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Evolutionary relationship of 5′-untranslated regions among Thai dengue-3 viruses,Bangkok isolates,during 24 year-evolution

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Asian Pacific Journal of Tropical Medicine 2015年第3期8卷 176-184页

作者： watcharee Attatippaholkun Panyupa Pankhong Ananda Nisalak Siripen Kalayanarooj Department of Clinical Chemistry Faculty of Medical TechnologyMahidol University Department of Virology US Army Medical ComponentArmed Forces Research Institute of Medical Sciences Queen Sirikit National Institute of Child Health

Objective:To study evolutionary relationship of the 5'untranslated regions(5'UTRs) in low passage dengue3 viruses(DEN3) isolated from hospitalized children with different clinical manifestations in Bangkok during 24 year-evolution(1977-2000) comparing to the DEN3prototype(H87).Methods:The 5'UTRs of these Thai DEN3 and the H87 prototype were amplified by RT-PCR and *** multiple sequence alignments were done by Codon Code Aligner v 4.0.4 software and their RNA secondary structures were predicted by MFOLD *** of five Thai DEN3 candidates comparing to the 1187 prototype were done in human(HepG2) and the mosquito(C6/36) cell ***:Among these Thai DEN3,the completely identical sequences of their first 89 nucleotides,their high-order secondary structure of 5'UTRs and three SNPs including the predominant C90 T,and two minor SNPs including A109 G and A112 G were *** C90 T of Thai *** isolates was shown predominantly before *** Thai DEN3 candidates with the predominant C90 T were shown to replicate in human(HepG2) and the mosquito(C6/36) cell lines better than the H87 ***,their highly conserved sequences as well as SNPs of the 5'UTR did not appear to correlate with their disease severity in ***:Our findings highlighted evolutionary relationship of the completely identical 89 nucleotide sequence,the high-order secondary structure and the predominant C90 T of the 5'UTR of these Thai DEN3 during 24 year-evolution further suggesting to be their genetic markers and magic targets for future research on antiviral therapy as well as vaccine approaches of Thai DEN3.

关键词： Thai dengue-3 viruses Evolutionary relationship 5’untranslated regions 24 Year-evolution

Performance and correlation of QuantiFERON-TB Gold, *** and tuberculin skin test in young children with or exposed to tuberculosis

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Asian Pacific Journal of Tropical Medicine 2020年第9期13卷 423-425页

作者： Keswadee Lapphra Paninun Srinuchasart Sansnee Senawong Utane Rungpanich Pinklow Umrod Alan Maleesatharn Nantaka Kongstan watcharee Lermankul Kulkanya Chokephaibulkit Department of Pediatrics Faculty of Medicine Siriraj HospitalMahidol UniversityBangkokThailand Department of Immunology Faculty of Medicine Siriraj HospitalMahidol UniversityBangkokThailand

Objective:To evaluate the performance of interferon gamma release assays and tuberculin skin test in Bacillus Calmette-Guerin vaccinated young ***:A cross-sectional study was conducted in healthy children younger than 5 years who were recently diagnosed with tuberculosis or had recent exposure to active ***-TB Gold,*** and tuberculin skin test were performed in each ***:Of the 60 children,median age 3.3 years,17 had tuberculosis and 43 had recent tuberculosis ***,15(25.0%)children had tuberculin skin test reaction≥10 mm;8(13.3%)were positive by QuantiFERON-TB Gold In-Tube test,and 12(20.0%)by ***(31.7%)children had at least one positive *** was a moderate agreement between interferon gamma release assays and tuberculin skin ***:The positive rates of interferon gamma release assays and tuberculin skin test were low in young children who were infected with tuberculosis,supporting the management strategy of not testing children younger than 5 years.

关键词： Tuberculin skin test QuantiFERON-TB Gold In-Tube test T-SPOT.TB Young children

Anti-Inflammatory Activity of Geldanamycin and Its Derivatives in LPS-Induced RAW 264.7 Cells

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Advances in Microbiology 2019年第4期9卷 398-421页

作者： Thongchai Taechowisan Winyou Puckdee watcharee Waratchareeyakul Waya S. Phutdhawong Department of Microbiology Faculty of Science Silpakorn University Nakhon Pathom Thailand Department of Biology Faculty of Science and Technology Rambhai Barni Rajabhat University Chanthaburi Thailand Department of Chemistry Faculty of Science and Technology Rambhai Barni Rajabhat University Chanthaburi Thailand Department of Chemistry Faculty of Science Silpakorn University Nakhon Pathom Thailand

Geldanamycin (1) had been isolated as a major compound from Streptomyces zerumbet W14;an endophyte of Zingiber zerumbet (L.) Smith. Two new geldanamycin derivatives;17-(tryptamine)-17-demethoxygeldanamycin (2) and 17-(5’-methoxytryptamine)-17-demethoxygeldanamycin (3) were synthe- sized and their anti-inflammatory activity was evaluated in LPS-induced macrophage RAW 264.7 cells by investigating their effects on the inhibition of production of NO, PGE2, TNF-α, IL-1β, IL-6 and IL-10. The data obtained were consistent with the modulation of TNF-α, IL-1β, IL-6, IL-10 production by these derivatives at concentration of 1 to 5 μg/ml. A similar effect was also observed when LPS-induced NO release and PGE2 production were tested. The inhibitory effects were shown in concentration-dependent manners. From the obtained results, it was concluded that two new gelda- namycin derivatives possess anti-inflammatory activity on LPS-induced RAW 264.7 cells. They could be useful for the management of inflammatory diseases.

