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Toward understanding genetic mechanisms of complex traits in rice

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Journal of Genetics and Genomics 2010年第10期37卷 653-666页

作者： wei Hao hong-Xuan Li National Key Laboratory of Plant Molecular Genetics Shanghai Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China

Rice is the primary carbohydrate staple cereal feeding the world population. Many genes, known as quantitative trait loci （QTLs）, con- trol most of the agronomically important traits in rice. The identification of QTLs controlling agricultural traits is vital to increase yield and meet the needs of the increasing human population, but the progress met with challenges due to complex QTL inheritance. To date, many QTLs have been detected in rice, including those responsible for yield and grain quality; salt, drought and submergence tolerance; disease and insect resistance; and nutrient utilization efficiency. Map-based cloning techniques have enabled scientists to successfully fine map and clone approximately seventeen QTLs for several traits. Additional in-depth functional analyses and characterizations of these genes will provide valuable assistance in rice molecular breeding.

关键词： rice quantitative trait loci (QTLs) mapping populations marker-assisted selection (MAS) map-based cloning

Paradigms and Paradox in the Ethylene Signaling Pathway and Interaction Network

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Molecular Plant 2011年第4期4卷 626-634页

作者： Qiong Zhao hong-wei Guo State Key Laboratory of Protein and Plant Gene Research College of Life Sciences Peking University Beijing 100871 China

Phytohormone ethylene plays pivotal roles in plant response to developmental and environmental signals. During the past few years, the emerging evidence has led us to a new understanding of the signaling mechanisms and regulatory networks of the ethylene action. In this review, we focus on the major advances made in the past three years, particularly the findings leading to new paradigms and the observations under debate. With the recent demonstration of the regulation of the protein stability of numerous key signaling components including EIN3, ELL1, EIN2, ETR2, EBFI/EBF2, and ETPI/ETP2, we highlight proteasome-dependent protein degradation as an essential regulatory mechanism that is widely adopted in the ethylene signaling pathway. We also discuss the implication of the negative feedback mechanism in the ethylene signaling pathway in light of ethylene-induced ETR2 and EBF2 gene expression. Meanwhile, we summarize the controversy on the involvement of MKK9-MPK3/6 cascade in the ethylene signaling versus biosynthesis pathway, and discuss the possible role of this MAPK module in the ethylene action. Finally, we describe the complex interactions between ethylene and other signaling pathways including auxin, light, and plant innate immunity, and propose that EIN3/ EIL1 act as a convergence point in the ethylene-initiated signaling network.

关键词： Ethylene signaling MAPK protein turnover negative feedback ETP1/2 interplay.

Tidal current turbine based on hydraulic transmission system

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2011年第7期12卷 511-518页

作者： hong-wei LIU wei LI Yong-gang LIN Shun MA State Key Laboratory of Fluid Power Transmission and Control Zhejiang University Hangzhou 310027 China

Tidal current turbines(TCTs) are newly developed electricity generating *** at the stabilization of the power output of TCTs,this paper introduces the hydraulic transmission technologies into *** hydrodynamics of the turbine was analyzed at first and its power output characteristics were predicted.A hydraulic power transmission system and a hydraulic pitch-controlled system were *** related simulations were ***,a TCT prototype was manufactured and tested in the *** test results have confirmed the correctness of the current design and availability of installation of the hydraulic system in TCTs.

关键词： Tidal current turbines(TCTs) Hydraulic power transmission Hydraulic pitch-controlled system Workshop test

Enhancement of Strength–Ductility Balance of the Laser Melting Deposited 12CrNi2 Alloy Steel Via Multi-step Quenching Treatment

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Acta Metallurgica Sinica(English Letters) 2021年第9期34卷 1234-1244页

作者： wei Zhang Zhi-hong Dong hong-wei Kang Chen Yang Yu-Jiang Xie Mohamad Ebrahimnia Xiao Peng School of Materials Science and Engineering University of Science and Technology of ChinaHefei 230000China Shi-Changxu Innovation Center for Advanced Materials Institute of Metal ResearchChinese Academy of SciencesShenyang 110016China Zhejiang Handsome Intelligent Remanufacturing Technology Co.Ltd jiaxing 314000China School of Materials Science and Engineering Nanchang Hangkong UniversityNanchang 330063China

A ferrite–austenite 12CrNi2 alloy steel additively manufactured by laser melting deposition(LMD) was heat treated by direct quenching(DQ) and tempering inter-critical quenching(TIQ) at 800 ℃ for enhancing its strength–ductility balance. Both heat-treated alloy steels have the martensite–ferrite dual-phase(DP) microstructures. The volume fractions of martensite in the two treated alloy steels are nearly similar(~ 85 vol%), while the sizes of the prior austenitic grain for martensite are different. The martensite-dominated DP microstructure resulted in an obvious improvement in strength–ductility balance of the alloy steel. Compared with the DQ treatment, the multi-step TIQ treatment caused the strength–ductility balance of the alloy steel to be enhanced due to its higher total elongation. The better ductility of the TIQ-treated alloy steel can be attributed to the optimization of the microstructure. The preferred orientation of ferritic grain in the as-deposited alloy steel which was adverse to plastic deformation through dislocation slip was eliminated via the multi-step TIQ treatment. Moreover, the TIQ treatment promoted the formation of finer-grained martensite with larger areas of grain boundaries and twinning boundaries which resulted in the enhancement of the coordinated deformability of the martensite with the ferrite.

