检索结果-南通市图书馆

Expression Profile and Function Analysis of Long Non-coding RNAs in the Infection of Coxsackievirus B3

在线全文

学校读者我要写书评

暂无评论

Virologica Sinica 2019年第6期34卷 618-630页

作者： Lei Tong Ye Qiu Hui Wang Yunyue Qu Yuanbo zhao Lexun Lin Yan Wang Weizhen Xu wenran zhao Hongyan He Guangze zhao Mary HZhang Decheng Yang Xingyi Ge zhaohua Zhong Department of Microbiology Harbin Medical UniversityHarbin 150081China College of Biology Hunan UniversityChangsha 410012China Department of Cell Biology Harbin Medical UniversityHarbin 150081China Department of Pathology and Laboratory Medicine University of British ColumbiaVancouverBC V6Z 1Y6Canada

The roles of lnc RNAs in the infection of enteroviruses have been barely demonstrated. In this study, we used coxsackievirus B3(CVB3), a typical enterovirus, as a model to investigate the expression profiles and functional roles of lnc RNAs in enterovirus infection. We profiled lnc RNAs and m RNA expression in CVB3-infected He La cells by lnc RNA-m RNA integrated microarrays. As a result, 700 differentially expressed lnc RNAs(431 up-regulated and 269 down-regulated) and665 differentially expressed m RNAs(299 up-regulated and 366 down-regulated) were identified in CVB3 infection. Then we performed lnc RNA-m RNA integrated pathway analysis to identify potential functional impacts of the differentially expressed m RNAs, in which lnc RNA-m RNA correlation network was built. According to lnc RNA-m RNA correlation, we found that XLOC-001188, an lnc RNA down-regulated in CVB3 infection, was negatively correlated with NFAT5 m RNA,an anti-CVB3 gene reported previously. This interaction was supported by q PCR detection following si RNA-mediated knockdown of XLOC-001188, which showed an increase of NFAT5 m RNA and a reduction of CVB3 genomic RNA. In addition, we observed that four most significantly altered lnc RNAs, SNHG11, RP11-145 F16.2, RP11-1023 L17.1 and RP11-1021 N1.2 share several common correlated genes critical for CVB3 infection, such as BRE and IRF2 BP1. In all, our studies reveal the alteration of lnc RNA expression in CVB3 infection and its potential influence on CVB3 replication,providing useful information for future studies of enterovirus infection.

关键词： Coxsackievirus B3(CVB3) lncRNA-mRNA correlation network Long non-coding RNA(lncRNA) XLOC-001188 NFAT5

Expression of PHGPx in mammalian cells and its antiviral effect against coxsackievirus group B

在线全文

学校读者我要写书评

暂无评论

Progress in Natural Science:Materials International 2005年第7期15卷 665-668页

作者： zhao wenran, ZHONG Xuekuan and ZHOU Lingwang (Institute of Keshan Disease, Chinese Research Center for Endemic Disease Control, Harbin Medical University, Harbin 150086, China) Institute of Keshan Disease Chinese Research Center for Endemic Disease Control Harbin Medical University Harbin 150086 China

To express human phospholipid hydroperoxide glutathione peroxidase (PHGPx) in eukaryotic cells and to study its antiviral effect against Coxsackievirus B3m (CVB3m) in vitro, PHGPx cDNA was amplified from a human testis library using specific primers and cloned into expression vector pcDNA3. I/His. Expression of PHGPx was performed in COS-1 cells. The antiviral effect was studied by the treatment of HeLa cells with the recombinant PHGPx. Results showed that the activity of PHGPx expressed in COS-1 cells was 5-fold higher than that in control group, and it inhibited the cytopathic effect on HeLa cells caused by CVB3m. It can be concluded that recombinant PHGPx expressed in COS-1 cells has antiviral effect against CVB3m in vitro.

关键词： PHGPx gene expression coxsackievirus group B antiviral effect.

