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High dietary lipid level promotes low salinity adaptation in the marine euryhaline crab(Scylla paramamosain)

Animal Nutrition 2023年第1期12卷 297-307页

作者： Jiaxiang luo Chen Ren Tingting Zhu Chen Guo Shichao Xie Yingying Zhang Zheng Yang wenli Zhao Xiangsheng Zhang Jingjing Lu Lefei Jiao Qicun Zhou Douglas R.Tocher Min Jin Laboratory of Fish and Shellfish Nutrition School of Marine SciencesNingbo UniversityNingbo 315211China Guangdong Provincial Key Laboratory of Marine Biotechnology Institute of Marine SciencesShantou UniversityShantou 515063China

The physiological processes involved in adaptation to osmotic pressure in euryhaline crustaceans are highly energy demanding,but the effects of dietary lipids(fat)on low salinity adaptations have not been well *** the present study,a total of 120 mud crabs(Scylla paramamosain,BW=17.87±1.49 g)were fed control and high-fat(HF)diets,at both medium salinity(23‰)and low salinity(4‰)for 6 wk,and each treatment had 3 replicates with each replicate containing 10 *** results indicated that a HF diet significantly mitigated the reduction in survival rate,percent weight gain and feed efficiency induced by low salinity(P<0.05).Low salinity lowered lipogenesis and activated lipolysis resulting in lipid depletion in the hepatopancreas of mud crabs(P<0.05).Thus,HF diets enhanced the process of lipolysis to supply more *** the gills,low salinity and the HF diet increased the levels of mitochondrial biogenesis markers,the activity of mitochondrial complexes,and the expression levels of genes related to energy metabolism(P<0.05).Consequently,the positive effects of the HF diet on energy metabolism in mud crabs at low salinity promoted osmotic pressure ***,significantly higher haemolymph osmotic pressure and inorganic ion content,as well as higher osmotic pressure regulatory enzyme activity in gills,and gene and protein expression levels of NaK-ATPase were observed in crabs fed the HF diet at low salinity(P<0.05).In summary,high dietary lipid levels improved energy provision to facilitate mitochondrial biogenesis,which increased ATP provision for osmotic pressure regulation of mud *** study also illustrates the importance of dietary lipid nutrition supplementation for low salinity adaptations in mud crabs.

关键词： Osmotic pressure regulation Mitochondrial energy metabolism Lipid metabolism

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UPLC-MS/MS同时测定京制咳嗽痰喘丸中马兜铃酸Ⅰ、Ⅱ的含量

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亚太传统医药 2024年第4期20卷 16-20页

作者：李靖云孙辉丁野李文莉刘静戴忠马双成罗疆南湖南省药品检验检测研究院湖南长沙410001 湖南省药品质量评价工程技术研究中心湖南长沙410001 中国食品药品检定研究院北京100050

目的:建立超高效液相色谱-质谱法(UPLC-MS/MS)同时测定京制咳嗽痰喘丸中马兜铃酸Ⅰ、Ⅱ的含量方法。方法:采用Agilent Poroshell SB-C_(18)色谱柱(100 mm×2.1 mm,2.7μm),以甲醇(A)-5 mmol/L甲酸铵(含0.1%甲酸),(B)为流动相进行梯度洗... 详细信息

目的:建立超高效液相色谱-质谱法(UPLC-MS/MS)同时测定京制咳嗽痰喘丸中马兜铃酸Ⅰ、Ⅱ的含量方法。方法:采用Agilent Poroshell SB-C_(18)色谱柱(100 mm×2.1 mm,2.7μm),以甲醇(A)-5 mmol/L甲酸铵(含0.1%甲酸),(B)为流动相进行梯度洗脱,流速为0.3 mL/min,柱温为30℃,进样量为1μL。质谱采用电喷雾离子源(ESI),正离子模式下多反应监测(MRM),标准曲线法测定。结果:马兜铃酸I和马兜铃酸Ⅱ分别在1.052 4~210.48 pg(R^(2)=0.998 1,n=6)、1.002~100.2 pg(R^(2)=0.999 9,n=6)范围内线性关系良好,平均加样回收率分别为96.09%(RSD=2.08%)和88.05%(RSD=2.64%)。结论:该方法简便、灵敏、专属性好,可用于京制咳嗽痰喘丸马兜铃酸Ⅰ、Ⅱ的含量测定。

