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Construction of millimeter-scale vascularized engineered myocardial tissue using a mixed gel

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Regenerative Biomaterials 2024年第3期3卷 56-67页

作者： Ming Ke wenhui xu Yansha Hao Feiyang Zheng Guanyuan Yang Yonghong Fan Fangfang Wang Zhiqiang Nie Chuhong Zhu Department of Anatomy Third Military Medical UniversityChongqing 400038China State Key Laboratory of Trauma Burn and Combined InjuryChongqing 400038China Department of Plastic and Aesthetic Surgery Southwest HospitalThird Military Medical UniversityChongqing 400038China Engineering Research Center of Tissue and Organ Regeneration and Manufacturing Ministry of EducationChongqing 400038China

Engineering myocardium has shown great clinal potential for repairing permanent myocardial ***,the lack of perfusing blood vessels and difficulties in preparing a thick-engineered myocardium result in its limited clinical *** prepared a mixed gel containing fibrin(5 mg/ml)and collagen I(0.2 mg/ml)and verified that human umbilical vein endothelial cells(HUVECs)and human-induced pluripotent stem cell-derived cardiomyocytes(hiPSC-CMs)could fom microvascular lumens and myocardial cell clusters by harnessing the low-hardness and hyperelastic characteristics of ***-CMs and HUVECs in the mixed gel formed self-organized cell clusters,which were then cultured in different media using a three-phase *** successfully constructed vascularized engineered myocardial tissue had a spherical structure and final diameter of *** tissue exhibited autonomous beats that occured at a frequency similar to a normal human heart *** internal microvascular lumen could be maintained for 6 weeks and showed good results during preliminary surface re-vascularization in vitro and vascular remodeling in *** summary,we propose a simple method for constructing vascularized engineered myocardial tissue,through phased cultivation that does not rely on high-end manufacturing equipment and cutting-edge preparation *** constructed tissue has potential value for clinical use after preliminary evaluation.

关键词： engineered myocardium vascularized hiPSC-CM HUVEC fibrin

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红外图像量化影响目标检测性能实验研究

实验室研究与探索 2024年第5期43卷 15-20页

作者：徐文辉钟胜邹旭何顶新华中科技大学人工智能与自动化学院武汉430074 华中科技大学多谱信息智能处理技术全国重点实验室武汉430074

为了研究不同红外图像量化方法对目标检测网络性能影响的差异,将红外图像量化对深度学习目标检测网络性能影响的研究和分析设计成教学实验。实验内容涉及图像处理、模式识别、计算机视觉等多个专业课程。实验过程包括红外图像量化、网... 详细信息

为了研究不同红外图像量化方法对目标检测网络性能影响的差异,将红外图像量化对深度学习目标检测网络性能影响的研究和分析设计成教学实验。实验内容涉及图像处理、模式识别、计算机视觉等多个专业课程。实验过程包括红外图像量化、网络模型训练、测试分析等多个环节,贯穿基于深度学习的高层视觉任务开发全流程。该实验紧跟学科前沿,促进学生科研能力和综合素质的培养。

关键词：红外图像量化目标检测人工智能教研协同

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生物标志物在结核病诊断中的研究进展

中国防痨杂志 2024年第6期46卷 713-721页

作者：徐文辉张艳秋石洁孙定勇郑州大学公共卫生学院流行病学系郑州450001 河南省疾病预防控制中心结核病预防控制所郑州450016

结核病是全球性的公共卫生问题,结核分枝杆菌生物标志物可以用于结核病的早期诊断和治疗监测。但现有结核病诊断方法存在不足,亟需寻找新的易获取的快速诊断结核病的生物标志物,以探索快速诊断结核病的新方法。本综述介绍了来源于血液... 详细信息

结核病是全球性的公共卫生问题,结核分枝杆菌生物标志物可以用于结核病的早期诊断和治疗监测。但现有结核病诊断方法存在不足,亟需寻找新的易获取的快速诊断结核病的生物标志物,以探索快速诊断结核病的新方法。本综述介绍了来源于血液、尿液、唾液及其他样本的结核病生物标志物最新研究进展,为开发新型结核病诊断方法提供线索。

关键词：结核生物因子生物学标记诊断综述文献(主题)

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Bending of a Saturated Ferromagnetoelastic Plate Under a Local Mechanical Load

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Acta Mechanica Solida Sinica 2023年第6期36卷 794-801页

作者： Jiashi Yang Xiaoshan Cao wenhui xu Department of Mechanical and Materials Engineering University of Nebraska-LincolnLincolnNE 68588-0526USA School of Civil Engineering Xi’an University of TechnologyXi’an 710048China

We study the bending of a magnetically saturated ferromagnetoelastic *** plate is rectangular and simply-supported along its *** is under a local distribution of normal mechanical load on its top surface,simulating a mechanical probe or manipulation of the magnetization *** three-dimensional equations of saturated ferromagnetoelasticity for small fields superposed on finite biasing fields due to spontaneous magnetization are *** plate is effectively piezomagnetic under the biasing fields.A trigonometric series solution is *** perturbation of the magnetization field by the mechanical load is calculated and *** is found that the magnetization is sensitive to the mechanical load,particularly near the loading *** perturbation of the magnetization is found to be associated with the transverse shear stresses in bending.

