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In-depth analysis of the influence of bio-silica filler(Didymosphenia geminata frustules)on the properties of Mg matrix composites

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Journal of Magnesium and Alloys 2023年第8期11卷 2853-2871页

作者： Izabela B.Zgłobicka Anna Dobkowska Aleksandra Zielińska Ewa Borucinska Mirosław J.Kruszewski RafałZybała Tomasz Płociński Joanna Idaszek Jakub Jaroszewicz Krystian Paradowski Bogusława Adamczyk-Cieślak Kostiantyn Nikiforow Bartosz Bucholc wojciechświęszkowski Krzysztof J.Kurzydłowski Faculty of Mechanical Engineering Bialystok University of TechnologyWiejska 45C15-351 BialystokPoland Faculty of Materials Science and Engineering Warsaw University of TechnologyWoloska 14102-507 WarsawPoland Lukasiewicz Research Network–Institute of Microelectronics and Photonics Al.Lotnikow 32/4602-668 WarsawPoland Institute of Physical Chemistry Polish Academy of Sciences Kasprzaka 44/5201-224 WarsawPoland Institute of Fundamental Technological Research Polish Academy of Sciences Pawinskiego 5B02-106WarsawPoland

A novel metal matrix composites(MMC)with Mg matrix reinforced with natural filler in the form of Didymosphenia geminata frustules(algae with distinctive siliceous shells)are presented in this *** plasma sintering(PPS)was used to manufacture Mg-based composites with 1,5 and 10 vol.%ceramic *** a reference,pure Mg was *** results show that the addition of 1 vol.%Didymosphenia geminata frustules to the Mg matrix increases its corrosion resistance by supporting passivation reactions,and do not affect the morphology of L929 *** of 5 vol.%the filler does not cause cytotoxic effects,but it supports microgalvanic reactions leading to the greater corrosion *** content than 5 vol.%the filler causes significant microgalvanic corrosion,as well as increases cytotoxicity due to the greater micro-galvanic effect of the composites containing 10 and 15 vol.%*** results of contact angle measurements show the hydrophilic character of the investigated materials,with slightly increase in numerical values with addition of amount of ceramic *** addition of Didymosphenia geminata frustules causes changes in a thermo-elastic properties such as mean apparent value of coefficient of thermal expansion(CTE)and thermal conductivity(λ).The addition of siliceous reinforcement resulted in a linear decrease of CTE and reduction in thermal conductivity over the entire temperature *** the increasing addition of Didymosphenia geminata frustules,an increase in strength with a decrease in compressive strain is *** all composites an increase in microhardness was *** results clearly indicate that filler in the form of Didymosphenia geminata frustules may significantly change the most important properties of pure Mg,indicating its wide potential in the application of Mg-based composites with a special focus on biomedical use.

关键词： Metal-matrix composites(MMCs) Pulse plasma sintering(PPS) Ceramic filler Microstructure Properties

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Modern approaches for detection of volatile organic compounds in metabolic studies focusing on pathogenic bacteria:Current state of the art

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Journal of Pharmaceutical Analysis 2024年第4期14卷 483-505页

作者： Karolina Zuchowska wojciech Filipiak Department of Pharmacodynamics and Molecular Pharmacology Faculty of PharmacyCollegium Medicum in BydgoszczNicolaus Copernicus University in Torun85-089 BydgoszczPoland

Pathogenic microorganisms produce numerous metabolites,including volatile organic compounds(VOCs).Monitoring these metabolites in biological matrices(e.g.,urine,blood,or breath)can reveal the presence of specific microorganisms,enabling the early diagnosis of infections and the timely implementation of tar-geted ***,complex matrices only contain trace levels of VOCs,and their constituent com-ponents can hinder determination of these ***,modern analytical techniques enabling the non-invasive identification and precise quantification of microbial VOCs are *** this paper,we discuss bacterial VOC analysis under in vitro conditions,in animal models and disease diagnosis in humans,including techniques for offline and online analysis in clinical *** also consider the advantages and limitations of novel microextraction techniques used to prepare biological samples for VOC analysis,in addition to reviewing current clinical studies on bacterial volatilomes that address inter-species in-teractions,the kinetics of VOC metabolism,and species-and drug-resistance specificity.

