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Pre-and post-pollination barriers between two exotic and five native Sagittaria species:Implications for species conservation

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Plant Diversity 2023年第4期45卷 456-468页

作者： ting-ting Zou Sen-Tao Lyu Qi-Lin Jiang Shu-He Shang Xiao-fan Wang Laboratory of Plant Systematics and Evolutionary Biology College of Life SciencesWuhan UniversityWuhan 430072China

Anthropogenic introduction of species has resulted in a breakdown of geographical barriers and hybridization in previously allopatric ***,examining hybridization proneness of exotic species contributes to revealing its potential ***,reproductive barriers may be strengthened or weakened due to long-term geographical isolation for these newly sympatric ***,few studies have evaluated multiple barriers between alien and native *** this study,we quantified the importance of four pre-pollination barriers(phenological,floral traits,pollen production,and floral constancy)and four post-pollination barriers(pollen-pistil incompatibility,seed set,seed viability,and seedling survival)between two introduced and five native Sagittaria *** showed that introduced *** was cross-compatible with two native species,whereas introduced *** was incapable of hybridizing with any native *** barriers were asymmetric within species pairs and multiple barriers acted in concert to maintain species *** barriers contributed more to total reproductive isolation in native species,whereas prepollination barriers played a stronger role in total reproductive isolation for two introduced *** set was the only barrier that was positively correlated with genetic *** results provide a perspective to better understand reproductive barriers for secondary contact *** highlight the importance of monitoring hybridization events before human introduction and the possible conservation strategies to remove invasive species with hybridization proneness.

关键词： Alismataceae Introduced species Conservation Hybridization Reproductive isolation Sagittaria

Proteome analysis of round-headed and normal spermatozoa by 2-D fluorescence difference gel electrophoresis and mass spectrometry

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Asian Journal of Andrology 2009年第6期11卷 683-694页

作者： ting-ting Liao Zhen Xiang wen-Bing Zhu Li-Qing fan Institute of Human Reproduction and Stem Cell Engineering Central South University Changsha 410078 China

Globozoospermia is a severe form of teratozoospermia characterized by round-headed spermatozoa with an absent acrosome, an aberrant nuclear membrane and midpiece defects. Globozoospermia is diagnosed by the presence of 100% round-headed spermatozoa on semen analysis, and patients with this condition are absolutely infertile. The objective of this study was to investigate the differences in protein expression between human round- headed and normal spermatozoa. Two-dimensional （2-D） fluorescence difference gel electrophoresis （DIGE） coupled with mass spectrometry （MS） was used in this study. Over 61 protein spots were analysed in each paired normal/round-headed comparison, using DIGE technology along with an internal standard. In total, 35 protein spots identified by tandem mass spectrometry （MS/MS） exhibited significant changes （paired t-test, P 〈 0.05） in the expression level between normal and round-headed spermatozoa. A total of nine proteins were found to be upregulated and 26 proteins were found to be downregulated in round-headed spermatozoa compared with normal spermatozoa. The differentially expressed proteins that we identified may have important roles in a variety of cellular processes and structures, including spermatogenesis, cell skeleton, metabolism and spermatozoa motility.

关键词： differential protein globozoospermia mass spectrometry (MS) two-dimensional difference gel electrophoresis (2-D DIGE)

In situ-prepared homogeneous supramolecular organic framework drug delivery systems(sof-DDSs)：Overcoming cancer multidrug resistance and controlled release

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Chinese Chemical Letters 2017年第4期28卷 798-806页

作者： Jia Tian Chi Yao wen-Lin Yang Lei Zhang Dan-Wei Zhang Hui Wang fan Zhang Yi Liu Zhan-ting Li Collaborative Innovation Center of Chemistry for Energy Materials (iChEM) Department of Chemistry Fudan University Shanghai 200433 China Department of Pathology School of Basic Medical Sciences Fudan University Shanghai 200032 China The Molecular Foundry Lawrence Berkeley National Laboratory Berkeley 94720 United States

Water-soluble three-dimensional porous supramolecular organic frameworks（SOFs） have been demonstrated as a new generation of homogeneous polycationic platforms for anti-cancer drug *** new SOF drug delivery systems（sof-DDSs） can adsorb dianionic pemetrexed（PMX）,a clinically used chemotherapeutic agent instantaneously upon dissolving in water,which is driven by both electrostatic attraction and *** in situ-prepared PMX@SOFs are highly stable and can avoid important release of the drug during plasm circulation and overcome the multidrug resistance of human breast MCF-7/Adr cancer cells to enter the cancer *** microenvironment of cancer cells promotes the release of the drug in cancer *** in vitro and in vivo studies have revealed that sofDDSs considerably improve the treatment efficacy of PMX,leading to 6-12-fold reduction of the IC50 values,as compared with that of PMX *** new drug delivery strategy omits the loading process required by most of reported nanoparticle-based delivery systems and thus holds promise for future development of low-cost drug delivery systems

