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Regulatory role of electroacupuncture on satellite glial cell activity in the colon and dorsal root ganglion of rats with irritable bowel syndrome

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Journal of Traditional Chinese Medicine 2024年第5期 981-990页

作者： ZHANG Fang YAN Cuina WENG Zhijun wu luyi QI Li ZHAO Min XIN Yuhu wu Huangan LIU Huirong Key Laboratory of Acupuncture and Immunological Effects Yueyang Hospital of Integrated Traditional Chinese and Western MedicineShanghai University of Traditional Chinese Medicine Shanghai Research Institute of Acupuncture and Meridian Shanghai University of Traditional Chinese Medicine Cancer Hospital Fudan University

OBJECTIVE: To investigate the role of satellite glial cells in irritable bowel syndrome(IBS) and the effect of electroacupuncture(EA) at the Tianshu(ST25) and Shangjuxu(ST37) combination. METHODS: A model for visceral hypersensitivity in IBS was induced through colorectal distension(CRD) stimulation. Clean-grade male Sprague-Dawley(SD) rats were randomly divided into four groups: a normal group(NG), a model group(MG), an electroacupuncture group(EA), and a glial cell inhibitor group(FCA). Bilateral EA(2/100 Hz, 1 mA, 30 min) was administered at the Tianshu(ST25) and Shangjuxu(ST37) in week 6. Abdominal withdrawal reflex(AWR) scores were used to assess the behavioral response associated with visceral hyperalgesia, while hematoxylin-eosin staining was employed to evaluate pathological changes in the colon. The protein and mRNA levels of glial fibrillary acidic protein(GFAP) in the colon and colon-related dorsal root ganglion(DRG) were analyzed using immun-ofluorescence, immunohistochemistry, Western blotting, real-time polymerase chain reaction. The impact of EA on electrophysiological properties of colon-related DRG neurons was observed through whole-cell patch clamp analysis. RESULTS: EA significantly reduced the visceral pain behavior scores in rats with IBS in response to graded(20, 40, 60, 80 mm Hg) CRD stimulation. Additionally, EA downregulated the protein and mRNA expression levels of GFAP in the colon and colon-related DRG of rats with IBS. EA also regulated the resting membrane potential, rheobase and action potential of colon-related DRG neurons in rats with IBS. CONCLUSIONS: EA can regulate the excitatory properties of colon-related DRG neurons by downregulating the protein and mRNA expression of GFAP in the colon and colon-related DRG, indicating a potential neurobiological mechanism by which EA relieves visceral hypersensitivity in rats with IBS.

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基于中性粒细胞胞外诱捕网研究隔药灸减轻克罗恩病大鼠结肠炎症的效应机制

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Journal of Acupuncture and Tuina Science 2024年第3期22卷 173-183页

作者：路驰徐静陆嫄吴璐一包春辉马喆钟蕊王照钦孙可鑫郑寒丹翁志军黄艳吴焕淦杨燕萍(译) Yueyang Hospital of Integrated Traditional Chinese and Western Medicine Shanghai University of Traditional Chinese MedicineShanghai 200437China Shanghai Research Institute of Acupuncture and Meridian Shanghai 200030China 不详

目的:从中性粒细胞胞外诱捕网(NETs)参与克罗恩病(CD)进展的角度,阐释隔药灸减轻CD大鼠肠道炎症的效应机制。方法:将大鼠随机分为正常组、模型组、隔药灸组和美沙拉嗪组。除正常组外,其余大鼠采用2,4,6-三硝基苯磺酸制备CD模型。正常组... 详细信息

目的:从中性粒细胞胞外诱捕网(NETs)参与克罗恩病(CD)进展的角度,阐释隔药灸减轻CD大鼠肠道炎症的效应机制。方法:将大鼠随机分为正常组、模型组、隔药灸组和美沙拉嗪组。除正常组外,其余大鼠采用2,4,6-三硝基苯磺酸制备CD模型。正常组和模型组不治疗仅做与其他组相同的固定。隔药灸组予以隔药灸气海和双侧天枢穴。美沙拉嗪组接受美沙拉嗪肠溶片灌胃治疗。比较各组大鼠一般情况,苏木精-伊红染色观察结肠组织病理学变化并评分。采用ABC酶联免疫吸附测定法检测各组大鼠血清中性粒细胞胞外诱捕网DNA(NETs-DNA)、中性粒细胞弹性蛋白酶DNA(NE-DNA)、过氧化物酶DNA(MPO-DNA)浓度,采用免疫荧光和实时定量聚合酶链反应技术观察各组大鼠结肠组织瓜氨酸化组蛋白3(citH3)、MPO和NE蛋白和mRNA的表达。结果:与正常组比较,模型组结肠黏膜溃疡深达肌层,上皮不完整,杯状细胞明显减少,可见大量炎性细胞浸润,结肠黏膜损伤指数(CMDI)评分显著增加(P<0.01),模型组血清NETs-DNA、NE-DNA、MPO-DNA浓度均增加(P<0.05),模型组结肠组织NE、citH3和MPO蛋白和mRNA表达均上调(P<0.01或P<0.05);与模型组相比,隔药灸组和美沙拉嗪组结肠黏膜上皮修复,杯状细胞明显增多,可见少量炎性细胞浸润,CMDI评分明显降低(P<0.01),隔药灸组和美沙拉嗪组血清NETs-DNA、NE-DNA、MPO-DNA浓度均下降(P<0.05),隔药灸组和美沙拉嗪组NE、citH3和MPO蛋白和mRNA表达下调(P<0.01或P<0.05)。结论:隔药灸能降低血清中性粒细胞NETs复合物的浓度,抑制结肠组织中的NETs复合物的蛋白和mRNA表达,可能是隔药灸缓解CD结肠黏膜炎症的机制之一。

