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Identification of genetic locus with resistance to take-all in the wheat-Psathyrostachys huashanica Keng introgression line H148

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Journal of Integrative Agriculture 2021年第12期20卷 3101-3113页

作者： BAI Sheng-sheng ZHANG Han-bing HAN Jing wu jian-hui LI Jia-chuang GENG Xing-xia LU Bo-ya XIE Song-feng HAN De-jun ZHAO Ji-xin YANG Qun-hui wu Jun CHEN Xin-hong Shaanxi Key Laboratory of Plant Genetic Engineering Breeding/College of Agronomy Northwest A&F UniversityYangling 712100P.R.China State Key Laboratory of Crop Stress Biology for Arid Areas/College of Agronomy Northwest A&F UniversityYangling 712100P.R.China Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement/College of Agronomy Northwest A&F UniversityYangling 712100P.R.China

Take-all is a devastating soil-borne disease of wheat(Triticum aestivum L.).Cultivating resistant line is an important measure to control this *** huashanica Keng is a valuable germplasm resource with high resistance to *** study reported on a wheat-/R huashanica introgression line H148 with improved take-all resistance compared with its susceptible parent *** elucidate the genetic mechanism of resistance in H148,the F_(2)genetic segregating population of H148×XN585 was *** mixed genetic model analysis showed that the take-all resistance was controlled by two major genes with additive,dominant and epistasis *** segregant analysis combined with wheat axiom 660K genotyping array analysis showed the polymorphic SNPs with take-all resistance from *** alien introgression were mainly distributed on the chromosome *** of the F_(2)population using the KASP marker mapped a major QTL in an interval of 68.8-70.1 Mb on ***-two genes were found in the target interval of the Chinese Spring reference genome *** to the functional annotation of genes,two protein genes that can improve the systematic resistance of plant roots were predicted as candidate *** development of *** introgression line H148 and the resistant QTL mapping information are expected to provide some valuable references for the fine mapping of disease-resistance gene and development of take-all resistant varieties through molecular marker-assisted selection.

关键词： wheat Psathyrostachys huashanica Keng take-all genetic analysis quantitative trait loci

Effect of Mono-2-ethyhexyl Phthalate on DNA Methylation in Human Prostate Cancer LNCaP Cells

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Biomedical and Environmental Sciences 2017年第9期30卷 641-648页

作者： wu jian hui CHEN Jiao WANG Yong XIA Bin WANG Rong ZHAO Yan WANG Qin Xia SONG Qi YAO Shun Heng ZHANG Yun hui SUN Zu Yue National Evaluation Centre for the Toxicology of Fertility Regulating Drug Department of Pharmacology and ToxicologyShanghai Institute of Planned Parenthood ResearchShanghai 200032China Key Laboratory of Reproduction Regulation of NPFPC Shanghai 200032China Reproductive and Developmental Research Institute of Fudan University ShanghaiShanghai 200032China Division of Health Risk Factors Surveillance and Control Shanghai Municipal Center for Disease Control and PreventionShanghai 200336China Key Laboratory of Public Health Safety Ministry of EducationSchool of Public HealthFudan UniversityShanghai 200032China

Objective To evaluate whether mono（2‐ethylhexyl） phthalate（MEHP） affects genomic DNA methylation and the methylation status of some specific genes such as patched gene（PTCH） and smoothened gene（SMO） in LNCaP cells. Methods LNCaP cells were treated with MEHP（0, 1, 5, 10, and 25 μmol/L） for 3 days. An ELISA assay was preformed to detect genomic methylation, including 5‐methylcytosine（5‐mC） and 5‐hydroxymethylcytosine（5‐hmC） content. A pyrosequencing assay was applied to assess DNA methylation in PTCH and SMO gene promoters. The correlation between DNA methylation and gene expression was assessed. Results The proportion of cytosines with 5‐mC methylation in LNCaP cells was significantly decreased by MEHP（1, 5, 10, and 25 μmol/L） in a dose‐dependent manner（P 〈 0.01）. For genes in the Hedgehog pathway, there was no significant MEHP concentration‐dependent difference in the DNA methylation of PTCH and SMO. Conclusion MEHP might affect the progression of prostate cancer through its effect on global DNA methylation.