关键词：京制咳嗽痰喘丸马兜铃酸Ⅰ 马兜铃酸Ⅱ 超高效液相色谱-质谱联用法

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First application of 3D design custom-made uncemented prosthetic stem for distal femoral cemented megaprosthesis revision

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Precision Clinical Medicine 2018年第2期1卷 88-96页

作者： Li Min Kai Yao Minxun Lu Yong Zhou Jie Wang Fan Tang wenli Zhang Yi luo Hong Duan Chongqi Tu Department of Orthopedics West China HospitalSichuan UniversityGuoxue Xiang#37ChengduSichuan 610041People’s Republic of China

Objectives:3D design,which is widely used in orthopedics,can be applied for precise distal femoral megaprosthetic *** research aimed to present and evaluate the design,perioperative management,and midterm clinical outcomes of a 3D design custom-made uncemented prosthetic ***:Between January 2014 and January 2016,seven patients received 3D design custom-made uncemented prosthetic stem revision at our *** records and radiographs were evaluated ***:There were no hardware-related complications during the follow-up(average 24.3 months;range 24–48 months).The average Musculoskeletal Tumor Society(MSTS)score at the last follow-up after revision(27.7 points,range 25–28 points)was significantly higher than that before(16.0 points,range 13–18 points).In addition,the range of motion(ROM)of the affected knee,and the scores of pain,function,emotional acceptance,support,walking and gait all improved *** antecurvature radian of the revision stem averaged at 3.6°.Of the seven patients,three received femoral stem revision and four received revision of the femoral stem and the femoral component;three of them used longer prostheses than the *** were no significant differences in function between these two groups at the last follow-up after ***:The 3D design custom-made prosthesis is a typical precision medicine technology in oncologic *** by its individually and precisely designed uncemented stem,it offers an alternative option for distal femoral cemented prosthesis *** the 3D design itself,the perioperative management,especially the techniques for stem implantation,and long-term follow-up are also crucial.

关键词： distal femur bone tumor 3D design prosthetic stem revision precision medicine

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Population transcriptomics reveals a potentially positive role of expression diversity in adaptation

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Journal of Integrative Plant Biology 2015年第3期57卷 284-299页

作者： Qin Xu Shilai Xing Caiyun Zhu Wei Liu Yangyang Fan Qian Wang Zhihong Song Wenhui Yang Fan luo Fei Shang Lifang Kang wenli Chen Juan Yan Jianqiang Li Tao Sang State Key Laboratory of Systematic and Evolutionary Botany Institute of Botanythe Chinese Academy of Sciences University of Chinese Academy of Sciences Key Laboratory of Plant Resources and Beijing Botanical Garden Institute of Botanythe Chinese Academy of Sciences Key Laboratory of Plant Germplasm Enhancement and Speciality Agriculture Wuhan Botanical Gardenthe Chinese Academy of Sciences

While it is widely accepted that genetic diversity determines the potential of adaptation,the role that gene expression variation plays in adaptation remains poorly *** we show that gene expression diversity could have played a positive role in the adaptation of Miscanthus ***-seq was conducted for 80 individuals of the species,with half planted in the energy crop domestication site and the other half planted in the control site near native habitats.A leaf reference transcriptome consisting of 18,503 high-quality transcripts was obtained using a pipeline developed for de novo assembling with population RNA-seq *** population structure and genetic diversity of *** were estimated based on 30,609 genic single nucleotide *** expression（Ep） and expression diversity（Ed）were defined to measure the average level and the magnitude of variation of a gene expression in the population,*** was found that expression diversity increased while genetic Resediversity decreased after the species was transplanted from the native habitats to the harsh domestication site,especially for genes involved in abiotic stress resistance,histone methylation,and biomass synthesis under water *** increased expression diversity could have enriched phenotypic variation directly subject to selections in the new environment.