关键词： Laser melting deposition Alloy steel Dual-phase microstructure Mechanical property Work-hardening rate

Characteristics of Soybean Urease Mineralized Calcium Carbonate and Repair of Concrete Surface Damage

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Journal of Wuhan University of Technology(Materials Science) 2021年第1期36卷 70-76页

作者： FAN Yanan DU hongxiu wei hong College of Civil Engineering Taiyuan University of TechnologyTaiyuan 030024China

The C60 concrete blocks with surface crack damage under high temperature environment were soaked by adding appropriate amount of soybean urease into the CO(NH_(2))_(2)-CaCl_(2) solution,the soybean urease mineralized calcium carbonate were characterized,and the effect of repairing concrete surface crack damage were evaluated by the surface sedimentation of C60 concrete blocks in the *** experimental results showed that the activity of soybean powder was statistically significant,and its productivity of urease was comparable with that of urease-producing *** immersion in a soybean solution,a layer of complete and continuous white sediment covered the concrete *** cracks on the concrete surface were completely shielded,and the rising temperature on infrared thermal image of the concrete after repair was lower than ***,through analysis by SEM,EDS,and XRD,the products formed after repair were found to be calcite-type CaCO_(3) with high purity,and the crystals exhibited different morphological *** above results indicate that soybean urease can regulate and induce the formation of calcium carbonate,and the precipitate is innocuous and harmless,suitable for a new type of concrete crack repair material.

关键词： soybean urease concrete calcium carbonate remediation damage

Super-resolution GANs for upscaling unplanned urban settlements from remote sensing satellite imagery - the case of Chinese urban village detection

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International Journal of Digital Earth 2023年第1期16卷 2623-2643页

作者： Alessandro Crivellari hong wei Chunzhu wei Yuhui Shi Department of Computer Science and Engineering SouthernUniversity of Science and TechnologyShenzhenPeople’s Republic of China Ministry of Education Ecological Field Station for East Asian Migratory Birds Department of Earth System ScienceInstitute for Global Change StudiesTsinghua UniversityBeijingPeople’s Republic of China School of Geography and Planning Sun Yat-sen UniversityGuangzhouPeople’s Republic of China Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai) ZhuhaiPeople’s Republic of China

The semantic segmentation of informal urban settlements represents an essential contribution towards renovation strategies and reconstruction *** this context,however,a big challenge remains unsolved when dealing with incomplete data acquisitions from multiple sensing devices,especially when study areas are depicted by images of different *** practice,traditional methodologies are directed to downgrade the higher-resolution data to the lowest-resolution measure,to define an overall homogeneous dataset,which is however ineffective in downstream segmentation activities of such crowded unplanned urban *** this purpose,we hereby tackle the problem in the opposite direction,namely upscaling the lower-resolution data to the highest-resolution measure,contributing to assess the use of cutting-edge super-resolution generative adversarial network(SR-GAN)*** experimental novelty targets the particular case involving the automatic detection of‘urban villages’,sign of the quick transformation of Chinese urban *** aligning image resolutions from two different data sources(Gaofen-2 and Sentinel-2 data),we evaluated the degree of improvement with regard to pixel-based landcover segmentation,achieving,on a 1 m resolution target,classification accuracies up to 83%,67%and 56%for 4x,8x,and 10x resolution upgrades respectively,disclosing the advantages of artificially-upscaled images for segmenting detailed characteristics of informal settlements.