Stress Granule Formation is One of the Early Antiviral Mechanisms for Host Cells Against Coxsackievirus B Infection

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Virologica Sinica 2018年第4期33卷 314-322页

作者： Xia Zhai Shuo Wu Lexun Lin Tianying Wang Xiaoyan Zhong Yang Chen Weizhen Xu Lei Tong Yan Wang wenran zhao zhaohua Zhong Department of Microbiology Harbin Medical University Harbin 150081 China Department of Cell Biology Harbin Medical University Harbin 150081 China

Stress granules(SGs) are intracellular granules formed when cellular translation is blocked and have been reported to be involved in a variety of viral infections. Our previous studies revealed that SGs are involved in the coxsackievirus B(CVB)infection process, but the role of SGs in CVB infection has not been fully explored. In this study, we found that CVB type 3(CVB3) could induce SG formation in the early phase of infection. Results showed that levels of CVB3 RNA and protein were significantly inhibited during the early stage of CVB3 infection by the elevated formation of SGs, while viral RNA and protein synthesis were significantly promoted when SG formation was blocked. Our findings suggest that SG formation is one of the early antiviral mechanisms for host cells against CVB infection.

关键词： Coxsackievirus B (CVB) Stress granule (SG) Viral replication

Baicalein suppresses Coxsackievirus B3 replication by inhibiting caspase-1 and viral protease 2A

在线全文

学校读者我要写书评

暂无评论

Virologica Sinica 2024年第4期39卷 685-693页

作者： Yanyan Dong Enze Shao Siwei Li Ruiqi Wang Dan Wang Lixin Wang Hong Yang Yingxia He Tian Luan Yang Chen Yao Wang Lexun Lin Yan Wang zhaohua Zhong wenran zhao Department of Cell Biology Harbin Medical UniversityHarbin 150081China Department of Microbiology Harbin Medical UniversityHarbin 150081China

Myocarditis is an inflammatory disease of the cardiac muscle and one of the primary causes of dilated *** B coxsackievirus(CVB)is one of the leading causative pathogens of viral myocarditis,which primarily affects children and young *** to the lack of vaccines,the development of antiviral medicines is crucial to controlling CVB infection and the progression of *** this study,we investigated the antiviral effect of baicalein,a flavonoid extracted from Scutellaria *** results demonstrated that baicalein treatment significantly reduced cytopathic effect and increased cell viability in CVB3-infected *** addition,significant reductions in viral protein 3D,viral RNA,and viral particles were observed in CVB3-infected cells treated with *** found that baicalein exerted its inhibitory effect in the early stages of CVB3 *** also suppressed viral replication in the myocardium and effectively alleviated myocarditis induced by CVB3 *** study revealed that baicalein exerts its antiviral effect by inhibiting the activity of caspase-1 and viral protease *** together,our findings demonstrate that baicalein has antiviral activity against CVB3 infection and may serve as a potential therapeutic option for the myocarditis caused by enterovirus infection.

关键词： Baicalein Coxsackievirus B(CVB) Myocarditis 2A protease Caspase-1

Curcumin inhibits the replication of enterovirus 71 in vitro

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2014年第4期4卷 284-294页

作者： Ying Qin Lexun Lin Yang Chen Shuo Wu Xiaoning Si Heng Wu Xia Zhai Yan Wang Lei Tong Bo Pan Xiaoyan Zhong Tianying Wang wenran zhao zhaohua Zhong Department of Microbiology Harbin Medical UniversityHarbin 150086China Department of Cell Biology Harbin Medical UniversityHarbin 150086China

Human enterovirus 71(EV71)is the main causative pathogen of hand,foot,and mouth disease(HFMD)in *** epidemic of HFMD has been a public health problem in Asia-Pacific region for decades,and no vaccine and effective antiviral medicine are *** has been used as a traditional medicine for centuries to treat a diversity of disorders including viral *** this study,we demonstrated that curcumin showed potent antiviral effect again *** Vero cells infected with EV71,the addition of curcumin significantly suppressed the synthesis of viral RNA,the expression of viral protein,and the overall production of viral *** with the previous reports,curcumin reduced the production of ROS induced by viral ***,the antioxidant property of curcumin did not contribute to its antiviral activity,since N-acetyl-L-cysteine,the potent antioxidant failed to suppress viral *** study also showed that extracellular signal-regulated kinase(ERK)was activated by either viral infection or curcumin treatment,but the activated ERK did not interfere with the antiviral effect of curcumin,indicating ERK is not involved in the antiviral mechanism of *** the previous reports that curcumin inhibited protein degradation through ubiquitin–proteasome system(UPS),we found that curcumin had no impact on UPS in control ***,curcumin did reduce the activity of proteasomes which was increased by viral *** addition,the accumulation of the short-lived proteins,p53 and p21,was increased by the treatment of curcumin in EV71-infected *** further probed the antiviral mechanism of curcumin by examining the expression of GBF1 and PI4KB,both of which are required for the formation of viral replication *** found that curcumin significantly reduced the level of both ***,the decreased expression of either GBF1 or PI4KB by the application of siRNAs was sufficient to suppress viral *** also de

