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Soybean(Glycine max)rhizosphere organic phosphorus recycling relies on acid phosphatase activity and specific phosphorusmineralizing-related bacteria in phosphate deficient acidic soils

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Journal of Integrative Agriculture 2024年第5期23卷 1685-1702页

作者： Qianqian Chen Qian Zhao Baoxing Xie Xing lu Qi Guo Guoxuan Liu Ming Zhou Jihui Tian weiguo lu Kang Chen Jiang Tian Cuiyue Liang Root Biology Center State Key Laboratory for Conservation and Utilization of Subtropical Agro-BioresourcesCollege of Natural Resources and EnvironmentSouth China Agricultural UniversityGuangzhou 510642China College of Natural Resources and Environment South China Agricultural UniversityGuangzhou 510642China Henan Academy of Crops Molecular Breeding Henan Academy of Agricultural SciencesZhengzhou 450002China

Bacteria play critical roles in regulating soil phosphorus(P) cycling. The effects of interactions between crops and soil P-availability on bacterial communities and the feedback regulation of soil P cycling by the bacterial community modifications are poorly understood. Here, six soybean(Glycine max) genotypes with differences in P efficiency were cultivated in acidic soils with long-term sufficient or deficient P-fertilizer treatments. The acid phosphatase(AcP) activities, organic-P concentrations and associated bacterial community compositions were determined in bulk and rhizosphere soils. The results showed that both soybean plant P content and the soil AcP activity were negatively correlated with soil organic-P concentration in P-deficient acidic soils. Soil P-availability affected the ɑ-diversity of bacteria in both bulk and rhizosphere soils. However, soybean had a stronger effect on the bacterial community composition, as reflected by the similar biomarker bacteria in the rhizosphere soils in both P-treatments. The relative abundance of biomarker bacteria Proteobacteria was strongly correlated with soil organic-P concentration and AcP activity in low-P treatments. Further high-throughput sequencing of the phoC gene revealed an obvious shift in Proteobacteria groups between bulk soils and rhizosphere soils, which was emphasized by the higher relative abundances of Cupriavidus and Klebsiella, and lower relative abundance of Xanthomonas in rhizosphere soils. Among them, Cupriavidus was the dominant phoC bacterial genus, and it was negatively correlated with the soil organic-P concentration. These findings suggest that soybean growth relies on organic-P mineralization in P-deficient acidic soils, which might be partially achieved by recruiting specific phoCharboring bacteria, such as Cupriavidus.

关键词： organic phosphorus acid phosphatase soybean bacterial community phoC-harboring bacteria rhizosphere

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RAD51B-AS1 promotes the malignant biological behavior of ovarian cancer through upregulation of RAD51B

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2024年第7期25卷 581-593页

作者： Xinyi WEI Conghui WANG Sangsang TANG Qian YANG Zhangjin SHEN Jiawei ZHU Xiaodong CHENG Xinyu WANG Xing XIE Junfen XU weiguo lu Women's Reproductive Health Laboratory of Zhejiang Province Women�s HospitalZhejiang University School of MedicineHangzhou 310006China Department of Gynecologic Oncology Women�s HospitalZhejiang University School of MedicineHangzhou 310006China Cancer Center Zhejiang UniversityHangzhou 310058China Department of Obstetrics and Gynecology the First Affiliated HospitalZhejiang University School of MedicineHangzhou 310003China Zhejiang Provincial Clinical Research Center for Obstetrics and Gynecology Hangzhou 310006China

