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Changes of Reactive Oxygen Species and Related Enzymes in Mitochondrial Respiration During Storage of Harvested Peach Fruits

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Agricultural Sciences in China 2011年第1期10卷 149-158页

作者： KAN juan wang hong-mei JIN Chang-hai College of Food Science and Engineering Yangzhou University Yangzhou 225127 P.R.China

Peach fruits [Prumus persica （L.） Batsch, cv. Yuhuasanhao] were used as materials to investigate the changes of reactive oxygen species （ROS） and related enzymes in mitochondria respiration during storage and then their influence on senescence of harvested Peach fruits was studied. The results showed that low temperature （5℃） strongly inhibited the reduction of firmness and the increase in respiration rate. During storage at ambient temperature （20℃）, ROS had a cumulative process while malondialdehye （MDA） content continued to increase in associated with enhanced membrane lipid peroxidation. Lipoxygenase （LOX） activity was strongly inhibited under the low temperature condition. The activities of succinic dehydrogenase （SDH）, cytochrome C oxidase （CCO）, and Ca^2＋-ATPase declined to a certain extent at ambient temperature, while they showed higher activities at low temperature, which may be related to lower membrane lipid peroxidation at low temperature. Higher Ca^2＋ content at ambient temperature may be responsible for impairment of mitochondrial function, thus, leading to fruit senescence. The results showed that under low temperature condition, the low accumulation of ROS and the low level of membrane lipid peroxidation could maintain the function of mitochondria that would help to delay the senescence of peach fruits. These suggested a close relationship existed between ROS metabolism and mitochondrial respiration. It can be inferred that the low temperature helps to delay senescence of peach fruits via suppression of ROS and related enzymes, maintain better homeostasis of Ca^2＋ in mitochondria and thus better mitochondrial functions.

关键词： peach fruits ripening active oxygen enzymes related to mitochondria respiratory metabolism

A Silicon Tunnel Field-Effect Transistor with an In Situ Doped Single Crystalline Ge Source for Achieving Sub-60 mV/decade Subthreshold Swing

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Chinese Physics Letters 2013年第8期30卷 209-211页

作者： LIU Yan wang hong-juan YAN Jing HAN Gen-Quan Key Laboratory of Optoelectronic Technology and Systems of the Education Ministry of China College of Optoelectronic EngineeringChongqing UniversityChongqing 400044

We report the demonstration of an n-channel lateral Si tunnel field-effect transistor(TFET)with a single crystalline Ge source fabricated using the gate-last *** p Ge source was in situ doped and grown at 320℃.An abrupt interface between Ge source and Si channel with type-II band alignment and a steep source doping profile(~1.5 nm/decade)formed the tunneling *** allows the realization of a TFET with a steep subthreshold swing of 49 mV/decade at room temperature and an ION/IOFF ratio of 107.

关键词： channel Tunnel realization

Highly Sensitive and Selective Amperometric Sensor for Iodate Based on 9,10-Phenanthrenequinone Derived Graphene

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Chemical Research in Chinese Universities 2013年第1期29卷 132-138页

作者： wang hong-juan CHENG Na-na YANG Xiao-yang LI Xiao-meng ZHU Lian-de Faculty of Chemistry Northeast Normal University Changchun 130024 P. R. China

We reported on a new amperometric sensor for the sensitive and selective determination of iodate in table salt. The iodate sensor was constructed by the integration of a novel nanocomposite which was made from 9,10-phenanthrenequinone（PQ） and graphene（GP） with a glassy carbon electrode（GCE）. The synthesized graphene and the nanocomposite were well characterized by X-ray diffraction（XRD）, transmission electron microscopy（TEM）, Fourier transform infrared（FTIR） spectroscopy and Raman spectroscopy. We fully studied the electrochemical beha- vior and kinetic characteristics of the PQ/GP nanocomposite at GCE. The PQ/GP electrode shows a good electrochemical catalytic activity towards the reduction of iodate, which makes itself a sensitive and selective electrochemical sensor for iodate. The iodate sensor displays a high sensitivity（1.04 ***.L-1）, a low detection limit（1.0X 10-8 mol/L）, a rapid response（less than 2 s）, and a broad linear range（from 5.0X 10-8 mol/L to 6.0X 10-3 mol/L ）. In addition, the sensor is interference free. The practical application of the proposed sensor was tested by the detection of iodate in table salt.