关键词： Mono(2‐ethyhexyl) phthalate LNCaP Genomic Methylation PTCH SMO

Moving Objects Detection Using Intersecting Cortical Model in Enhanced Fish-eye Image

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Semiconductor Photonics and Technology 2009年第2期15卷 105-110,129页

作者： wu jian-hui YANG Kun-tao DU jian-rong ZHANG Nan-yang-sheng Dept. of Physics & Electronics Information Hunan Institute of Science & Technology Yueyang 414006 CHN College of Optoelectronics Science & Engineering Huazhong University of Science & Technology Wuhan 430074 CHN

A new method of the moving objects detection using the enhanced fish-eye lens and the intersecting cortical model （ICM） algorithm is proposed. The improved fish-eye lens is designed through controlling the entrance pupils of the lens. This lens has an ultra field of view about 183 degrees, and can image an ellipse picture on the 4 ： 3 rectangular CCD surface, which increases the CCD utilization and the image resolution. The ICM is a model based on pulse coupled neural network（PCNN） which is espeeially designed for image processing. It is derived from several visual cortex models and is basically the intersection of these models. The theoretical foundation of the ICM is given. An improved ICM algorithm in which some parameters are modified is used to detect moving objects specially. The experiment indicated that moving objects can be detected reliably and efficiently using ICM algorithm from the elliptical fish-eye image. It can be used in the field of traffic monitoring and other security domains.

关键词： enhanced fish-eye lens ICM PCNN motion detection

雨水口固体废弃物拦截和防臭气外逸技术的应用

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中国给水排水 2012年第18期28卷 14-18页

作者：吴坚慧上海市城市建设设计研究总院上海200125

为了削减随雨水排入河道的固体废弃物数量,保护水环境,提高雨水口机械化养护水平,防止从雨水口逸出的臭气干扰行人和居民的正常生活,提高城市空气质量,提出了在现有雨水口内安装用于收集雨水中的固体废弃物和防止臭气外逸的装置,介绍了... 详细信息

为了削减随雨水排入河道的固体废弃物数量,保护水环境,提高雨水口机械化养护水平,防止从雨水口逸出的臭气干扰行人和居民的正常生活,提高城市空气质量,提出了在现有雨水口内安装用于收集雨水中的固体废弃物和防止臭气外逸的装置,介绍了该装置的工作原理、构造、设计及组合,重点说明了装置的使用条件。工程实践表明,该装置的应用对环境保护起到了积极作用,具有经济效益和社会效益。

关键词：排水系统雨水口固体废弃物拦截防臭气外逸装置

“阐释之循环”之于古典诗词英译解读的方法论意义

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外语学刊 2011年第6期 105-108页

作者：吴慧坚广东第二师范学院广州510303

辨义在古典诗词翻译中尤显重要。钱钟书所论的"阐释之循环"为古典诗词英译释义与解读提供了科学的方法论指导。本文通过典型案例剖析,将传统语言学的训诂方法与现代语篇翻译理论相结合,阐明对语篇意义的解读应在认知关联性框架内"交互往... 详细信息

辨义在古典诗词翻译中尤显重要。钱钟书所论的"阐释之循环"为古典诗词英译释义与解读提供了科学的方法论指导。本文通过典型案例剖析,将传统语言学的训诂方法与现代语篇翻译理论相结合,阐明对语篇意义的解读应在认知关联性框架内"交互往复"地进行。

关键词：阐释之循环训诂关联性语境融合

Atmosphere Transmission Performance for 0.4 μm～0.8 μm Ray Radiation in Middle Latitude of China

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Semiconductor Photonics and Technology 2009年第1期15卷 34-40页

作者： wu jian-hui YANG Kun-tao XIANG Qiao-lian ZHANG Nan-yang College of Optoelectronics Science & Engineering Huazhong University of Science & Technology Wuhan 430074 CHN

This paper researched on the atmospheric transmission performance of 0.4 μm～0.8 μm ray radiation based on the characteristic of the middle latitude atmosphere in *** analysis of the characteristic of the actual atmosphere,the absorption of molecular and the scattering of the steam and ozone,as well as the aerosol scattering(big granule scattering) are play a leading role to the 0.4 μm～0.8 μm ray *** a better atmospheric transmission formula in horizontal path has been *** result of computer simulation indicates that this equation can best calculate the transmission performance of 0.4 μm～0.8 μm visible radiation in the middle latitude area of *** computing result was applied to the nuclear explosion parameter detection system based on 0.4 μm～0.8 μm visible *** nuclear explosion simulator to produce ray radiation,the tested result indicates that this method has the better measuring precision than the traditional method with the software of *** calculation result of this formula not only can apply directly to each kind of optoelectronics detecting system,but also to the optical wireless communication system based on the 0.4 μm～0.8 μm ray radiation.