Long non-coding RNAs(lncRNAs)play an indispensable role in the occurrence and development of ovarian cancer(OC).However,the potential involvement of lncRNAs in the progression of OC is largely *** investigate the detailed roles and mechanisms of RAD51 homolog B-antisense 1(RAD51B-AS1),a novel lncRNA in OC,reverse transcription-quantitative polymerase chain reaction(RT-qPCR)was performed to verify the expression of *** proliferation,metastasis,and apoptosis were detected using the cell counting kit-8(CCK-8),colony-formation,transwell,and flow cytometry *** xenograft models were established for the detection of *** results revealed that RAD51B-AS1 was significantly upregulated in a highly metastatic human OC cell line and OC ***51B-AS1 significantly increased the proliferation and metastasis of OC cells and enhanced their resistance to *** prediction analysis revealed that the only target gene of RAD51B-AS1 was *** gene function experiments revealed that RAD51B exerts the same biological effects as *** experiments demonstrated that the malignant biological behaviors promoted by RAD51B-AS1 overexpression were partially or completely reversed by RAD51B silencing in vitro and in ***,RAD51B-AS1 promotes the malignant biological behaviors of OC and activates the protein kinase B(Akt)/B cell lymphoma protein-2(Bcl-2)signaling pathway,and these effects may be associated with the positive regulation of RAD51B ***51B-AS1 is expected to serve as a novel molecular biomarker for the diagnosis and prediction of poor prognosis in OC,and as a potential therapeutic target for disease management.

关键词： Ovarian cancer(OC) Long non-coding RNA(LncRNA) Metastasis Anoikis

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Identifcation and Characterization of a New Geminivirus from Soybean Plants and Determination of V2 as a Pathogenicity Factor and Silencing Suppressor

Identifcation and Characterization of a New Geminivirus from...

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中国植物病理学会2024年学术年会

作者： LI Qinglun ZHANG Yuyang lu weiguo HAN Xiaoyu YANG Lingling SHI Yajuan LI Honglian CHEN Linlin YANG Xue SHI Yan College of Plant Protection Henan Agricultural University Institute of Crops Molecular Breeding Henan Academy of Agricultural Sciences/National Centre for Plant Breeding

Soybean is one of the four major crops in *** occurrence of viruses in soybean causes significant economic *** this study,the soybean leaves from stay-green plants showing crinkle were collected for metatranscriptomic sequencing.A novel geminivirus,tentatively named soybean geminivirus A(SGVA),was identified in soybean stay-green *** analysis of the fulllength SGVA genome revealed a genome of 2 762 nucleotides that contain six open reading *** revealed that SGVA was located adjacent to the clade of begomoviruses in both the full genome-based and C1-based phylogenetic tree,while in the CP-based phylogenetic tree,SGVA was located adjacent to the clade of *** was proposed as a new recombinant *** clone of SGVA was *** systemic symptoms of curly leaves were observed at 11 dpi in Nicotiana benthamiana plants and severe dwarfism was observed after 3 weeks post *** of the SGVA encoded V2 and C1 proteins through a potato virus X(PVX) vector caused severe symptoms in *** V2 protein inhibited local RNA silencing in co-infiltration assays in GFP transgenic 16 C *** *** study revealed mild symptoms in *** plants inoculated with SGVA-ZZ V2-STOP and SGVA-ZZ V2-3738 AA *** the relative viral DNA and CP protein accumulation levels significantly decreased when compared with SGVA-inoculated *** work identified a new geminivirus in soybean stay-green plants and determined V2 as a pathogenicity factor and silencing suppressor.

关键词： Genetic variability Pathogenicity RNA silencing suppressor Soybean geminivirus A(SGVA) V2 protein

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无电流传感复合前馈控制无桥功率因数校正变换器

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电工技术学报 2024年第14期39卷 4445-4455页

作者：卢伟国胡凌鹤罗海魁白月张婷婷输配电装备及系统安全与新技术国家重点实验室(重庆大学) 重庆400044

有源功率因数校正(APFC)是AC-DC变换器的重要组成部分,其目的是改善输入侧电能质量,提高系统功率因数。传统的有源电流控制功率因数校正(PFC)变换器需要准确地提取升压电感电流,而双二极管式无桥PFC变换器升压电感位于整流部分之前,电... 详细信息