关键词： Supramolecular organic framework In situ preparation Drug delivery Controlled release Pemetrexed Human breast cancer Multidrug resistance

A sleep staging model for the sleep environment control based on machine learning

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Building Simulation 2023年第8期16卷 1409-1423页

作者： ting Cao Zhiwei Lian Heng Du Jingyun Shen Yilun fan Junmeng Lyu School of Design Shanghai Jiao Tong UniversityShanghai 200240China

To date,dynamic sleep environment has been attracted the focus of *** to the individual difference on sleep phase and thermal comfort,changes in sleep environment should be occupant-centered,and precise regulation of the environment required current sleep ***,few studies connected occupants and the environment through physiological signal-based model of sleep ***,this study tried to develop a data driven sleep staging model with higher accuracy through sleep experiments collecting *** database was processed and selected efficiently according to the characteristics of physiological ***,the sleep staging model with an average accuracy of 93.9%was built,and other mean indicators(precision:82.5%,recall:83.1%,F1 score:82.8%)performed *** features adopted by model were found to come from different brain regions,and the global brain signals were suggested to play an important role in the construction of sleep staging ***,the computational processing of physiology signals should consider their characteristics,i.e.,time domain,frequency domain,time-frequency domain and nonlinear *** model obtained in this study may deliver a credible reference to advance the research on control of sleep environment.

关键词： sleep environment sleep staging model physiological signals machine learning environmental control

The interactions between bloodstream and vascular structure on aortic dissecting aneurysmal model: A numerical study

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Acta Mechanica Sinica 2013年第3期29卷 462-468页

作者： Zeng-Sheng Chen Zhan-Ming fan Xi-wen Zhang Department of Engineering Mechanics School of AerospaceTsinghua University Institute of Heart Pulmonary & Vascular Diseases Capital University of Medical Science Affiliated Beijing Anzhen Hospital

Stent-graft implantation is an important means of clinical treatment for aortic dissecting aneurysm （ADA）. However, researches on fluid dynamics effects of stent were rare. Computer simulation was used to investigate the interactions between bloodstream and vascular structure in a stented ADA, which endures the periodic pulse velocity and pressure. We obtained and analyzed the flow velocity distribution, the wall displacement and wall stress in the ADA. By comparing the different results between a non-stented and a stented ADA, we found that the insertion of a vascular graft can make the location of maximum stress and displacement move from the aneurysm lumen wall to the artery wall, accompanied with a greatly decrease in value. These results imply that the placement of a stent-graft of any kind to oc-clude ADA will result in a decreased chance of rupture.

关键词： Aortic dissecting aneurysm (ADA) · Stent-graft · Fluid-structure interaction (FIS)

Transcriptome-wide identification and expression profiling of Pinus massoniana MYB transcription factors responding to phosphorus deficiency

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Journal of Forestry Research 2020年第3期31卷 909-919页

作者： Fuhua fan Qingzhu Wang Xiaopeng wen Guijie Ding Institute for Forest Resources and Environment of Guizhou Guizhou UniversityGuiyang 550025China College of Forestry Guizhou UniversityGuiyang 550025China Institute of Agro-Bioengineering Guizhou UniversityGuiyang 550025China

Myeloblastosis(MYB) proteins constitute one of the largest transcription factor(TF) families in plants and play crucial roles in regulating plant physiological and biochemical processes, including adaptation to diverse abiotic stresses. These TF families contain highly conserved MYB repeats(1 R, R2 R3, 3 R and 4 R) at the N-terminus. Roles for MYB TFs have been reported in response to such stresses as dehydration, salt, cold, and drought. The characterization of Masson pine(Pinus massoniana) MYB TFs are reported, including the analysis of MYB TFs expression in seedlings under controlled conditions and two different phosphate(Pi) deficient treatments. By searching for conserved MYB motifs in full transcriptomic RNA sequencing data for P. massoniana, 59 sequences were identified as MYB TFs. Conserved domainstructures and comparative functional and phylogenetic relationships of these MYB TFs with those in Arabidopsis were assessed using various bioinformatics tools. Based on microarray analysis, P. massoniana MYB genes exhibited different expression patterns under the two Pi deficiency conditions. Genes encoding MYB TFs that showed increased expression under critical Pi deficiency were identified, and some MYBs were differentially expressed only under conditions of severe Pi starvation. These results are useful for the functional characterization of MYB TFs that may be involved in the response to Pi deficiency and play divergent roles in plants.

