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Waxy allelic diversity in common millet(Panicum miliaceum L.) in China

The Crop Journal 2018年第4期6卷 377-385页

作者： Ruiyun wang Haigang wang Xiaohuan Liu Xu Ji Ling Chen ping Lu Minxuan Liu Bin Teng Zhijun Qiao College of Agriculture Shanxi Agricultural University Institute of Crop Germplasm Resources of Shanxi Academy of Agricultural Sciences Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau Ministry of Agriculture Shanxi Key Laboratory of Genetic Resources and Genetic Improvement of Minor Crops Institute of Crop Science Chinese Academy of Agricultural Sciences Anhui Provincial Key Lab of Rice Genetics and Breeding Institute of Rice Research Anhui Academy of Agricultural Sciences

A set of 132 accessions of commonmillet, Panicum miliaceum L., from12 provinces of China were assessed for endosperm starch type （waxy or non-waxy） using I2-KI staining, amylose and amylopectin contents using the dual-wavelength colorimetric method, and genotype of Waxygenes conditioning amylose content by gene sequencing. Endosperm starch content varied from 57.69% to 74.70%, while the amylose and amylopectin contents of the starch ranged from 0 to 23.29% and from 41.99% to 70.24%, respectively. Sequencing two Wx genes, including Wx-L （intron 5-exon 7 and intron 8–9） andWx-S （exon 9–intron 10） revealed several polymorphisms （S0, S？15, LC, LF, LY）. Marker M5-R11 linking to the Wx-S gene may be used to discriminate waxy common millet accessions from non-waxy ones. Among the 132 accessions, 68 with the S？15 allele had waxy endosperm starch with the amylose content range 0–2.58% and 64 accessions with the S0 allele had non-waxy endosperm starch with amylose content range 3.94%–23.29%. Five genotypes including So15/LF （45% of the accessions）, S0/LF （25%）, S0/LY （12%）, S0/LC （11%）, and S？15/LY （7%） were identified. Six new SNPs were detected at the Wx-L locus. These results will facilitate common millet breeding, especially of cultivars free of amylose.

关键词： Common millet Waxy gene snp Starch

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Research progress in interface modification and thermal conduction behavior of diamond/metal composites

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International Journal of Minerals,Metallurgy and Materials 2022年第2期29卷 200-211页

作者： ping Zhu pingping wang Puzhen Shao Xiu Lin Ziyang Xiu Qiang Zhang Equo Kobayashi Gaohui Wu School of Materials Science and Engineering Harbin Institute of TechnologyHarbin 150001China Key Laboratory of Advanced Structure-Function Integrated Materials and Green Manufacturing Technology School of Material Science and EngineeringHarbin Institute of TechnologyHarbin 150001China Industrial Technology Research Institute of Heilongjiang Province Harbin 150001China Department of Materials Science and Engineering Tokyo Institute of TechnologyTokyo 152-8550Japan

Diamond/metal composites are widely used in aerospace and electronic packaging fields due to their outstanding high thermal conductivity and low ***,the difference in chemical properties leads to interface incompatibility between diamond and metal,which has a considerable impact on the performance of the *** improve the interface compatibility between diamond and metal,it is necessary to modify the interface of *** paper reviews the experimental research on interface modification and the application of computational simulation in diamond/metal *** computational simulation with experimental methods is a promising way to promote diamond/metal composite interface modification research.

