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Therapeutic efficiency of tissue-engineered human corneal endothelium transplants on rabbit primary corneal endotheliopathy

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第6期12卷 492-498页

作者： ting-jun fan Jun ZHAO Xiu-zhong HU Xi-ya MA wen-bo ZHANG Chao-zhong YANG Key Laboratory for Corneal Tissue Engineering College of Marine Life Sciences Ocean University of China Qingdao 266003 China Qingdao Dongfang Eye Hospital Qingdao 266110 China

To evaluate the therapeutic efficiency of tissue-engineered human corneal endothelia (TE-HCEs) on rabbit primary corneal endotheliopathy (PCEP),TE-HCEs reconstructed with monoclonal human corneal endothelial cells (mcHCECs) and modified denuded amniotic membranes (mdAMs) were transplanted into PCEP models of New Zealand white rabbits using penetrating *** TE-HCEs were examined using diverse techniques including slit-lamp biomicroscopy observation and pachymeter and tonometer measurements in vivo,and fluorescent microscopy,alizarin red staining,paraffin sectioning,scanning and transmission electron microscopy observations in *** corneas of transplanted eyes maintained transparency for as long as 200 d without obvious edema or immune *** corneal thickness of transplanted eyes decreased gradually after transplanting,reaching almost the thickness of normal eyes after 156 d,while the TE-HCE non-transplanted eyes were turbid and showed obvious corneal *** polygonal corneal endothelial cells in the transplanted area originated from the TE-HCE *** intact monolayer corneal endothelium had been reconstructed with the morphology,cell density and structure similar to those of normal rabbit corneal *** conclusion,the transplanted TE-HCE can reconstruct the integrality of corneal endothelium and restore corneal transparency and thickness in PCEP *** TE-HCE functions normally as an endothelial barrier and pump and promises to be an equivalent of HCE for clinical therapy of human PCEP.

关键词： Tissue-engineered human corneal endothelium New Zealand white rabbits Penetrating keratoplasty Primary corneal endotheliopathy Transparency

Structural optimization strategy of pipe isolation tool by dynamic plugging process analysis

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Petroleum Science 2021年第6期18卷 1829-1839页

作者： ting-ting Wu Hong Zhao Bo-Xuan Gao fan-Bo Meng College of Mechanical and Transportation Engineering China University of PetroleumBeijing102249China

During the pipeline plugging process,both the pipeline and the pipe isolation tool(PIT)will be greatly damaged,due to the violent vibration of the flow *** this study,it was proposed for the first time to reduce the vibration of the flow field during the plugging process by optimizing the surface structure of the ***,the central composite design(CCD)was used to obtain the optimization schemes,and the drag coefficient and pressure coefficient were proposed to evaluate the degree of flow field ***,a series of computational fluid dynamics(CFD)simulations were performed to obtain the drag coefficient and pressure coefficient during dynamic *** the mathematical model of drag coefficient and pressure coefficient with the surface structure of the PIT were established ***,a modified particle swarm optimization(PSO)was applied to predict the optimal value of the surface structure of the ***,an experimental rig was built to verify the effectiveness of the *** results showed that the improved method could reduce the flow field vibration by 49.56%.This study provides a reference for the design of the PIT surface structure for flow field vibration technology.

关键词： Pipe isolation tool Dynamic analysis Drag coefficient Pressure coefficient Modified particle swarm optimization algorithm

Cytotoxic effect and possible mechanisms of Tetracaine on human corneal epithelial cells in vitro

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International Journal of Ophthalmology(English edition) 2016年第4期9卷 497-504页

作者： Xin Pang ting-Jun fan Key Laboratory for Corneal Tissue Engineering College of Marine Life Sciences Ocean University of China

