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King cobra peptide OH-CATH30 as a potential candidate drug through clinic drug-resistant isolates

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Zoological Research 2018年第2期39卷 87-96页

作者： Feng Zhao Xin-Qiang Lan Yan Du Pei-Yi Chen Jiao Zhao Fang Zhao wen-hui lee Yun Zhang Key Laboratory of Subtropical Medicinal Edible Resources Development and Utilization in Yunnan Province Department of Biology and Chemistry Puer University Puer Yunnan 665000 China Key Laboratory of Bioactive Peptides of Yunnan Province/Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences Kunming Institute of Zoology Kunming Yunnan 650223 China Department of Clinical Laboratories First Affiliated Hospital of Kunming Medical University Kunming Yunnan 650032 China Institute of Comparative Study of Traditional Materia Medica Institute of Integrative Medicine of Fudan University Shanghai 200433 China

Cationic antimicrobial peptides （AMPs） are considered as important candidate therapeutic agents, which exert potent microbicidal properties against bacteria, fungi and some viruses. Based on our previous findings king cobra cathelicidin （OH-CATH） is a 34-amino acid peptide that exerts strong antibacterial and weak hemolytic activity. The aim of this research is to evaluate the efficacy of both OH-CATH30 and its analog D-OH-CATH30 against clinical isolates comparing with routinely utilized antibiotics in vitro. In this study, 584 clinical isolates were tested （spanning 2013-2016） and the efficacy of the candidate peptides and antibiotics were determined by a broth microdilution method according to the CLSI guidelines. Among the 584 clinical isolates, 85% were susceptible to OH-CATH30 and its analogs. Both L- and D-OH-CATH30 showed higher efficacy against （toward） Gram-positive bacteria and stronger antibacterial activity against nearly all Gram-negative bacteria tested compare with antibiotics. The highest bactericidal activity was detected against Acinetobacter spp., including multi-drug-resistant Acinetobacter baumannfi （MRAB） and methicillin-resistant Staphylococcus aureus （MRSA）. The overall efficacy of OH-CATH30 and its analogs was higher than that of the 9 routinely used antibiotics. OH-CATH30 is a promising candidate drug for the treatment of a wide variety of bacterial infections which are resistant to many routinely used antimicrobial agents.

关键词： Cathelicidin Antibacterial agent Clinicalisolates OH-CATH30

Protein composition of extracellular vesicles from skin secretions of the amphibian Bombina maxima

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Zoological Research 2022年第4期43卷 687-690页

作者： Xue-Song Wei Ling-Zhen Liu Xian-Ling Bian Lin Zeng wen-hui lee Ling Lin Yun Zhang Qi-Quan Wang Human Aging Research Institute and School of Life Science Nanchang Universityand Jiangxi Key Laboratory of Human AgingNanchangJiangxi 330031China Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences&Yunnan Province Kunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650201China College of Life Sciences Anhui Normal UniversityWuhuAnhui 241000China Center for Excellence in Animal Evolution and Genetics Chinese Academy of SciencesKunmingYunnan 650201China Institutional Center for Shared Technologies and Facilities of the Kunming Institute of Zoology Chinese Academy of SciencesKunmingYunnan 650201China

DEAR EDITOR,Extracellular vesicles(EVs)are important for the transport of biologically active materials and for intercellular *** an exposed mucosa,amphibian skin participates in many essential physiological *** date,however,little is known about EVs in amphibian ***,we successfully isolated EVs from the skin secretions of Bombina maxima,and characterized the EVs using nanoparticle tracking,western blotting,and electron microscopy.

关键词： maxima vesicles secretion

Cathelicidins from Amphibian and Reptile Species

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Cathelicidins from Amphibian and Reptile Species

2008年全国生物化学与分子生物学学术大会

作者： wen-hui lee Biotoxin Units Key Laboratory of Animal Models and Human Disease MechanismsKunming Institute of ZoologyThe Chinese Academy of SciencesKunming

Cathelicidins are cationic host defense peptides that play an important role in innate immune *** to now,most of the identified cathelicidins were from mammalian *** of the cathelicidin family were also found in chick...

