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山羊源贝氏柯克斯体抗体间接ELISA检测方法的建立与应用

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动物医学进展 2024年第4期45卷 21-26页

作者：张锐铮于皓同王凯茸张琪许信刚西北农林科技大学动物医学院陕西杨凌712100

为建立山羊源贝氏柯克斯体(Coxiella burnetii)抗体间接ELISA检测方法,对贝氏柯克斯体的com1基因进行克隆及原核表达,以com1纯化蛋白作为抗原,建立间接ELISA检测方法,并对临床收集的血清样本进行检测。结果显示,重组蛋白大小为27 ku,经W... 详细信息

为建立山羊源贝氏柯克斯体(Coxiella burnetii)抗体间接ELISA检测方法,对贝氏柯克斯体的com1基因进行克隆及原核表达,以com1纯化蛋白作为抗原,建立间接ELISA检测方法,并对临床收集的血清样本进行检测。结果显示,重组蛋白大小为27 ku,经Western blot鉴定com1蛋白反应原性良好;ELISA检测方法抗原包被量为4μg/mL,血清的稀释倍数为1∶100,酶标二抗稀释倍数为1∶2500,阴阳临界值为0.278。用该方法对流产衣原体、山羊支原体、牛支原体阳性血清进行检测,均为阴性;当***阳性血清稀释至2048倍时检测结果仍为阳性;批内、批间变异系数均小于10%。用该方法对陕西省部分羊场收集到的307份血清样本进行检测,样本阳性率为9.12%。成功建立了一种检测贝氏柯克斯体抗体的间接ELISA检测方法,这为***引起的Q热的实验室诊断和流行病学调查提供了技术支持。

关键词：贝氏柯克斯体 com1蛋白酶联免疫吸附试验抗体检测

Effects of Long-Term Fertilization on the Distribution of Carbon, Nitrogen and Phosphorus in Water-Stable Aggregates in Paddy Soil

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Agricultural Sciences in China 2011年第12期10卷 1932-1940页

作者： wang Wei CHEN Wei-cai wang kai-rong XIE Xiao-li YIN Chun-mei CHEN An-lei Key Laboratory of Agro-Ecological Processes in Subtropical Region Institute of Subtropical Agriculture Chinese Academy of Sciences Changsha 410125 P.R.China Institute of Agriculture Ecological and Environmental Health Qingdao Agricultural University Qingdao 266109 P.R.China Graduate University of Chinese Academy of Sciences Beijing 100049 P.R.China

We investigated the size distribution of water-stable aggregates and the soil carbon, nitrogen and phosphorus concentration over aggregate size fractions based on a long-term （1990-2006） fertilization experiment in a reddish paddy soil. The results showed that the largest water-stable aggregate （WSA）（〉5 mm） and the smallest WSA （〈0.25 mm） took up the first largest proportion （38.3%） and the second largest proportion （23.3%）, respectively. Application of organic materials increased the proportion of the large WSA （〉2 mm） and decreased the proportion of the small WSA （〈1 ram）, resulting in an increase in the mean weight diameter of WSA, whereas application of chemical fertilizer had little effect. Application of organic materials, especially combined with chemical fertilizers, increased total carbon, nitrogen and phosphorus concentrations in all sizes of WSA, and total carbon, nitrogen and phosphorus were prone to concentrate in the large WSA. Further more, application of organic materials improved the supply effectiveness of available phosphorus, whereas had little influence on the labile carbon in WSA. Application of chemical fertilizers improved concentrations of total and available phosphorus in all sizes of WSA, whereas had little influence on total carbon and nitrogen contents. Economical fertilization model maintained the soil fertility when compared with full dose of chemical fertilizers, indicating that using organic materials could reduce chemical fertilizers by about one third.