AIM： To demonstrate the cytotoxic effect and possible mechanisms of Tetracaine on human corneal epithelial（HCEP） cells in vitro.·METHODS： In vitro cultured HCEP cell were treated with Tetracaine hydrochloride at different doses for different times, and their morphology, viability, and plasma membrane permeability were detected by light microscopy, methyl thiazolyl tetrazolium（MTT） assay,and acridine orange（AO）/ethidium bromide（EB） staining,respectively. Their cell cycle progression, phosphati-dylserine orientation in plasma membrane, and mitochondrial membrane potential（MTP） were assessed by flow cytometry. DNA fragmentation, ultrastructure,caspase activation, and the cytoplasmic apoptosis inducing factor（AIF） and cytochrome c（Cyt. c） along with the expression of B-cell lymphoma-2（Bcl-2） family proteins were examined by gel electrophoresis,transmission electron microscope, enzyme linked immunosorbent assay（ELISA）, and Western blot,respectively.·RESULTS： After exposed to Tetracaine at doses from10.0 to 0.3125 g/L, the HCEP cells showed dose- and time-dependent morphological abnormality and typical cytopathic effect, viability decline, and plasma membrane permeability elevation. Tetracaine induced phosphati-dylserine externalization, DNA fragmentation, G1 phase arrest, and ultrastructural abnormality and apoptotic body formation. Furthermore, Tetracaine at a dose of0.3125 g/L also induced caspase-3,-9 and-8 activation,MTP disruption, up-regulation of the cytoplasmic amount of Cyt. c and AIF, the expressions of Bax and Bad, and down-regulation of the expressions of Bcl-2 and Bcl-x L.·CONCLUSION： Tetracaine above 0.3125 g/L（1/32 of its clinical applied dosage） has a dose- and time-dependent cytotoxicity to HCEP cells in vitro, with inducing cellapoptosis via a death receptor-mediated mitochondriondependent pathway.

关键词： Tetracaine cytotoxicity human cornel epithelial cells apoptosis mitochondrion

基于扎根理论的民办高校教师组织认同的影响因素研究

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黑龙江高教研究 2025年第1期43卷 124-134页

作者：范伟西安翻译学院陕西西安710105

组织认同反映的是教师个体对其所在学校的支持度和认同度,对减少教师流失、强化教师队伍建设具有重要现实意义。运用扎根理论方法,对19名民办高校教师的访谈资料进行编码分析,探究了影响民办高校教师组织认同的影响因素。研究表明,工作... 详细信息

组织认同反映的是教师个体对其所在学校的支持度和认同度,对减少教师流失、强化教师队伍建设具有重要现实意义。运用扎根理论方法,对19名民办高校教师的访谈资料进行编码分析,探究了影响民办高校教师组织认同的影响因素。研究表明,工作压力、组织声誉、组织支持、感知到的领导力等外部刺激因素,加剧了民办高校教师个体的工作不安全感、相对剥夺感和身心耗竭,进而影响了民办高校教师的组织认同水平。建议从政府、学校和教师等三个层面,不断优化政策供给,构建优良校园生态,培育教育情怀,以提升民办高校教师组织认同。

关键词：民办高校教师组织认同扎根理论刺激-有机体-反应(S-O-R)理论

Teleportation of a Kind of Three-Mode Entangled States of Continuous Variables

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Communications in Theoretical Physics 2005年第5X期44卷 833-836页

作者： fan Hong-Yi LIANG Xian-ting Department of Physics Shanghai Jiao Tong University Shanghai 200030 China Department of Material Science and Engineering University of Science and Technology of China Hefei 230026 China Department of Physics and Institute of Modern Physics Ningbo University Ningbo 315211 China

A quantum teleportation scheme to teleport a kind of tripartite entangled states of continuous variables by using a quantum channel composed of three bipartite entangled states is proposed. The joint Bell measurement is feasible because the bipartite entangled states are complete and the squeezed state has a natural representation in the entangled state basis. The calculation is greatly simplified by using the Schmidt decomposition of the entangled states.

关键词： three-mode entangled state of continuous variables teleportation squeezed state

Quantum Fluctuation in Mesoscopic Coupled LC Electric Circuits at Finite Temperature

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Communications in Theoretical Physics 2001年第3期35卷 271-274页

作者： Liang Xian-ting fan Hong-Yi Department of Material Science and Engineering University of Science and Technology of China Hefei 230026 China

We consider the quantization of two coupled LC circuits with mutual inductance at a finite temperature T. It is shown that the quantum mechanical zero-point fluctuations of currents in the two circuits both increase with upgoing T. Thermal field dynamics and Weyl-Wigner theorern are used in our calculation of ensemble average of the observables.

