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Acetylcholinesterase inhibitors and antioxidants mining from marine fungi:bioassays,bioactivity coupled LC-MS/MS analyses and molecular networking

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Marine Life Science & Technology 2020年第4期2卷 386-397页

作者： Yingying Nie wencong yang Yayue Liu Jingming yang Xiaoling Lei William HGerwick Yi Zhang Center for Marine Drugs Research and Development Shenzhen Institute of Guangdong Ocean UniversityShenzhen 518000China College of Food Science and Technology Key Laboratory of Guangdong Province Aquatic Products Processing and SafetyGuangdong Province Engineering Laboratory for Marine Biological ProductsGuangdong Provincial Engineering Technology Research Center of Marine FoodKey Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education InstitutionZhanjiang Municipal Key Laboratory of Marine Drugs and Nutrition for Brain HealthResearch Institute for Marine Drugs and NutritionGuangdong Ocean UniversityZhanjiang 52408&China Center for Marine Biotechnology and Biomedicine Scripps Institution of OceanographyUniversity of CaliforniaSan DiegoLa JollaCA 92093USA Collaborative Innovation Center of Seafood Deep Processing Dalian Polytechnic UniversityDalian 116034China

Marine fungi are potentially important resources for bioactive lead compounds for discovering new drugs for diseases such as Alzheimer's *** this paper,the combined bioassay model of acetylcholinesterase(AChE)inhibition,1,1-diphenyl-2-picrylhydrazyl(DPPH)free radical scavenging,and Artemi ci larval lethality was used to evaluate the activity and toxicity of 35 n narine fun gal strains from seas around *** bioactive constitue nfs were revealed by thin layer chromatography(TLC)autography,bioactivity coupled LC-MS/MS and Global Natural Products Social Molecular Networking(GNPS).The results show that the extracts of five strains exhibited higher AChE inhibition ratios than the positive control compound,'tacrine;for which the ratio was 89.8%at 200 pig/*** strains displayed both AChE inhibition(inhibition ratios>20%at 200 pg/ml)and DPPH scavenging activity(scavenging ratios>30%at 200 pg/ml)together with low Artemia larval toxicity(lethal rates<12%).TLC autography showed that the fractioned extracts of four strains contained highly diverse and different bioactive constituents,includi ng strains Talaromyces sp.C2\-\,Aspergillus ter re us C23-3,Trichoderma harzianum DLEN2008005,and Penicillium corylophilum *** the most potent sample F-l 1-1-b(derived from Aspergillus tern us C23-3),five AChE inhibitors and seven antioxida nts were recognized as bioactive molecules by AChE coupled ultrafiltration followed by LC-MS/MS,and LC-MS/MS coupled with DPPH ***,with the aid of GNPS,the AChE inhibitors were plausibly annotated as territrem analogues including territrems A-C/D,arisugacin A and an unknown compound 4,and the seven antioxidants were assigned as butyrolactone *** E,a phenolic derivative and possibly unknown compounds 8-10 and 12.

关键词： Acetylcholinesterase inhibition Antioxida Marine fungi LC-MS/MS Molecular networking

Peniazaphilones A—I,Produced by Co-culturing of Mangrove Endophytic Fungi,Penicillium sclerotiorum THSH-4 and Penicillium sclerotiorum ZJHJJ-18

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Chinese Journal of Chemistry 2021年第12期39卷 3404-3412页

作者： wencong yang Jie Yuan Qi Tan Yan Chen Yujia Zhu Hongming Jiang Ge Zou Zhenming Zang Bo Wang Zhigang She School of Chemistry Sun Yat-Sen UniversityGuangzhouGuangdong 510275China Department of Biochemistry Zhongshan School of MedicineSun Yat-Sen UniversityGuangzhouGuangdong 510080China Key Laboratory of Tropical Disease Control Sun Yat-Sen UniversityMinistry of EducationGuangzhouGuangdong 510080China National R&D Center for Edible Fungus Processing Technology Henan UniversityKaifengHenan 475004China