关键词： Magnetoelastic Piezomagnetic Ferromagnetic Spin wave Phonon-magnon coupling

Making polymer fibers strong and tough simultaneously

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Science China Materials 2020年第3期63卷 481-482页

作者： wenhui xu Yichun Ding School of Pharmacy Jiangxi University of Traditional Chinese MedicineNanchang 330004China Fujian Institute of Research on the Structure of Matter Chinese Academy of SciencesFuzhou 350002China

It has long been a great challenge to prepare materials with integrated merits of high strength,high modulus,high toughness,and light weight for structural material applications(e.g.,aircrafts,automobiles).High strength representing a good resistance to deformation usually requires the material have a rigid/hard molecular structure,whereas high toughness representing a good resistance to fracture typically originates from a soft/flexible molecular ***,the trade-off between the rigid and flexible components in a molecule usually leads to an intrinsic conflict between high strength and high toughness[1].It is more intuitively in the typical stressstrain curves,where the integrated area of a tensile curve can be calculated for the ***,the attainment of high toughness needs not only high strength but also high tensile strain.

关键词： toughness strength representing

不同提取方法对黄精多糖理化特性和生物活性的影响

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现代食品科技 2024年第3期40卷 142-152页

作者：覃引徐文慧吴凯孟攀蔡菊林长松贵州理工学院食品药品制造工程学院贵州贵阳550003 贵州大学生命科学学院贵州贵阳550025 贵州大学酿酒与食品工程学院贵州贵阳550025

为比较不同提取方法对黄精多糖理化特性和生物活性的影响,该文以黄精根茎为原料,分别用水提法(Water Extraction,WE)、酶解法(Enzyme Extraction,EE)、超声辅助法(Ultrasonic-assisted Extraction,UE)提取黄精多糖(Polygonatum sibiricum Polysaccharides,PP),比较了不同提取方法对黄精多糖得率、化学组成、理化特性(持油力、醇溶性、吸湿性)、生物活性(抗氧化活性、α-葡萄糖甘酶抑制活性、α-淀粉酶抑制活性)、单糖组成及分子量大小的影响。结果表明:酶解法较其他两种提取方法相比,具有较高的得率(8.11%)、总糖含量(52.79%)、持油力(5.67 g/g)、吸湿率(25.28%)和重均分子量(5.343×10^(5) g/mol)。三种提取方法得到的多糖对2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS^(+))的半数抑制质量浓度IC_(50)(mg/mL)分别为0.41、0.23、0.25;对1,1-二苯基-2-三硝基苯肼(DPPH)的半数抑制质量浓度IC50(mg/mL)分别为0.19、0.16、0.18;对α-葡萄糖甘酶的半数抑制质量浓度IC50(mg/mL)分别为2.14、1.98、2.06;对α-淀粉酶的半数抑制质量浓度IC50(mg/mL)分别为0.62、0.41、0.58。三种多糖均为酸性多糖,单糖组成以葡萄糖为主。通过红外光谱检测到3种多糖均具有多糖特征官能团,电镜扫描结果呈现不同的结构特征。综上,不同提取方法对黄精多糖的理化特性、生物活性、单糖组成和微观结构的影响有差异,酶解法是一种可以提高多糖理化性质和生物活性的有效方法,黄精多糖具有一定生物活性和可开发利用价值。

关键词：黄精多糖提取方法抗氧化活性 α-葡萄糖甘酶抑制活性、α-淀粉酶抑制活性理化性质

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GREEDY NON-DOMINATED SORTING IN GENETIC ALGORITHM-ⅡFOR VEHICLE ROUTING PROBLEM IN DISTRIBUTION

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Chinese Journal of Mechanical Engineering 2008年第6期21卷 18-24页

作者： WEI Tian FAN wenhui xu Huayu National CIMS Engineering Research Center Tsinghua University Beijing 100084 China

Vehicle routing problem in distribution （VRPD） is a widely used type of vehicle routing problem （VRP）, which has been proved as NP-Hard, and it is usually modeled as single objective optimization problem when modeling. For multi-objective optimization model, most researches consider two objectives. A multi-objective mathematical model for VRP is proposed, which considers the number of vehicles used, the length of route and the time arrived at each client. Genetic algorithm is one of the most widely used algorithms to solve VRP. As a type of genetic algorithm （GA）, non-dominated sorting in genetic algorithm-Ⅱ （NSGA-Ⅱ） also suffers from premature convergence and enclosure competition. In order to avoid these kinds of shortage, a greedy NSGA-Ⅱ （GNSGA-Ⅱ） is proposed for VRP problem. Greedy algorithm is implemented in generating the initial population, cross-over and mutation. All these procedures ensure that NSGA-Ⅱ is prevented from premature convergence and refine the performance of NSGA-Ⅱ at each step. In the distribution problem of a distribution center in Michigan, US, the GNSGA-Ⅱ is compared with NSGA-Ⅱ. As a result, the GNSGA-Ⅱ is the most efficient one and can get the most optimized solution to VRP problem. Also, in GNSGA-Ⅱ, premature convergence is better avoided and search efficiency has been improved sharply.