关键词： Adaptation artificial selection de novo assembling expression variation genetic diversity Miscanthus lutarioriparius population transcriptome

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我国部分地区40~89岁中老年人尿镉水平与外周血白细胞分类计数的关联研究

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中华预防医学杂志 2024年第6期58卷 839-846页

作者：罗宇菲魏源王晓晨张翼张文丽吴兵杨正雄董晓杰郝瑞婷陆一夫符小霜朱子月朱英吕跃斌徐东群施小明安徽医科大学公共卫生学院合肥230032 中国疾病预防控制中心环境与人群健康重点实验室环境与健康相关产品安全所北京100021 吉林大学公共卫生学院长春130012 南京医科大学公共卫生学院南京211166

目的探讨我国部分地区40~89岁中老年人尿镉水平与外周血白细胞分类计数的关联。方法本研究基于2019—2020年“典型地区农用地土壤质量对人群健康影响调查”项目,采用多阶段分层随机抽样方法,共纳入5600名40~89岁的中老年人。收集研究对... 详细信息

目的探讨我国部分地区40~89岁中老年人尿镉水平与外周血白细胞分类计数的关联。方法本研究基于2019—2020年“典型地区农用地土壤质量对人群健康影响调查”项目,采用多阶段分层随机抽样方法,共纳入5600名40~89岁的中老年人。收集研究对象的基线特征,进行体格检查;采集随机中段尿以检测尿镉和尿肌酐;采集空腹静脉血以检测白细胞计数、中性粒细胞计数、淋巴细胞计数、单核细胞计数和嗜酸性粒细胞计数。采用线性混合效应模型分析尿镉水平与外周血白细胞分类计数的关联,并采用限制性立方样条函数探索二者之间的剂量-反应关系。结果研究对象的年龄为(63.17±12.02)岁;男性为2851名(50.91%);尿肌酐校正后尿镉水平的M(Q1,Q3)为2.69(1.52,4.69)μg/g·肌酐。校正相关混杂因素后,线性混合效应模型分析结果显示,尿肌酐校正后尿镉水平每增加1个单位,白细胞计数和淋巴细胞计数的变化百分比[%(95%CI)]分别为-1.70%(-2.61%,-0.79%)和-1.57%(-2.86%,-0.26%)。限制性立方样条函数分析结果显示,尿肌酐校正后尿镉水平与白细胞计数、淋巴细胞计数分别呈负向线性关系(均P非线性>0.05)。结论我国部分地区40~89岁中老年人尿肌酐校正后尿镉水平与白细胞计数、淋巴细胞计数呈负向关联。

关键词：镉白细胞计数淋巴细胞计数中老年人

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Ultrasensitive flexible broadband photodetectors achieving pA scale dark current

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npj Flexible Electronics 2017年第1期1卷 39-46页

作者： Xiao luo Feiyu Zhao Lili Du wenli Lv Kun Xu Yingquan Peng Ying Wang Feiping Lu Institute of Microelectronics College of Optical and Electronic TechnologyChina Jiliang UniversityXueyuan Street 258#Hangzhou 310018China Institute of Microelectronics School of Physical Science and TechnologyLanzhou UniversitySouth Tianshui Road 222#Lanzhou 730000China College of Information Engineering China Jiliang UniversityXueyuan Street 258#Hangzhou 310018China Department of Physics Tianshui Normal UniversityTianshui 741001China

Organolead halide perovskite is a newly emerging low-cost,solution-processable material with a broadband absorption from the ultraviolet(UV)to visible(Vis)region,which has attracted a great deal of interest in high-performance optoelectronic ***,some practicable applications need a cover of UV–Vis–NIR region for photoelectric conversion,a task that remains a significant challenge for further extending the absorption toward the near-infrared radiation(NIR)***,to the best of our knowledge,we prove for the first time an ultrasensitive flexible broadband photodetector based on porous organolead perovskitephthalocyanine heterostructure,which combines the synergetic properties of high UV–Vis absorbance of perovskite with enhanced NIR absorption for triclinic lead *** photosensitivity of the as-prepared devices reaches up to 10^(4) at a low intensity of 10mWcm^(−2),which is among the largest values reported for broadband ***,performed at room temperature,the device achieves a pA scale dark current along with an ultrafast response speed of less than 0.6 ms for as-adopted full *** results provide an easy and promising route to develop low-cost,flexible and highly sensitive UV–Vis–NIR photodetectors.