Co-culturing the mangrove endophytic fungus Penicillium sclerotiorum THSH-4 with Penicillium sclerotiorum ZJHJJ-18 in PDB medium resulted in the production of nine new azaphilones derivatives,peniazaphilones A—I(1-9),and six known azaphilones derivatives(10-15).Their structures were elucidated by analysis of detailed spectroscopic data,single-crystal X-ray diffraction,ECD spectra,^(13)C NMR calculation and DP4+*** is noteworthy that compound 1 with an isoquinoline quinone moiety,was isolated from natural source for the first ***,compound 2 is the third reported phenylhydrazone derivative from *** the bioactivity assays,compounds 1,12,14 and 15(IC_(50)=4.71-17.64μmol/L)exhibited potent inhibition of LPS-induced NO release from RAW264.7 without obvious cytotoxicity within 50μmol/L compared to the positive control indomethacin(IC_(50)=35.27μmol/L).Moreover,compounds 1 and 13 displayed moderate to strong cytotoxicity to A549 and MDA-MB-435,with IC_(50) values in the range of 6.84-14.63μmol/*** compound 1 showed moderate broad-spectrum antibacterial activity.

关键词： Co-culture Natural products Azaphilones Structure elucidation Cytotoxicity

(+)/(-)-Mycosphatide A,a pair of highly oxidized polyketides with lipid-lowering activity from the mangrove endophytic fungus Mycosphaerella ***-DZG01

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Chinese Chemical Letters 2024年第9期35卷 210-213页

作者： Qi Tan Run-Zhu Fan wencong yang Ge Zou Tao Chen Jianying Wu Bo Wang Sheng Yin Zhigang She School of Chemistry Sun Yat-sen UniversityGuangzhou 510006China School of Pharmaceutical Sciences Sun Yat-sen UniversityGuangzhou 510006China

(±)-Mycosphatide A(1a/1b),a pair of highly oxidized enantiomeric polyketides featuring a unique5/5/6/5-fused tetracyclic ring system,were isolated from the mangrove endophytic fungus Mycosphaerella *** structures were established by extensive spectroscopic analyses,single crystal Xray diffraction,and experimental electronic circular dichroism(ECD)spectra *** plausible biosynthetic pathway of 1 was proposed,which involved the generation of a key spiro[4.5]decane ***(+)-1a and(-)-1b exhibited significant lipid-lowering activity in 3T3-L1 adipocytes model,with EC50values of 7.85±1.56 and 8.87±0.80μmol/L,respectively.

关键词： Mangrove endophytic fungus Mycosphaerella sp. Polyketide Highly oxidized Lipid-lowering activity

Griseofulvin enantiomers and bromine-containing griseofulvin derivatives with antifungal activity produced by the mangrove endophytic fungus Nigrospora ***1

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Marine Life Science & Technology 2024年第1期6卷 102-114页

作者： Ge Zou wencong yang Tao Chen Zhaoming Liu Yan Chen Taobo Li Gulab Said Bing Sun Bo Wang Zhigang She School of Chemistry Sun Yat-sen UniversityGuangzhou 510275China State Key Laboratory of Applied Microbiology Southern China Guangdong Provincial Key Laboratory of Microbial Culture Collection and ApplicationInstitute of MicrobiologyGuangdong Academy of SciencesGuangzhou 510070China School of Pharmacy Anhui Medical UniversityHefei 230032China Department of Chemistry Women University SwabiSwabi 23430Pakistan

Marine microorganisms have long been recognized as potential sources for drug *** was one of the first antifungal natural products and has been used as an antifungal agent for *** this study,12 new griseofulvin derivatives[(±)-1-2,(+)-3,(±)-4,10-12,and 14-15]and two new griseofulvin natural products(9 and 16)together with six known analogues[(-)-3,5-8,and 13]were isolated from the mangrove-derived fungus Nigrospora ***1 treated with 0.3%NaCl or 2%NaBr in rice solid *** 2D structures and absolute configurations were established by extensive spectroscopic analysis(1D and 2D NMR,HRESIMS),ECD spectra,computational calculation,DP4+analysis,and X-ray single-crystal *** 1-4 represent the first griseofulvin enantiomers with four absolute configurations(2S,6'S;2R,6'R;2S,6'R;2R,6'S),and compounds 9-12 represent the first successful production of brominated griseofulvin derivatives from fungi via the addition of NaBr to the culture *** the antifungal assays,compounds 6 and 9 demonstrated significant inhibitory activities against the fungi Colletotrichum truncatum,Microsporum gypseum,and Trichophyton mentagrophyte with inhibition zones varying between 28 and 41 mm(10μg/disc).The structure-activity relationship(SAR)was analyzed,which showed that substituents at C-6,C-7,C-6'and the positions of the carbonyl and double bond of griseofulvin derivatives significantly affected the antifungal activity.