关键词： Greedy non-dominated sorting in genetic algorithm-Ⅱ (GNSGA-Ⅱ) Vehicle routing problem (VRP) Multi-objective optimization

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跨文化视角下文旅产品包装设计研究

鞋类工艺与设计 2024年第3期4卷 78-80页

作者：徐文慧陶诚湖北商贸学院湖北武汉430070

在当前全球化趋势逐渐推进的背景下,各国之间的交流逐步变得密切,对于文旅产品而言,包装设计既要满足不同背景下的文化要素融合,又要满足消费者的心理需求。立足于跨文化视角,系统性地论述了包装设计相关内容,分析了跨文化元素融入文旅... 详细信息

在当前全球化趋势逐渐推进的背景下,各国之间的交流逐步变得密切,对于文旅产品而言,包装设计既要满足不同背景下的文化要素融合,又要满足消费者的心理需求。立足于跨文化视角,系统性地论述了包装设计相关内容,分析了跨文化元素融入文旅产品包装设计中的价值,提出了文旅产品包装设计优化的策略,以期为文旅产品包装设计提供借鉴。

关键词：跨文化文旅产品包装设计

干扰素调节因子3促结直肠癌细胞增殖与侵袭相关探索

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上海交通大学学报（医学版） 2024年第3期44卷 301-311页

作者：徐文晖杨畅李瑞卿卞京李夏伊郑磊贞上海交通大学医学院附属新华医院肿瘤科上海200020

目的·分析结直肠癌中干扰素调节因子3(interferon regulatory factor 3,IRF3)表达水平与临床病理特征及患者预后的关系,观察IRF3过表达对结直肠癌细胞增殖与侵袭能力的影响及其相关蛋白通路。方法·下载癌症基因组图谱(The Cancer Geno... 详细信息

目的·分析结直肠癌中干扰素调节因子3(interferon regulatory factor 3,IRF3)表达水平与临床病理特征及患者预后的关系,观察IRF3过表达对结直肠癌细胞增殖与侵袭能力的影响及其相关蛋白通路。方法·下载癌症基因组图谱(The Cancer Genome Atlas,TCGA)数据,分析IRF3表达水平与恶性肿瘤患者(肾癌、结直肠癌、肝癌、前列腺癌)预后的关系。采用免疫组织化学法检测10例结直肠癌或肾癌患者的癌组织与癌旁正常组织切片中IRF3表达水平的差异。针对IRF3蛋白的C端残基位点进行改造,构建拟磷酸化IRF3-5D(396/398/402/404/405-D)高表达的HEK-293T细胞。分别在细胞培养12、24 h时,采用TANK结合激酶1(TANK-binding kinase 1,TBK1)抑制剂进行处理,并采用蛋白质印迹法检测细胞IRF3、p-IRF3(Ser386)蛋白表达水平。采用RNA测序技术探索IRF3-5D高表达与肿瘤相关蛋白表达水平的相关性。构建野生型IRF3(IRF3-WT)和IRF3-5D过表达的结直肠癌细胞CT26、COLON26,采用细胞计数法、细胞划痕试验和克隆形成试验检测细胞增殖及迁移能力。结果·TCGA数据分析提示癌组织中IRF3蛋白表达水平与患者的不良预后呈正相关。癌症患者病理组织免疫组织化学法显示,结直肠癌、肾癌组织中IRF3的表达水平显著上调,且蛋白表达集中于细胞核内。TBK1抑制剂分别在细胞培养12、24 h时间点作用后,HEK-293T细胞p-IRF3(Ser386)蛋白表达减弱。RNA测序和蛋白质印迹法结果显示,多个与癌症预后不良相关的蛋白[IRF9、细胞程序性死亡-配体1(programmed cell death 1-ligand 1,PD-L1)等]表达水平在IRF3-5D高表达的条件下显著上调。结直肠癌细胞中过表达IRF3-5D,可导致癌细胞的增殖、迁移能力显著上调。结论·结直肠癌中IRF3表达水平与患者不良预后呈正相关。IRF3-5D蛋白在结直肠癌细胞内高表达后,促进癌细胞恶性生物学行为。此外,IRF3-5D依赖于TBK1介导的IRF3活化激活通路,并上调多个肿瘤相关蛋白的表达水平。

关键词：结直肠恶性肿瘤靶向治疗干扰素调节因子3 TANK结合激酶1 RNA测序