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Development of a recombinant pB602L-based indirect ELISA assay for detecting antibodies against African swine fever virus in pigs

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Journal of Integrative Agriculture 2022年第3期21卷 819-825页

作者： wang Peng-fei wang Ming shi Zhi-bin SUN Zhen-zhao WEI Li-li LIU Zai-si wang shi-da HE Xi-jun wang Jing-fei State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069P.R.China

African swine fever(ASF),caused by the African swine fever virus(ASFV),is a devastating disease of domestic and wild *** is no effective vaccine,and the control of the disease relies mainly on surveillance and early detection of infected ***,serological assays,such as ELISA,have been developed mainly based on recombinant structural viral proteins of ASFV,including p72,p54,and ***,the antibodies against these proteins do not provide efficient protection against ASFV infection in ***,new serological assays that can be applied for clinical diagnosis and evaluating serological immune response in vaccinated pigs are still *** this study,we expressed and purified a recombinant p B602 L *** purified p B602 L protein was then used as an antigen to develop an indirect ELISA *** assay has no cross-reaction with the anti-sera against the 15 most common pig pathogens in China,such as classical swine fever virus,pseudorabies virus,and porcine *** assay and a commercial ELISA kit were then used to detect 60 field pig serum samples,including an unknown number of antiASFV *** coincidence of the two assays was 95%.Furthermore,the p B602 L-based ELISA was employed to test the antibody responses to the seven-gene-deleted ASFV strain HLJ/18-7 GD in *** results showed that the antibody levels in all vaccinated pigs,starting from the 10 th day post-inoculation,have increased continuously during the observation period of 45 *** results indicate that this p B602 L-based indirect ELISA assay can be employed potentially in the field of ASFV diagnosis.

关键词： African swine fever virus pB602L recombinant protein indirect ELISA assay pig

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Composite Sophora Colon-Soluble Capsule Ameliorates DSS-Induced Ulcerative Colitis in Mice via Gut Microbiota-Derived Butyric Acid and NCR+ ILC3

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Chinese Journal of Integrative Medicine 2023年第5期29卷 424-433页

作者： CHEN Ming-jun FENG Yang GAO Lu LIN Ming-xiong wang shi-da TONG Zhan-qi Medical School of Chinese People's Liberation Army Beijing(100853)China Department of Traditional Chinese Medicine The Second Medical Center&National Clinical Research Center of Geriatric DiseasesChinese People's Liberation Army General HospitalBeijing(100853)China Institute of Military Cognition and Brain Sciences Academy of Military Medical SciencesAcademy of Military SciencesBeijing(100850)China

Objective: To investigate the effects of composite Sophora colon-soluble Capsule(CSCC) on gut microbiota-mediated short-chain fatty acids(SCFAs) production and downstream group 3 innate lymphoid cells(ILC3s) of dextran sulfate sodium(DSS)-induced ulcerative colitis(UC) mice model. Methods: The main components of CSCC were analyzed by hybrid ultra-high-performance liquid chromatography ion mobility spectromety quadrupole time-of-flight mass spectrometry(UHPLC-IM-QTOF/MS). Twenty-four male BALB/c mice were randomly divided into 4 groups(n=6) by using a computer algorithm-generated random digital, including control, DSS model, mesalazine, and CSCC groups. A DSS-induced colitis mice model was established to determine the effects of CSCC by recording colonic weight, colonic length, index of colonic weight, and histological colonic score. The variations in ILC3s were assessed by immunofluorescence and flow cytometry. The results of gut microbiota and SCFAs were acquired by 16s r DNA and gas chromatography-mass spectrometry(GC-MS) analysis. The expression levels of NCR^(+)ILC3^(-), CCR6^(+)Nkp46-(Lti) ILC3^(-), and ILCreg-specific markers were detected by enzyme-linked immunosorbent assay, and real-time quantitative polymerase chain reaction and Western blot, respectively. Results: The main components of CSCC were matrine, ammothamnine, Sophora flavescens neoalcohol J, and Sophora oxytol U. After 7 days of treatment, CSCC significantly alleviated colitis by promoting the reproduction of intestinal probiotics manifested as upregulation of the abundance of Bacteroidetes species and specifically the Bacteroidales_S24-7 genus(P<0.05). Among the SCFAs, the content of butyric acid increased the most after CSCC treatment. Meanwhile, compared with the model group, Lti ILC3s and its biomarkers were significantly downregulated and NCR^(+) ILC3s were significantly elevated in the CSCC group(P<0.01). Further experiments revealed that ILC3s were differentiated from Lti ILC3s to NCR^(+)

