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[Construction of a 17 β-estradiol sensor based on a magnetic graphene oxide/aptamer separating material]

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Se pu = Chinese journal of chromatography 2025年第1期43卷 87-95页

作者： Xin-Yu Jin Le-yuan Chen Ya-Nna Liu wen-Jing Xie Han-Yong Peng State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China. University of Chinese Academy of Sciences Beijing 100049 China. School of Environment Hangzhou Institute for Advanced Study University of Chinese Academy of Sciences Hangzhou 310024 China.

17-Estradiol (E2) is a natural steroidal estrogen essential for a variety of physiological functions in organisms. However, external E2, which is renowned for its potent biological effects, is also considered to be an endocrine-disrupting compound (EDC) capable of disturbing the normal operation of the endocrine system, even at nanogram-per-liter (ng/L) concentrations. Studies have revealed that medical and livestock wastewater can be contaminated with E2, which poses potential risks to human health. Currently, the primary method for detecting E2 relies on liquid chromatography-mass spectrometry, which is limited with regard to on-site or large-scale sample testing due to instrumental constraints. Herein, we developed a magnetic graphene oxide (MGO)/aptamer separating material. The MGO was synthesized by creating a water-in-oil microemulsion at 90 ℃, an agarose hydrogel to load the FeO nanoparticles, and layered graphene oxide (GO). In contrast to conventional methods, such as chemical co-precipitation and solvothermal approaches, this method is more time-efficient and does not require high temperature or pressure. Moreover, the use of a physical encapsulation technique for enwrapping the FeO nanoparticles and layered GO eliminates the need for chemical modification. This approach reduces the use of harmful chemicals, ensures complete loading, and results in highly efficient encapsulation. The MGO was characterized using Fourier-transform infrared (FT-IR) spectroscopy and transmission electron microscopy (TEM), as well as dynamic light scattering (DLS) and Zeta potential analyses, which revealed that the FeO nanoparticles had been successfully loaded onto the GO to produce MGO particles mainly around 5 μm in size. Additionally, this study demonstrated that the aqueous MGO dispersion is highly stable. This substance was used to develop a fluorescent biosensor that uses a "turn-on" mechanism to rapidly and highly sensitively detect E2. MGO is capable of adsorbing a fl

关键词： 17-estradiol (E2) fluorescence biosensor magnetic graphene oxide (MGO) magnetic solid phase separation

Apollo: Rapidly Picking the Optimal Cloud Configurations for Big Data Analytics Using a Data-Driven Approach

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Journal of Computer Science & Technology 2021年第5期36卷 1184-1199页

作者： Yue-wen Wu yuan-Jia Xu Heng Wu Lin-Gang Su wen-Bo Zhang hua Zhong University of Chinese Academy of Sciences Beijing 100049China State Key Laboratory of Computer Science Institute of SoftwareChinese Academy of SciencesBeijing 100190China

Big data analytics applications are increasingly deployed on cloud computing infrastructures,and it is still a big challenge to pick the optimal cloud configurations in a cost-effective *** this paper,we address this problem with a high accuracy and a low *** propose Apollo,a data-driven approach that can rapidly pick the optimal cloud configurations by reusing data from similar *** first classify 12 typical workloads in BigDataBench by characterizing pairwise correlations in our offline *** a new workload comes,we run it with several small datasets to rank its key characteristics and get its similar *** on the rank,we then limit the search space of cloud configurations through a classification *** last,we leverage a hierarchical regression model to measure which cluster is more suitable and use a local search strategy to pick the optimal cloud configurations in a few extra *** evaluation on 12 typical workloads in HiBench shows that compared with state-of-the-art approaches,Apollo can improve up to 30%search accuracy,while reducing as much as 50%overhead for picking the optimal cloud configurations.

关键词： big data analytics cloud configuration data driven

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车用BUCK变换器控制策略的研究

车辆与动力技术 2017年第3期 26-31页

作者：高晓宇韩志平田德文刘华源中国北方车辆研究所北京100072

针对车载高压直流电源在发动机转速、供电负载频繁发生突变时输出直流电压产生波动的问题,提出了超前-滞后补偿策略,采用复合控制的方式对输出电压进行控制.仿真和台架试验的结果表明,该控制策略实现了在发动机转速及电源负载发生突变... 详细信息

针对车载高压直流电源在发动机转速、供电负载频繁发生突变时输出直流电压产生波动的问题,提出了超前-滞后补偿策略,采用复合控制的方式对输出电压进行控制.仿真和台架试验的结果表明,该控制策略实现了在发动机转速及电源负载发生突变时对直流电源输出电压的稳定控制.