关键词：隔药灸疗法药饼灸疗法炎症性肠病克罗恩病中性粒细胞胞外诱捕网

Graphitic carbon nitride nanosheets mitigate cadmium toxicity in Glycine max *** promoting cadmium retention in root and improving photosynthetic performance

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Journal of Environmental Sciences 2024年第5期139卷 543-555页

作者： Kai Xu Yunyu Guo Chenghua Xing Ronglong Fu Bin Zou Rongchuan Liu luyi Cai Jianfang Yan Xi-Lin wu Miaozhen Cai College of Geography and Environmental Sciences Zhejiang Normal UniversityJinhua 321004China College of Agriculture Jinhua PolytechnicJinhua 321007China

Cadmium(Cd)pollution poses a serious threat to plant growth and *** have shown great application potential for alleviation of Cd toxicity to *** this study,we applied graphitic carbon nitride nanosheets(g-C_(3)N_(4)NSs)for alleviation of Cd-toxicity to soybean(Glycine max L.).The g-C_(3)N_(4)NSs supplementation significantly improved plant growth and reduced oxidative damage in the Cd-toxicated soybean seedlings through hydroponic ***,the g-C_(3)N_(4)NSs dynamically regulated the root cell wall(RCW)components by increasing pectin content and modifying its demethylation via enhancing pectin methylesterase(PME)activity,therefore greatly enhanced stronger RCW-Cd retention(up to 82.8%)and reduced Cd migration to the ***,the g-C_(3)N_(4)NSs reversed the Cd-induced chlorosis,increased photosynthetic efficiency because of enhancement in F_v/F_mration,Y(Ⅱ)and sugars *** results provide new insights into the alleviation of Cd toxicity to plants by g-C_(3)N_(4)NSs,and shed light on the application of low-cost and environmental-friendly carbon-based NMs for alleviating heavy metal toxicity to plants.

关键词： Cadmium Carbon nitride nanosheets Cell wall Pectin Pectin methylesterase Soybean

Effects of electroacupuncture and Chinese kidney-nourishing medicine on polycystic ovary syndrome in obese patients

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Journal of Traditional Chinese Medicine 2013年第3期33卷 287-293页

作者： Liqing Yu Yanjun Liao Huangan wu Jimeng Zhao luyi wu Yin Shi Jianqiao Fang Department of Acupuncture and Moxibustion Shanghai Tianshan Hospital of Traditional Chinese Medicine Department of Medical Clinic Shanghai Institute of Acupuncture-Moxibustion and Meridians The Third Clinical College of Zhejiang Chinese Medical University

OBJECTIVE： To explore the effect of electroacu- puncture and Chinese kidney-nourishing medicine on insulin （INS）, adiponectin （APN）, leptin （LEP）, and glucolipid metabolism of obese patients with polycystic ovary syndrome （PCOS）. METHODS： Sixty-seven obese PCOS patients were randomly divided into two groups. Thirty-three pa- tients in the acupuncture-medicine group were treated three times a week with electroacupunc- ture at the Tianshu （ST 25）, Zhongwan （CV ] 2）, Qi- hai （CV 6）, Sanyinjiao （SP 6）, Geshu （BL 17）, and Cil-iao （BL 32） acupoints. They drug, Tiankui capsule, for 3 treatment. Point-taking and also took the Chinese months as a course of treatment in the elec- troacupuncture group of 34 patients was the same as those in the acupuncture-medicine group. We observed and compared the changes in the obesi- ty-related indexes of body weight （BW）, body mass index （BMI）, and waist-hip ratio （WHR）, as well as fasting plasma glucose （FPG）, fasting insulin （FINS）, APN, and LER RESULTS： BW, BMI, WHR, and FINS decreased and insulin sensitivity index （ISI） and APN were higher in the acupuncture-medicine group than in the electroacupuncture group （P〈0.01）. There was no obvious difference in LEP between the two groups （P〉0.05）. CONCLUSION： Acupuncture combined with medi- cine is better than just electroacupuncture for obese PCOS patients by improving obesity-related indexes, insulin sensitivity, and APN level. This indi- cates that acupuncture-medicine therapy is worth clinical popularization.