关键词： diamond/metal interface modification thermal conductivity computational simulation

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Two new aristolane sesquiterpenes from Laurencia similis

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Chinese Chemical Letters 2007年第2期18卷 178-180页

作者： Nai Yun Ji Xiao Ming Li Lan ping Ding Bin Gui wang Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China Graduate School of the Chinese Academy of Sciences Beijing 100049 China

Two new aristolane sesquiterpenes, namely, aristolan-10-ol-9-one and aristolan-8-en-1-one, were isolated from the red alga Laurencia similis. Their structures were established on the basis of various NMR spectroscopic... 详细信息

关键词： Laurencia similis Aristolane Aristolan-10-ol-9-one Aristolan-8-en-1-one

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考虑质量控制和损失规避的供应链协调研究

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控制与决策 2018年第12期33卷 2295-2304页

作者：王道平赵超程延平北京科技大学东凌经济管理学院北京100083

研究质量控制和各成员均损失规避背景下的供应链协调问题,应用损失规避效应函数刻画制造商和零售商的损失规避行为,通过分析比较分散决策和集中决策两种模式下的最优策略,引入批发价格-质量成本分担契约构建供应链协调模型,研究表明,该... 详细信息

研究质量控制和各成员均损失规避背景下的供应链协调问题,应用损失规避效应函数刻画制造商和零售商的损失规避行为,通过分析比较分散决策和集中决策两种模式下的最优策略,引入批发价格-质量成本分担契约构建供应链协调模型,研究表明,该契约能够使质量控制与损失规避背景下的供应链实现协调.通过算例对损失规避程度、产品合格率和销售价格等参数进行灵敏度分析,揭示了损失规避和质量控制等因素与最优产品质量水平、最优订货量以及供应链效用之间的关系.

关键词：质量控制损失规避批发价格-质量成本分担契约供应链协调

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Temporal and Spatial Variations of Microbial Carbon Utilization in Water Bodies from the Dajiuhu Peatland, Central China

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Journal of Earth Science 2018年第4期29卷 969-976页

作者： Rui-cheng wang Hong-Mei wang Xing Xiang Yu Gao Qing-Wei Song Lin-Feng Gong State Key Laboratory of Biogeology and Environmental Geology China University of Geosciences Wuhan 430074 China State Key Laboratory Breeding Base of Marine Genetic Resources Key Laboratory of Marine Genetic Resources Third Institute of Oceanography SOA Key Laboratory of Marine Genetic Resources of Fujian Province Xiamen 361005 China

To investigate the microbial utilization of organic carbon in peatland ecosystem, water samples were collected from the Dajiuhu Peatland and nearby lakes, central China across the year of 2014. The acridine orange （AO） staining and Biolog Eco microplates were used to numerate microbial counts and determine the carbon utilization of microbial communities. Meanwhile, physicochemical characteristics were measured for subsequent analysis of the correlation between microbial carbon utilization and environmental factors. Results indicated that total microbial counts were between 106107 cells/L. Microbial diversities and carbon utilization rates showed a similar pattern, highest in September and lowest in November. Microbial communities in the peat pore waters preferred to utilize N-bearing carbon sources such as amines and amino acids compared with microbial communities in lakes. The network analysis of microbial utilization of 31 carbon substrates clearly distinguished microbial communities from peat pore waters and those from lakes. Redundancy analysis （RDA） showed the total organic nitrogen content （P=-0.03, F=2.5） and daily average temperature （P=0.034, F=2.4） significantly controlled microbial carbon utilization throughout the sampling period. Our report is the first one to address the temporal and spatial variations of carbon uti- lization of microbial communities which are closely related to the decomposition of organic matter in the Dajiuhu Peatland in context of climate warming.

关键词： carbon source microbial carbon utilization average temperature Dajiuhu Peatland.

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Hyodeoxycholic acid protects the neurovascular unit against oxygen-glucose deprivation and reoxygenation-induced injury in vitro

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Neural Regeneration Research 2019年第11期14卷 1941-1949页

作者： Chang-Xiang Li Xue-Qian wang Fa-Feng cheng Xin Yan Juan Luo Qing-Guo wang School of Traditional Chinese Medicine Beijing University of Chinese Medicine