关键词：高压直流电源 BUCK变换器超前-滞后补偿策略

Timing and hamming weight attacks on minimal cost encryption scheme

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The Journal of China Universities of Posts and Telecommunications 2009年第1期16卷 81-85页

作者： yuan Zheng WANG Wei ZHANG hua wen Qiao-yan Department of Information Security Beijing Electronic Science and Technology Institute Beijing 100070 China School of Science Beijing University of Posts and Telecommunications Beijing 100876 China The Center for Advanced Study Tsinghua University Beijing 100084 China

The timing and Hamming weight attacks on the data encryption standard （DES） cryptosystem for minimal cost encryption scheme is presented in this article. In the attack, timing information on encryption processing is used to select and collect effective plaintexts for attack. Then the collected plaintexts are utilized to infer the expanded key differences of the secret key, from which most bits of the expanded secret key are recovered. The remaining bits of the expanded secret key are deduced by the correlations between Hamming weight values of the input of the S-boxes in the first-round. Finally, from the linear relation of the encryption time and the secret key＇s Hamming weight, the entire 56 bits of the secret key are thoroughly recovered. Using the attack, the minimal cost encryption scheme can be broken with 2^23 known plaintexts and about 2^21 calculations at a success rate a 〉 99%. The attack has lower computing complexity, and the method is more effective than other previous methods.

关键词： timing and hamming weight attacks DES minimal cost encryption scheme digital right management (DRM)

Synthesis and Ferrimagnetic Behaviour of Mn Doped Rare Earth Iron Garnets

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Chemical Research in Chinese Universities 2012年第3期28卷 391-394页

作者： GUO Li yuan Lin PENG wen yuan Hong-ming FENG Shou-hua State Key Laboratory of Inorganic Synthesis and Preparative Chemistry College of ChemistryJilin UniversityChangchun 130012R R.China

Rare earth iron garnets R3Fe5-xMnxO12（R=Pr, Nd, Sm, Eu） were prepared through mild hydrothermal me- thod. The initial alkalinity of solutions was thought to play an important role in governing the content of Mn. The effect of substitution Fe^3＋ ions by Mn^3＋ ions on magnetic properties was investigated. The saturation magnetizations of Mn-doped samples are larger than that of corresponding parent compounds due to the moment of Mn^3＋ ion being smaller than that of Fe^3＋. It is clearly shown that incorporation of Mn^3＋ gives rise to significant variations in the Curie temperature. With increasing of Mn content x, Curie temperatures reduced sharply for the garnets, which could be explained by the exchange interaction between a-d Fe^3＋ being reduced in these compounds.

关键词： Hydrothermal synthesis Garnet Substitution Ferrimagnetism

Toward high-efficiency perovskite solar cells with one-dimensional oriented nanostructured electron transport materials

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Journal of Energy Chemistry 2023年第7期82卷 66-87,I0003页

作者： Yinhua Lv Bing Cai Ruihan yuan Yihui Wu Quinn Qiao wen-hua Zhang Yunnan Key Laboratory of Carbon Neutrality and Green Low-carbon Technologies School of Materials and EnergyYunnan UniversityKunming 650500YunnanChina School of Chemical Engineering Sichuan UniversityChengdu 610065SichuanChina Sichuan Research Center of New Materials Institute of Chemical MaterialsChina Academy of Engineering PhysicsChengdu 610200SichuanChina Mechanical and Aerospace Engineering Syracuse UniversitySyracuseNew York 13244USA Yunnan University and Southwest United Graduate School Kunming 650500YunnanChina

The unique advantages of one-dimensional(1D)oriented nanostructures in light-trapping and chargetransport make them competitive candidates in photovoltaic(PV)*** the emergence of perovskite solar cells(PSCs),1D nanostructured electron transport materials(ETMs)have drawn tremendous ***,the power conversion efficiencies(PCEs)of these devices have always significantly lagged behind their mesoscopic and planar ***-efficiency PSCs with 1D ETMs showing efficiency over 22%were just realized in the most recent *** yet lacks a comprehensive review covering the development of 1D ETMs and their application in *** hence timely summarize the advances in 1D ETMs-based solar cells,emphasizing on the fundamental and optimization issues of charge separation and collection ability,and their influence on PV *** sketching the classification and requirements for high-efficiency 1D nanostructured solar cells,we highlight the applicability of 1D TiO_(2)nanostructures in PSCs,including nanotubes,nanorods,nanocones,and nanopyramids,and carefully analyze how the electrostatic field affects cell *** kinds of oriented nanostructures,e.g.,ZnO and SnO_(2)ETMs,are also ***,we discuss the challenges and propose some potential strategies to further boost device *** review provides a broad range of valuable work in this fast-developing field,which we hope will stimulate research enthusiasm to push PSCs to an unprecedented level.

