrII confirmed that the FAE1 genes of B. oleracea origin was recognized and digested, while that of B. rapa origin could not. The results indicated that by AvrII cleavage it was possible to distinguish B. rapa from B. oleracea and be- tween the A and C genome of B. napus. In addition, the FAE1 genes could be used as marker genes to detect the pollen flow of B. napus, thus providing an alternative method for risk assessment of gene flow.

关键词： FAE acid Cloning of fatty acid elongase1 gene and molecular identification of A and C genome in Brassica species gene

Field mold stress induced catabolism of storage reserves in soybean seed and the resulting deterioration of seed quality in the field

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2022年第2期21卷 336-350页

作者： DENG Jun-cai LI xiao-man xiao Xin-li wu Hai-jun YANG Cai-qiong LONG Xi-yang ZHANG Qi-hui Nasir IQBAL WANG xiao-chun YONG Tai-wen DU Jun-bo YANG Feng LIU Wei-guo ZHANG Jing wu xiao-ling wu Yu-shan YANG Wen-yu LIU Jiang Sichuan Engineering Research Center for Crop Strip Intercropping System/Key Laboratory of Crop Ecophysiology and Farming System in Southwest Ministry of Agriculture and Rural AffairsChengdu 611130P.R.China College of Agriculture Sichuan Agricultural UniversityChengdu 611130P.R.China Institute of Ecological Agriculture Sichuan Agricultural UniversityChengdu 611130P.R.China College of Horticulture Sichuan Agricultural UniversityChengdu 611130P.R.China School of Agriculture Food and WineThe University of AdelaideUrrbraeSA 5064Australia

Excessive rainfall provides a favorable condition for field mold infection of plants, which triggers field mold(FM) stress. If FM stress occurs during the late maturation stage of soybean seed, it negatively affects seed yield and quality. To investigate the responses of soybean seed against FM stress and identify the underlying biochemical pathways involved, a greenhouse was equipped with an artificial rain producing system to allow the induction of mold growth on soybean seed. The induced quality changes and stress responses were revealed on the levels of both transcriptome and metabolome. The results showed that soybean seeds produced under FM stress conditions had an abnormal and inferior appearance, and also contained less storage reserves, such as protein and polysaccharide. Transcriptional analysis demonstrated that genes involved in amino acid metabolism, glycolysis, tricarboxylic acid, β-oxidation of fatty acids, and isoflavone biosynthesis were induced by FM stress. These results were supported by a multiple metabolic analysis which exhibited increases in the concentrations of a variety of amino acids, sugars, organic acids, and isoflavones, as well as reductions of several fatty acids. Reprogramming of these metabolic pathways mobilized and consumed stored protein, sugar and fatty acid reserves in the soybean seed in order to meet the energy and substrate demand on the defense system, but led to deterioration of seed quality. In general, FM stress induced catabolism of storage reserves and diminished the quality of soybean seed in the field. This study provides a more profound insight into seed deterioration caused by FM stress.

关键词： seed deterioration seed quality primary metabolism storage reserves resistance

A Novel Fluorinated Metal-organic Framework for Xenon/Krypton Separation

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Structural Chemistry 2021年第1期40卷 85-90,3页

作者： wu xiao-ling LI Zi-Jian TANG Fang-Dong QIAN Nan CHU Xin-Xin LIU Wei Shanghai Institute of Applied Physics Chinese Academy of ScienceShanghai 201800China Shanghai Institute of Measurement and Testing Technology Shanghai 201203China

A porous metal-organic framework[C21H20CoF6O7]was synthesized with Co(CH3 COO)2·4 H2 O and CPHFP(2,2’-bis(4-carboxyphenyl)hexafluoro-propane)under hydrothermal *** FMOF-Co has been characterized by elemental analysis,infrared spectroscopy,thermogravimetric analysis,single-crystal and powder X-ray *** crystal is of monoclinic space group P21/n with a=7.8911(2),b=29.9053(8),c=10.5811(3)Å,β=90.193(10)°,V=2499.70(12)Å^(3),Z=4,M_(r)=557.3,D_(c)=1.481 g·cm^(–3),F(000)=1132,μ=0.768 mm^(–1),GOOF=1.035,the final R=0.0722 and w R=0.2326 for 6347 observed reflections with I>2σ(I).The structure of FMOF-Co is constructed form the linkage of Co(II)through CPHFP,forming a 3 D net *** Kr and Xe uptake values are 0.225 and 0.484 mmol·g^(–1) at 303 K(100 KPa)with their mol selectivity to be 0.46;while the Kr and Xe uptake values are 0.486 and 0.077 mmol·g^(–1) at 233 K(100 KPa),with their mol separation being 6.29 at this condition.

关键词： fluorinated MOFs crystal structure gas adsorption

Shade adaptive response and yield analysis of different soybean genotypes in relay intercropping systems

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2017年第6期16卷 1331-1340页

作者： wu Yu-shan YANG Feng GONG Wan-zhuo Shoaib Ahmed FAN Yuan-fang wu xiao-ling YONG Tai-wen LIU Wei-guo SHU Kai LIU Jiang DU Jun-bo YANG Wen-yu College of Agronomy Sichuan Agricultural University Chengdu 611130 P.R. China Sichuan Engineering Research Center for Crop Strip Intercropping System Chengdu 611130 P.R. China Key Laboratory of Crop Ecophysiology and Farming System in Southwest Ministry of Agriculture Chengdu 611130 P.R. China Characteristic Crops Research Institute Chongqing Academy of Agricultural Sciences Chongqing 402160 P.R. China