关键词： 9,10-Phenanthrenequinone Graphene Amperometric sensor Iodate

K-19 mRNA RT-PCR in detecting micrometastasis in regional lymph nodes of gastric cancer

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World Journal of Gastroenterology 2006年第32期12卷 5219-5222页

作者： Jian Suo Quan wang hong-juan Jin hong Li Hang Zhao Department of General Surgery First Hospital Changchun 130021 Jilin Province China

AIM： To investigate the value and prospect of RT-PCR in detecting micrometastasis in regional lymph nodes of gastric cancer. METHODS： Histopathology was used and K19 mRNA expression was detected by RT-PCR in tumor tissues and lymph nodes from gastric cancer patients undergoing radical resection of gastric carcinoma. RESULTS： K19 mRNA was expressed in all tumor specimens of 30 cases; of the 126 lymph nodes, 26 were histopathologically positive （20.6%）, and 42 positive （33.3%） by RT-PCR. Amplification fragments of 460 and 540 bp were shown in all the tumor tissues and metastatic lymph nodes after K19 and β-actin RT-PCR, while only a 540 bp fragment appeared in the lymph nodes of non-tumor patients. CONCLUSION： K19 mRNA RT-PCR is sensitive and specific in testing micrometastasis in regional lymph nodes of gastric cancer, and it is superior to routine histopathology.

关键词： K-19 mRNA RT-PCR Micrometastasis Gastric cancer

Identification of novel and differentially expressed micro RNAs in ovine ovary and testis tissues using Solexa sequencing and bioinformatics

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Journal of Integrative Agriculture 2015年第8期14卷 1604-1616页

作者： CHANG Wei-hua ZHANG Yong CHENG Zhang-rui ZHAO Xing-xu wang juan-hong MA You-ji HU Jun-jie ZHANG Quan-wei College of Veterinary Medicine Gansu Agricultural University College of Animal Science Tarim University Royal Veterinary College London University College of Animal Science and Technology Gansu Agricultural University

Micro RNAs（mi RNAs） are small, single stranded, non-coding RNA molecules, about 19–25 nucleotides in length, which regulate the development and functions of reproductive system of *** discover novel mi RNAs and identify the differential expression of them in ovine ovary and testis tissues, the study constructed two libraries by using next-generation sequencing technologies（Solexa high-throughput sequencing technique）.As a result, 9 321 775 and 9 511 538 clean reads were obtained from the ovary and testis separately, which included 130 562（90 genes of ovary） and 56 272（85 genes of testis） of known mi RNAs and 486 potential novel mi RNAs *** this study, a total of 65 conserved mi RNAs were significantly differentially expressed（P〈0.01） between the two *** them, 28 mi RNAs were up-regulated and 3 mi RNAs were down-regulated on ovary compared with *** addition, the known mi RNAs with the highest expression level（5 mi RNAs） and 30 novel mi RNAs with the functions related to reproduction were validated using the real-time quantitative ***, the gene ontology（GO） annotation and Kyoto encyclopedia of genes and genomes（KEGG） pathway analysis showed that differentially expressed mi RNAs were involved in ovary and testis physiology, including signal transduction, gonad development, sex differentiation, gematogenesis, fertilization and embryo *** results will be helpful to facilitate studies on the regulation of mi RNAs during ruminant reproduction.