关键词： Volatile organic compounds Pathogenic bacteria metabolites Metabolomics Microextraction techniques Gas chromatography-mass spectrometry In vivo breath analysis In vitro model

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Effect of Eu3+,Bi3+,and Li+ doping on luminescent property of GdNbO4

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Journal of Rare Earths 2024年第1期42卷 66-75页

作者： S.W.wi Y.S.Lee Department of Physics and Integrative Institute of Basic Sciences Soongsil University

We investigated the emission properties of Eu3+-doped GdNbO4-based oxide phosphors,aiming at improving the Eu3+emission by codoping of Bi3+and Li+.Eu3+singly doped,Eu3+and Bi3+doubly doped,and Eu3+,Bi3+,and Li+triply doped GdNbO4were prepared using a high-temperature solid-state reaction *** red-orange emissions from Eu3+in the singly doped samples are significantly improved by the codoping of Bi3+,mainly due to the energy transfer from Bi3+to Eu3+.The additional codoping of Li+is found to increase the Eu3+emission *** improvement might be attributed to the increase in the photoluminescent quantum yield originating from larger grain sizes and better crystallinity.A detailed analysis of the X-ray diffraction pattern and the asymmetric ratio estimated from the photoluminescence spectra show that the local lattice environment around Eu3+in GdNbO4does not change significantly with the codoping of Bi3+and Li+.Our results indicate that Li+doping is a promising way to improve the emission properties of rare-earth ion-doped GdNbO4phosphors.

关键词： Eu 3+ GdNbO 4 Codoping of Li + Photoluminescence Sensitizer Rare earths

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Effect of Eu^(3+),Bi^(3+),and Li^(+) doping on luminescent property of GdNbO_(4)

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Journal of Rare Earths 2024年第1期42卷 66-75,I0003页

作者： S.W.wi Y.S.Lee Department of Physics and Integrative Institute of Basic Sciences Soongsil UniversitySeoul06978Republic of Korea

We investigated the emission properties of Eu^(3+)-doped GdNbO_(4)-based oxide phosphors,aiming at improving the Eu^(3+)emission by codoping of Bi^(3+)and Li^(+).Eu^(3+)singly doped,Eu^(3+)and Bi^(3+)doubly doped,and Eu^(3+),Bi^(3+),and Li^(+)triply doped GdNbO_(4)were prepared using a high-temperature solid-state reaction *** red-orange emissions from Eu^(3+)in the singly doped samples are significantly improved by the codoping of Bi^(3+),mainly due to the energy transfer from Bi^(3+)to Eu^(3+).The additional codoping of Li^(+)is found to increase the Eu^(3+)emission *** improvement might be attributed to the increase in the photoluminescent quantum yield originating from larger grain sizes and better crystallinity.A detailed analysis of the X-ray diffraction pattern and the asymmetric ratio estimated from the photoluminescence spectra show that the local lattice environment around Eu^(3+)in GdNbO_(4)does not change significantly with the codoping of Bi^(3+)and Li^(+).Our results indicate that Li^(+)doping is a promising way to improve the emission properties of rare-earth ion-doped GdNbO_(4)phosphors.

关键词： Eu^(3+) GdNbO_(4) Codoping of Li^(+) Photoluminescence Sensitizer Rare earths

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Microfluidic bubble-generator enables digital light processing 3D printing of porous structures

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Aggregate 2024年第1期5卷 280-291页

作者： Philipp Weber Ling Cai Francisco Javier Aguilar Rojas Carlos Ezio Garciamendez-Mijares Maria Celeste Tirelli Francesco Nalin Jakub Jaroszewicz wojciech Swi eszkowski Marco Costantini Yu Shrike Zhang Division of Engineering in Medicine Department of MedicineBrigham and Women’s HospitalHarvard Medical SchoolCambridgeMassachusettsUSA Institute of Physical Chemistry—Polish Academy of Sciences KasprzakaPoland Biomaterials Group Materials Design DivisionFaculty of Materials Science and EngineeringWarsaw University of TechnologyWoloskaPoland

