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No evidence of predator odor avoidance in a North American bird community

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Avian Research 2024年第1期15卷 133-138页

作者： Austin Dotta Batur Yaman Alex Van huynh Department of Biology DeSales University2755 Station AvenueCenter ValleyPAUSA

Recent advances in our understanding of avian chemical communication have highlighted the importance of olfaction in many aspects of avian *** studies investigating predator avoidance behaviors in response to predator odor cues have produced mixed results across species and *** we assess if a community of birds in eastern Pennsylvania displays avoidance behaviors towards predator odor cues in a natural foraging *** use clay caterpillars to measure foraging activity by birds in the presence of predator(bobcat)urine,non-predator(rabbit)urine,and water controls in two different environmental contexts(field ***).Although we detected a weak trend for birds to forage less at predator urine-treated sites,we found no significant difference in avian foraging between the site *** did find that foraging rates between environmental contexts changed significantly over the course of the experiment,with forest sites showing decreasing foraging rates and field sites showing increasing foraging *** results reinforce the published literature that avoidance of predator odors by birds may not be ubiquitous across contexts and species.

关键词： Foraging Odor Olfaction Predation Predator-avoidance

Improvement of hepatic fibrosis after tenofovir disoproxil fumarate switching to tenofovir alafenamide for three years

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World Journal of Hepatology 2024年第7期16卷 1009-1017页

作者： Tung huynh Delana MyAn Bui Tina Xiwen Zhou Ke-Qin Hu Department of Pharmacy University of California Irvine Medical CenterOrangeCA 92868United States University of Houston HoustonTX 77204United States Chicago Medical School at Rosalind Franklin University North ChicagoIL 60064United States Division of Gastroenterology and Hepatology University of California IrvineSchool of MedicineOrangeCA 92868United States

BACKGROUND Both tenofovir alafenamide(TAF)and tenofovir disoproxil fumarate(TDF)are the first-line treatments for chronic hepatitis B(CHB).We have showed switching from TDF to TAF for 96 weeks resulted in further alanine aminotransferase(ALT)improvement,but data remain lacking on the long-term benefits of TDF switching to TAF on hepatic *** To assess the benefits of TDF switching to TAF for 3 years on ALT,aspartate aminotransferase(AST),and hepatic fibrosis improvement in patients with *** A single center retrospective study on 53 patients with CHB who were initially treated with TDF,then switched to TAF to determine dynamic patterns of ALT,AST,AST to platelet ratio index(APRI),fibrosis-4(FIB-4)scores,and shear wave elastography(SWE)reading improvement at switching week 144,and the associated *** The mean age was 55(28-80);45.3%,males;15.1%,clinical cirrhosis;mean baseline ALT,24.8;AST,25.7 U/L;APRI,0.37;and FIB-4,*** 144 weeks TDF switching to TAF,mean ALT and AST were reduced to 19.7 and 21,*** baseline to switching week 144,the rates of ALT and AST60 mL/min was increased from 86.5%at baseline to 88.2%at switching week *** Our data confirmed that switching from TDF to TAF for 3 years results in not only persistent ALT/AST improvement,but also hepatic fibrosis improvement by APRI,FIB-4 scores,as well as SWE reading,the important clinical benefits of long-term hepatitis B virus antiviral treatment with TAF.

关键词： Tenofovir alafenamide Tenofovir disoproxil fumarate Switching Hepatic fibrosis improvement Aspartate aminotransferase to platelet ratio index Fibrosis-4 Shear wave elastography

Prevalence and risk factors associated with long COVID symptoms in children and adolescents in a southern province of Vietnam

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Asian Pacific Journal of Tropical Medicine 2024年第3期17卷 119-128,I0001,I0002页

