检索结果-南通市图书馆

Chromosomal localization of a novel retinoic acid induced gene RA28 and protein distribution of its encoded protein

第36届中国控制会议

作者： Yang Yang xiuqin wang College of Engineering Bohai University College of Information Science and Technology Bohai University

ISBN: (纸本)9781538629185

Model inaccuracies or parameter uncertainties are unavoidable in the practical control systems, while the uncertain properties could be modeled and estimated by the grey system. Among many grey models, fractional grey model is recently proposed and popularly used in many model analysis and prediction problems. In this paper, the structure uncertainties and external disturbances are considered using the fractional grey model for control problem. When the model of the fractional grey controlled object and its parameters range is known, based on the model properties, it can be also approximated by a high order one, then the satisfactory adaptive ability and robustness can be obtained by the H-infinite optimal control. In the numerical simulation part, the corresponding results demonstrate the effectiveness of the robust fractional grey control algorithm, and it can be used for other grey control and related problems.

关键词： H-infinite control grey system fractional grey model uncertain information

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2000年第20期45卷 1857-1862页

作者： LIU Zhihua LUO Aiping wang xiuqin WU Min National Laboratory of Molecular Oncology Department of Cell BiologyCancer InstituteChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100021China

Gene RA28 is a retinoic acid induced novel gene isolated in our laboratory previously. All-trans retinoic acid (ATRA) was used to induce lung adenocarcinoma cell line GLC-82, and RA28 was obtained by subtractive hybridization. Green fluorescent protein (GFP) has emerged as a unique tool for examining introcellular phenomena in living cells. GFP possesses an intrinsic fluorescence at 488 nm that does not require other co-factors. In this report, an eukaryotic expression plasmid pEGFP-C1-RA28 was constructed and transfected with parental cell line GLC-82 to analyze protein expression and its distribution in living cells. Moreover, radiation hybrid (RH) technique was used to localize RA28 to the chromosome. The results show that gene RA28 is mapped to the chromosome 19q13.1 region, its encoded protein is distributed on cell membrane. All the results further demonstrate that GFP and RH techniques are accurate, fast, repetitive, and will be powerful methods for investigating the gene and protein

关键词： green fluorescent protein (GFP) radiation hybrid (RH) gene localization protein localization RA28.

Identification of differentially expressed genes in esophageal cancer through SSH in combination with high throughput reverse Northern screening

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2001年第14期46卷 1195-1198页

作者： ZHOU Jin LIU Zhihua wang xiuqin DING Fang ZHOU Chuannong WU Min National Laboratory of Molecular Oncology Department of Cell BiologyCancer InstitutePeking Union Medical College and Chinese Academy of Medical SciencesBeijing 100021China

To understand the molecular mechanisms of carcinogenesis of esophagus and to isolate genes with different expression levels in esophageal cancer, suppression sub-tractive hybridization (SSH) was combined with PCR-based cDNA synthesis and reverse Northern on the cancer tissues and matched almost normal mucosa using 5 microgram of total RNA as starting marterial. Eight genes were found expressed differentially in esophageal cancer, in which 5 were known genes and 3 were novel ones; and 6 were down-regulated in cancer tissues, while 2 were up-regulated; 6 were of mid-high abundance and 2 were of low abundance in esophagus. The results revealed that alteration in expression level of multiple genes underlied the initiation and development of esophageal cancer. The differentially expressed genes identified in this study such as liporcotin I, cystatin A, cystatin B, cytokeratin 13 may play roles in dedifferentiation, transformation and malignant proliferation of esophageal cancer. The combination of SSH with

关键词： esophageal cancer suppression subtractive hybridization (SSH) differential expression lipocortin cystatin.