关键词： Mangrove endophytic fungus Nigrospora sp. Griseofulvin Antifungal activity

A Case of Colon Cancer Surgery Complicated with Acute Myocardial Infarction

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Journal of Clinical and Nursing Research 2022年第6期6卷 158-166页

作者： Fang Huang Sicong yang yang Wu Kaiyu Tang wencong yang The University of Hong Kong-Shenzhen Hospital Shenzhen 518053Guangdong ProvinceChina The Seventh Affiliated Hospital Sun Yat-sen UniversityShenzhen 517108Guangdong ProvinceChina Dongguan First Hospital Affiliated to Guangdong Medical University Dongguan 523121Guangdong ProvinceChina

Objective:To investigate the treatment and prevention of acute myocardial infarction after colon cancer ***:A patient with acute anterior myocardial infarction who underwent stent implantation in the Seventh Affiliated Hospital,Sun Yat-sen University in 2017 was selected as the *** patient was admitted to the emergency department for stent implantation and took antiplatelet and lipid regulating drugs orally on time after the *** patient also had a history of colon *** lesion was resected,and the complication after operation was acute myocardial *** active and effective treatment and intervention,the treatment effect of the patient was ***:Urgent PTCA was performed,and 0.5 mg Tirofiban was injected into the coronary *** results of angiography showed that the blood flow of anterior descending branch recovered to grade ***:The condition of patients with myocardial infarction after colon cancer surgery changes *** combined with drug treatment can achieve a good prognosis,reduce mortality,and improve patients’cardiac function.

关键词： Colon cancer Acute myocardial infarction Treatment method Prevention method Functional level

6~8岁骨龄段女童体质量指数测量及体脂率状况评估分析

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中国儿童保健杂志 2024年第1期32卷 79-83页

作者：杨文聪翟爽北部战区总医院儿科辽宁沈阳110000 北部战区总医院门诊部辽宁沈阳110000

目的分析6~8岁骨龄段女童体质量指数(BMI)测量及体脂率状况,为针对性预防和治疗儿童肥胖提供科学依据。方法收集2022年1—7月于北部战区总医院接受肥胖筛查的968例6~8岁骨龄段女童体检资料,分析6~8岁骨龄段女童BMI、体脂率、营养学指标... 详细信息

目的分析6~8岁骨龄段女童体质量指数(BMI)测量及体脂率状况,为针对性预防和治疗儿童肥胖提供科学依据。方法收集2022年1—7月于北部战区总医院接受肥胖筛查的968例6~8岁骨龄段女童体检资料,分析6~8岁骨龄段女童BMI、体脂率、营养学指标与肥胖率情况,采用Logistic多因素回归分析明确6~8岁骨龄段女童的肥胖危险因素。结果6~8岁女童BMI、全身脂肪率、内脏脂肪等级比较,差异有统计学意义(F=10.196、68.327、66.584,P3次/周(OR=1.432,95%CI:1.172~1.749)、进食甜食频率>3次/周(OR=2.670,95%CI:1.170~6.093)、进食速度<15min/餐(OR=1.366,95%CI:1.108~1.685)、户外活动频率<30min/d(OR=2.083,95%CI:1.162~3.736)、父母不了解或略了解营养学(OR=1.721,95%CI:1.129~2.623)是6~8岁女童肥胖的独立危险因素(P<0.05)。结论6~8岁骨龄段女童肥胖率均较高,需要通过科学搭配饮食、合理运动及引导父母提升营养学知识水平等方面进行改善。

关键词： 6~8岁骨龄段女童体质量指数体脂率肥胖

PREVENTIVE EFFECTS ON SENILE ANEMIA IN RATS BY MOXIBUSTION

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World Journal of Acupuncture-Moxibustion 1995年第2期5卷 11-14页

作者： Huang Cheng,Chen Hanping, Zhao Cuiying, Gu wencong,yang HuijunShanghai Research Institute of Acupuncture and Meridian, Shanghai 200030, China

The aim of the present study is to observe the preventive effects of moxibustion onseniIe anemia in rats and certify the anti-aging effects of moxibustion. The results show that thehemoglobin in 20 months old rats is lower than that in young rats, and the osmotic fragility and thehematocrit are higher than those in young rats. The hernoglobin in moxibustion group rats is signifi-cantly higher than that in old group, and the osmotic fragility and the hematocrit are markedly lowerthan those in old group, suggesting that moxibustion can delay the age-related changes of erythrocytein the rat and decrease the incidence rate of senile anemia of rat.