关键词： Electroacupuncture Reinforcing kid-ney Polycystic ovary syndrome Obesity Insulin Adiponectin Leptin

Low-loss and polarization insensitive 32×4 optical switch for ROADM applications

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Light(Science & Applications) 2024年第5期13卷 875-884页

作者： Xiaotian Zhu Xiang Wang Yanlu Huang Liyan wu Chunfei Zhao Mingzhu Xiao luyi Wang Roy Davidson Yanni Ou Brent E.Little Sai T.Chu Department of Physics City University of Hong KongTat Chee AvenueHong KongChina. QXP Technologies Inc. NO.15th Shanglinyuan 1st RoadXi’anShaanxi 710119China. State Key Laboratory of Information of Photonics and Optical Communications School of Electronic EngineeringBeijing University of Posts and TelecommunicationsBeijing 100876China

Integrated switches play a crucial role in the development of reconfigurable optical add-drop multiplexers(ROADMs)that have greater flexibility and compactness,ultimately leading to robust single-chip *** decades of research on switches with various structures and platforms,achieving a balance between dense integration,low insertion loss(IL),and polarization-dependent loss(PDL)remains a significant *** this paper,we propose and demonstrate a 32×4 optical switch using high-index doped silica glass(HDSG)for ROADM *** switch is designed to route any of the 32 inputs to the express ports or drop any channels from 32 inputs to the target 4 drop ports or add any of the 4 ports to any of the 32 express *** switch comprises 188 MachZehnder Interferometer(MZI)type switch elements,88 optical vias for the 44 optical bridges,and 618 waveguidewaveguide crossings with three-dimensional(3D)*** 1550 nm,the fiber-to-fiber loss for each express channel is below 2 dB,and across the C and L bands,below 3 *** each input channel to all 4 drop/add channels at 1550 nm,the loss is less than 3.5 dB and less than 5 dB across the C and L *** PDLs for all express and input channels to the 4 drop/add channels are below 0.3 dB over the C band,and the crosstalk is under−50 dB for both the C and L bands.

关键词： polarization ROADM optical

Impaired Spatial Firing Representations of Neurons in the Medial Entorhinal Cortex of the Epileptic Rat Using Microelectrode Arrays

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Research 2024年第2期2023卷 541-552页

作者： Zhaojie Xu Fan Mo Gucheng Yang Penghui Fan Botao Lu Wei Liang Fanli Kong luyi Jing Wei Xu Juntao Liu Mixia Wang Yirong wu Xinxia Cai State Key Laboratory of Transducer Technology Aerospace Information Research InstituteChinese Academy of SciencesBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China

Epilepsy severely impairs the cognitive behavior of *** remains unclear whether epilepsy-induced cognitive impairment is associated with neuronal activities in the medial entorhinal cortex(MEC),a region known for its involvement in spatial *** explore this neural mechanism,we recorded the spikes and local field potentials from MEC neurons in lithium-pilocarpine-induced epileptic rats using self-designed microelectrode *** the open field test,we identified spatial cells exhibiting spatially selective firing properties and assessed their spatial representations in relation to the progression of ***,we analyzed theta oscillations and theta modulation in both excitatory and inhibitory ***,we used a novel object recognition test to evaluate changes in spatial cognitive ability of epileptic *** the epilepsy modeling,the spatial tuning of various types of spatial cells had suffered a rapid and pronounced damage during the latent period(1 to 5 d).Subsequently,the firing characteristics and theta oscillations were *** the chronic period(>10 d),the performance in the novel object experiment *** conclusion,our study demonstrates the detrimental effect on spatial representations and electrophysiological properties of MEC neurons in the epileptic latency,suggesting the potential use of these changes as a"functional biomarker"for predicting cognitive impairment caused by epilepsy.

