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Rapid Screening of chloramphenicol in Turbot with photobacterium phosphoreum DB

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High Technology Letters 2009年第2期15卷 214-219页

作者：朱兰兰 wang jingxue Lin Hong wang Yaqun wang Jing Mei Cexia Food Science and Engineering Department Ocean University of China Qingdao 266003 P.R. China Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Qingdao 266071 P.R. China

A new,simple,rapid microbial assay was put forward for the detection of chloramphenicol(CAP)residues in Turbot muscles using luminescent bacteria DB(Photobacterium phosphoreum)as test *** the luminescence inhibition of DB,the concentrations of CAP were *** spiked inTurbot muscle tissues ranging from 0.5 to 100μg/kg was extracted using ethyl acetate and *** had a good recovery of 93.69% with a minimum detection limit of 0.65μg/*** can be per-formed within 30min following simple *** method therefore proved to be advantageous overchromatographic procedures as it was inexpensive,quite sensitive and can be adopted for rapid screeningof CAP in Turbot tissues.

关键词： chloramphenicol Photobacterium phosphoreum microbial assay turbot

Microenvironment Modulation in Metal−Organic Framework-Based Catalysis

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Accounts of Materials Research 2021年第5期2卷 327-339页

作者： Long Jiao jingxue wang Hai-Long Jiang Hefei National Laboratory for Physical Sciences at the Microscale CAS Key Laboratory of Soft Matter ChemistryDepartment of ChemistryUniversity of Science and Technology of ChinaHefeiAnhui 230026P.R.China

CONSPECTUS:The fine design and regulation of catalysts play critical roles in the development of *** microenvironment,which gives rise to unique spatial structures and electronic properties around catalytic sites,has been proven to dramatically regulate catalytic behavior in enzymes and homogeneous ***,understanding the microenvironment modulation(MEM)of catalytic sites remains challenging and very limited in heterogeneous catalysis mainly due to the lack of structural precision and/or tailorability of traditional solid *** diverse materials,metal−organic frameworks(MOFs),a class of porous crystalline solids,have been intensively studied as heterogeneous catalysts in recent *** atomically precise and well tunable structures of MOFs make them an ideal platform for rationally regulating the microenvironment surrounding catalytic ***,their well-defined structures hold great promise for elucidating how the microenvironment modulation affects the resulting catalytic ***,the investigations of accurate control over the microenvironment of catalytic sites in MOFs for modulated catalysis are still very ***,it is of great importance to summarize the related results and provide in-depth insights into microenvironment modulation in MOF-based catalysis,accelerating the future development of this emerging research *** this Account,we have presented a summary of our recent attempts to optimize the catalytic performance of MOF-based materials via microenvironment *** view of the unique component and structural advantages of MOFs,we deliver the general fundamentals for rational control over the microenvironment in MOF-based ***,the great opportunities brought about by MOFs for accurate control over microenvironment engineering,including the origin of abundant active sites,flexible regulation strategies,and well-defined structure,are introduced in *** the ne

关键词： catalysis rational precise

Generation of Hierarchical Pores in Metal-Organic Frameworks by Introducing Rigid Modulator

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CCS Chemistry 2022年第12期4卷 3705-3714页

作者： Zhiwen Ai Long Jiao jingxue wang Hai-Long Jiang Department of Chemistry University of Science and Technology of ChinaHefeiAnhui 230026

The fabrication of hierarchically porous metal-organic frameworks(HP-MOFs)that combine tunable mesopore sizes with high stability is highly desired but remains a technical ***,a facile and versatile“rigid modular-assisted defect formation”strategy has been developed to transform microporous MOFs into their corresponding *** controlling the modulator dose and acid amount,the pore size and mesopore percentage can be finely *** on the hierarchical pores,the mass transfer of molecules with large sizes is significantly *** a result,the representative HP-UiO-66-NH_(2)-OTf,decorated with a trifluoromethanesulfonate(OTf)group to enhance Lewis acidity,exhibits excellent activity and selectivity in tandem catalysis,far superior to pristine UiO-66-NH_(2).This work provides a novel strategy to the general synthesis of stable HP-MOFs.