有源功率因数校正(APFC)是AC-DC变换器的重要组成部分,其目的是改善输入侧电能质量,提高系统功率因数。传统的有源电流控制功率因数校正(PFC)变换器需要准确地提取升压电感电流,而双二极管式无桥PFC变换器升压电感位于整流部分之前,电感电流不再是单一方向流动,这使得其电流采样电路相比更复杂。为此该文提出一种基于观测器的无电流传感方案。方案中针对占空比误差的不确定影响修正了无桥PFC电流观测器算法,实现了占空比误差自适应的电流观测器,进一步基于所提观测器实现对负载信息的观测,设计出无电流传感的复合前馈控制方案,运用负载观测信息改进电流内环参考形式,同时利用输出电压纹波观测值进行2次谐波前馈补偿,最终实现无电流传感控制条件下变换器高动态响应设计。该文搭建了225 W单相双二极管式无桥PFC的实验样机,实验结果表明,在开关频率100 kHz时电流观测精度达到96.75%,所提控制方案输入电流的总谐波畸变率(THD)含量约为5%,功率因数(PF)约为99.85%,半载动态响应时间10 ms,相比于传统PI控制无桥PFC变换器提高了约90%。

关键词：无桥PFC变换器电流观测器前馈控制动态响应占空比自适应

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The cGAS-STING pathway:a therapeutic target in chromosomally unstable cancers

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Signal Transduction and Targeted Therapy 2023年第2期8卷 338-340页

作者： Haiyan Yan weiguo lu Fangwei Wang Key Laboratory of Novel Targets and Drug Study for Neural Repair of Zhejiang Province School of MedicineHangzhou City University310015HangzhouChina Women’s Hospital Zhejiang University School of Medicine310006HangzhouChina Cancer Center Zhejiang University310058HangzhouChina The MOE Key Laboratory of Biosystems Homeostasis&Protection and Zhejiang Provincial Key Laboratory of Cancer Molecular Cell Biology Life Sciences InstituteZhejiang University310058HangzhouChina

In a recent study published in Nature,Hong et *** that the cyclic GMP-AMP synthase(cGAS)-stimulator of interferon genes(STING)innate immune pathway is critically required for the IL-6-dependent survival of chromosomally unstable cancer cells,1 implying that chronic inflammation can be therapeutically targeted to kill cancers displaying chromosomal instability(CIN).

关键词： unstable chromosomal therapeutic

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Effects of tacrolimus (FK506) on sciatic nerve regeneration in rats

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Neural Regeneration Research 2009年第5期4卷 333-338页

作者： weiguo Zhang Decheng lu Shouyu Wang Chongyang Fu Department of Orthopedic Surgery First Affiliated Hospital Dalian Medical University Dalian 116011 Liaoning Province China

BACKGROUND： Tacrolimus （FK506） protects peripheral nerves located in damaged regions by inhibiting T lymphocyte proliferation and activation. OBJECTIVE： To evaluate the effect of FK506 on promoting regeneration of rat sciatic nerve. DESIGN, TIME AND SETTING： A randomized, controlled, animal study was performed at the Laboratory of the Department of Orthopedic Surgery, Dalian Medical University, China, from September 2007 to September 2008. MATERIALS： A total of 60 adult, male, Sprague-Dawley rats were equally and randomly divided into model, local administration and systemic administration groups. All rats received a neurotomy of bilateral sciatic nerves to establish models of nerve regeneration chambers. The powder and injection of FK506 were supplied by Fujisawa Pharmaceutical, Japan. METHODS： The regeneration chambers of the model group were infused with 0.2 mL saline. The systemic group were injected with 0.2 mL saline, followed by daily subcutaneous injections of FK506 （1 mg/kg）, for 14 days. The local administration group was infused with 0.2 mL FK506 （1 μg/mL）. MAIN OUTCOME MEASURES： Local immune response was observed using hematoxylin-eosin staining. Myelinated nerve fiber number, myelin sheath and nerve fiber thickness were observed using toluidine blue staining. Wet weight of gastrocnemius was evaluated. Compound muscle action potential amplitude, latency, and conduction time were recorded, and motor nerve conduction velocity was calculated using electrophysiology. RESULTS： The total number of myelinated nerve fibers in the local and systemic administration groups was significantly higher than in the model group. The density of myelinated nerve fibers, myelin sheath thickness and mean axon diameter were significantly increased in the systemic administration group compared with the model group （P 〈 0.05）. Lymphocyte infiltration was decreased in the local and systemic administration groups compared with the model group. The wet weight of rat gastrocnemius i