关键词： distribution fertilization nutrient water-stable aggregate

羊贝氏柯克斯体TaqMan荧光定量PCR检测方法的建立及应用

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动物医学进展 2024年第7期45卷 6-10页

作者：张锐铮于皓同王凯茸张琪张淑霞许信刚西北农林科技大学动物医学院陕西杨凌712100

为建立检测贝氏柯克斯体(Coxiella burnetii)实时荧光定量PCR方法,根据GenBank上收录的*** RSA439株com1基因序列(CP040059.1)保守区设计特异性引物和探针,并构建重组质粒及优化反应条件,以期建立一种检测贝氏柯克斯体的Taq Man荧光定量... 详细信息

为建立检测贝氏柯克斯体(Coxiella burnetii)实时荧光定量PCR方法,根据GenBank上收录的*** RSA439株com1基因序列(CP040059.1)保守区设计特异性引物和探针,并构建重组质粒及优化反应条件,以期建立一种检测贝氏柯克斯体的Taq Man荧光定量PCR方法。结果显示,该方法所建立标准曲线线性关系良好;敏感性试验结果显示,最低检出限为2.45×10^(2) copies/μL,与PCR最低检出限为2.45×10^(4) copies/μL相比较,灵敏度提高了100倍;对羊支原体、羊流产衣原体、羊布鲁氏菌等均无交叉反应,批内和批间重复性试验结果稳定,变异系数均小于3%,说明特异性、重复性好;利用该方法对收集的126份样品进行检测,结果显示样品阳性率为9.52%,常规PCR检测阳性率为7.14%,符合率为90.47%。该方法可为贝氏柯克斯体临床上快速检测及防控提供技术支持。

关键词：贝氏柯克斯 Taq Man荧光定量PCR 检测方法

基于gE蛋白的羊伪狂犬病病毒间接ELISA的建立与应用

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动物医学进展 2024年第3期45卷 28-33页

作者：刘广阔吴发兴于皓同王凯茸张锐铮张琪许信刚西北农林科技大学动物医学院陕西杨凌712100 中国动物卫生与流行病学中心国家动物血清库山东青岛266114

为建立羊源伪狂犬病病毒(Pseudorabies virus,PRV)抗体的检测方法,从PRV中对gE基因进行克隆,并构建重组载体对gE蛋白进行原核表达,以重组gE蛋白为包被抗原,建立PRV抗体间接ELISA检测方法,并进行临床样本检测。结果显示,重组gE蛋白大小为... 详细信息

为建立羊源伪狂犬病病毒(Pseudorabies virus,PRV)抗体的检测方法,从PRV中对gE基因进行克隆,并构建重组载体对gE蛋白进行原核表达,以重组gE蛋白为包被抗原,建立PRV抗体间接ELISA检测方法,并进行临床样本检测。结果显示,重组gE蛋白大小为42 ku,以包涵体形式表达;Western blot检测表明重组蛋白具有良好的反应原性;重组蛋白作为包被抗原的最佳工作浓度为1μg/mL,待检血清100倍稀释,以HRP标记的兔抗山羊IgG 10000倍稀释作为二抗;检测临床样本时判定阴阳性的临界值为0.284;灵敏性试验结果显示,羊PRV阳性血清稀释2048倍时检测结果仍为阳性;批内变异系数(2.45%~5.00%)与批间变异系数(2.55%~7.41%)均小于10%;采用建立的方法检测其他常见羊源病毒阳性血清结果均为阴性;对收集的376份临床羊血清进行检测,PRV阳性率为10.6%。建立的间接ELISA检测方法可用于羊源样本中伪狂犬病病毒抗体的检测,该方法的建立为羊场PRV抗体检测提供了技术支持。

关键词：羊伪狂犬病伪狂犬病病毒 gE蛋白间接ELISA

基于VP6蛋白的牛轮状病毒抗体间接ELISA检测方法的建立与应用

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动物医学进展 2024年第1期45卷 1-6页

作者：刘广阔邹敏吴发兴于皓同王凯茸张锐铮张琪许信刚西北农林科技大学动物医学院陕西杨凌712100 青岛易邦生物工程有限公司/科技部动物基因工程疫苗国家重点实验室山东青岛266114 中国动物卫生与流行病学中心/国家动物血清库山东青岛266114