关键词： composite Sophora colon-soluble capsule ulcerative colitis group 3 innate lymphoid cells gut microbiota butyric acid

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青岛特钢开炉经验及生产实践

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中国冶金 2018年第11期28卷 40-44页

作者：王世达青岛特殊钢铁有限公司炼铁厂

青岛特殊钢铁有限公司(以下简称青岛特钢)始建于2013年,是青钢集团为实现产业升级和可持续发展,在“十二五”期间通过实施搬迁新成立的新型环保钢铁企业。青岛特钢环保搬迁项目一期工程配套建设2×1800m3高炉,分别于2015年11月、2016年1... 详细信息

青岛特殊钢铁有限公司(以下简称青岛特钢)始建于2013年,是青钢集团为实现产业升级和可持续发展,在“十二五”期间通过实施搬迁新成立的新型环保钢铁企业。青岛特钢环保搬迁项目一期工程配套建设2×1800m3高炉,分别于2015年11月、2016年10月相继开炉投产,对两次开炉初期的一些问题和经验及后续的生产实践进行了总结,分享了开炉和生产实践中的成功经验,也对高炉炼铁合理操作炉型的维护、低成本经济炼铁等方面进行了深入论述。投产至今,两座高炉铁水成本、技术经济指标均达到同行业先进水平。

关键词：大型高炉开炉操作炉型经济炼铁

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Flexible molecules dedicate to release strain of inverted inorganic perovskite solar cell

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Journal of Energy Chemistry 2025年第1期100卷 87-93页

作者： Hongrui Sun Sanlong wang Pengyang wang Yali Liu Shanshan Qi Biao shi Ying Zhao Xiaodan Zhang Institute of Photoelectronic Thin Film Devices and Technology Renewable Energy Conversion and Storage Center State Key Laboratory of Photovoltaic Materials and CellsNankai University Key Laboratory of Photoelectronic Thin Film Devices and Technology of Tianjin Haihe Laboratory of Sustainable Chemical Transformations Engineering Research Center of Thin Film Photoelectronic Technology of Ministry of Education Collaborative Innovation Center of Chemical Science and Engineering (Tianjin)

The tensile strain in inorganic perovskite films induced by thermal annealing is one of the primary factors contributing to the inefficiency and instability of inorganic perovskite solar cells （IPSCs）,which reduces the defect formation ***,a flexible molecule 5-maleimidovaleric acid （5-MVA） was introduced as a strain buffer to release the residual strain of *** anhydride and carboxyl groups in 5-MVA interact strongly with the uncoordinated Pb2+through Lewis acid-base reaction,thus tightly“pull”the perovskite *** in-between soft carbon chain increased the structural flexibility of CsPbI2.85Br0.15perovskite materials,which effectively relieved the intrinsic internal strain of CsPbI2.85Br0.15,resisted the corrosion of external strain,and also reduced the formation of defects such as VIand *** addition,the introduction of 5-MVA improved crystal quality,passivated residual defects,and narrowed energy level ***,power conversion efficiency （PCE） of NiOxbased inverted IPSCs increased from 19.25% to 20.82% with the open-circuit voltage enhanced from 1.164 V to 1.230 *** release of strain also improved the stability of CsPbI2.85Br0.15perovskite films and devices.

关键词： Inverted inorganic perovskite solar cells Flexible molecules Strain release Crystallization Energy barrier High PCE

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浅谈防爆电气产品现场检测工作的重要性

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电气开关 2016年第6期54卷 104-106页

作者：王世达辽河油田安全环保处特检中心辽宁盘锦124010

针对防爆电气设备现场检测过程中发现的问题,进行了探讨并提出了相应措施进行了专业性的探讨。

关键词：防爆电气设备检测依据安全隐患风险分析措施

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The pivotal role of microglia in injury and the prognosis of subarachnoid hemorrhage

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Neural Regeneration Research 2025年第7期20卷 1829-1848页

作者： Wenjing Ning shi Lv Qian wang Yuzhen Xu Department of Rehabilitation The Second Affiliated Hospital of Shandong First Medical UniversityTaianShandong ProvinceChina Department of Central Laboratory The Affiliated Taian City Central Hospital of Qingdao UniversityTaianShandong ProvinceChina