关键词： optoelectronic absorption perovskite

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Individualized red-cell transfusion strategy for non-cardiac surgery in adults:a randomized controlled trial

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Chinese Medical Journal 2023年第23期136卷 2857-2866页

作者： Ren Liao Jin Liu Wei Zhang Hong Zheng Zhaoqiong Zhu Haorui Sun Zhangsheng Yu Huiqun Jia Yanyuan Sun Li Qin wenli Yu Zhen luo Yanqing Chen Kexian Zhang Lulu Ma Hui Yang Hong Wu Limin Liu Fang Yuan Hongwei Xu Jianwen Zhang Lei Zhang Dexing Liu Han Huang Department of Anesthesiology Research Unit for Perioperative Stress Assessment and Clinical DecisionChinese Academy of Medical Sciences(2018RU012)West China Hospital of Sichuan UniversityChengduSichuan 610041China Department of Anesthesiology The First Affiliated Hospital of Zhengzhou UniversityZhengzhouHenan 450052China Department of Anesthesiology The First Affiliated Hospital of Xinjiang Medical UniversityUrumqiXinjiang 830054China Department of Anesthesiology The Affiliated Hospital of Zunyi Medical CollegeZunyiGuizhou 563003China Department of Anesthesiology Beijing Friendship Hospital of Capital Medical UniversityBeijing 100069China Department of Bioinformatics and Biostatistics School of Life Science and BiotechnologyShanghai Jiao Tong UniversityShanghai 200240China Department of Anesthesiology The Fourth Hospital of Hebei Medical UniversityShijiangzhuangHebei 050019China Department of Anesthesiology Xijing Hospital of the Fourth Military Medical UniversityXi’anShaanxi 710032China Department of Transfusion West China Hospital of Sichuan UniversityChengduSichuan 610041China Department of Anesthesiology Tianjin First Center HospitalTianjin 300192China Department of Anesthesiology Fujian Provincial HospitalFuzhouFujian 350001China Department of Anesthesiology Sichuan Cancer Hospital&InstituteChengduSichuan 610041China Department of Anesthesiology Peking Union Medical College HospitalBeijing 100032China Department of Anesthesiology Tongji Hospital of Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei 430030China Department of Hepato-Bilio-Pancreatic Surgery West China Hospital of Sichuan UniversityChengduSichuan 610041China Department of Orthopedic Surgery West China Hospital of Sichuan UniversityChengduSichuan 610041China Department of Anesthesiology General Hospital Affiliated to Tianjin Medical UniversityTianjin 300052China Department of Anesthesiology and Translational Neuroscience Center West China Second University Hospital of S

Background:Red-cell transfusion is critical for surgery during the peri-operative period;however,the transfusion threshold remains controversial mainly owing to the diversity among *** patient’s medical status should be evaluated before making a transfusion ***,we developed an individualized transfusion strategy using the West-China-Liu’s Score based on the physiology of oxygen delivery/consumption balance and designed an open-label,multicenter,randomized clinical trial to verify whether it reduced red cell requirement as compared with that associated with restrictive and liberal strategies safely and effectively,providing valid evidence for peri-operative ***:Patients aged>14 years undergoing elective non-cardiac surgery with estimated blood loss>1000 mL or 20%blood volume and hemoglobin concentration<10 g/dL were randomly assigned to an individualized strategy,a restrictive strategy following China’s guideline or a liberal strategy with a transfusion threshold of hemoglobin concentration<9.5 g/*** evaluated two primary outcomes:the proportion of patients who received red blood cells(superiority test)and a composite of in-hospital complications and all-cause mortality by day 30(non-inferiority test).Results:We enrolled 1182 patients:379,419,and 384 received individualized,restrictive,and liberal strategies,*** 30.6%(116/379)of patients in the individualized strategy received a red-cell transfusion,less than 62.5%(262/419)in the restrictive strategy(absolute risk difference,31.92%;97.5%confidence interval[CI]:24.42-39.42%;odds ratio,3.78%;97.5%CI:2.70-5.30%;P<0.001),and 89.8%(345/384)in the liberal strategy(absolute risk difference,59.24%;97.5%CI:52.91-65.57%;odds ratio,20.06;97.5%CI:12.74-31.57;P<0.001).No statistically significant differences were found in the composite of in-hospital complications and mortality by day 30 among the three ***:The individualized red-cell transfusion stra