关键词： middle latitude atmosphere atmospheric transmission radiation detection absorption scattering

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流式细胞术最新进展及临床应用

中国免疫学杂志 2019年第10期35卷 1271-1276页

作者：程小艳武会娟北京市理化分析测试中心北京市基因测序与功能分析工程技术中心

近年来,流式细胞术作为临床检验中无可替代的重要技术之一,在定量方式、检测内容、多参数分析、细胞成像、体内监测及数据分析等方面均取得了许多突破,应用日趋广泛,前景可观。本文将介绍流式细胞术在定量流式细胞术、多色流式细胞术、... 详细信息

近年来,流式细胞术作为临床检验中无可替代的重要技术之一,在定量方式、检测内容、多参数分析、细胞成像、体内监测及数据分析等方面均取得了许多突破,应用日趋广泛,前景可观。本文将介绍流式细胞术在定量流式细胞术、多色流式细胞术、磷酸化流式细胞术、质谱流式细胞术、成像流式细胞术及体内流式细胞术方面的最新进展及临床应用。

关键词：定量流式细胞术多色流式细胞术磷酸化流式细胞术质谱流式细胞术成像流式细胞术体内流式细胞术

Si对明弧堆焊合金M_7C_3相及耐磨性的影响

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材料工程 2017年第4期45卷 34-40页

作者：田兵龚建勋刘江晴吴慧剑湘潭大学机械工程学院湖南湘潭411105

采用药芯焊丝自保护明弧焊方法制备Fe-17Cr-4C-2V-Mn-Si-Ti多元合金系堆焊合金。借助金相显微镜、X射线衍射仪及扫描电镜等手段,研究硅对其M_7C_3(M=Fe,Cr,V,Mn)相及耐磨性的影响。结果表明:当硅含量从0.6%(质量分数,下同)增加到2.4%时... 详细信息

采用药芯焊丝自保护明弧焊方法制备Fe-17Cr-4C-2V-Mn-Si-Ti多元合金系堆焊合金。借助金相显微镜、X射线衍射仪及扫描电镜等手段,研究硅对其M_7C_3(M=Fe,Cr,V,Mn)相及耐磨性的影响。结果表明:当硅含量从0.6%(质量分数,下同)增加到2.4%时,初生M_7C_3相由板条状转变为块状弥散分布;其相邻间隔的γ-Fe数量逐渐减少直至消失。硅改变了初生M_7C_3形核前驱体-液态合金化高碳原子团簇的属性而引起其形态、分布和尺寸改变。磨损结果表明,当硅含量从0.6%增加至2.4%时,合金耐磨性先提高后下降,至1.5%时耐磨性最佳,微切削和微观断裂两种磨损机理并存。

关键词：明弧堆焊多元显微组织耐磨性

基于Booth算法的32×32乘法器IP核设计

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电子器件 2005年第1期28卷 218-220,234页

作者：汤晓慧杨军吴艳吴建辉东南大学国家专用集成电路系统工程研究中心南京210096

在Booth算法的基础上,提出了一个适用于多媒体加速单元(Multimedia Accelerator) 的乘法器IP核设计。通过增加一位符号位,本设计支持32×32无符号和有符号乘法。通过一个32×9结合2 bit Booth算法阵列乘法器循环四次加法,完成32bit乘法... 详细信息

在Booth算法的基础上,提出了一个适用于多媒体加速单元(Multimedia Accelerator) 的乘法器IP核设计。通过增加一位符号位,本设计支持32×32无符号和有符号乘法。通过一个32×9结合2 bit Booth算法阵列乘法器循环四次加法,完成32bit乘法。前四个时钟周期,每次处理一个9bit乘法,后两个周期分别处理低32 bit和高32 bit加法。我们采用2.5 V, 0.25μm SMIC CMOS工艺,实现乘法器的设计,其中部分积求和部分和ALU单元,Hspice仿真的最大延迟分别为0.64 ns,1.51 ns。

关键词：乘法器 IP核 Booth算法