关键词： SR-GAN resolution upscaling informal settlements urban villages cross-sensor analytics

Regulation of JAK/STAT signal pathway by miR-21 in the pathogenesis of juvenile idiopathic arthritis

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World Journal of Pediatrics 2020年第5期16卷 502-513页

作者： hong-wei Li Hua-Song Zeng Department of Pediatric Allergy Immunology and Rheumatology Guangzhou Women and Children Medical CenterGuangzhou 510623China Department of Pediatric Allergy Immunology and RheumatologyGuangzhou Women and Children's Medical CenterGuangzhou Medical University9 Jinsui RoadGuangzhou 510175China

Background Overexpression of the components of the Janus kinase/signal transducer and activator of transcription(JAK/STAT)signalling pathway is the key factor of the pathogenic mechanisms underlying systemic juvenile idiopathic arthritis(sJIA).The study aims to investigate the association between miR-21 and the JAK/STAT signal pathway in *** Total RNA was extracted from peripheral blood mononuclear cells(PBMCs)in active JIA *** relative expressions of miR-21,STAT3 and suppressor of cytokine signalling 3 in PBMCs were measured by real-time polymerase chain reaction and their expressions were measured by western blotting and dual-luciferase reported *** arthritis fibroblast-like synovial cell(RASF)was stimulated to become to osteoclasts using macrophage colony-stimulating factor(M-CSF)and factors that can impact on their differentiation ability were identified through the transfection of *** expression of STAT3/p-STAT3 was measured by western blot,and the levels of interleukin(IL)-17A,p65,matrix metalloproteinases(MMP)-3,MMP-4 and receptor activator of nuclear factor-κd3 after the LV3-miR-21 transfection were tested by enzyme-linked immunosorbent ***,the miR-21 targeted STAT3 gene was detected by the dualluciferase reported *** The expression of miR-21 was significantly lower in JIA patients than in healthy control(P<0.05).The level of STAT3 was increased in PBMCs of JIA group compared with control group(P<0.05).Furthermore,the expression levels of miR-21 in sJIA and polyarticular JIA groups were negatively correlated with STAT3(r=-0.5854/r=-0.6134,P<0.05).The expression of STAT3 changed little in PBMCS after the stimulation of IL-6 and not in RASFs with transfection of *** expression of p-STAT3 decreased after the stimulation of IL-6 in RASFs transfected by LV3-miR-21(P<0.05).RASFs were induced into osteoclasts using *** number of osteoclasts as determined by tartrate-resistant acid phospha

关键词： JAK/STAT Juvenile idiopathic arthritis MiR-21 Signal transducer and activator of transcription 3 (STAT3)/p-STAT3

Effects of N/Z on Spin Distribution of Evaporation Residue Cross Section as a Probe of Nuclear Dissipation

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Communications in Theoretical Physics 2008年第9期50卷 729-732页

作者： YE wei YANG hong-wei CHEN Na Department of Physics Southeast University Nanjing 210096 China Depaxtment of Applied Mathematics Nanjing University of Finance ＆ Economics Nanjing 210046 China

The spin distribution of the evaporation residue cross section of nuclei ^194pb, ^200Pb, ^206Pb, and ^200 Os are calculated via a Langevin equation coupled with a statistical decay model. It is shown that with increasing the neutronto-proton ratio （N/Z） of the system, the sensitivity of the significantly. Moreover, for ^200Os this spin distribution is no spin distribution to the nuclear dissipation is decreased longer sensitive to the nuclear dissipation. These results suggest that to obtain a more accurate pre-saddle viscosity coefficient through the measurement of the evaporation residue spin distribution, it is best to yield those compound systems with low N/Z.

关键词： evaporation residue spin distribution N/Z effect nuclear dissipation Langevin equation

The rice PLATZ protein SHORT GRAIN6 determines grain size by regulating spikelet hull cell division

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Journal of Integrative Plant Biology 2020年第6期62卷 847-864页

作者： Shi-Rong Zhou hong-wei Xue National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesShanghai Institute of Plant Physiology and Ecologythe Chinese Academy of SciencesShanghai 200032China State Key Laboratory of Crop Genetics and Germplasm Enhancement Jiangsu Plant Gene Engineering Research CenterNanjing Agricultural UniversityNanjing 210095China School of Agriculture and Biology Shanghai Jiao Tong UniversityShanghai 200240China

Grain size is a major determinant of cereal grain yields;however,the relevant regulatory mechanisms controlling this trait have not been fully *** rice(Oryza sativa)mutant short grain6(sg6)was identified based on its reduced grain length and ***,we functionally characterized the role of SG6 in determining grain size through the regulation of spikelet hull cell ***6 encodes a previously uncharacterized plant ATrich sequence and zinc-binding(PLATZ)protein that is ubiquitously localized throughout the cell and is preferentially expressed in the early developing panicles but not in the *** overexpression of SG6 resulted in significantly larger and heavier grains,as well as increased plant heights,which is consistent with its elevated spikelet hull cell division *** two-hybrid analyses revealed that SG6 interacts with the core cell cycle machinery DP protein and several other putative cell division regulators,consistent with our transcriptomic analysis,which showed that SG6 activates the expression of many DNA replication and cell-cycle-related *** results confirm the crucial role of SG6 in determining grain size by regulating spikelet hull cell division and provide clues for understanding the functions of PLATZ family proteins and the network regulating cereal grain size.

关键词： sativa) fir cereal