关键词： Individualized transfusion strategy West-China-Liu’s Score Red cells Blood volume Hemoglobins Postoperative care

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A novel peptide specifically binding to VEGF receptor suppresses angiogenesis in vitro and in vivo

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Signal Transduction and Targeted Therapy 2017年第1期2卷 222-228页

作者： Yuan Zhang Bifang He Kun Liu Lin Ning Delun luo Kai Xu wenli Zhu Zhigang Wu Jian Huang Xun Xu Department of Ophthalmology Shanghai General HospitalShanghai Jiaotong University School of MedicineShanghaiChina Key Laboratory for NeuroInformation of Ministry of Education School of Life Science and TechnologyUniversity of Electronic Science and Technology of ChinaChengduChina Chengdu Nuoen Biotechnologies LTDChengduChina Center for Informational Biology University of Electronic Science and Technology of ChinaChengduChina

Vascular endothelial growth factor(VEGF),one of the most important angiogenic factors,plays an essential role in both physiological and pathological angiogenesis through binding to VEGF receptors(VEGFRs).Here we report a novel peptide designated HRHTKQRHTALH(peptide HRH),which was isolated from the Ph.D.-12 phage display library using VEGFR-Fc fusion protein as the *** peptide was found to dose-dependently inhibit the proliferation of human umbilical vein endothelial cells stimulated by *** anti-angiogenesis effect of the HRH peptide was further confirmed in vivo using the chick chorioallantoic membrane assay,which was also ***,peptide HRH was proved to inhibit corneal neovascularization in an alkali-burnt rat corneal model and a suture-induced rat corneal *** together,these findings suggest that the HRH peptide can inhibit angiogenesis both in vitro and in ***,the HRHTKQRHTALH peptide might be a promising lead peptide for the development of potential angiogenic inhibitors.

关键词： angiogenesis vivo VEGFR

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超声高清微血流成像技术在鉴别乳腺良恶性结节中的应用价值

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中华医学超声杂志（电子版） 2023年第12期20卷 1242-1247页

作者：罗润兰蒋文莉张艳罗渝昆中国人民解放军总医院第一医学中心超声诊断科北京100853

目的评估超声高清微血流成像(HD-MFI)技术在鉴别乳腺结节良恶性中的应用价值。方法这是一项前瞻性研究。选取2022年3月至2022年6月于中国人民解放军总医院第一医学中心行乳腺常规超声检查提示BI-RADS 3~5类的结节,所有结节均最终获得病... 详细信息

目的评估超声高清微血流成像(HD-MFI)技术在鉴别乳腺结节良恶性中的应用价值。方法这是一项前瞻性研究。选取2022年3月至2022年6月于中国人民解放军总医院第一医学中心行乳腺常规超声检查提示BI-RADS 3~5类的结节,所有结节均最终获得病理结果。所有结节均分别行彩色多普勒血流成像(CDFI)及HD-MFI扫查,以获得结节的血流情况,并通过HD-MFI获得结节的微血管模式(无血管、线状、树枝状、毛根样及蟹足样)。比较CDFI及HD-MFI对良性结节与恶性结节的血流检出情况及良性结节与恶性结节HD-MFI微血管模式的差异,通过ROC曲线对比分析CDFI与HD-MFI对乳腺结节良恶性的诊断效能。结果最终共纳入114例患者的119个乳腺结节,其中71个为良性结节,48个为恶性结节。HD-MFI较CDFI可以更多地检测出乳腺结节的3级血流信号(72.3%vs 20.1%)。在HD-MFI中,良恶性结节均多为2、3级血流信号,但恶性结节的3级血流信号比例显著高于良性结节(85.4%vs 63.4%,P<0.05)。HD-MFI显示良性结节多为无血管、线状或树枝样血管模式,恶性结节多为毛根样或蟹足样模式,微血管模式差异有统计学意义(P<0.05)。HD-MFI对乳腺结节良恶性的诊断特异度(91.5%vs 81.7%)、准确度(86.6%vs 82.4%)、阳性预测值(86.4%vs 75.5%)及曲线下面积[0.854(95%CI:0.777~0.912)vs 0.825(95%CI:0.745~0.889)]均高于CDFI。结论与CDFI相比,HD-MFI可以更敏感地检出乳腺结节的血流信号,其描绘的血管模式可以为乳腺结节良恶性的鉴别诊断提供更多的血流信息。