关键词： epilepsy impaired involvement

Effects of moxibustion on heat-shock protein 70 expression in the spinal cord and colonic mucosa in a rat model of ulcerative colitis

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Neural Regeneration Research 2010年第22期5卷 1717-1722页

作者： Li Qi Yin Shi luyi wu Jingping Mu Linying Tan Xiaopeng Ma Huirong Liu Shifen Xu Huangan wu Shanghai University of Traditional Chinese Medicine Shanghai 201203 China Key Laboratory of Acupuncture-moxibustion and Immunological Effects Shanghai Research Institute of Acupuncture and Meridian Shanghai 200030 China Department of Acupuncture Taihe Hospital Affiliated to Yunyang Medical College Shiyan 442000 Hubei Province China

Pathological changes in the colon are closely associated with the spinal cord, and innervation of spinal cord can regulate cellular functions. Our previous studies verified that moxibustion protects and restores the colonic mucosa, but the mechanisms of action remain unknown. The present study observed the effects of moxibustion and salicylazosulfapyridine on expression of heat-shock protein 70 （HSP70） and its mRNA in the spinal cord and colonic mucosa of ulcerative colitis rats. Results demonstrated that moxibustion and salicylazosulfapyridine increased HSP70 mRNA expression in the spinal cord and colonic mucosa of ulcerative colitis rats. The decreased transcriptional activity of HSP70 in the spinal cord and colonic mucosa might participate in damage to the colonic mucosa in ulcerative colitis rats. Moxibustion exerted protective effects on colonic mucosa by up-regulating HSP70 transcriptional activity in the spinal cord and colonic mucosa.

关键词： heat-shock protein 70 moxibustion spinal cord colonic mucosa ulcerative colitis

Electroacupuncture diminishes P2X_2 and P2X_3 purinergic receptor expression in dorsal root ganglia of rats with visceral hypersensitivity

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Neural Regeneration Research 2013年第9期8卷 802-808页

作者： Zhijun Weng luyi wu Yuan Lu Lidong Wang Linying Tan Ming Dong Yuhu Xin Shanghai Academy of Traditional Chinese Medicine Fudan University Shanghai Cancer Center

Electroacupuncture at Shangjuxu （ST37） and Tianshu （ST25） can improve visceral hypersensitivity in rats. Colorectal distension was used to establish a rat model of chronic visceral hypersensitivity. Immunohistochemistry was used to detect P2X2 and P2X3 receptor expression in dorsal root ganglia from rats with chronic visceral hypersensitivity. Results demonstrated that abdominal withdrawal reflex scores obviously increased following establishment of the model, indicating visceral hypersensitivity. Simultaneously, P2X2 and P2X3 receptor expression increased in dorsal root ganglia. After bilateral electroacupuncture at Shangjuxu and Tianshu, abdominal withdrawal reflex scores and P2X2 and P2X3 receptor expression decreased in rats with visceral hypersensitivity. These results indicated that electroacupuncture treatment improved visceral hypersensitivity in rats with irritable bowel syndrome by reducing P2X2 and P2X3 receptor expression in dorsal root ganglia.

关键词： neural regeneration acupuncture and moxibustion P2X2 P2X3 visceral hypersensitivity irritablebowel syndrome electroacupuncture P2 purinergic receptors abdominal withdrawal reflex scoresacupuncture and moxibustion peripheral nerve injury grants-supported paper photographscontaining paper neuroregeneration

Suspended moxibustion at Tianshu (ST25) inhibits prokineticin 1 and prokineticin receptor 1 expression in the spinal cord of rats with chronic visceral hypersensitivity

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Neural Regeneration Research 2012年第15期7卷 1145-1150页

作者： Chen Zhao Li Qit luyi wu Tao Yi Huangan wu Xinxin Guo Cili Zhou Huirong Liu Xiaomei Wang Yueyang Hospital of Integrated Traditional Chinese and Western Medicine Shanghai University of Traditional Chinese Medicine Shanghai201203 China Key Unit of Acupuncture-Moxibustion and Immunological Effects Shanghai University of Traditional Chinese Medicine Shanghai 200030China Qigong Institute Shanghai University of Traditional Chinese Medicine Shanghai 200030 China

Suspended moxibustion can decrease the expression of prokineticin 1 and its receptor in colonic tissue from rats modeling chronic visceral hyperalgesia. This study aimed to verify if rat spinal cord prokineticin 1 and its receptor contribute to the analgesic effect of suspended moxibustion in a rat model of irritable bowel syndrome where rats display chronic visceral hypersensitivity. Results showed that suspended moxibustion at Tianshu （ST25） point significantly decreased visceral sensitivity to colorectal distention in a chronic visceral hyperalgesia rat model; also protein and mRNA expression of prokineticin 1 and prokineticin receptor 1 in the spinal cord of rats was significantly decreased. Experimental findings indicate that prokineticin 1 and prokineticin receptor 1 are involved in the analgesia using suspended moxibustion in rats with chronic visceral

关键词： suspended moxibustion chronic visceral hypersensitivity irritable bowel syndrome prokineticin 1 prokineticin receptor 1 spinal cord neural regeneration