关键词： LC电路量子涨落介观耦合

Egg tanning improves the efficiency of CRISPR/Cas9-mediated mutant locust production by enhancing defense ability after microinjection

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Journal of Integrative Agriculture 2021年第10期20卷 2716-2726页

作者： ZHANG ting-ting wen ting-mei YUE Yang YAN Qiang DU Er-xia fan San-hong Siegfried ROTH LI Sheng ZHANG Jian-zhen ZHANG Xue-yao ZHANG Min Research Institute of Applied Biology Shanxi UniversityTaiyuan 030006P.R.China School of Life Science Shanxi UniversityTaiyuan 030006P.R.China State Key Laboratory of Cotton Biology/Key Laboratory of Plant Stress Biology School of Life SciencesHenan UniversityKaifeng 475004P.R.China Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology Institute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhou 510631P.R.China Institute for Developmental Biology University of CologneCologne 50674Germany

The mutant efficiency and hatching ratio are two key factors that significantly affect the construction of genome-modified mutant *** the construction of CRISPR/Cas9-mediated dsLmRNase2^(–/–)mutant locusts,we found that the tanned eggs which experienced a 20-min contact with the oocyst exhibited a higher success rate compared to fresh newly-laid eggs that were less ***,the heritable efficiency of the dsLmRNase2 deletion to the next generation G_(1 )progeny was similar between adults derived from the tanned or less tanned engineered ***,the similar effective mutant ratios in the normally developed eggs and G_(0) adults of tanned and less tanned eggs also indicated that tanning did not reduce the absolute mutation efficiency induced by CRISPR/***,we found that the syncytial division period,which was longer than the time for tanning,conferred a window period for microinjection treatment with efficient mutation in both tanned and less tanned *** further found that tanned eggs exhibited a higher hatching rate due to a reduced infection rate following *** the anti-pressure and ultrastructure analyses indicated that the tanned eggs contained compressed eggshells to withstand increased external *** summary,tanned eggs possess stronger defense responses and higher efficiency of genome editing,providing an improved model for developing Cas9-mediated gene editing procedures in locusts.

关键词： brown tanned eggs CRISPR/Cas9 mutant locusts microinjection defense ability

Quantum Fluctuation in Thermal Vacuum State for Mesoscopic LC Electric Circuit

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Chinese Physics Letters 2000年第3期17卷 174-176页

作者： fan Hong-Yi LIANG Xian-ting Department of Applied Physics Shanghai Jiao Tong UniversityShanghai 200030 Department of Material Science and Engineering University of Science and Technology of ChinaHefei 230026 Department of Physics Huaihua Teachers CollegeHuaihuaj Hu'nan 418008

We consider the quantization of LC(inductance-capacitance)circuit at a finite temperature T as any practical circuits always produce Joule heat except for *** is shown that the quantum mechanical zeropoint fluctuation... 详细信息

关键词： quantum capacitance superconductivity

Maternal sleep deprivation disrupts glutamate metabolism in offspring rats

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Zoological Research 2024年第6期45卷 1221-1231页

作者： wen-ting He Dong-Xu Li Jin-Hua fan Zheng-Yu Yao Yu-Peng Cun Zhi-fang Dong Growth Developmentand Mental Health of Children and Adolescence CenterPediatric Research InstituteMinistry of Education Key Laboratory of Child Development and DisordersNational Clinical Research Center for Child Health and DisordersChongqing Key Laboratory of Child Neurodevelopment and Cognitive DisordersChildren's Hospital of Chongqing Medical UniversityChongqing 400014China

Maternal sleep deprivation(MSD)has emerged as a significant public health concern,yet its effects on offspring metabolism remain poorly *** study investigated the metabolomic implications of MSD on offspring cognitive development,with a particular focus on alterations in glutamate *** rats were subjected to sleep deprivation during late *** and brain samples from their offspring were collected at different postnatal days(P1,P7,P14,and P56)and analyzed using untargeted metabolomics with liquid chromatography-mass *** analysis revealed significant differences in various amino acids,including L-glutamate,L-phenylalanine,L-tyrosine,and L-tryptophan,which are crucial for cognitive *** differential analysis and partial least squares discriminant analysis(sPLS-DA)demonstrated a gradual reduction in these metabolic differences in the brain as the offspring underwent growth and *** pathway analysis revealed differential regulation of several pathways,including alanine,aspartate,and glutamate metabolism,glutathione metabolism,arginine biosynthesis,aminoacyl-tRNA biosynthesis,histidine metabolism,and taurine and hypotaurine metabolism,at different developmental *** and Spearman analyses indicated that the observed changes in metabolites in MSD progeny may be related to various gut microbes,Ruminococcus_1,Ruminococcaceae_UCG-005,and Eubacterium_coprostanoligenes_*** assays further demonstrated developmental changes in the L-glutamate metabolic ***,these findings suggest that MSD not only affects maternal wellbeing but also has enduring metabolic consequences for offspring,particularly impacting pathways linked to cognitive *** highlights the importance of addressing maternal sleep health to mitigate potential long-term consequences for offspring.

关键词： Maternal sleep deprivation Glutamate metabolism Metabolomics Cognitive development Offspring