Calculus bovis is commonly used for the treatment of stroke in traditional Chinese medicine. Hyodeoxycholic acid(HDCA) is a bioactive compound extracted from calculus bovis. When combined with cholic acid, baicalin and jas-minoidin, HDCA prevents hypoxia-reoxygenation-induced brain injury by suppressing endoplasmic reticulum stress-mediated apoptotic signaling. However, the effects of HDCA in ischemic stroke injury have not yet been studied. Neurovascular unit(NVU) dysfunction occurs in ischemic stroke. Therefore, in this study, we investigated the effects of HDCA on the NVU under ischemic conditions in vitro. We co-cultured primary brain microvascular endothelial cells, neurons and astrocytes using a transwell chamber co-culture system. The NVU was pre-treated with 10.16 or 2.54 μg/mL HDCA for 24 hours before exposure to oxygen-glucose deprivation for 1 hour. The cell counting kit-8 assay was used to detect cell activity. Flow cytometry and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling were used to assess apoptosis. Enzyme-linked immunosorbent assay was used to measure the expression levels of inflammatory cytokines, including interleukin-1β, interleukin-6 and tumor necrosis factor-α, and neurotrophic factors, including brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor. Oxidative stress-related factors, such as superoxide dismutase, nitric oxide, malondialdehyde and γ-glutamyltransferase, were measured using kits. Pretreatment with HDCA significantly decreased blood-brain barrier permeability and neuronal apoptosis, significantly increased transendothelial electrical resistance and γ-glutamyltransferase activity, attenuated oxidative stress damage and the release of inflammatory cytokines, and increased brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor expression. Our findings suggest that HDCA maintains NVU morphological integrity and function by modulating inflammation, oxidat

关键词： hyodeoxycholic acid oxygen glucose deprivation and reoxygenation blood-brain barrier permeability anti-oxidative anti-inflammatory anti-apoptotic brain-derived neurotrophic factor glial cell line-derived neurotrophic factor ischemic stroke in vitro neurovascular unit

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Tunable 2H–TaSe_2 room-temperature terahertz photodetector

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Chinese Physics B 2019年第4期28卷 18-22页

作者： Jin wang cheng Guo Wanlong Guo Lin wang wangzhou Shi Xiaoshuang Chen Department of Physics Shanghai Normal University State Key Laboratory for Infrared Physics Shanghai Institute of Technical Physics Chinese Academy of Sciences University of Chinese Academy of Sciences

Two-dimensional transition metal dichalcogenides(TMDs) provide fertile ground to study the interplay between dimensionality and electronic properties because they exhibit a variety of electronic phases, such as semiconducting, superconducting, charge density waves(CDW) states, and other unconventional physical properties. Compared with other classical TMDs, such as Mott insulator 1T–TaS_2 or superconducting 2H–NbSe_2, bulk 2H–TaSe_2 has been a canonical system and a touchstone for modeling the CDW measurement with a less complex phase diagram. In contrast to ordinary semiconductors that have only single-particle excitations, CDW can have collective excitation and carry current in a collective fashion. However, manipulating this collective condensation of these intriguing systems for device applications has not been explored. Here, the CDW-induced collective driven of non-equilibrium carriers in a field-effect transistor has been demonstrated for the sensitive photodetection at the highly-pursuit terahertz band. We show that the 2H–TaSe_2-based photodetector exhibits a fast photoresponse, as short as 14 μs, and a responsivity of over 27 V/W at room temperature. The fast response time, relative high responsivity and ease of fabrication of these devices yields a new prospect of exploring CDW condensate in TMDs with the aim of overcoming the existing limitations for a variety of practical applications at THz spectral range.