Subarachnoid hemorrhage leads to a series of pathological changes,including vascular spasm,cellular apoptosis,blood–brain barrier damage,cerebral edema,and white matter ***,which are the key immune cells in the central nervous system,maintain homeostasis in the neural environment,support neurons,mediate apoptosis,participate in immune regulation,and have neuroprotective *** evidence has shown that microglia play a pivotal role in the pathogenesis of subarachnoid hemorrhage and affect the process of injury and the prognosis of subarachnoid ***,microglia play certain neuroprotective roles in the recovery phase of subarachnoid *** approaches aimed at modulating microglia function are believed to attenuate subarachnoid hemorrhage *** provides new targets and ideas for the treatment of subarachnoid ***,an in-depth and comprehensive summary of the role of microglia after subarachnoid hemorrhage is still *** review describes the activation of microglia after subarachnoid hemorrhage and their roles in the pathological processes of vasospasm,neuroinflammation,neuronal apoptosis,blood–brain barrier disruption,cerebral edema,and cerebral white matter *** also discusses the neuroprotective roles of microglia during recovery from subarachnoid hemorrhage and therapeutic advances aimed at modulating microglial function after subarachnoid ***,microglia in subarachnoid hemorrhage are targeted with TLR inhibitors,nuclear factor-κB and STAT3 pathway inhibitors,glycine/tyrosine kinases,NLRP3 signaling pathway inhibitors,Gasdermin D inhibitors,vincristine receptorαreceptor agonists,ferroptosis inhibitors,genetic modification techniques,stem cell therapies,and traditional Chinese ***,most of these are still being evaluated at the laboratory *** clinical studies and data on subarachnoid hemorrhage are required to improve the treatment of subarachnoid hemor

关键词： apoptosis blood–brain barrier brain edema microglia neuroinflammation neuron neuroprotection subarachnoid hemorrhage vasoconstriction white matter injury

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复合B族维生素对大鼠应激性认知功能障碍发生中的保护性作用

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营养学报 2018年第2期40卷 152-157页

作者：王世达张亮赵云马婧王雪谢方钱令嘉军事医学研究院军事认知与脑科学研究所北京100850

目的研究复合B族维生素(VBco)对大鼠应激性认知功能障碍发生过程中的保护性作用。方法选用成年雄性SD大鼠,分为5组,每组30只。实验组采用慢性不可预见性应激(CUMS)模型干预;干预组[CUMS+VBco+FA(叶酸)]:在CUMS过程中给予VBco灌胃[VB6(24... 详细信息

目的研究复合B族维生素(VBco)对大鼠应激性认知功能障碍发生过程中的保护性作用。方法选用成年雄性SD大鼠,分为5组,每组30只。实验组采用慢性不可预见性应激(CUMS)模型干预;干预组[CUMS+VBco+FA(叶酸)]:在CUMS过程中给予VBco灌胃[VB6(24 mg/kg bw·d),VB12(20μg/kg bw·d),叶酸(10mg/kg bw·d)];VBco对照组(VBco)仅给予VBco灌胃,添加量同VBco干预组;高蛋氨酸饮食组(Met)使用含1%蛋氨酸特殊饲料饲喂;空白对照组(Ctrl)不做特殊处理。动物认知功能采用旷场实验、Morris水迷宫实验、电跳台实验及探物实验检测。血浆同型半胱氨酸(Hcy)采用高效液相色谱(HPLC)分析方法检测。各组干预时程均为8w,分别于第0、2、4、6、8w末进行行为学实验,并检测血浆Hcy水平。结果持续的CUMS导致大鼠各项认知功能显著性降低,血浆Hcy水平显著性升高;应激过程中给予VBco可有效缓解大鼠认知功能下降,并翻转CUMS诱导的血浆Hcy水平上升趋势;高蛋氨酸饮食显著升高血浆Hcy水平,并降低各项认知功能;单纯给予VBco对血浆Hcy水平及认知功能无明显影响。结论慢性应激可诱导大鼠认知功能障碍,VBco能够有效缓解应激大鼠认知障碍的发生。VBco的作用可能与其阻抑应激大鼠血浆Hcy水平有关。

关键词：复合维生素慢性应激认知功能障碍同型半胱氨酸大鼠

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生脉注射液联用酚妥拉明治疗肺心病心力衰竭疗效观察

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中国热带医学 2012年第1期12卷 120-121页

作者：王大士儋州市三都镇卫生院海南儋州571744

目的观察生脉注射液联用酚妥拉明治疗慢性肺源性心脏病难治性心力衰竭临床疗效。方法选择慢性肺源性心脏病心力衰竭患者58例,随机分为两组,治疗组28例,对照组30例。两组均给予休息,低盐低脂饮食,间断低流量吸氧,抗感染、解痉平喘、强心... 详细信息