关键词： Moxibustion Preventive effect Aging Senile anemia

EZH2-dependent myelination following sciatic nerve injury

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Neural Regeneration Research 2025年第8期20卷 2382-2394页

作者： Hui Zhu Li Mu Xi Xu Tianyi Huang Ying Wang Siyuan Xu Yiting Wang wencong Wang Zhiping Wang Hongkui Wang Chengbin Xue Research Center of Clinical Medicine Affiliated Hospital of Nantong UniversityKey Laboratory of Neuroregeneration of Jiangsu and Ministry of EducationCo-innovation Center of NeuroregenerationNMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology ProductsNantong UniversityNantongJiangsu ProvinceChina Medical School of Nantong University NantongJiangsu ProvinceChina Department of Neurology Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Department of Rehabilitation Medicine Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Department of Critical Care Medicine Nantong Fourth People’s HospitalNantongJiangsu ProvinceChina

Demyelination and remyelination have been major focal points in the study of peripheral nerve regeneration following peripheral nerve ***,the gene regulatory network of regenerated myelin differs from that of native *** of enhancer of zeste homolog 2(EZH2)hinders the differentiation,maturation,and myelination of Schwann cells in *** further determine the role of EZH2 in myelination and recovery post-peripheral nerve injury,conditional knockout mice lacking Ezh2 in Schwann cells(Ezh2^(fl/fl);Dhh-Cre and Ezh2^(fl/fl);Mpz-Cre)were *** results show that a significant proportion of axons in the sciatic nerve of Ezh2-depleted mice remain *** highlights the crucial role of Ezh2 in initiating Schwann cell ***,we observed that 21 days after inducing a sciatic nerve crush injury in these mice,most axons had remyelinated at the injury site in the control nerve,while Ezh2^(fl/fl);Mpz-Cre mice had significantly fewer remyelinated axons compared with their wild-type *** suggests that the absence of Ezh2 in Schwann cells impairs myelin formation and *** conclusion,EZH2 has emerged as a pivotal regulatory factor in the process of demyelination and myelin regeneration following peripheral nerve *** EZH2 activity during these processes may offer a promising therapeutic target for the treatment of peripheral nerve injuries.

关键词： demyelination EZH2 myelination peripheral nerve injury PRC2 remyelination Schwann cells sciatic nerve crush sciatic nerve transection

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大冶市土壤重金属空间分布特征及其来源研究

安全与环境工程 2020年第4期27卷 121-127,142页

作者：杨文聪祁士华邢新丽中国地质大学(武汉)生物地质与环境地质国家重点实验室湖北武汉430074 中国地质大学(武汉)环境学院湖北武汉430074

为摸清国家级重金属重点防控区大冶市土壤重金属污染状况,选取大冶市及其周边地区作为研究区,利用空间分布分析、来源分析和污染状况评价等方法,探究研究区不同区域土壤重金属的空间分布特征及其来源。结果表明:研究区土壤中重金属元素A... 详细信息

为摸清国家级重金属重点防控区大冶市土壤重金属污染状况,选取大冶市及其周边地区作为研究区,利用空间分布分析、来源分析和污染状况评价等方法,探究研究区不同区域土壤重金属的空间分布特征及其来源。结果表明:研究区土壤中重金属元素As、Cd、Pb、Zn、Hg、Cu的平均含量明显高于湖北省土壤环境背景值,土壤中重金属元素As、Cd、Zn、Cu的平均含量普遍超出《土壤环境质量农用地土壤污染风险管控标准(试行)》;研究区不同区域间土壤中重金属元素含量有明显的差异,呈现出东西港区域>冶炼厂区域>大冶湖区域的趋势;研究区土壤中重金属元素As、Cd、Zn含量的高值区分布在东西港区域,土壤中重金属元素Cu、Pb、Hg含量的高值区分布在有色冶炼厂区域和东西港区域;研究区土壤重金属的来源主要是该地区有色金属冶炼厂等工业生产排放的废水和废气造成的东西港区域的水型污染和冶炼厂区域的气型污染。

关键词：土壤重金属污染空间分布来源分析大冶市