Optical Properties and Source Analysis of Aerosols over a Desert Area in Dunhuang,Northwest China

在线全文

学校读者我要写书评

暂无评论

Advances in Atmospheric Sciences 2017年第8期34卷 1017-1026页

作者： Yongjing MA Jinyuan XIN Yining MA Lingbin KONG Kequan ZHANG Wenyu ZHANG Yuesi wang xiuqin wang Yongfeng ZHU College of Atmospheric Sciences Key Laboratory of Arid Climatic Change and Reducing Disaster of Gansu ProvinceLanzhou UniversityLanzhou 730000China State Key Laboratory of Atmospheric Boundary Layer Physics and Atmospheric Chemistry Institute of Atmospheric PhysicsChinese Academy of SciencesBeijing 100029China College of Atmospheric Sciences Chengdu University of Information TechnologyPlateau Atmosphere and Environment Key Laboratory of Sichuan ProvinceChengdu 610225China Dunhuang Meteorological Bureau Dunhuang 736200China

Aerosol observational data for 2012 obtained from Dunhuang Station of CARE-China（Campaign on Atmospheric Aerosol Research Network of China） were analyzed to achieve in-depth knowledge of aerosol optical properties over Dunhuang region. The results showed that the annual average aerosol optical depth（AOD） at 500 nm was 0.32 ± 0.06, and the ？ngstr？m exponent（α） was 0.73 ± 0.27. Aerosol optical properties revealed significant seasonal characteristics. Frequent sandstorms in MAM（March–April–May） resulted in the seasonal maximum AOD, 0.41 ± 0.04, and a relatively smaller αvalue, 0.44 ± 0.04. The tourism seasons, JJA（June–July–August） and SON（September–October–November） coincide with serious emissions of small anthropogenic aerosols. While in DJF（December–January–February）, the composition of the atmosphere was a mixture of dust particles and polluted aerosols released by domestic heating; the average AOD and αwere 0.29 ± 0.02 and 0.66 ± 0.17, respectively. Different air masses exhibited different degrees of influence on the aerosol concentration over Dunhuang in different seasons. During MAM, ranges of AOD（0.11–1.18） and α（0.06–0.82） were the largest under the dust influence of northwest-short-distance air mass in the four trajectories. Urban aerosols transported by northwest-short-distance air mass accounted for a very large proportion in JJA and the mixed aerosols observed in SON were mainly conveyed by air masses from the west. In DJF, the similar ranges of AOD and α under the three air mass demonstrated the analogous diffusion effects on regional pollutants over Dunhuang.

关键词： Dunhuang AOD ?ngstr?m exponent dust aerosol anthropogenic aerosols

Antioxidant effects of the aqueous extract of turmeric against hydrogen peroxide-induced oxidative stress in spotted seabass(Lateolabrax maculatus)

在线全文

学校读者我要写书评

暂无评论

Aquaculture and Fisheries 2024年第1期9卷 71-77页

作者： Zhe wang xiuqin wang Xueshan Li Kangle Lu Ling wang Xuekun Ma Kai Song Chunxiao Zhang Xiamen Key Laboratory for Feed Quality Testing and Safety Evaluation Fisheries College of Jimei UniversityXiamen361021China Guangdong Yuehai Feed Group Co. Ltd.GuangdongZhanjiang524000China

Intensive aquaculture-induced oxidative stress is detrimental to fish health and *** plants show promise as natural health boosters and antioxidants in the aquaculture ***,this work investigated the effects of turmeric aqueous extract(TAE)on the growth performance,antioxidant status,and hydrogen peroxide(H2O2)-induced oxidative stress in spotted seabass(Lateolabrax maculatus).Fish were fed diets supplemented with 0(Con),2(TAE2),or 4(TAE4)g/kg TAE for eight weeks,then were injected with *** results showed that dietary supplementation of TAE did not affect fish growth,feed utilization,or body *** treatment increased liver antioxidant enzyme activities and decreased liver malondialdehyde content and serum levels of glutamate oxalate transaminase,glutamate pyruvate transaminase,and lactate ***,the increases in mortality,liver malondialdehyde content,and serum biomarkers of liver injury in the H2O2-treated fish were inhibited as a consequence of the TAE *** addition,TAE treatment activated the Nrf2/Keap1 pathway in the liver,supported by the up-regulated expression of nrf2,ho-1,and gclc,and down-regulated keap1 ***,dietary incorporation of TAE protected against H2O2-induced oxidative stress in spotted seabass probably by enhancing antioxidant capacity through the Nrf2/Keap1 pathway.

