Cloning of fatty acid elongase1 gene and molecular identification of A and C genome in Brassica species

作     者：WU YuHua, XIAO Ling, WU Gang & LU ChangMing Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China 

作者机构：1. Oil Crops Research Institute Chinese Academy of Agricultural Sciences Wuhan 430062 China 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2007年第50卷第3期

页      面：343-349页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：the National Natural Science Foundation of China (Grant No. 30471099) Development Plan of the State Key Fundamental Research of China (Grant No. 2006CB101600) the National High Technology and Development Program of China (Grant No. 2006AA10A113) 

主　　题：FAE acid Cloning of fatty acid elongase1 gene and molecular identification of A and C genome in Brassica species gene 

摘      要：The fatty acid elongase 1 (FAE1) genes of Brassic napus were cloned from two cultivars, i.e. Zhong- shuan No. 9 with low erucic acid content, and Zhongyou 821 with high erucic acid content, using the degenerate PCR primers. The sequence analysis showed that there was no intron within the FAE1 genes. The FAE1 genes from Zhongyou 821 contained a coding sequence of 1521 nucleotides, and those cloned from Zhongshuan No. 9 contained a 1517 bp coding sequence. Alignment of the FAE1 sequences from Brassica rapa, B. oleracea and B. napus detected 31 single nucleotide polymorphic sites (2.03%), which resulted in 7 amino-acid substitutions. Further analysis indicated that 19 SNPs were genome-specific, of which, 95% were synonymous mutations. The nucleotide substitution at po- sition 1217 in the FAE1 genes led to a specific site of restricted cleavage. An AvrII cleavage site was present only in the C genome genes and absent in the A genome FAE1 genes. Digestion profile of the FAE1 sequences from B. rapa, B. oleracea and B. napus produced with AvrII confirmed that the FAE1 genes of B. oleracea origin was recognized and digested, while that of B. rapa origin could not. The results indicated that by AvrII cleavage it was possible to distinguish B. rapa from B. oleracea and be- tween the A and C genome of B. napus. In addition, the FAE1 genes could be used as marker genes to detect the pollen flow of B. napus, thus providing an alternative method for risk assessment of gene flow.