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Comparative analysis of diverse toxins from a new pharmaceutical centipede,Scolopendra mojiangica

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Zoological Research 2020年第2期41卷 138-147页

作者： Zi-Chao Liu Jin-Yang Liang Xin-Qiang Lan Tao Li Jia-Rui Zhang Fang Zhao Geng Li Pei-Yi Chen Yun Zhang wen-hui lee Feng Zhao Key Laboratory of Ethnic Medical Resources Research and Southeast Asian International Cooperation of Yunnan Universities Department of Biology and ChemistryPuer UniversityPuerYunnan 665000China Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences&Yunnan Province Kunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650223China Engineering Research Center for Exploitation and Utilization of leech Resources in Universities of Yunnan Province School of Agronomy and Life SciencesKunming UniversityKunmingYunnan 650214China Key Laboratory of Active Molecules and Drug Development Puer UniversityPuerYunnan 665000China Kunming College of Life Science University of Chinese Academy of SciencesKunmingYunnan 650204China Institute of Comparative Study of Traditional Materia Medica Institute of Integrative Medicine of Fudan UniversityShanghai 200032China Nanshan College Guangzhou Medical UniversityGuangzhouGuangdong 511436China

As the oldest venomous animals,centipedes use their venom as a weapon to attack prey and for *** venom,which contains many bioactive and pharmacologically active compounds,has been used for centuries in Chinese medicine,as shown by ancient *** on comparative analysis,we revealed the diversity of and differences in centipede toxin-like molecules between Scolopendra mojiangica,a substitute pharmaceutical material used in China,and *** *** than 6000 peptides isolated from the venom were identified by electrospray ionization-tandem mass spectrometry(ESI-MS/MS)and inferred from the *** a result,in the proteome of ***,246 unique proteins were identified:one in five were toxin-like proteins or putative toxins with unknown function,accounting for a lower percentage of total proteins than that in *** mining identified approximately 10 times more toxin-like proteins,which can characterize the precursor structures of mature toxinlike ***,the constitution and quantity of the toxin transcripts in these two centipedes were *** toxicity assays,the crude venom showed strong insecticidal and hemolytic *** findings highlight the extensive diversity of toxin-like proteins in *** and provide a new foundation for the medical-pharmaceutical use of centipede toxin-like proteins.

关键词： Centipede Toxins Pharmaceutical use Proteotranscriptomic analysis

广州某高等医学院校研究生乙肝疫苗接种情况调查

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中华实验和临床病毒学杂志 2007年第2期21卷 114-116页

作者：林潮双朱建云麦丽 wen-hui lee 高志良中山大学附属第三医院感染科广州510630 Biostatistics Amgen Seattle WA 98119

目的了解广州某高等医学院校研究生乙肝疫苗接种情况，为乙肝防治工作提供依据。方法对广州某高等医学院校2006年入学的1139名研究生进行入学体检现况调查，采集血标本用ELISA法检测乙型肝炎表面抗原（HBsAg）和表面抗体（HBsAb）。同... 详细信息