关键词： 1D nanostructures Perovskite solar cells Electron transport materials Electrostatic field High-efficiency

Goal-Oriented Control Systems(GOCS):From HOW to WHAT

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IEEE/CAA Journal of Automatica Sinica 2024年第4期11卷 816-819页

作者： wen-hua Chen the Department of Aeronautical and Automotive Engineering Loughborough UniversityLeicestershireLE113TUU.K.

Brief:New control theory is required to underpin safe design and deployment of future highly automated systems to deal with uncertain environments and complicated tasks,enabled by AI and other advanced ***-Oriented Control Systems offer potential to transform the control system design from currently instructing a control system how to perform a task to specifying what is to be achieved.

关键词： automated instru system

基于永磁同步电机的发动机高低压联合起动策略研究

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车辆与动力技术 2017年第1期 29-32页

作者：高晓宇韩志平田德文刘华源中国北方车辆研究所北京100072

针对低温环境下发动机起动困难的问题,根据发动机起动过程中不同转速下的起动力矩需求,基于永磁同步电机原理及最大扭矩电流比控制方法,采用曲线拟合,确定了电机起动中电流的控制方案,实现了高低压联合起动时,最低能耗下发动机的可靠起... 详细信息

针对低温环境下发动机起动困难的问题,根据发动机起动过程中不同转速下的起动力矩需求,基于永磁同步电机原理及最大扭矩电流比控制方法,采用曲线拟合,确定了电机起动中电流的控制方案,实现了高低压联合起动时,最低能耗下发动机的可靠起动,并通过起动系统样机台架性能测试,验证了联合起动策略的有效性.

关键词：永磁同步电机最大转矩电流比控制联合起动控制策略

Gene Therapy Activates Retinal Pigment Epithelium Cell Proliferation for Age-related Macular Degeneration in a Mouse Model

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Current Medical Science 2023年第2期43卷 384-392页

作者： Yun yuan wen KONG Xiao-mei LIU Guo-hua SHI School of Biomedical Engineering(Suzhou) Division of Life Sciences and MedicineUniversity of Science and Technology of ChinaSuzhou215000China Suzhou Institute of Biomedical Engineering and Technology Chinese Academy of ScienceSuzhou215000China

Objective Age-related macular degeneration(AMD)is a degenerative retinal *** degeneration or death of retinal pigment epithelium(RPE)cells is implicated in the pathogenesis of *** study aimed to activate the proliferation of RPE cells in vivo by using an adeno-associated virus(AAV)vector encodingβ-catenin to treat AMD in a mouse *** Mice were intravitreally injected with AAV2/8-Y733F-VMD2-β-catenin for 2 or 4 weeks,andβ-catenin expression was measured using immunofluorescence staining,real-time quantitative reverse transcription polymerase chain reaction(PCR),and Western *** function ofβ-catenin was determined using retinal flat mounts and laser-induced damage ***,the safety of AAV2/8-Y733F-VMD2-β-catenin was evaluated by multiple intravitreal *** AAV2/8-Y733F-VMD2-β-catenin induced the expression ofβ-catenin in RPE *** activated the proliferation of RPE cells and increased cyclin D1 *** was beneficial to the recovery of laser-induced damage by activating the proliferation of RPE ***,it could induce apoptosis of RPE cells by increasing the expression of Trp53,Bax and caspase3 while decreasing the expression of *** AAV2/8-Y733F-VMD2-β-catenin increasedβ-catenin expression in RPE cells,activated RPE cell proliferation,and helped mice heal from laser-induced eye ***,it could induce the apoptosis of RPE ***,it may be a safe approach for AMD treatment.

关键词： gene therapy adeno-associated virus age-related macular degeneration retinal pigment epithelium cells β-catenin