为了建立牛轮状病毒(BRV)血清抗体的检测方法,从病料中克隆牛轮状病毒VP6基因,构建其表达载体,利用原核表达技术表达重组VP6蛋白,建立牛轮状病毒血清抗体间接ELISA检测方法。结果显示,重组VP6蛋白大小为40 ku,以包涵体形式表达,Western ... 详细信息

为了建立牛轮状病毒(BRV)血清抗体的检测方法,从病料中克隆牛轮状病毒VP6基因,构建其表达载体,利用原核表达技术表达重组VP6蛋白,建立牛轮状病毒血清抗体间接ELISA检测方法。结果显示,重组VP6蛋白大小为40 ku,以包涵体形式表达,Western blot证实重组VP6蛋白有良好的反应原性;以4μg/mL浓度的抗原包被酶标板,一抗血清50倍稀释,酶标二抗稀释10000倍稀释,为最佳工作条件,阴阳临界值为0.233,表明该方法具有良好的特异性和敏感性。建立的检测BRV抗体的间接ELISA可用于临床BRV感染的检测。

关键词：牛轮状病毒 VP6蛋白原核表达间接酶联免疫吸附试验

Phosphorus Transfer and Distribution in a Soybean-Citrus Intercropping System

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Pedosphere 2006年第4期16卷 435-443页

作者： ZHOU Wei-Jun wang kai-rong ZHANG Yang-Zhu YIN Li-Chu LI He-Song College of Resources and Environment Hunan Agricultural University Changsha 410128 （China） Institute of Subtropical Agriculture Chinese Academy of Sciences Changsha 4 10125 （China）

A mini-plot field experiment was conducted on a loamy clay Oxisol to compare and evaluate P absorption and transfer in plant organs and P movement in soil profile at three P application depths under the soybean-citrus intercropping versus the monoculture using a ^32p tracer technique. Total P absorption （Pt） by soybean and P accumulation （Pa） in soybean organs decreased significantly （P 〈 0.05） under the intercropping in contrast to the monoculture. With intercropping, when ^32p was applied in topsoil （15 cm soil layer）, total ^32p absorption （^32pt） in soybeans was significantly lower （P 〈 0.05）, but when ^32p was applied to deeper soil layers （35 or 55 cm soil layer）, ^32pt in soybeans was significantly greater （P 〈 0.05）. The percentage of P in leaves to total P （Pa/Pt） and 32p in leaves to total ^32p （^32pa/^32pt） for soybean were ≥ 25% and those of root ≥ 12%. When P was applied ia topsoil and 55 cm soil layer, no significant differences were found between intercropping and monoculture for Pt of citrus. The P absorbed by citrus was transferred rapidly to the growing organs of aboveground during the experiment, and the speed of transferring to the growing organs slowed when P was applied to the deeper soil layers. In intercropping, P mobility was heightened in the soil profile, and P in deeper soil layers moved up to topsoil more rapidly.

关键词： agroforestry ecosystem citrus monoculture, ^32p fate soybean

The role of tazarotene-induced gene 1 in carcinogenesis:is it a tumor suppressor gene or an oncogene?

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BIOCELL 2024年第9期48卷 1285-1297页

作者： CHUN-HUA wang LU-kai wang rong-YAUN SHYU FU-MING TSAI Department of Dermatology Taipei Tzu Chi HospitalBuddhist Tzu Chi Medical FoundationNew Taipei City231TaiwanChina School of Medicine Tzu Chi UniversityHualien970TaiwanChina Department of Life Sciences Ministry of Science and TechnologyTaipei106TaiwanChina Department of Internal Medicine Taipei Tzu Chi HospitalThe Buddhist Tzu Chi Medical FoundationNew Taipei City231TaiwanChina Department of Research Taipei Tzu Chi HospitalBuddhist Tzu Chi Medical FoundationNew Taipei City231TaiwanChina

Tazarotene-induced gene 1(TIG1)is induced by a derivative of vitamin A and is known to regulate many important biological processes and control the development of ***1 is widely expressed in various tissues;yet in many cancer tissues,it is not expressed because of the methylation of its ***,the expression of TIG1 in cancer cells inhibits their growth and invasion,suggesting that TIG1 acts as a tumor suppressor ***,in some cancers,poor prognosis is associated with TIG1 expression,indicating its protumor growth characteristics,especially in promoting the invasion of inflammatory breast cancer *** review comprehensively summarizes the roles of the TIG1 gene in cancer development and details the mechanisms through which TIG1 regulates cancer development,with the aim of understanding its various roles in cancer development.