关键词： terahertz detection transition metal dichalcogenides photoconductive

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A new rabbit model of implant-related biofilm infection： development and evaluation

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Frontiers of Materials Science 2016年第1期10卷 80-89页

作者： cheng-Bing CHU Hong ZENG Ding-Xia SHEN Hui wang Ji-Fang wang Fu-Zhai CUI Beijing Chaoyang Hospital Capital Medical University Beijing 100043 China Department of Microbiology Chinese PLA General Hospital Beijing 100853 China School of Materials Science and Engineering Tsinghua University Beijing 100084 China Department of Orthopedics Chinese PLA General Hospital Beijing 100853 China

This study is to establish a rabbit model for human prosthetic joint infection and biofiim formation. Thirty-two healthy adult rabbits were randomly divided into four groups and implanted with stainless steel screws and ultra-high molecular weight polyethylene （UHMWPE） washers in the non-articular surface of the femoral lateral condyle of the right hind knees. The rabbit knee joints were inoculated with 1 mL saline containing 0, 102, 103, 104 CFU of Staphylococcus epidermidis （S. epidermidis） isolated from the patient with total knee arthroplasty （TKA） infection, respectively. On the 14th postoperative day, the UHMWPE washers from the optimal 103 CFU group were further examined. The SEM examination showed a typical biofilm construction that circular S. epidermidis were embedded in a mucous-like matrix. In addition, the LCSM examination showed that the biofilm consisted of the polysaccharide stained bright green fluorescence and S. epidermidis radiating red fluorescence. Thus, we successfully create a rabbit model for prosthetic joint infection and biofilm formation, which should be valuable for biofilm studies.

关键词： implant infection biofilm model rabbit

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Third Order Elastic Constants and Anharmonic Properties of the Zr_(41.2)Ti_(13.8)Cu_(12.5)Ni_(10.0)Be_(22.5) Bulk Metallic Glass

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Chinese Physics Letters 2001年第3期18卷 414-415页

作者： wang Ru-Ju LI Feng-Ying QIN Zhi-cheng wang Wei-Hua Institute of Physics Chinese Academy of SciencesBeijing 100080

The longitudinal velocity Vl and shear velocity Vs of ultrasonic wave propagated along arbitrary three perpendicular directions of the Zr_(41.2)Ti_(13.8)Cu_(12.5)Ni_(10.0)Be_(22.5) bulk metallic glass(Zr/Ti BMG)have been measured at ambient pressure,under hydrostatic pressure(up to 0.5 GPa)and uniaxial compression(up to 70 MPa)using the pulse echo overlap *** third order elastic constants and the Grüneisen parameter related to the anharmonic properties were derived and *** was found that the BMG with marked different microstructural characteristics comparing with the amorphous carbon exhibited different behaviours related to shear vibratory under high pressure.

关键词： Elastic harmonic hydrostatic

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Small intestinal hemangioma:Endoscopic or surgical intervention? A case report and review of literature

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World Journal of Gastrointestinal Oncology 2018年第12期10卷 516-521页

作者： ping-Fang Hu Han Chen Xiao-Hang wang Wei-Jun wang Ning Su Bin Shi Department of Gastroenterology Changzheng Hospital Second Military Medical University Department of General Surgery Hongkou Branch of Changhai Hospital Second Military Medical University Department of General Surgery Changzheng Hospital Second Military Medical University

BACKGROUND Hemangioma of the small intestine is a rare vascular malformation. Before the advent of capsule endoscopy(CE) and balloon-assisted enteroscopy(BAE), preoperative diagnosis of this disease was extremely *** SUMMARY In this study, we report a 24-year-old female with a large transmural small bowel cavernous hemangioma, which was diagnosed with CE and BAE preoperatively and removed successfully using minimally invasive surgery. Meanwhile, we perform a literature review of the studies about intestinal hemangiomas published after 2000. Literature review revealed that 91.9% of the lesions were diagnosed preoperatively by CE and/or BAE and 45.9% of them were treated endoscopically, which is a marked improvement compared to before 2000. Therefore, CE and BAE are useful modalities for the preoperative diagnosis of hemangiomas in the small intestine. CONCLUSION Endoscopic treatment of intestinal hemangioma is generally prudent and might be suitable for multiple, relatively small lesions.

关键词： Hemangioma Capsule endoscopy Balloonassisted enteroscopy Endoscopic intervention Surgery Case report

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