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OsTHA8 encodes a pentatricopeptide repeat protein required for RNA editing and splicing during rice chloroplast development

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The Crop Journal 2023年第5期11卷 1353-1367页

作者： yanwei wang Yu Duan Pengfei Ai College of Food and Biology Hebei University of Science and TechnologyShijiazhuang 050018HebeiChina

In higher plants, the chloroplast is the most important organelle for photosynthesis and for numerous essential metabolic processes in the cell. Although many genes involved in chloroplast development have been identified, the mechanisms underlying such development are not fully understood. In this study, a rice(Oryza sativa) mutant exhibiting pale green color and seedling lethality was isolated from a mutant library. The mutated gene was identified as an ortholog of THA8(thylakoid assembly 8) in Arabidopsis and maize. This gene is designated as OsTHA8 hereafter. OsTHA8 showed a typical pentatricopeptide repeat(PPR) characteristic of only four PPR motifs. Inactivation of OsTHA8 led to a deficiency in chloroplast development in the rice seedling stage. OsTHA8 was expressed mainly in young leaves and leaf *** OsTHA8 protein was localized to the chloroplast. Loss of function of OsTHA8 weakened the editing efficiency of ndhB-611/737 and rps8-182 transcripts under normal conditions. Y2H and BiFC indicated that OsTHA8 facilitates RNA editing by forming an editosome with multiple organellar RNA editing factor(OsMORF8) and thioredoxin z(OsTRXz), which function in RNA editing in rice chloroplasts. Defective OsTHA8 impaired chloroplast ribosome assembly and resulted in reduced expression of PEP-dependent genes and photosynthesis-related genes. Abnormal splicing of the chloroplast gene ycf3 was detected in ostha8. These findings reveal a synergistic regulatory mechanism of chloroplast biogenesis mediated by RNA, broaden the function of the PPR family, and shed light on the RNA editing complex in rice.

关键词： Oryza sativa L. Chloroplast biogenesis Pentatricopeptide repeat protein RNA editing RNA splicing

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Deep learning for P-wave arrival picking in earthquake early warning

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Earthquake Engineering and Engineering Vibration 2021年第2期20卷 391-402页

作者： wang yanwei Li Xiaojun wang Zifa Shi Jianping Bao Enhe College of Architecture and Civil Engineering Beijing University of TechnologyBeijing 100022China Guangxi Key Laboratory of Geomechanics and Geotechnical Engineering Guilin University of TechnologyGuilin 541004China College of Architecture and Civil Engineering Henan UniversityKaifeng 475004China Institute of Engineering Mechanics China Earthquake AdministrationHarbin 150080China China Academy of Railway Sciences China Railway CorporationBeijing 100081China

Fast and accurate P-wave arrival picking significantly affects the performance of earthquake early warning(EEW)*** P-wave picking algorithms used in EEW have encountered problems of falsely picking up noise,missing P-waves and inaccurate P-wave arrival *** address these issues,an automatic algorithm based on the convolution neural network(DPick)was developed,and trained with a moderate number of data sets of 17,717 *** to the widely used approach of the short-term average/long-term average of signal characteristic function(STA/LTA),DPick is 1.6 times less likely to detect noise as a P-wave,and 76 times less likely to miss *** terms of estimating P-wave arrival time,when the detection task is completed within 1 s,DPick′s detection occurrence is 7.4 times that of STA/LTA in the 0.05 s error band,and 1.6 times when the error band is 0.10 *** verified that the proposed method has the potential for wide applications in EEW.

关键词： P-wave arrival convolution neural network deep learning earthquake early warning

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Organo-electroreduction enables arylcarboxylation of styrenes

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Science China Chemistry 2024年第11期67卷 3825-3832页

作者： yanwei wang Qian wang Zile Zhu Weikang Qi Youai Qiu State Key Laboratory and Institute of Elemento-Organic Chemistry Frontiers Science Center for New Organic MatterHaihe Laboratory of Sustainable Chemical TransformationsCollege of ChemistryNankai UniversityTianjin 300071China

Electroreduction offers an alternative to generate high active intermediates from electrophiles(halides, alkenes, etc.) in organic synthesis. However, it still remains challenge to enable the transformations in controlled fashion for substrates with similar reduction potentials. Herein, an electroreductive arylcarboxylation of styrenes with aryl halides and CO_(2)promoted by an organomediator has been developed. The reaction exhibits the remarkable reactivity control between styrenes and aryl halides bearing very similar reduction potentials, which is enabled by the addition of a simple organic mediator. The mediated process for different kinds of aryl halides(iodides and bromides) could be achieved by simply tuning the electronic effect of the skeleton of naphthalene. This protocol displays a broad substrate scope and the extension to late-stage modification of biorelevant molecules and their derivatives showed a bright prospect. Moreover, density functional theory(DFT) calculations and mechanistic studies have been conducted and provide solid support to rationalize the selectivity and reactivity control observed.