关键词：乳腺肿瘤微血管微血流成像超声检查彩色多普勒血流成像

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先锋蛋白肽对茶饼病的防效及茶叶品质的影响

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贵州农业科学 2024年第9期52卷 59-66页

作者：冯月玲杨文利罗涯鎔郑周洲朱秀英张三中周增太宗志伦陈兴龙吴元竞张蓉刘辉宋莉贵州大学生命科学学院/农业生物工程研究院山地植物资源保护与保护种质创新教育部重点实验室贵州贵阳550025 贵州省生物技术研究所/贵州省农业生物技术重点实验室贵州贵阳550006 贵阳康养职业大学贵州贵阳550081 乌当区农业农村局粮油茶产业站贵州贵阳550018 贵州师范学院贵州贵阳550018 贵州乾之象生物科技有限责任公司贵州贵阳550001 襄阳维恩生物科技有限公司湖北襄阳441200 贵州天贵茶业有限公司贵州贵阳550021 贵州发来地农业科技有限公司贵州龙里551202 贵州省贵阳市乌当区新堡布依族乡农业综合服务中心贵州贵阳550021

【目的】探明茶园喷施先锋蛋白肽对茶饼病的防控效果及茶叶品质的影响,为其在茶园病害绿色防控及茶叶提质增效上应用提供依据。【方法】采用常规喷雾法,以喷施清水(CK1)和药剂〔CK2,25%吡唑醚菌酯水剂(140.63 g a.i./hm^(2))〕为对照,... 详细信息

【目的】探明茶园喷施先锋蛋白肽对茶饼病的防控效果及茶叶品质的影响,为其在茶园病害绿色防控及茶叶提质增效上应用提供依据。【方法】采用常规喷雾法,以喷施清水(CK1)和药剂〔CK2,25%吡唑醚菌酯水剂(140.63 g a.i./hm^(2))〕为对照,研究先锋蛋白肽(600 g a.i./hm^(2))不同施用次数(茶叶采摘前喷施1次、2次和3次)对茶饼病的田间防控潜力和茶叶品质的影响。【结果】茶叶喷施先锋蛋白肽1次、2次和3次对茶饼病的相对防效分别为73.91%、69.57%和21.74%,喷施2次和3次的相对防效显著低于CK2(77.50%),与CK1相比,CK2对茶叶的增产效果仅为1.23%,而施用先锋蛋白肽1次、2次和3次的茶叶增产效果分别为4.01%、26.46%和9.32%,相应的发芽密度(1587.71个/m^(2))和百芽重(21.04 g)均以施药2次的最高。施药次数不同对茶叶中咖啡碱含量影响不显著,但对茶多酚和游离氨基酸含量影响较大。鲜味、甜味氨基酸天冬氨酸(2.68 mg/g)、甘氨酸(0.23 mg/g)和丙氨酸(0.08 mg/g)含量在施药2次的茶叶中最高,在施药3次的茶叶中则以苦味氨基酸缬氨酸(0.15 mg/g)、亮氨酸(0.37 mg/g)、酪氨酸(0.08 mg/g)和组氨酸(0.28 mg/g)含量最高;酚氨比施药2次(3.56)的最低,施药3次(4.22)的最高;施药1次的茶叶表没食子儿茶素(1.03%)、表没食子儿茶素没食子酸酯(2.65%)、表儿茶素没食子酸酯(0.71%)含量显著低于施药2次(1.22%、2.96%和0.81%),表儿茶素(0.73%)含量显著上升;苯丙氨酸(2.48 mg/g)、胱氨酸(6.57 mg/g)、蛋氨酸(6.63 mg/g)和脯氨酸(0.29 mg/g)等抗逆相关氨基酸含量以施药1次的最高。【结论】先锋蛋白肽施药2次既可高效防控茶饼病,亦可提高茶叶产质量,达到较好的防病、增产和提质效果。

关键词：先锋蛋白肽生物源药剂茶饼病施药次数病情指数田间防效茶产量

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