Soybean is one of the major oil seed crops,which is usually intercropped with other crops to increase soybean production area and ***,soybean is highly sensitive to *** is unclear if soybean morphology responds to shading（i.e.,shade tolerance or avoidance）and which features may be suitable as screening materials in relay strip ***,in this study,various agronomic characteristics of different soybean genotypes were analyzed under relay intercropping *** soybean materials used in this study exhibited genetic diversity,and the coefficient of variations of the agronomic parameters ranged from 13.84 to 72.08%during the shade period and from 6.44 to 52.49%during the maturity *** ratios of shading to full irradiance in stem mass fraction（SMF）were almost greater than 1,whereas opposite results were found in the *** with full irradiance,the average stem length（SL）,leaf area ratio（LAR）and specific leaf area（SLA）for the two years（2013 and 2014）increased by 0.78,0.47 and 0.65 under shady conditions,***,the stem diameter（SD）,total biomass（TB）,leaf area（LA）,number of nodes（NN）on the main stem,and number of branches（BN）all *** the shady period,the SL and SMF exhibited a significant negative correlation with yield,and the SD exhibited a significant positive correlation with *** correlation between the soybean yield and agronomic parameters during the mature period,except for SL,the first pod height（FPH）,100-seed weight（100-SW）,and reproductive growth period（RGP）,were significant（P〈0.01）,especially for seed weight per branch（SWB）,pods per plant（PP）,BN,and vegetative growth period（VGP）.These results provide an insight into screening the shade tolerance of soybean varieties and can be useful in targeted breeding programs of relay intercropped soybeans.

关键词： intercropping light morphological parameters shade avoidance soybean

An experimental study of extracellular signal-regulated kinase and its interventional treatments in hepatic fibrosis

在线全文

学校读者我要写书评

暂无评论

Hepatobiliary & Pancreatic Diseases International 2008年第1期7卷 51-57页

作者： Jiang, Ming-De Zheng, Shu-Mei Xu, Hui Zeng, Wei-Zheng Zhang, Yong Sun, Hao-Ping Wang, Yun-Xia Qin, Jian-Ping wu, xiao-ling Gen Hosp Chenghu Milt Command Dept Gastroenterol Chengdu 610083 Peoples R China

BACKGROUND: The pathogenesis of hepatic fibrosis and cirrhosis is still not fully understood. The extracellular signal-regulated kinase (ERK) pathway is involved in the regulation of cell proliferation and differentiation. The aim of this study was to investigate the effects of PD98059, a specific inhibitor of ERK, on the cell cycle, cell proliferation, secretion of type I collagen and expression of cyclin D1 mRNA, CDK4 mRNA and transforming growth factor-beta 1 (TGF-beta 1) mRNA in rat hepatic stellate cells (HSCs) stimulated by acetaldehyde. METHODS: Rat HSCs stimulated by acetaldehyde were incubated with PD98059 at different concentrations. The cell cycle was analysed by flow cytometry. Cell proliferation was assessed by the methyl thiazolyl tetrazolium colorimetric assay. The mRNA expression of cyclin D1, CDK4 and TGF-beta 1 was examined using the reverse transcriptase-polymerase chain reaction. Type I collagen in the culture medium was detected by enzyme-linked immunosorbent assay. RESULTS: 20, 50 and 100 mu mol/L PD98059 significantly inhibited the proliferation and provoked a G0/G1-phase arrest of acetaldehyde-induced HSCs in a dose-dependent manner. The secretion of type I collagen and the expression of cyclin D1, CDK4 and TGF-beta 1 mRNA in acetaldehyde-induced HSCs were markedly inhibited by 50 and 100 mu mol/L PD98059, respectively. CONCLUSIONS: The ERK pathway regulates the cell proliferation, secretion of type I collagen and the expression of TGF-beta 1 mRNA in rat HSCs stimulated by acetaldehyde, which is likely related to its regulative effect on the cell cycle.

关键词： extracellular signal-regulated kinase hepatic stellate cell hepatic fibrosis

在线全文

学校读者我要写书评

暂无评论

实时校正中的旁侧入流反演方法

水科学进展 2009年第1期20卷 52-57页

作者：吴晓玲王船海向小华河海大学水文水资源与水利工程科学国家重点实验室江苏南京210098

在水文与水动力学相结合的预报模型中,对于水文模型计算有误差,造成水动力学区间旁侧入流与实际不吻合情况,依据水量平衡原理,对旁侧入流进行反演校正。采用四点线性隐格式求解圣维南方程组,结合卡尔曼滤波技术建立以水位、流量作为综... 详细信息

在水文与水动力学相结合的预报模型中,对于水文模型计算有误差,造成水动力学区间旁侧入流与实际不吻合情况,依据水量平衡原理,对旁侧入流进行反演校正。采用四点线性隐格式求解圣维南方程组,结合卡尔曼滤波技术建立以水位、流量作为综合状态量的卡尔曼滤波状态空间方程。采用反问题的思想把校正后的水位、流量不平衡量反馈到旁侧入流中,修正原有的区间来水量,进而修正预报期的旁侧入流。选择长江干流寸滩至万县河段,以及长江下游大通至镇江段进行演算,分别采用三峡水库135m蓄水阶段的水文资料和1998年水文资料进行6h洪水预报,结果表明旁侧入流反演校正方法是可行的。

关键词：实时校正反问题卡尔曼滤波旁侧入流

震后岷江上游地区生态旅游开发的SWOT分析

在线全文

学校读者我要写书评

暂无评论

软科学 2009年第7期23卷 68-71,82页

作者：吴小玲四川大学建筑与环境学院成都610065

通过采用SWOT法分析岷江上游地区生态旅游业开发的优势、劣势、机会和挑战,从而提出适宜的生态旅游业开发对策,更好地利用旅游资源,实现产业结构的优化升级,以促进灾后重建工作中生态旅游业的发展。

关键词：生态旅游 SWOT 战略对策灾后重建岷江上游