关键词： tacrolimus (FK506) neural regeneration sciatic nerve

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低扭矩环锭纺对羊毛纱性能的影响

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毛纺科技 2024年第5期52卷 1-5页

作者：郑光明华新忠陆卫国李豪郁崇文东华大学纺织学院上海201620 浙江新澳纺织股份有限公司浙江嘉兴314000

为了实现羊毛纱低捻高强目标,提高织物质量,探究低扭矩环锭纺纱工艺对不同捻系数羊毛纱性能和织物纬斜情况的影响,采用低扭矩环锭纺纱工艺纺制不同捻系数的25 tex羊毛纱,并与25 tex常规环锭纺羊毛纱的断裂强度、断裂伸长率、毛羽指数和... 详细信息

为了实现羊毛纱低捻高强目标,提高织物质量,探究低扭矩环锭纺纱工艺对不同捻系数羊毛纱性能和织物纬斜情况的影响,采用低扭矩环锭纺纱工艺纺制不同捻系数的25 tex羊毛纱,并与25 tex常规环锭纺羊毛纱的断裂强度、断裂伸长率、毛羽指数和条干均匀度进行对比。结果表明:羊毛纱线密度为25 tex时,相同捻系数下,低扭矩环锭纺纱工艺羊毛纱的断裂强度较常规环锭纺平均提高约10%;断裂伸长率较常规环锭纺平均提高15%左右;长毛羽(3~9 mm)指数下降趋势明显,降低了织物的起毛起球;短毛羽(1~2 mm)指数上升趋势明显,使织物触感更加丰满和柔软;条干均匀度较常规环锭纺显著降低;低扭矩环锭纺羊毛纱所织针织小样较常规环锭纺纬斜率降低15%左右,为羊毛纱及针织物的开发提供了新的思路和方法。

关键词：低扭矩纱环锭纺羊毛纱强伸性能毛羽指数纬斜率

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Verification and fine-mapping of QTLs conferring days to flowering in soybean using residual heterozygous lines

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Chinese Science Bulletin 2010年第6期55卷 499-508页

作者： SU ChengFu lu weiguo ZHAO TuanJie GAI JunYi Soybean Research Institute Nanjing Agricultural University National Center for Soybean Improvement National Key Laboratory for Crop Genetics and Germplasm Enhancement Nanjing 210095 China Institute of Cotton and Oil Crops Henan Academy of Agricultural Sciences Zhengzhou 450002 China