关键词： Anti-Inflammatory Activity Geldanamycin Derivatives Streptomyces zerumbet RAW 264.7 Cells

Application of Capture and Recapture Method for Estimating the Population Size of Men Who Have Sex with Men in Chiang Mai, Thailand

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World Journal of AIDS 2016年第4期6卷 197-204页

作者： Phrutthinun Surit Ning Zheng He Yi Xiaojing Yu watcharee Srithong Saisiri Mirasena Wutthichai Jariya Faculty of Medical Science Naresuan University Phitsanulok Thailand Center of Excellence in Medical Biotechnology (CEMB) Phitsanulok Thailand Shanghai Municipal Center for Disease Control & Prevention Shanghai China Faculty of Public Health Naresuan University Phitsanulok Thailand

Background: Men who have sex with men (MSM) are highly vulnerable to HIV infection, but this population can be particularly difficult to reach in Thailand. This study aimed to estimate the number of MSM in Chiang Mai Thailand, in order to plan HIV control and prevention. Methods: The total of 348 potential MSM were identified at eight contact locations, including two public parks, two bars, two massage parlors and two pubs in Chiang Mai. Trained 16 research enumerators and 16 enumerators were trained to extend a recruitment of brooches to MSM. The MSMs were captured one week apart. A record was kept of when, where and by whom the invitation was extended and received, and of refusals. The total estimate of MSM was derived from capture-recapture calculation. Results: The total MSM in Chiang Mai Thailand estimated by capture and recapture method was 733 (95%CI = 480 - 948) or the estimate was 7.3% (95%CI = 7.1% - 14.1%). Conclusions: Capture and recapture method can be used to enumerate and provide accurate and reliable estimates of the MSM population in any area, provided that certain conditions are controlled. MSM who are engaged in personal community represent a sizeable population who urgently need to be targeted by HIV and STDs prevention strategies.

关键词： MSM HIV Capture and Recapture Thailand

Risk Factors Affecting Condom Use among Royal Thai Army Conscripts in Thailand

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World Journal of AIDS 2017年第2期7卷 92-105页

作者： Phrutthinun Surit Wutthichai Jariya Ning Zheng He Yi Xiaojing Yu watcharee Srithong Saisiri Mirasena Faculty of Medical Science Naresuan University Phitsanulok Thailand Center of Excellence in Medical Biotechnology (CEMB) Naresuan University Phitsanulok Thailand Faculty of Public Health Naresuan University Phitsanulok Thailand Shanghai Municipal Center for Disease Control & Prevention Shanghai China

The study was conducted to explore factors associated with condom use of young Thai men conscripts. Methods: A cross-sectional study was conducted. Participants were 159 conscripts in second year who served in the Royal Thai Army by lottery method. Demographic data, sexual history including condom use, HIV and STDs and knowledge were collected using a self-administered questionnaire. Logistic regression was used to analyze the data. Results: Condom use at the last sex was 41.18% of the conscripts aged 20 - 27 years and secondary school education completed of 74.2%. 25.78% had experience with drugs used but injection was rare (6.9%). Age at the first sex was reported lowest at 11 years, 9.4% reported first sex with men and 21.38% were married. The participants have sexual history (last six months) with female only of 83.2%, male only of 4.1% and both of 14.6%. Among these only 12.6% reported every time of condom use and 19.5% never used condom. Older age, higher education, drinking alcohol, drugs use and higher knowledge were associated with condom use with statistical significance. Conclusions: Condom use among male Thai conscripts was low. Education and a condom use program are urgently needed to prevent future spread of HIV and STDs.

关键词： Conscript Condom AIDs Sexually Transmitted Diseases Thailand

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考虑加班因素的订单接受问题优化模型与算法

工业工程 2015年第2期18卷 1-8页

作者：陈淑玲李铁克王柏琳北京科技大学东凌经济管理学院北京100083 北京科技大学教育部钢铁生产制造执行系统技术工程研究中心北京100083

加班是制造商在面对客户需求增多时经常会采取的一种临时扩大产能的方法。本文将加班因素引入订单接受问题,考虑到加班容易导致生产线的工位间同时存在产能不足与产能过剩的情况,建立了以总利润最大化和生产线产能均衡为优化目标的数学... 详细信息

加班是制造商在面对客户需求增多时经常会采取的一种临时扩大产能的方法。本文将加班因素引入订单接受问题,考虑到加班容易导致生产线的工位间同时存在产能不足与产能过剩的情况,建立了以总利润最大化和生产线产能均衡为优化目标的数学模型。根据模型特征,本文采用两种分布式估计算法,即基于节点矩阵抽样算法和基于节点的偶合算法来实现问题求解。实验结果表明,基于节点的偶合算法具有良好的求解效果,并且产能均衡这一优化目标的设定可以在保证生产线产能平衡的同时有效地提高产品利润。

关键词：订单接受问题加班产能均衡分布式估计算法基于节点的偶合算法