The expression of PHGPx in mammalian cells and its antiviral effect against coxsackievirus group B
会议名称：《中国细胞生物学学会2005年学术大会、青年学术研讨会》
会议日期：2005年
学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 07[理学]
关 键 词：CVB The expression of PHGPx in mammalian cells and its antiviral effect against coxsackievirus group B
摘 要：Background: Keshan disease, an endemic cardiomyopathy, was found to be associated with selenium(Se) deficiency. Se supplementation to individuals in the endemic areas could completely prevent the development of Keshan disease. However, because of the seasonal and annual incidence of the disease, an infectious cofactor, in addition to Se deficiency, was postulated. Coxsackieviruses have been isolated from Keshan disease tissues and are known etiologic agents of viral-induced myocarditis. Se is an essential component of the antioxidant enzyme phospholipid hydroperoxide glutathione peroxidase (PHGPx). PHGPx is a unique antioxidant enzyme in that it is able to interact directly with peroxidized phospholipids and cholesterol and protect cells from oxidative damage. Since Keshan disease is associated with both virus infection and Se deficiency and Se supplementation could prevent the occurrence of the disease, we postulate that PHGPx, an important selenoenzyme, might play a role in antiviral process. In this work, we expressed PHGPx in mammalian cells and detected its protective effect on HeLa cells against Coxsackievirus group B3m(CVB3m) infection. Methods: PHGPx cDNA was amplified from a human testis library using specific primers and cloned into expression vector pcDNA3.1/His. Expression of PHGPx was performed in COS-1 cells. The antiviral effect was studied by the treatment of HeLa cells with the recombinant PHGPx. Results and conclusion: The activity of PHGPx expressed in COS-1 cells was 5-fold higher than that in control group, and it inhibited the cytopathic effect on HeLa cells caused by CVB3m. It can be concluded that recombinant PHGPx expressed in COS-1 cells has antiviral effect against CVB3m in vitro.