关键词： CO_(2)utilization reductive organo-mediator carboxylation electroreduction aryl halides

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Recent Advances in Photochemical/Electrochemical Carboxylation of Olefins with CO_(2)

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Chinese Journal of Chemistry 2024年第18期42卷 2249-2266页

作者： Qian wang yanwei wang Min Liu Ganghui Chu Youai Qiu Xinjiang Laboratory of Native Medicinal and Edible Plant Resources Chemistry College of Chemistry and Environmental ScienceKashi UniversityKashiXinjiang 844000China state Key Laboratory and Institute of Elemento-Organic Chemistry Frontiers Science Centerfor New Organic MatterCollege of Chemistry Nankai University94 Weijin RoadTianjin 300071China

CO_(2) is an abundant,nontoxic,and renewable C1 feedstock in synthetic *** carboxylation of readily available olefins incorporating CO_(2) is regarded as a promising strategy to access high value-added carboxylic acids as well as CO_(2) ***,due to the thermodynamic stability and kinetic inertness of CO_(2) and the difficulty in controlling the regioselectivity,the carboxylation of olefins with CO_(2) still remains ***-type functionalization with olefins represented a powerful protocol and enabled the development of novel transformations in this *** recently,the advance of new technology,such as photoredox catalysis and the renaissance of electrochemistry in organic synthesis,offered access to unique chemical reactivities of radical precursors and provided new solutions to the functionalization of olefins.

关键词： CO_(2) Carboxylation Olefins Photocatalysis Electrocatalysis Radical Reduction Difunctionalization

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Property and Degradation Characteristics of Concrete Prepared with Aggregate Contained Montmorillonite

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Journal of Wuhan University of Technology(Materials Science) 2019年第1期34卷 127-131页

作者： wang yanwei LI Haoxin China Railway No.9 Engineering Testing CO. Ltd Key Laboratory of Advanced Civil Engineering Materials Ministry of Education Tongji University School of Materials Science and Engineering Tongji University

To evaluate the property and degradation characteristics of concrete prepared with aggregate contained montmorillonite, concretes were prepared with aggregates contained montmorillonite, and then concrete slump loss, compressive strength, electric flux, the resistance to carbonization, freezing-thawing and sulfate attack were evaluated. The results show that montmorillonite appearance alters concrete slump loss and compressive strength. But montmorillonite increases electric flux and compactness. The carbonization, freezingthawing and sulfate attack results indicate that montmorillonite enhances carbonization depth, increases mass loss after 300 cycle freezing-thawing, as well as mass loss after sulfate attack. Overall, it is adverse to the concrete resistance to the carbonization, freezing-thawing and sulfate attack.

关键词： property degradation montmorillonite slump durability

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ALM1, encoding a Fe-superoxide dismutase, is critical for rice chloroplast biogenesis and drought stress response

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The Crop Journal 2021年第5期9卷 1018-1029页

作者： yanwei wang Chen Deng Pengfei Ai Xue’an Cui Zhiguo Zhang Joint CAAS/IRRI Laboratory for Photosynthetic Enhancement Biotechnology Research Institute/National Key Facility for Genetic Resources and Gene ImprovementChinese Academy of Agricultural SciencesBeijing 100081China College of Bioscience and Bioengineering Hebei University of Science and TechnologyShijiazhuang 050000HebeiChina

