Two potent lipopolysaccharide-binding peptides characterized from frog Bombina maxima
作者单位：Department of UrologyThe First Affiliated Hospital of Kunming Medical University Key Laboratory of Bio-active Peptides of Yunnan Province/Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of SciencesKunming Institute of Zoology
会议名称：《第十四届生物毒素毒理学术大会暨第一届生物毒素——从生存适应到转化医学专题学术会议》
会议日期：2019年
学科分类：1001[医学-基础医学(可授医学、理学学位)] 10[医学]
关 键 词：Antimicrobial peptides lipopolysaccharide LPS-binding activity
摘 要：Lipopolysaccharide(LPS) is a major pathogen-associated molecular pattern molecule that can initiate lethal *** peptides(AMPs) in the skin secretions of amphibians are essential components of immune system to protect these hosts from different microbial *** present investigation,we identified two LPS-binding peptides from skin secretions of native amphibian Bombina maxima challenged with LPS *** peptides were purified by gel filtration on a superdex peptide 10/300 GL column followed by an HPLC C *** molecular weight and the purity of peptides were analyzed by an Autoflex Speed TOF/TOF mass *** primary structures of the peptides were determined by *** binding activity of peptides with LPS was determined using super streptavidin(SSA) biosensors in the Octet Red 96 *** peptides showed LPS-binding activities,low cytotoxity in vitro and protective effects on LPS-induced sepsis in *** results suggest the peptides are valuable molecules that can interact with LPS and laid the foundation for future research and development.