Three-dimensional(3D)printing is an emerging technique that has shown promising success in engineering human tissues in recent *** development of vatphotopolymerization printing modalities has significantly enhanced the complexity level for 3D printing of various functional structures and ***,the development of microfluidic chip systems is an emerging research sector with promising medical *** work demonstrates the coupling of a digital light processing(DLP)printing procedure with a microfluidic chip system to produce size-tunable,3D-printable porosities with narrow pore size distributions within a gelatin methacryloyl(GelMA)hydrogel *** is found that the generation of size-tunable gas bubbles trapped within an aqueous GelMA hydrogel-precursor can be controlled with high ***,the porosities are printed in two-dimensional(2D)as well as in 3D using the DLP *** addition,the cytocompatibility of the printed porous scaffolds is investigated using fibroblasts,where high cell viabilities as well as cell proliferation,spreading,and migration are *** is anticipated that the strategy is widely applicable in a range of application areas such as tissue engineering and regenerative medicine,among others.

关键词： 3D printing biofabrication bubble digital light processing microfluidics porous

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Targeting miRNA by CRISPR/Cas in cancer:advantages and challenges

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Military Medical Research 2024年第3期11卷 345-373页

作者： Bashdar Mahmud Hussen Mohammed Fatih Rasul Snur Rasool Abdullah Hazha Jamal Hidayat Goran Sedeeq Hama Faraj Fattma Abodi Ali Abbas Salihi Aria Baniahmad Soudeh Ghafouri-Fard Milladur Rahman Mark C.Glassy wojciech Branicki Mohammad Taheri Department of Biomedical Sciences Cihan University-ErbilErbilKurdistan Region 44001Iraq Department of Clinical Analysis College of PharmacyHawler Medical UniversityErbilKurdistan Region 44001Iraq Department of Pharmaceutical Basic Science Faculty of PharmacyTishk International UniversityErbilKurdistan Region 44001Iraq Medical Laboratory Science Lebanese French UniversityErbilKurdistan Region 44001Iraq Department of Biology College of EducationSalahaddin University-ErbilErbilKurdistan Region 44001Iraq Department of Medical Laboratory Science Komar University of Science and TechnologySulaymaniyah 46001Iraq Department of Medical Microbiology College of Health SciencesHawler Medical UniversityErbilKurdistan Region 44001Iraq Department of Biology College of ScienceSalahaddin University-ErbilErbilKurdistan Region 44001Iraq Center of Research and Strategic Studies Lebanese French UniversityErbil 44001Iraq Institute of Human Genetics Jena University Hospital07747 JenaGermany Department of Medical Genetics School of MedicineShahid Beheshti University of Medical SciencesTehran 374-37515Iran Department of Clinical Sciences MalmöSection for SurgeryLund University22100 MalmöSweden Translational Neuro-Oncology Laboratory San Diego(UCSD)Moores Cancer CenterUniversity of CaliforniaSan DiegoCA 94720USA Faculty of Biology Institute of Zoology and Biomedical ResearchJagiellonian University31-007 KrakówPoland Urology and Nephrology Research Center Shahid Beheshti University of Medical SciencesTehran 374-37515Iran

Clustered regulatory interspaced short palindromic repeats(CRISPR)has changed biomedical research and provided entirely new models to analyze every aspect of biomedical sciences during the last *** the study of cancer,the CRISPR/CRISPR-associated protein(Cas)system opens new avenues into issues that were once unknown in our knowledge of the non-coding genome,tumor heterogeneity,and precision ***/Cas-based geneediting technology now allows for the precise and permanent targeting of mutations and provides an opportunity to target small non-coding RNAs such as microRNAs(miRNAs).However,the development of effective and safe cancer gene editing therapy is highly dependent on proper design to be innocuous to normal cells and prevent introducing other *** study aims to highlight the cutting-edge approaches in cancer-gene editing therapy based on the CRISPR/Cas technology to target miRNAs in cancer ***,we highlight the potential challenges in CRISPR/Cas-mediated miRNA gene editing and offer advanced strategies to overcome them.