Chloroplasts are the center of plant life activities including photosynthesis,growth and development,and abiotic stress *** development and biogenesis in rice have been studied in detail,but how does abiotic stress affect chloroplasts is less *** obtained an albino mutant,alm1,whose chlorophyll content was greatly *** electron microscopy showed that chloroplast development in alm1 was blocked,especially in thylakoid-like structures,which could not form *** ALM1 gene encodes a chloroplast-localized superoxide ***-length ALM1 successfully restored the non-albino phenotype,and in knockout lines,the albino phenotype *** ALM1gene is expressed mainly in young ***1 plants died as a consequence of excessive reactive oxygen accumulation after the third-leaf stage.A series of biochemical assays verified that ALM1 interacted with the OsTrxz protein,which is one of the components of plastid-encoded RNA polymerase (PEP) complexes.A western blot experiment indicated that ALM1 played an important role in stabilizing OsTrxz in *** overexpression test of ALM1 revealed that ALM1 can increase drought resistance by removing excess reactive oxygen in rice *** study suggests that ALM1 not only participates in rice chloroplast biogenesis,but also increases rice stress resistance by scavenging excess reactive oxygen.

关键词： Rice Chloroplast biogenesis PEP complex Drought stress

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Cycloaddition Reactions of Epoxides and CO_(2)Catalyzed by Bifunctional Rare-Earth Metal Complexes Bearing Amino-Bridged Tris(phenolato)Ligands

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Chinese Journal of Chemistry 2024年第14期42卷 1571-1581页

作者： Yongjie Chen yanwei wang Jun Nong Dan Yuan Yingming Yao Key Laboratory of Organic Synthesis of Jiangsu Province College of ChemistryChemical Engineering and Materials ScienceDushu Lake CampusSoochow UniversitySuzhouJiangsu215123 China

Eight zwitterionic rare earth metal complexes stabilized by amino-bridged tris(phenolato)ligands bearing quaternary ammonium side-arms were synthesized and *** complexes were used as single-component catalysts for the cycloaddition of CO_(2)and epoxides,and their catalytic activities are obviously higher than those of their binary *** studies revealed that the halide anions(Cl^(–),Br^(–),I^(–))and the metal complexes influenced the catalytic activity,and the lanthanum complex bearing iodide anion showed the highest catalytic activity for this addition reaction.A variety of mono-substituted epoxides were converted to cyclic carbonates in good to excellent yields(55%—99%)with high selectivity(>99%)at 30℃and 1 bar CO_(2),whereas internal epoxides required higher both reaction temperatures(60—120℃)and catalyst loading(2 mol%)for high *** catalyst was recyclable for four times without noticeable loss of catalytic *** on the results of kinetic studies and in℃situ IR reactions,a plausible reaction mechanism was proposed.

关键词： Bifunctional catalyst Carbon dioxide Cyclic carbonate Rare-earth metal Tris(phenolato)ligand Insertion Cycloaddition C1 building blocks

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In-situ synthesis,microstructure,and properties of NbB_(2)-NbC-Al_(2)O_(3)composite coatings by plasma spraying

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Journal of Advanced Ceramics 2022年第8期11卷 1263-1278页

作者： Xiaolong wang Yong YANG Shitong JIA yanwei wang Yuduo MA Yuhang CUI Xingyu wang Wenwei SUN Liang wang Key Lab.for New Type of Functional Materials in Hebei Province School of Materials Science and EngineeringState Key Laboratory of Reliability and Intelligence of Electrical EquipmentTianjin Key Laboratory of Materials Laminating Fabrication and Interface Control TechnologyHebei University of TechnologyTianjin 300401China Integrated Computational Materials Research Centre Shanghai Institute of CeramicsChinese Academy of SciencesShanghai 201899China

The high melting point and strong chemical bonding of NbB_(2)pose a great challenge to the preparation of high-density nanostructured NbB_(2)composite ***,we report a novel,simple,and efficient method to fabricate in-situ NbB_(2)–NbC–Al_(2)O_(3)composite coating by plasma spraying Nb_(2)O_(5)–B_(4)C–Al composite powder,aiming at realizing the higher densification and ultra-fine microstructure of NbB_(2)composite *** microstructure and properties of in-situ NbB_(2)–NbC–Al_(2)O_(3)composite coating were studied comparatively with ex-situ NbB_(2)–NbC–Al_(2)O_(3)composite coating(plasma spraying NbB_(2)–NbC–Al_(2)O_(3)composite powder).The reaction mechanism of Nb_(2)O_(5)–B_(4)C–Al composite powder in plasma jet was analyzed in *** results showed that the in-situ nanostructured NbB_(2)–NbC–Al_(2)O_(3)composite coating presented a lower porosity and superior performance including higher microhardness,toughness and wear resistance compared to the plasma sprayed ex-situ NbB_(2)–NbC–Al_(2)O_(3)coating and other boride composite *** of the in-situ NbB_(2)–NbC–Al_(2)O_(3)coating was attributed to the low melting point of Nb_(2)O_(5)–B_(4)C–Al composite powder and the exothermic effect of in-situ *** superior performance was ascribed to the density improvement and the strengthening and toughening effect of the nanosized *** in-situ reaction path could be expressed as:Nb_(2)O_(5)+Al®Nb+Al_(2)O_(3),and Nb+B_(4)C®NbB_(2)+NbC.