关键词： Turmeric Medicinal plant Hydrogen peroxide Oxidative stress Lateolabrax maculatus

Zhang Huoding： Personification Helps Peking Opera Go Global

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

China & The World Cultural Exchange 2015年第11期81卷 12-19页

作者： wang xiuqin

On Sep. 2 and 3, 2015, two China-related events happened almost simultaneously in the east and west hemispheres across the Pacific Ocean： one is China＇s V-day Parade in Tiananmen Square; the other is the Peking Opera show by famous Chinese artist Zhang Huoding in Lincoln Center for the Performing Arts, in New York. Zhang performed the classics of The Legend of White Snake and The .Jewelry, Purse. With her perfect skills of singing, dialoguing, acting and acrobatic combat, Zhang demonstrated the essence of Peking Opera to American audiences.

关键词：京剧人格化艺术中心珠宝首饰中国情人节太平洋天安门

Long Distance Separates No Like-minded People--On 2016 China-Latin America Cultural Exchange Year

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

China & The World Cultural Exchange 2016年第12期82卷 8-12页

作者： wang xiuqin

This year, nearly an audience of 10 million people participated in the 2016 China-Latin America Cultural Exchange Year hosted by the Ministry of Culture and the China Arts and Entertainment Group. As the largest cultural event between China and Latin America since the establishment of the PRC, the Cultural Exchange Year presents the culture of Latin America and China to the world.

关键词：英语阅读理解文化交流

Differential expression analysis of matrix metallo-proteinase 20(MMP20) in human esophageal cancer

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2001年第12期46卷 1009-1012,1057页

作者： YANG Cuihong LIU Zhihua wang Qun LUO Aiping SUN Yuntian ZHANG Xun wang xiuqin ZHOU Chuannong WU Min National Key Laboratory of Molecular Oncology Cancer InstituteChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100021China Department of Pathology Cancer InstituteChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100021China

The aim of this study was to investigate the different expressions of MMP20 in the process of esophageal cancer. RT-PCR, immunohistochemistry and Western blot were employed to detect the mRNA expression and protein expression of MMP20. Compared with the corresponding normal esophagus tissues, results showed the common trend of the dramatical overexpression of MMP20 in the esophageal cancer tissues. The analysis of MMP20 expression in different clinical early stages of esophageal cancer by RT-PCR indicated that MMP20 was much more overexpressed in the esophageal cancer with invasion than in the early stages. This suggested that MMP20 might play an important role in the progression of esophageal cancer. It probably plays a critical role in tumor invasion.

关键词： esophageal cancer MMP20 RT-PCR Western blot.

Suppression of charge recombination by application of CueZnSnS4-graphene counter electrode to thin dye-sensitized solar cells

在线全文

学校读者我要写书评

暂无评论

Science Bulletin 2016年第15期61卷 1221-1230页

作者： Yan Li Huafei Guo xiuqin wang Ningyi Yuan Jianning Ding Collaborative Innovation Center forPhotovoltaic Science and Engineering Jiangsu Province Cultivation Base for State KeyLaboratory of Photovoltaic Science and Technology ChangzhouUniversity Changzhou 213164 China Micro/Nano Science and Technology Center Jiangsu UniversityZhenjiang 212013 China

This paper proposes a new mechanism to explain the performance of thin dye-sensitized solar cells （DSSC）. Near-stoichiometric flower-like Cu2ZnSnS4 （CZTS） microspheres with a high specific surface area was fabri- cated for use as the photocathode in a DSSC. To improve the extraction and transfer of electrons, graphene was added to the CZTS. A DSSC with a 10-gin TiO2 pho- toanode layer exhibited a slightly degraded efficiency with a CZTS-graphene photocathode, relative to a Pt counter electrode （CE）. Nevertheless, when the thickness of the TiO2 photoanode was reduced to 2 lam, the efficiency of a DSSC with a CZTS-graphene photocathode was greater than that of a Pt-DSSC. It is speculated that, unlike the Pt CE, a CZTS-graphene photocathode not only collects electrons from an external circuit and catalyzes the reduction of the triiodide ions in the electrolyte, but also utilizes unabsorbed photons to produce photo-excited electrons and suppresses charge recombination, thus enhancing the performance of the cell. The use of narrowband gap p-type semiconductors as photocathodes offers a new means of fabricating thin dye-sensitized solar cells and effectively improving the cell performance.

关键词： Dye-sensitized solar cell Cu2ZnSnS4-graphene Charge recombination