关键词： Tazarotene-induced gene 1 Retinoic acid receptor responder protein 1 Tumor suppressor gene Oncogene

The response of Martian photoelectron boundary to the 2018 global dust storm

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Science China Earth Sciences 2024年第9期67卷 2772-2782页

作者： Yuqi wang Jun CUI Yong WEI Zhaopeng WU kai FAN Zhaojin rong Fei HE Yutian CAO Jiawei GAO Key Laboratory of Earth and Planetary Physics Institute of Geology and GeophysicsChinese Academy of SciencesBeijing 100029China College of Earth and Planetary Sciences University of Chinese Academy of SciencesBeijing 100049China Planetary Environmental and Astrobiological Research Laboratory(PEARL) School of Atmospheric SciencesSun Yat-sen UniversityZhuhai 519082China Center for Excellence in Comparative Planetology Chinese Academy of SciencesHefei 230026China

Extensive research efforts have revealed that the Martian dust storms can perturb the upper atmospheric condition and as a consequence,enhance plasma density and photoelectron flux in the ***,previous observational studies of the Martian dust storm impacts have been restricted to regions below 400 km,which limits our understanding of the Martian dust storm effects in the upper ionosphere and ***,based on the suprathermal electron measurements made by the Solar Wind Electron Analyzer onboard the Mars Atmosphere and Volatile Evolution,we identify with an automatic procedure the occurrences of all photoelectron boundary(PEB)crossings at solar zenith angle below 120°(with a dust-free median altitude of about 600 km).Using the dayside PEB as a proxy of the upper ionospheric and magnetospheric condition,we analyze the variations of the PEB altitude during the 2018 global dust storm(GDS)of Mars Year 34(MY34)and compare them with the period in MY33 when there was no global dust *** conclude that the column dust optical depth(CDOD)emerges as one of the main driving factors for PEB altitude variations during the *** analysis implies that the GDS can affect the Martian upper atmosphere and ionosphere over considerable distances and extended time scales.

关键词： Martian global dust storm Martian photoelectron boundary Upper ionosphere

Adaptive state-constrained/model-free iterative sliding mode control for aerial robot trajectory tracking

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Applied Mathematics and Mechanics(English Edition) 2024年第4期45卷 603-618页

作者： Chen AN Jiaxi ZHOU kai wang College of Mechanical and Vehicle Engineering Hunan UniversityChangsha 410082China Department of Mechanical Engineering The Hong Kong Polytechnic UniversityHong KongChina

This paper develops a novel hierarchical control strategy for improving the trajectory tracking capability of aerial robots under parameter *** hierarchical control strategy is composed of an adaptive sliding mode controller and a model-free iterative sliding mode controller(MFISMC).A position controller is designed based on adaptive sliding mode control(SMC)to safely drive the aerial robot and ensure fast state convergence under external ***,the MFISMC acts as an attitude controller to estimate the unmodeled dynamics without detailed knowledge of aerial ***,the adaption laws are derived with the Lyapunov theory to guarantee the asymptotic tracking of the system ***,to demonstrate the performance and robustness of the proposed control strategy,numerical simulations are carried out,which are also compared with other conventional strategies,such as proportional-integralderivative(PID),backstepping(BS),and *** simulation results indicate that the proposed hierarchical control strategy can fulfill zero steady-state error and achieve faster convergence compared with conventional strategies.

关键词： aerial robot hierarchical control strategy model-free iterative sliding mode controller(MFISMC) trajectory tracking reinforcement learning