作者： huynh Ngoc Linh Nguyen The Tan Le Thi Minh Thu Nguyen Tu Loan Nguyen Thi To Uyen Le Thanh Thao Trang Truong Thanh Nam Doan Hoang Phu Faculty of Medicine Ca Mau Medical CollegeCa Mau 98000Vietnam Ca Mau Obstetrics and Pediatrics Hospital Ca Mau 98000Vietnam Faculty of Basic Science and Public Health Dong Thap Medical CollegeDong Thap 81000Vietnam Graduate University of Sciences and Technology Vietnam Academy of Science and TechnologyHa Noi 10000Vietnam Community Public Health Research Program School of Public HealthWalailak UniversityNakhon Si Thammarat 80160Thailand Faculty Public Health Can Tho University of Medicine and PharmacyCan Tho City 94000Vietnam Doctoral Program in Health Sciences College of Graduate StudiesWalailak UniversityNakhon Si Thammarat 80160Thailand

Objective:To investigate the prevalence and risk factors associated with long COVID symptoms among children and adolescents who have recovered from ***:This study applied a cross-sectional approach within community settings in a southern province of Vietnam.A structured questionnaire featuring socio-demographic information and common long COVID symptoms was *** correlation coefficients assessed associations among pairs of long COVID ***,multivariable logistic regression models were performed to investigate the risk factors of long COVID in recovered COVID-19 children and ***:Among 422 participants,39.3%reported long COVID symptoms,with a prevalence of 45.2%(SD=0.5)in children and 22.2%(SD=0.4)in *** symptoms reported were cough 34.6%(SD=0.5),fatigue 20.6%(SD=0.4),shortness of breath 10.9%(SD=0.3),and lack of appetite 6.6%(SD=0.3).Concerning risk factors of long COVID,a higher risk was observed among demographic groups,including girls(OR 1.25,95%CI 1.15-1.37;P<0.001,reference:boys),children compared to adolescents(OR 1.24,95%CI 1.12-1.37;P<0.001),overweight individuals(OR 1.14,95%CI 1.02-1.27;P=0.018,reference:healthy weight),and participants without any COVID-19 vaccination(OR 1.36,95%CI 1.20-1.54;P<0.001),or have received only one single dose(OR 1.35,95%CI 1.10-1.64;P=0.004)compared to those who have received two ***,patients with a COVID-19 treatment duration exceeding two weeks also had a higher risk of long COVID(OR 1.32,95%CI 1.09-1.60;P=0.003)than those who recovered less than seven ***:The insights from this study provide crucial guidance for predicting the factors associated with the occurrence of long COVID in pediatric patients,contributing to strategic interventions aimed at mitigating the long COVID risks among children and adolescents in Vietnam.

关键词： Long COVID Prevalence Risk factors Children Adolescent Vietnam

The neutrophil–osteogenic cell axis promotes bone destruction in periodontitis

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International Journal of Oral Science 2024年第1期16卷 154-162页

作者： Yutaro Ando Masayuki Tsukasaki Nam Cong-Nhat huynh Shizao Zang Minglu Yan Ryunosuke Muro Kazutaka Nakamura Masatsugu Komagamine Noriko Komatsu Kazuo Okamoto Kenta Nakano Tadashi Okamura Akira Yamaguchi Kazuyuki Ishihara Hiroshi Takayanagi Department of Immunology Graduate School of Medicine and Faculty of MedicineThe University of Tokyo7-3-1HongoBunkyo-kuTokyoJapan Department of Microbiology Tokyo Dental College2-1-14 Kanda-Misaki-choChiyoda-kuTokyoJapan Oral Health Science Center Tokyo Dental College2-9-18Kanda-Misaki-choChiyodakuTokyoJapan Department of Osteoimmunology Graduate School of Medicine and Faculty of MedicineThe University of Tokyo7-3-1HongoBunkyo-kuTokyoJapan Unit of Prosthodontics Laboratory of Oral-Maxillofacial Biology Faculty of Odonto-StomatologyUniversity of Medicine and Pharmacy at Ho Chi Minh CityHo Chi Minh CityVietnam Department of Oral and Maxillofacial Surgery Department of Sensory and Motor System MedicineGraduate School of MedicineThe University of TokyoTokyoJapan Division of Rheumatology Department of Internal MedicineKeio University School of MedicineTokyoJapan Department of Laboratory Animal Medicine Research InstituteNational Center for Global Health and MedicineTokyoJapan