目的了解广州某高等医学院校研究生乙肝疫苗接种情况，为乙肝防治工作提供依据。方法对广州某高等医学院校2006年入学的1139名研究生进行入学体检现况调查，采集血标本用ELISA法检测乙型肝炎表面抗原（HBsAg）和表面抗体（HBsAb）。同时发放乙肝疫苗接种情况调查表，调查乙肝疫苗接种的年代、次数，是否加强接种及时间。用SAS统计软件包对结果进行X^2检验分析。结果广州某高等医学院校06级研究生HBsAg阳性率为2．90％，曾注射乙肝疫苗组，HBsAg阳性率显著低于从未注射疫苗组（1．15％ vs． 21．69％，P〈0．0001），而HBsAb阳性率则显著高于从未注射疫苗组（81．54％ vs．44．58％，P〈0．0001）。有17．31％曾接种乙肝疫苗者未能达到预期预防效果。不同年龄研究生乙肝疫苗接种效果有差异（P=0．0462），随着年龄的增加，HBsAb阳性率有下降趋势。女性乙肝疫苗接种效果较男性好（80．0％ vs．84．87％，P=0．0468）。接种年限在3年内者，HBsAb阳性率较其他年限高（0—3年 vs．4—6年，P=0．0089；0—3年 vs．7—9年，P=0．0172；0—3年 vs．〉9年，P=0，0474）。注射大于3针（即加强接种）免疫效果较注射3针者好，差异有统计学意义（P=0．0093）。结论随着年龄的增加，乙肝疫苗接种效果（HBsAb阳性率）逐渐降低。男性群体较女性群体更易成为乙型肝炎病毒的易感人群。对接种年限大于3年者，可进行抗-HBs监测，及时进行加强免疫。

关键词：流行病学研究酶联免疫吸附测定学生,医科肝炎疫苗,乙型接种

Aerolysin-like pore-forming proteins in centipede species of Scoropendra subspinipes mutilans

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Aerolysin-like pore-forming proteins in centipede species of...

第十四届生物毒素毒理学术大会暨第一届生物毒素——从生存适应到转化医学专题学术会议

作者： Xin-Qiang Lan wen-hui lee Yun Zhang Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences/Key Laboratory of Bio-active Peptides of Yunnan Province Kunming Institute of Zoology

Scoropendra subspinipes mutilans(***) has been widely used in China for more than 2000 years as a traditional Chinese *** to the extraordinary chemical and pharmacological diversities,*** toxins have attracted increasing *** investigations on the species mainly focused on peptide toxins which reacted on ion channels and little was known about *** proteins,especially aerolysin-like pore-forming proteins(ALPs).Through the transcriptomic and proteomic analysis,we found 8 ALPs contained in *** primary structures of these ALPs were predicted by I-TASSER and all of them had aerolysin folding *** explore the role of ALPs in ***,we used 37℃,4℃and 8% O(low oxygen)to stimulate *** and explore that the expression patterns of each *** further explore the function of ALPs,prokaryotic expression technology was used to express the SSM-ALP-8 aerolysin domain,then successfully prepared its *** localization of ALPs in *** was detected by immunofluorescence and the results matched well with PCR ***,we have identified ALPs from *** *** work provides the basis for future research into the ALPs functions in ***.

关键词： Scoropendra subspinipes mutilans Aerolysin-like pore-forming proteins

Comparison of γ-bungarotoxin content in different regions and systematic LD＿(50) value determination of γ-bungarotoxin via various injection routes

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Comparison of γ-bungarotoxin content in different regions a...

第十四届生物毒素毒理学术大会暨第一届生物毒素——从生存适应到转化医学专题学术会议

作者： Hongfei Zhang Yun Zhang Tongyi Sun wen-hui lee Key Laboratory of Biological Medicine in Universities of Shandong Province School of Bioscience and TechnologyWeifang Medical University Key Laboratory of Bio-active Peptides of Yunnan Province/Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences Kunming Institute of Zoology

With the advancement of modern technology and the emergence of new technologies,our understanding of the composition of snake venom has increased *** recent years,studies have found that snake venom in different regions differs in composition,toxicity and *** geographical venom variation may be caused by ontogeny,geographical location or seasonal climate,and may also be related to existing diets,and the main factor causing this geographical venom variation is geographical *** investigation focused on the quantity variations of y-bungarotoxin,an important lethal factor contained in Bungarus multicinctue *** crude venoms used in this study were collected from Guangxi and Hunan provinces,*** filtration,ion-exchange chromatography,high performance liquid chromatography and mass spectrometry analysis were used to purify and identify y-bungarotoxins from different *** composition differences of ybungarotoxin in different regions were *** values of y-bungarotoxin were determined by intraperitoneal injection,intramuscular injection and tail vein injection in Kunming mice.