关键词： Curcumin Enterovirus 71 Viral replication GBF1 PI4KB Ubiquitin–proteasome system Apoptosis

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

内体和溶酶体来源的胞质空泡研究进展

国际免疫学杂志 2017年第2期40卷 78-83页

作者：赵子越赵文然哈尔滨医科大学细胞生物学教研室 150081

细胞应对细菌或病毒感染及来自环境的各种化学药物时常表现为在细胞质内形成大量空泡，其中有些空泡来源于内体和溶酶体，称为内体和溶酶体的空泡化。空泡的形成过程及分子机制复杂，有些细胞形成空泡时还伴随着细胞死亡，但空泡化与细... 详细信息

细胞应对细菌或病毒感染及来自环境的各种化学药物时常表现为在细胞质内形成大量空泡，其中有些空泡来源于内体和溶酶体，称为内体和溶酶体的空泡化。空泡的形成过程及分子机制复杂，有些细胞形成空泡时还伴随着细胞死亡，但空泡化与细胞死亡的关系仍不明确。因而对内体和溶酶体空泡化进行研究，以便更好地理解细胞对外源刺激的应激反应及其分子机制。

关键词：内体溶酶体胞质空泡

同方期刊数据库

运动神经元疾病中TDP43的翻译后修饰研究进展

在线全文

学校读者我要写书评

暂无评论

国际免疫学杂志 2019年第6期42卷 623-627页

作者：袁园赵文然哈尔滨医科大学基础医学院细胞生物学教研室 150081

肌萎缩性侧索硬化症(amyotrophic lateral sclerosis,ALS)是成人中最常见的运动神经元疾病.反式激活反应-DNA结合蛋白(TAR DNA-binding protein 43,TDP43)是ALS患者神经元内泛素化蛋白聚集物的主要成分.这些异常TDP43聚集物中存在多种修... 详细信息

肌萎缩性侧索硬化症(amyotrophic lateral sclerosis,ALS)是成人中最常见的运动神经元疾病.反式激活反应-DNA结合蛋白(TAR DNA-binding protein 43,TDP43)是ALS患者神经元内泛素化蛋白聚集物的主要成分.这些异常TDP43聚集物中存在多种修饰,如磷酸化、泛素化、乙酰化或SUMO化.这些翻译后的修饰如何以及在多大程度上促成病理TDP43聚集物的形成,有待进一步研究.

关键词：肌萎缩性侧索硬化症反式激活反应-DNA结合蛋白43 翻译后修饰

在线全文

学校读者我要写书评

暂无评论

热休克蛋白70与肠道病毒感染

国际免疫学杂志 2016年第2期39卷 184-188页

作者：王华鹏赵文然哈尔滨医科大学细胞生物学教研室 150081

热休克蛋白70（HSP70）是HSP70家族中的重要成员。HSP70的主要功能是作为分子伴侣参与蛋白质的折叠与修饰。在病毒感染时，HSP70参与机体的抗感染免疫，从而保护细胞。因而研究HSP70与肠道病毒的相互作用具有意义。

关键词：热休克蛋白70 肠道病毒 TOLL样受体细胞凋亡

The expression of PHGPx in mammalian cells and its antiviral effect against coxsackievirus group B

在线全文

学校读者我要写书评

暂无评论

The expression of PHGPx in mammalian cells and its antiviral...