关键词： microRNA ovary testis solexa sequencing qRT-PCR

Changes of Reactive Oxygen Species and Related Enzymes in Mitochondria Respiratory Metabolism During the Ripening of Peach Fruit

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Agricultural Sciences in China 2010年第1期9卷 138-146页

作者： KAN juan,wang hong-mei,JIN Chang-hai and XIE Hai-yan College of Food Science and Engineering,Yangzhou University,Yangzhou 225127,P.R.China [a] College of Food Science and Engineering Yangzhou University Yangzhou 225127 P.R. China

The fruits of peach cultivar Yuhua 3 were used as materials to investigate the changes of active oxygen and related enzymes in mitochondria respiratory metabolism during ripening of peach fruit, involving their influence on the proceeding of peach fruit senescence. The results showed that the large decrease in firmness occurred between maturity II and IV. The decrease in firmness coincided with an increase in respiratory intensity. Obvious peaks of respiratory intensity lagging to the rapid change of fruit firmness could be shown during peach ripening. Reactive oxygen species （ROS） had a cumulative process and positively correlated with respiratory intensity. During peach ripening, the content of Ca^2＋ increased, the activities of succinic dehydrogenase （SDH）, cytochrome C oxidase （CCO）, H＋-ATPase, and Ca^2＋-ATPase decreased varying in different degree at the later step of ripening. These suggested a close relationship existed between ROS metabolism and mitochondrial respiration, namely, both ROS metabolism and mitochondrial respiration probably played important roles in ripening and senescing of peach fruit.

关键词： peach fruit ripening reactive oxygen species enzymes related to mitochondria respiratory metabolism

Inhibiting water evaporation of sandy soil by the soil particles modified with Japanese wax

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Journal of Forestry Research 2009年第1期20卷 59-62页

作者： ZHANG Zeng-Zhi wang hong-juan Li Cui-Lan Research institute of Ecological and Functional Material China University of Mining and Technology Beijing 100083

This study was conducted to resolve the problems of water conservation of sandy soil in desertification areas. The surface of soil particles was modified by molecules of natural Japanese wax through some specially screened surfactant. The modified particles were then well sprayed onto the sand, which was placed in an artificial climate box with simulating desert environment, to form a soil film with effect of suppressing water and gas-permeability. Structure of soil film was analyzed by means of X-ray diftYaction （XRD） and infrared spectrometry （IR）. And its mechanism of water inhibition was illustrated with DSC and TG curves. Its influence on grass-planting was tested through the instruments of water detector. The results show that sorbitol anhydride stearate （ Span 80） could well disperse the Japanese wax and make it combine with the clay which is also dispersed. The pores among soil particles grew smaller and turned from hydrophilic into hydrophobic, in which way resistance to water penetrating through the film was increased. Experimental grass grows normally on sandy soil with the soil film in the artificial desert climate box, indicating that the soil particles modified with Japanese wax is an effective method to inhibit water evaporation.

关键词： Japanese wax surfactant desert soil hydrophobic soil film

The preparation and moisture-holding capacity of soil-based hydrophobic film with Japanese wax

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材料科学与工程（中英文版） 2008年第12期2卷 1-6页

作者： ZHANG Zeng-zhi wang hong-juan Li Cui-lan Research Institute of Ecological and Functional Material China University of Mining and Technology. Beijing 100083 China

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抗战时期陕甘宁边区的食盐走私与缉私

河北学刊 2019年第5期39卷 125-132页

作者：汪红娟陕西师范大学历史文化学院西安外国语大学马克思主义学院

抗战时期,食盐在陕甘宁边区经济中占有重要地位。'皖南事变'前,边区财政来源主要是外援,政府只征少量的盐税,故食盐走私并不严重;'皖南事变'后,外援断绝,随着边区政府对盐税的倚重及管理的加强,食盐走私逐渐泛滥。走私形式花样百出,不... 详细信息

抗战时期,食盐在陕甘宁边区经济中占有重要地位。'皖南事变'前,边区财政来源主要是外援,政府只征少量的盐税,故食盐走私并不严重;'皖南事变'后,外援断绝,随着边区政府对盐税的倚重及管理的加强,食盐走私逐渐泛滥。走私形式花样百出,不一而足,走私原因也是多方面的,其对边区所造成的政治经济影响不可忽视。鉴于此,边区政府采取多种措施治理打击食盐走私,诸如健全缉私机构,颁布相应的法规制度,发动群众力量缉私等。在边区政府的治理及严厉打击下,食盐缉私取得了一定成效,但并未销声匿迹。

关键词：陕甘宁边区食盐走私原因影响缉私