关键词： NbB_(2)-NbC-Al_(2)O_(3)composite coating plasma spray in-situ synthesis Nb_(2)O_(5)-B_(4)C-Al system

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Research and application of methods for effectiveness evaluation of mine cooling system

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International Journal of Mining Science and Technology 2015年第4期25卷 649-654页

作者： Guo Pingye wang yanwei Duan Mengmeng Pang Dongyang Li Nan State Key Laboratory of Geomechanics and Deep Underground Engineering School of Mechanics and Civil Engineering China University of Mining and Technology China Institute of Water Resources and Hydropower Research

Regarding the complexity and inconsistency of results in existing evaluation methods of mine cooling system, this paper clarifies the advantages, disadvantages and application of various mine cooling sys- tems through principle analysis, and divides all the cooling systems into air-cooling, ice-cooling and water-cooling according to the transportation of cold energy. On this basis, the paper proposes a simple and efficient evaluation method for mine cooling system. The first index of this method is the air temper- ature at point C which is 15 m away from the return wind corner at working face. A cooling system will be judged ineligible if the air temperature at point C is above 30 ℃ during operation, because in this case, the combustible gases in coal will sharply overflow, inducing gas incidents. Based on the preliminary judg- ment of the first index, another two evaluation indexes are proposed based on the cooling ability and dehumidification of an airflow volume of 1000 m3/min at point C to evaluate the investment and opera- tion cost of mine cooling system. This evaluation method has already been successfully applied in the cooling system design of Zhangshuanglou coal mine.

关键词： Cooling system Effectiveness Evaluation Index system

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Function identification of miR159a,a positive regulator during poplar resistance to drought stress

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Horticulture Research 2023年第12期10卷 120-136页

作者： Tiantian Fu Chun wang Yuzhang Yang Xiaoqian Yang Jing wang Lichun Zhang Zeqi wang yanwei wang State Key Laboratory of Tree Genetics and Breeding National Engineering Research Center of Tree Breeding and Ecological RestorationKey Laboratory of Genetics and Breeding in Forest Trees and Ornamental PlantsMinistry of EducationThe Tree and Ornamental Plant Breeding and Biotechnology Laboratory of National Forestry and Grassland AdministrationCollege of Biological Sciences and BiotechnologyBeijing Forestry UniversityBeijing 100083China

Drought seriously affects the growth and development of ***159 is a highly conserved and abundant microRNA family that plays a crucial role in plant growth and stress ***,studies of its function in woody plants are still ***,the expression of miR159a was significantly upregulated after drought treatment in poplar,and the overexpression of miR159a(OX159a)significantly reduced the open area of the stomata and improved water-use efficiency in *** drought treatment,OX159a lines had better scavenging ability of reactive oxygen species and damage of the membrane system was less than that in wild-type *** was the target gene of miR159a,as verified by psRNATarget prediction,RT-qPCR,degradome sequencing,and 5′rapid amplification of cDNA ends(5′RACE).Additionally,miR159a-short tandem target mimic suppression(STTM)poplar lines showed increased sensitivity to drought *** analysis comparing OX159a lines with wild-type lines revealed upregulation of a series of genes related to response to water deprivation and metabolite ***,drought-responsive miR172d and miR398 were significantly upregulated and downregulated respectively in OX159a *** investigation demonstrated that miR159a played a key role in the tolerance of poplar to drought by reducing stomata open area,increasing the number and total area of xylem vessels,and enhancing water-use efficiency,and provided new insights into the role of plant miR159a and crucial candidate genes for the molecular breeding of trees with tolerance to drought stress.

关键词： poplar breeding drought

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