目的观察生脉注射液联用酚妥拉明治疗慢性肺源性心脏病难治性心力衰竭临床疗效。方法选择慢性肺源性心脏病心力衰竭患者58例,随机分为两组,治疗组28例,对照组30例。两组均给予休息,低盐低脂饮食,间断低流量吸氧,抗感染、解痉平喘、强心、利尿、扩血管等常规抗心力衰竭治疗,治疗组在上述治疗的基础上加用生脉注射液(60ml/d)及酚妥拉明(20mg/d),7d为一个疗程,共治疗2个疗程。结果根据患者咳、喘症状、肺部啰音、心功能改善等指标分级,治疗组显效17例,有效9例,无效2例。总有效率为92.85%,对照组显效11例,有效12例,无效8例,总有效率为73.33%,两组总有效率比较差异有统计学意义(χ2=5.71,P<0.05)。结论生脉注射液联用酚妥拉明对肺心病难治性心力衰竭有效,且无明显副作用,值得推广应用。

关键词：肺心病难治性心力衰竭生脉注射液酚妥拉明

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早年应激致雌性大鼠认知功能障碍中脑脊液氨基酸变化及意义

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营养学报 2022年第2期44卷 164-172页

作者：王笑天谢方王雪王世达赵云钱令嘉军事科学院军事医学研究院军事认知与脑科学研究所北京100850

目的研究母婴分离应激雌性大鼠成年后脑脊液(cerebro-spinal fluid,CSF)氨基酸的变化,为探索早年应激致认知障碍的发生机制提供新的科学依据。方法采用母婴分离(maternal separation,MS)应激的方法建立早年应激大鼠模型,随机分对照组(... 详细信息

目的研究母婴分离应激雌性大鼠成年后脑脊液(cerebro-spinal fluid,CSF)氨基酸的变化,为探索早年应激致认知障碍的发生机制提供新的科学依据。方法采用母婴分离(maternal separation,MS)应激的方法建立早年应激大鼠模型,随机分对照组(CON)和应激组(MS)。在实验大鼠成年后采用旷场、物体识别及水迷宫实验评价认知功能,尼氏染色观察脑海马和前额叶皮质区神经细胞数量及排列,全自动蛋白表达分析系统(WESTM)检测突触后致密物95(PSD-95)蛋白表达水平,超高效液相色谱-串联质谱(UPLC-MS/MS)靶向定量分析大鼠脑脊液氨基酸含量。结果与CON组大鼠相比,MS组大鼠旷场评分、物体识别认知指数、水迷宫穿台次数显著降低(P0)。结论母婴分离应激致大鼠成年后认知功能障碍,脑相关区域损伤和脑脊液氨基酸代谢异常,以及苏氨酸水平降低影响神经细胞突触可塑性可能是应激性认知功能障碍的病理基础。[营养学报,2022,44(2):164-172]

关键词：早年应激雌性大鼠脑脊液氨基酸代谢突触可塑性认知障碍

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实验动态可视微循环与活血化瘀研究

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中国中西医结合杂志 2017年第3期37卷 371-376页

作者：寇娜薛梅王铭铭史大卓北京中医药大学研究生院北京100029 中国中医科学院西苑医院心血管中心北京100091

动态可视化微循环技术是采用可视化技术,结合荧光标记、高速录像、电镜等方法观察微循环的变化,将静息图片转换为动态可视图形的技术。近十年来,国内研究者采用此技术,针对心脏、软脑膜、肠系膜微循环展开研究,动态观察活血化瘀单体、... 详细信息

动态可视化微循环技术是采用可视化技术,结合荧光标记、高速录像、电镜等方法观察微循环的变化,将静息图片转换为动态可视图形的技术。近十年来,国内研究者采用此技术,针对心脏、软脑膜、肠系膜微循环展开研究,动态观察活血化瘀单体、单味中药与中药复方对微动脉/微静脉管径、红细胞流速、白细胞黏附、血小板聚集、微血管通透性、过氧化物产生及血浆白蛋白漏出等的影响,阐释了活血化瘀中药改善微循环的作用机理。进一步优化微循环检测技术,发挥动态可视化微循环技术直观优势,系统评价中药多部位、多靶点的作用机制及与中西药间的相互作用机制,是未来微循环研究的重要方向。

关键词：微循环动态可视化技术活血化瘀

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