The results of QTL mapping based on a primary mapping population should be further verified and refined for its real utilization in marker-assisted selection or map-based *** primary mapping population contains 114 BC1F1 plants of the backcross between Essex (maturity group V,MG V) as the donor parent and ZDD2315 (MG II) as the recurrent *** this study,a genetic linkage map with 250 SSR markers spanning a total length of 2963.5 cM on 25 linkage groups (LG) was constructed using software *** kinds of genetic statistical models of 4 softwares,*** Cartographer Version 2.5,IciMapping Version 2.0,MapQTL Version 5.0 and QTLnetwork Version 2.0,were used to map QTLs conferring days to flowering of the BC1F3 *** QTLs were mapped on 6 different linkage groups (LG).Of those,6 QTLs were detected by at least two different genetic statistical models,while the other three were detected by only one *** the three QTLs,Flwdt7 was mapped between Sat_213 and Satt643 on LG C2 with only 11.0% contribution *** confirmation of Flwdt7,5 RHL populations were developed through selfing eight BC1F5 plants heterozygous at seven markers around the *** RHL populations with the same segregating loci were bulked and used to construct a secondary linkage map of the specific segment using software JoinMap *** genetic distances among the markers on the specific segment became shorter than those of the whole genome *** the secondary map,Flwdt7 was mapped between Satt277 and Satt489,next to its primary interval Sat_213-Satt643,with distance 1.40 cM to Satt277 and 0.45 cM to Satt489,confidence interval narrowed to 2.7 cM,and contribution rate increased to 36.8%.The results were confirmed with significance analysis among marker genotypes on individual loci and comparison analysis of target marker intervals among near isogenic lines (plants).Thus the strategy by using residual heterozygous lines for QTL fine-mapping on target segments ba

关键词：大豆回交线 RHL (剩余异质接合的线) SSR (简单顺序重复) 标记 flowering 日期 QTL 好印射

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An ASIC design for LHAASO

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Science China(Physics,Mechanics & Astronomy) 2011年第10期54卷 1911-1914页

作者： YAN XiongBo WANG Zheng CHANG JinFan WEI Wei lu weiguo State Key Laboratory of Particle Detection and Electronics Institute of High Energy Physics Chinese Academy of Sciences Beifing 100049 China Graduate University of Chinese Academy of Sciences Beijing 100049 China

The ASIC is widely used in the field of high energy physics. Astroparticle experiments benefit from high integration, smallsize and low power consumption. In this paper an analog chip named ARCHGARD (Analog Readout Chip for High energyGamma Ray Detector) for high energy physics experiment is introduced. ARCHGARD is a readout chip, in chartered 0.35 mSiGe technology, for photomultipliers (PMT) array readout. The chip is designed for the Large High Altitude Air Shower Ob-servatory (LHAASO) project. The ASIC integrates 16 independent and auto-triggered channels with variable gain and variableshaping time. It provides analog output for charge measurement, which is performed from 1 up to 3000 photo-electrons (p.e.).The integral nonlinearity of the whole input range is less than 1% and the equivalent input noise is less than 1/10 p.e. Eachchannel has a trigger output for time measurement.

关键词： LHAASO analog ASIC PMT readout large dynamic range low noise good linearity

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Deep Learning for Covert Communication

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China Communications 2024年第9期21卷 40-59页

作者： Shen weiguo Chen Jiepeng Zheng Shilian Zhang luxin Pei Zhangbin lu Weidang Yang Xiaoniu Innovation Studio of Academician Yang National Key Laboratory of Electromagnetic Space SecurityJiaxing 314033China College of Information Engineering Zhejiang University of TechnologyHangzhou 310023China School of Information Science and Technology Fudan UniversityShanghai 200438China

In recent years,deep learning has been gradually used in communication physical layer receivers and has achieved excellent *** this paper,we employ deep learning to establish covert communication systems,enabling the transmission of signals through high-power signals present in the prevailing environment while maintaining covertness,and propose a convolutional neural network(CNN)based model for covert communication receivers,namely Deep *** model leverages CNN to execute the signal separation and recovery tasks commonly performed by traditional *** enables the direct recovery of covert information from the received *** simulation results show that the proposed Deep CCR exhibits significant advantages in bit error rate(BER)compared to traditional receivers in the face of noise and multipath *** verify the covert performance of the covert method proposed in this paper using the maximum-minimum eigenvalue ratio-based method and the frequency domain entropy-based *** results indicate that this method has excellent covert *** also evaluate the mutual influence between covert signals and opportunity signals,indicating that using opportunity signals as cover can cause certain performance losses to covert *** the interference-tosignal power ratio(ISR)is large,the impact of covert signals on opportunity signals is minimal.

关键词： convolutional neural network covert communication deep learning

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