The immune-stromal cell interactions play a key role in health and diseases. In periodontitis, the most prevalent infectious disease in humans, immune cells accumulate in the oral mucosa and promote bone destruction by inducing receptor activator of nuclear factor-κB ligand (RANKL) expression in osteogenic cells such as osteoblasts and periodontal ligament cells. However, the detailed mechanism underlying immune–bone cell interactions in periodontitis is not fully understood. Here, we performed single-cell RNAsequencing analysis on mouse periodontal lesions and showed that neutrophil–osteogenic cell crosstalk is involved in periodontitis-induced bone loss. The periodontal lesions displayed marked infiltration of neutrophils, and in silico analyses suggested that the neutrophils interacted with osteogenic cells through cytokine production. Among the cytokines expressed in the periodontal neutrophils, oncostatin M (OSM) potently induced RANKL expression in the primary osteoblasts, and deletion of the OSM receptor in osteogenic cells significantly ameliorated periodontitis-induced bone loss. Epigenomic data analyses identified the OSM-regulated RANKL enhancer region in osteogenic cells, and mice lacking this enhancer showed decreased periodontal bone loss while maintaining physiological bone metabolism. These findings shed light on the role of neutrophils in bone regulation during bacterial infection, highlighting the novel mechanism underlying osteoimmune crosstalk.

关键词： period neutrophil destruction

Dry eye rate and its relationship with disease stage in patients with primary hypertension:a cross-sectional study in Vietnam

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International Journal of Ophthalmology(English edition) 2024年第4期17卷 653-658页

作者： Tran Tat Thang Pham Hong Phuong Nguyen Sa huynh Nguyen Trung Kien NguyenDuy Toan Nguyen Thi Thu Ha Le Ha Khoa Nguyen Huu Dung Thai Doan Thang Le Viet Thang Nghe An Eye Hospital Nghe An 43000Vietnam Nghe An Friendship General Hospital Nghe An 43000Vietnam Military Hospital 103 Ha Noi 100000Vietnam Vietnam Military Medical University Ha Noi 100000Vietnam Hanoi Medical University Ha Noi 100000Vietnam Bạch Mai Hospital Ha Noi 100000Vietnam TTH Vinh General Hospital Nghe An 100000Vietnam

AIM:To determine the dry eye(DE)rate and its relationship with disease stage in patients with primary ***:A cross-sectional study included 432 patients with primary hypertension(with an equal number of patients in each group:144 in stage Ⅰ,Ⅱ,and Ⅲ hypertension)and 144 healthy subjects as a control *** Ocular Surface Disease Index(OSDI)and Schirmer Ⅰ test without anesthetics were conducted on all 576 *** with OSDI scores<13 and Schirmer Ⅰ values equal to or under 10 mm were diagnosed with ***:The ratio of DE in hypertension patients was higher than in the control group(41.7%versus 18.8%;P<0.001).The proportion of patients with DE increased gradually according to the hypertension stage:27.1% in stage Ⅰ,40.3% in stage Ⅱ,and 57.6% in stage Ⅲ,P<***,duration of hypertension,plasma urea,creatinine,and high-sensitivity C-reactive protein(CRP-hs)levels in hypertension patients with DE were higher than those without DE,P<*** age,a long duration of hypertension,diabetes mellitus,elevated plasma creatinine,and CRP-hs levels were independent factors associated with DE in primary hypertension patients,P<***:DE is a common disorder associated with advanced age,a long duration of hypertension,diabetes mellitus,elevated plasma CRP-hs,and creatinine levels in patients with primary hypertension.

