Transcriptome-wide identification and expression profiling of Pinus massoniana MYB transcription factors responding to phosphorus deficiency
对磷缺乏作出回应的 Pinus massoniana MYB 抄写因素介绍的 Transcriptome 宽的鉴定和表示作者机构:Institute for Forest Resources and Environment of GuizhouGuizhou UniversityGuiyang 550025China College of ForestryGuizhou UniversityGuiyang 550025China Institute of Agro-BioengineeringGuizhou UniversityGuiyang 550025China
出 版 物:《Journal of Forestry Research》 (林业研究(英文版))
年 卷 期:2020年第31卷第3期
页 面:909-919页
核心收录:
学科分类:0907[农学-林学] 08[工学] 0829[工学-林业工程] 09[农学]
基 金:funded by the Natural Science Foundation of Guizhou Province,P.R.China(20161051) the National Natural Science Foundation of China(31660185) the Doctoral Fund Project of Guizhou University,P.R.China(201464)
主 题:MYB transcription factors Transcriptome Pinus massoniana Phosphorus deficiency Microarray analysis
摘 要:Myeloblastosis(MYB) proteins constitute one of the largest transcription factor(TF) families in plants and play crucial roles in regulating plant physiological and biochemical processes, including adaptation to diverse abiotic stresses. These TF families contain highly conserved MYB repeats(1 R, R2 R3, 3 R and 4 R) at the N-terminus. Roles for MYB TFs have been reported in response to such stresses as dehydration, salt, cold, and drought. The characterization of Masson pine(Pinus massoniana) MYB TFs are reported, including the analysis of MYB TFs expression in seedlings under controlled conditions and two different phosphate(Pi) deficient treatments. By searching for conserved MYB motifs in full transcriptomic RNA sequencing data for P. massoniana, 59 sequences were identified as MYB TFs. Conserved domainstructures and comparative functional and phylogenetic relationships of these MYB TFs with those in Arabidopsis were assessed using various bioinformatics tools. Based on microarray analysis, P. massoniana MYB genes exhibited different expression patterns under the two Pi deficiency conditions. Genes encoding MYB TFs that showed increased expression under critical Pi deficiency were identified, and some MYBs were differentially expressed only under conditions of severe Pi starvation. These results are useful for the functional characterization of MYB TFs that may be involved in the response to Pi deficiency and play divergent roles in plants.