关键词： CRISPR CRISPR/Cas9 CRISPR/Cas12 Gene editing miRNAs Cancer therapy

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Impact of systemic steroids combined with immunosuppressive treatment on glaucomatous features in patients with systemic lupus erythematosus

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International Journal of Ophthalmology(English edition) 2022年第1期15卷 71-76页

作者： Marta P.wiącek Danuta Bobrowska–Snarska Marek Brzosko wojciech Lubiński Monika Modrzejewska First Department of Ophthalmology Pomeranian Medical UniversitySzczecin 70-111Poland Institute of Physics University of SzczecinSzczecin 70-451Poland Department of Rheumatology Internal Medicine and GeriatricsPomeranian Medical UniversitySzczecin 71-252Poland Second Department of Ophthalmology Pomeranian Medical UniversitySzczecin 70-111Poland

AIM:To evaluate the incidence of increased intraocular pressure(IOP)and glaucomatous changes in systemic lupus erythematosus(SLE)patients in comparison with systemic steroids and immunosuppressive ***:Sixty-two women with SLE were divided into two groups:treated(n=47,94 eyes)and not treated(n=15,30 eyes)with systemic glucocorticosteroids(GC;GC-free).Twenty-one individuals in GC group were treated with immunosuppressive agents(immunomodulating and biologic).The visual acuity and IOP with ocular pulsatile amplitude(OPA)measurements,as well as scanning laser polarimetry(GDx)with nerve fiber index(NFI)measurement,spectral domain optical coherence tomography(SD-OCT)of the optic disk with retinal nerve fiber layer(RNFL)analysis and the macular region with ganglion cell analysis(GCA)were ***:Mean IOP values in group with combined GC and immunosuppressive therapy was 15.8±2.56 mm Hg and was significantly lower than in individuals with exclusive GC treatment(17.63±4.38 mm Hg,P=0.043).Contrary,no dif ferences in mean IOP values between GC-free group and individuals treated with combined GC and immunosuppressive therapy were detected(P=0.563).Similarly,mean IOP in GC was 17.14±3.94 mm Hg and in GC-free patients was equal to 16.67±3.45 mm Hg(P=0.671).According to treatment regimen no statistical differences in optic disk SD-OCT for RNFL thickness,RNFL symmetry,cupping volume and the C/D ratio were ***,no statistical differences for the mean and minimal ganglion cell layer(GCL)thickness measured in macular SD-OCT or NFI in GDx were ***:Combined immunosuppressive and systemic GC therapy in SLE patients may lower the risk of iatrogenic ocular *** relationship between treatment regimen and glaucomatous damage of optic nerve fibers in analyzed groups with SLE is detected.

关键词： immunosuppression ocular hypertension steroid induced glaucoma steroid therapy systemic lupus erythematosus

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In vitro and in vivo degradation behavior of Mg-0.45Zn-0.45Ca(ZX00)screws for orthopedic applications

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Bioactive Materials 2023年第10期28卷 132-154页

作者： Diana C.Martinez Anna Dobkowska Romy Marek Hanna Cwieka Jakub Jaroszewicz Tomasz Płocinski Crtomir Donik Heike Helmholz Berengere Luthringer-Feyerabend Berit Zeller-Plumhoff Regine willumeit-Romer wojciech Swięszkowski Biomaterials Group Materials Design DivisionFaculty of Materials Science and EngineeringWarsaw University of TechnologyWołoska 14102-507WarsawPoland Department of Orthopedics and Traumatology Medical University of GrazAuenbruggerplatz 58036GrazAustria Institute of Metallic Biomaterials Helmholtz-Zentrum Hereon GmbH21502GeesthachtGermany Department of Physics and Chemistry of Materials Institute of Metals and TechnologyUniversity of Ljubljan1Lepi Pot 11SI-1000Ljubljan1Slovenia