关键词： primary hypertension dry eye stage of hypertension plasma creatinine

Effect of Freeze-Thaw and Urea in Solubility of GPC3-Csub Protein Expressed in Escherichia coli

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Journal of Biosciences and Medicines 2024年第4期12卷 288-297页

作者： Xuan-Truc Chu-Dao Kim-Tuyen huynh-Dam Dang-Thuc Ngo-Luong Quang-Luan Le Thanh-Thao Vo-Nguyen Department of Medical Biotechnology Biotechnology Center of Ho Chi Minh City Ho Chi Minh City Vietnam

Glypican-3 is a protein encoded by the Glypican-3 gene located on human X chromosome (Xq26), composed of two subunits, a 40 kDa N-terminal subunit, and a 30 kDa C-terminal subunit. Glypican-3 is a currently potential target molecule for liver cancer treatments because of its over-expression and growth effects on hepatocellular carcinoma (HCC). This study examined the expression and purification of a C-terminal subunit of Glypican-3 protein (GPC3-Csub) due to its application in both diagnosis and therapy for hepatocellular carcinoma. The gene encoding for GPC3-Csub was successfully cloned into plasmid pET28a fused with an affinity tag composed of six consecutive histidine residues (His-tag). Recombinant protein GPC3-Csub was expressed in Escherichia coli BL21 (DE3) in the condition of adding 3% ethanol with IPTG induction. GPC3-Csub was extracted using repeated freeze-thaw cycles with lysozyme, and inclusion bodies were solubilized by 8M Urea, SDS 10% in pH 12. His-tag fused GPC3-Csub proteins allowed it to be purified by affinity chromatography method using the Nickel-nitrilotriacetic acid (Ni-NTA) column. High expression of GPC3-Csub was confirmed by Coomassie staining and western-blot. GPC3-Csub could be isolated with a Ni-NTA column and have a purity of about 90%.

关键词： Glypican-3 Affinity Chromatography Inclusion Body Liver Cancer

Exploring the Interplay between Job Stress,Work Performance,and Attitudes toward Professional Psychological Help among Employees

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International Journal of Mental Health Promotion 2024年第7期26卷 531-545页

作者： Cong Minh Le Son Van huynh Ky Luu Quynh-Nhi Ngoc Ho Van Thi Le Nguyen Vinh-Long Tran-Chi Center for Mental Health Research and Support University of Social Sciences and HumanitiesVietnam National University Ho Chi Minh CityHo Chi Minh City700000Vietnam Faculty of Psychology Ho Chi Minh City University of EducationHo Chi Minh City700000Vietnam Faculty of Tourism College of BusinessUniversity of Economics Ho Chi Minh CityHo Chi Minh City700000Vietnam Scientific Management Department Dong A UniversityDa Nang500000Vietnam

Objective:This study explores the interplay between job stress,job-related factors,work performance,and attitudes toward seeking professional psychological help among Vietnamese ***:A total of 374 employees in Vietnam were surveyed using random sampling and an online questionnaire from November 07 to November 28,*** data and self-reported from three scales:The New Job Stress Scale(NJSS),Work Performance(WP),and Attitudes Toward Seeking Professional Psychological Help(ATSPPH_SF)were ***:Significant variations were found across several variables,including forms of work,operating hours,education levels,monthly income,numbers of daily working hours,and the presence of a psychological counseling department within the *** analysis has highlighted direct relationships between key latent *** who were more open to seeking professional help tended to report higher levels of job *** negative relationship was found between job stress and attitudes toward seeking professional ***,work effort was positively associated with work *** analyses revealed the influence of co-worker support on role expectation conflict and work effort,role expectation conflict and work-life balance,as well as interactions between role expectation conflict and attitudes needed toward seeking professional *** analyses showed that work effort mediated relationships between openness to seeking professional help,co-worker support,work-life balance,role expectation conflict,and work *** toward seeking professional help also mediated relationships between work-life balance,job stress,and work ***:The study highlights the complex dynamics surrounding job stress,job-related factors,work performance,and attitudes toward seeking professional psychological help among Vietnamese *** highlights the importance of addressing help-seeking barriers,promoting work engagement