关键词： γ-bungarotoxin geographic variation toxicity LD50

Two potent lipopolysaccharide-binding peptides characterized from frog Bombina maxima

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Two potent lipopolysaccharide-binding peptides characterized...

第十四届生物毒素毒理学术大会暨第一届生物毒素——从生存适应到转化医学专题学术会议

作者： Caifen Guo Baiyu Zhang Jihong Shen wen-hui lee Department of Urology The First Affiliated Hospital of Kunming Medical University Key Laboratory of Bio-active Peptides of Yunnan Province/Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences Kunming Institute of Zoology

Lipopolysaccharide(LPS) is a major pathogen-associated molecular pattern molecule that can initiate lethal *** peptides(AMPs) in the skin secretions of amphibians are essential components of immune system to protect these hosts from different microbial *** present investigation,we identified two LPS-binding peptides from skin secretions of native amphibian Bombina maxima challenged with LPS *** peptides were purified by gel filtration on a superdex peptide 10/300 GL column followed by an HPLC C *** molecular weight and the purity of peptides were analyzed by an Autoflex Speed TOF/TOF mass *** primary structures of the peptides were determined by *** binding activity of peptides with LPS was determined using super streptavidin(SSA) biosensors in the Octet Red 96 *** peptides showed LPS-binding activities,low cytotoxity in vitro and protective effects on LPS-induced sepsis in *** results suggest the peptides are valuable molecules that can interact with LPS and laid the foundation for future research and development.

关键词： Antimicrobial peptides lipopolysaccharide LPS-binding activity

Amphibian pore-forming proteinβγ-CAT drives metabolite release from small extracellular vesicles through channel formation

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Zoological Research 2023年第4期44卷 739-742页

作者： Qi-Quan Wang Xin-Qiang Lan Xue-Song Wei Si-Man Xu Ling-Zhen Liu Xian-Ling Bian Lin Zeng Xiao-Long Guo Ying-Qi Guo wen-hui lee Yang Xiang Yun Zhang Human Aging Research Institute(HARI)and School of Life Science Nanchang Universityand Jiangxi Key Laboratory of Human AgingNanchangJiangxi 330031China Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences&Yunnan Province Kunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650201China Center for Excellence in Animal Evolution and Genetics Chinese Academy of SciencesKunmingYunnan 650201China Institutional Center for Shared Technologies and Facilities of the Kunming Institute of Zoology Chinese Academy of SciencesKunmingYunnan 650201China

DEAR EDITOR,Pore-forming proteins(PFPs)are widely distributed among all kingdoms of life and can oligomerize to form pores/channels in membrane *** vesicles(EVs)circulate in all biological fluids and can trigger biological responses at a distance,thus emerging as an additional means of intercellular communication through the release of cellular cargo,such as lipids,nucleic acids,metabolites,and *** date,however,the mechanism by which EV contents are released into extracellular space remains *** our previous study on toads(Bombina maxima),we identified a PFP and trefoil factor complexβγ-CAT(βγ-crystallin fused aerolysin-like protein(α-subunit)and trefoil factor(β-subunit)complex,hence namedβγ-CAT),which assembled under strict regulation in response to environmental ***,we observed that the PFPβγ-CAT colocalized with EVs in the skin of *** in *** small EVs(sEVs)isolated from *** dermal fibroblast cells and murine fibroblast cells,we found thatβγ-CAT could specifically target and oligomerize on purified EVs,rather than disrupt membrane integrity,and promote the release of different *** analysis revealed that a secretory PFP drove metabolite release from EVs through channel formation,providing novel clues for the delivery of EV content into extracellular space.

关键词： release extracellular hence