Magnesium (Mg) alloys have become a potential material for orthopedic implants due to their unnecessary implant removal, biocompatibility, and mechanical integrity until fracture healing. This study examined the in vitro and in vivo degradation of an Mg fixation screw composed of Mg-0.45Zn-0.45Ca (ZX00, in wt.%). with ZX00 human-sized implants, in vitro immersion tests up to 28 days under physiological conditions, along with electrochemical measurements were performed for the first time. In addition, ZX00 screws were implanted in the diaphysis of sheep for 6, 12, and 24 weeks to assess the degradation and biocompatibility of the screws in vivo. Using scanning electron microscopy (SEM) coupled with energy dispersive X-ray spectroscopy (EDX), micro-computed tomography (μCT), X-ray photoelectron spectroscopy (XPS), and histology, the surface and cross-sectional morphologies of the corrosion layers formed, as well as the bone-corrosion-layer-implant interfaces, were analyzed. Our findings from in vivo testing demonstrated that ZX00 alloy promotes bone healing and the formation of new bone in direct contact with the corrosion products. In addition, the same elemental composition of corrosion products was observed for in vitro and in vivo experiments;however, their elemental distribution and thicknesses differ depending on the implant location. Our findings suggest that the corrosion resistance was microstructure-dependent. The head zone was the least corrosion-resistant, indicating that the production procedure could impact the corrosion performance of the implant. In spite of this, the formation of new bone and no adverse effects on the surrounding tissues demonstrated that the ZX00 is a suitable Mg-based alloy for temporary bone implants.

关键词： Magnesium alloys Biodegradable implants Microstructure Electron microscopy Corrosion layers

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Investigation of the porous drag and permeability at the porous-fluid interface: Influence of the filtering parameters on Darcy closure

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Particuology 2023年第7期78卷 122-135页

作者： wojciech Sadowski Francesca di Mare Chair of Thermal Turbomachines and Aeroengines Department of Mechanical EngineeringRuhr University BochumUniversitatsstr.15044801Germany

The porous-fluid interface encompasses a region bridging the flow inside a porous medium and a free-flowing *** the context of volume-averaged simulations,it can be described by a set of gradually changing parameters defining the porous medium,mainly porosity and *** this paper,both the permeability and the porous-induced drag force are evaluated a-priori,by explicitly filtering a set of Particle-Resolved Simulations(PRS)of the flow in the channel partially occupied by the porous *** porous matrices are considered and the influence of the geometry and filtering parameters on the macroscopic quantities is ***,the focus is placed on the requirements for the kernel type and size to perform filtering accurately,and their impact on the distribution of permeability at the *** performance of the typically used models for the permeability is compared to the explicitly filtered ***,a new model for permeability and the drag force is introduced,taking into account the information about the fltering size and non-uniformity of the velocity *** model greatly improves the prediction of velocity at the porous-fluid interface and serves as a proof of concept that a successful porous drag model should strive to include information about both parameters.

关键词： Porous-fluid interface Explicit filtering Permeability Porous drag

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湘西苗绣艺术特点及当代传承

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丝网印刷 2024年第6期 35-37页

作者：刘佳怡吴芊曳宁波大学潘天寿建筑与艺术设计学院

湘西苗绣是融合地域性与民族性的中国传统文化代表之一,其图案不仅具有一定的原始性,而且做到了色彩上的“寒热”聚合。如今传统苗绣面临着艰难的生存语境。结合经典的案例,可以根据大众的消费心理需要,在图案应景意义和形式张力上进行... 详细信息

湘西苗绣是融合地域性与民族性的中国传统文化代表之一,其图案不仅具有一定的原始性,而且做到了色彩上的“寒热”聚合。如今传统苗绣面临着艰难的生存语境。结合经典的案例,可以根据大众的消费心理需要,在图案应景意义和形式张力上进行挖掘,从而促进其现代生命的蝶变。

关键词：湘西苗绣艺术特点当代传承现代设计

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