关键词： Stress co-worker support work-life balance role expectation conﬂict professional psychological help-seeking

Structured NiFe catalysts derived from in-situ grown layered double hydroxides on ceramic monolith for CO_(2) methanation

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Green Energy & Environment 2020年第4期5卷 423-432页

作者： Huong Lan huynh Wakshum Mekonnen Tucho Zhixin Yu Department of Energy and Petroleum Engineering University of Stavanger4036 StavangerNorway Department of Mechanical and Structural Engineering and Material Science University of Stavanger4036 StavangerNorway

Monolithic catalysts for CO_(2) methanation have become an active research area for the industrial development of Power-to-Gas *** this study,we developed a facile and reproducible synthesis strategy for the preparation of structured NiFe catalysts on washcoated cordierite monoliths for CO_(2) *** NiFe catalysts were derived from in-situ grown layered double hydroxides(LDHs)via urea *** influence of different washcoat materials,i.e.,alumina and silica colloidal suspensions on the formation of LDHs layer was investigated,together with the impact of total metal *** LDHs were precipitated on the exterior surface of cordierite washcoated with alumina,while it was found to deposit further inside the channel wall of monolith washcoated with silica due to different intrinsic properties of the colloidal *** the other hand,the thickness of in-situ grown LDHs layers and the catalyst loading could be increased by high metal *** best monolithic catalyst(COR-AluCC-0.5M)was robust,having a thin and well-adhered catalytic layer on the cordierite *** a result,high methane yield was obtained from CO_(2) methanation at high flow rate on this structured NiFe *** monolithic catalysts appeared as promising structured catalysts for the development of industrial methanation reactor.

关键词： CO_(2)methanation Structured catalysts In-situ growth Layered double hydroxides Ceramic honeycomb monolith

AN ENSEMBLE MODEL OF ARIMA AND ANN WITH RESTRICTED BOLTZMANN MACHINE BASED ON DECOMPOSITION OF DISCRETE WAVELET TRANSFORM FOR TIME SERIES FORECASTING

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Journal of Systems Science and Systems Engineering 2018年第5期27卷 690-708页

作者： Warut Pannakkong Songsak Sriboonchitta Van-Nam huynh School of Manufacturing Systems and Mechanical Engineering Sirindhorn International Institute of TechnologyThammasat University Pathumthani 12121 Thailand Faculty of Economics Chiang Mai University Chiang Mai 52000 Thailand School of Knowledge Science Japan Advanced Institute of Science and Technology Nomi 923-1292 Japan

Time series forecasting research area mainly focuses on developing effective forecasting models toimprove prediction accuracy. An ensemble model composed of autoregressive integrated movingaverage （ARIMA）, artificial neural network （ANN）, restricted Boltzmann machines （RBM）, anddiscrete wavelet transform （DWT） is presented in this paper. In the proposed model, DWT firstdecomposes time series into approximation and detail. Then Khashei and Bijari＇s model, which is anensemble model of ARIMA and ANN, is applied to the approximation and detail to extract their bothlinear and nonlinear components and fit the relationship between the components as a function insteadof additive relationship. Furthermore, RBM is used to perform pre-training for generating initialweights and biases based on inputs feature for ANN. Finally, the forecasted approximation and detailare combined to obtain final forecasting. The forecasting capability of the proposed model is testedwith three well-known time series： sunspot, Canadian lynx, exchange rate time series. The predictionperformance is compared to the other six forecasting models. The results indicate that the proposedmodel gives the best performance in all three data sets and all three measures （i.e. MSE, MAE andMAPE）.

关键词： Time series forecasting autoregressive integrated moving average (ARIMA) artificial neural network (ANN) discrete wavelet transform (DWT) restricted Boltzmann machine (RBM)