关键词： MYB transcription factors Transcriptome Pinus massoniana Phosphorus deficiency Microarray analysis

Magnetically recoverable Fe3O4@polydopamine nanocomposite as an excellent co-catalyst for Fe^3+ reduction in advanced oxidation processes

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Journal of Environmental Sciences 2020年第6期32卷 69-78页

作者： Ling fan Jinliang Xie Zhilin Zhang Yaping Zheng Dongdong Yao ting Li Department of Applied Chemistry School of Natural and Applied ScienceNorthwestern Polytechnical UniversityXi'an 710072China

Advanced oxidation processes are widely applied to removal of persistent toxic substances from wastewater by hydroxyl radicals(·OH),which is generated from hydrogen peroxide(H2O2)***,their practical applications have been hampered by many strict conditions,such as iron sludge,rigid pH condition,large doses of hydrogen peroxide and Fe^2+,***,a magnetically recyclable Fe3O4@polydopamine(Fe3O4@PDA)coreshell nanocomposite was *** an excellent reducing agent,it can convert Fe^3+to Fe^2+.Combined with the coordination of polydopamine and ferric ions,the production of iron sludge is *** minimum concentration of hydrogen peroxide(0.2 mmol/L and Fe^2+(0.18 mmol/L))is 150-fold and 100-fold lower than that of previous reports,*** also exhibits excellent degradation performance over a wide pH range from 3.0 to *** after the tenth recycling,it still achieves over 99%degradation efficiency with the total organic carbon degradation rate of 80%,which is environmentally benign and has a large economic *** discovery paves a way for extensive practical application of advanced oxidation processes,especially in environmental remediation.

关键词： Fe3O4@polydopamine nanocomposites Fenton-like catalysts Fe^3+/Fe^2+cycle Magnetically reusable

Encapsulation of ultrafine Pd nanoparticles within the shallow layers of UiO-67 for highly efficient hydrogenation reactions

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Science China Chemistry 2021年第1期64卷 109-115页

作者： Zhiming Cui ting fan Liyu Chen Ruiqi fang Chuanmao Li Yingwei Li State Key Laboratory of Pulp and Paper Engineering School of Chemistry and Chemical EngineeringSouth China University of TechnologyGuangzhou 510640China Guangzhou Keneng Cosmetics Scientific Research Co. Lid.Guangzhou 510800China

Metal-organic frameworks(MOFs)have been used to encapsulate active metal nanoparticles(MNPs)to fabricate MNPs@MOFs composites with high catalytic ***,the diffusion of reactants and the accessibility of MNPs located in the center of MOFs may be hindered due to the inherent microporous structures of MOFs,which would affect the catalytic activities of ***,we report a solvent assisted ligand exchange-hydrogen reduction(SALE-HR)strategy to selectively encapsulate ultrafine MNPs(Pd or Pt)within the shallow layers of a MOF,i.e.,*** particle sizes of the encapsulated MNPs and the thickness of the MNPs-embedded layers can be adjusted easily by controlling the SALE conditions(*** and temperature).Crucially,the LE-Pd@UiO-80-0.5 composite with the thinnest Pd-embedded layers displays remarkable catalytic efficiency with a high turnover frequency(TOF)value of 600 h^-1towards hydrogenation of nitrobenzene under 1 atm H_2at room *** results indicate that the catalytic efficiency and the utilization of MNPs can be enhanced by compactly encapsulating MNPs within the shallow layers of MOFs as close to their outer surfaces as possible,owing to the short masstransfer distance and enhanced accessibility of overall MNPs.

关键词： Metal-organic frameworks metal nanoparticles heterogeneous catalysis encapsulation hydrogenation

Bounded Evaluation:Querying Big Data with Bounded Resources

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International Journal of Automation and computing 2020年第4期17卷 502-526页

作者： Yang Cao wen-Fei fan Teng-Fei Yuan University of Edinburgh Edinburg EH89ABUKUniversity of EdinburghEdinburg EH89ABUK Shenzhen Institute of Computing Sciences Shenzhen UniversityShenzhen 518060China Beijing Advanced Innovation Center for Big Data and Brain Computing Beihang UniversityBeijing 100191China

This work aims to reduce queries on big data to computations on small data,and hence make querying big data possible under bounded resources.A query Q is boundedly evaluable when posed on any big dataset D,there exists a fraction DQ of D such that Q(D)=Q(DQ),and the cost of identifying DQ is independent of the size of *** has been shown that with an auxiliary structure known as access schema,many queries in relational algebra(RA)are boundedly evaluable under the set semantics of *** paper extends the theory of bounded evaluation to RAaggr,i.e.,RA extended with aggregation,under the bag semantics.(1)We extend access schema to bag access schema,to help us identify DQ for RAaggr queries Q.(2)While it is undecidable to determine whether an RAaggr query is boundedly evaluable under a bag access schema,we identify special cases that are decidable and practical.(3)In addition,we develop an effective syntax for bounded RAaggr queries,i.e.,a core subclass of boundedly evaluable RAaggr queries without sacrificing their expressive power.(4)Based on the effective syntax,we provide efficient algorithms to check the bounded evaluability of RAaggr queries and to generate query plans for bounded RAaggr queries.(5)As proof of concept,we extend PostgreSQL to support bounded *** experimentally verify that the extended system improves performance by orders of magnitude.

关键词： Bounded evaluation resource-bounded query processing effective syntax access schema boundedness