关键词： hierarchically porous materials metal-organic frameworks rigid modulators heterogeneous catalysis stability

Necrotic enteritis challenge regulates peroxisome proliferator-1 activated receptors signaling and β-oxidation pathways in broiler chickens

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Animal Nutrition 2021年第1期7卷 239-251页

作者： Kosar Gharib-Naseri Sara de Las Heras-Saldana Sarbast Kheravii Lihong Qin jingxue wang Shu-Biao Wu School of Environment and Rural Science University of New EnglandArmidaleNSW2351Australia Animal Science and Husbandary Branch Jilin Academy of Agricultural SciencesGongzhulingJilin136100China College of Life Sciences Shanxi UniversityTaiyuanShanxi030006China

Necrotic enteritis(NE)is an important enteric disease in poultry and has become a major concern in poultry production in the post-antibiotic *** infection with NE can damage the intestinal mucosa of the birds leading to impaired health and,thus,*** gain a better understanding of how NE impacts the gut function of infected broilers,global mRNA sequencing(RNA-seq)was performed in the jejunum tissue of NE challenged and non-challenged broilers to identify the pathways and genes affected by this ***,to induce NE,birds in the challenge group were inoculated with 1 mL of Eimeria species on day 9 followed by 1 mL of approximately 108 CFU/mL of a NetB producing Clostridium per-fringens on days 14 and *** day 16,2 birds in each treatment were randomly selected and euthanized and the whole intestinal tract was evaluated for lesion *** tissue samples from one of the euthanized birds of each replicate(n=4)was used for histology,and the jejunum tissue for RNA ***-seq analysis was performed with an Illumina RNA HiSeq 2000 *** differ-entially expressed genes(DEG)were identified and functional analysis was performed in DAVID to find protein-protein interactions(PPI).At a false discovery rate threshold<0.05,a total of 377 DEG(207 upregulated and 170 downregulated)DEG were *** enrichment analysis revealed that DEG were considerably enriched in peroxisome proliferator-activated receptors(PPAR)signaling(P<0.01)andβ-oxidation pathways(P<0.05).The DEG were mostly related to fatty acid metabolism and degradation(cluster of differentiation 36[CD36],acyl-CoA synthetase bubblegum family member-1[ACSBG1],fatty acid-binding protein-1 and-2[FABP1]and[FABP2];and acyl-coenzyme A synthetase-1[ACSL1]),bile acid production and transportation(acyl-CoA oxidase-2[ACOX2],apical sodium-bile acid transporter[ASBT])and essential genes in the immune system(interferon-,[IFN-γ],LCK proto-oncogene,Src family tyrosine kinase[LCK],zeta

关键词： Necrotic enteritis Clostridium perfringens Transcriptome Fatty acid metabolism Broiler Challenge

Ultrasound-activated in situ click chemistry to trigger autophagosome tracking for enhanced autophagy blockade and synergistic cancer therapy

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Aggregate 2024年第4期5卷 386-398页

作者： Weixi Jiang jingxue wang Li Chen Xiaoling Qiu Chier Du Hongjin An Xun Guo Xiaoting wang Junrui wang Pan Li Zhigang wang Haitao Ran Zhiyi Zhou Xiaoyuan Chen Jingjing Zhang Jianli Ren Department of Ultrasound and Chongqing Key Laboratory of Ultrasound Molecular Imaging the Second Affiliated Hospital of Chongqing Medical UniversityChongqingP.R.China Department of Intensive Care Unit the Second Affiliated Hospital of Chongqing Medical UniversityChongqingP.R.China Department of Radiology the Second Affiliated Hospital of Chongqing Medical UniversityChongqingP.R.China Department of General Practice Chongqing General HospitalChongqingP.R.China Department of Diagnostic Radiology Yong Loo Lin School of MedicineNational University of SingaporeSingaporeSingapore Nanomedicine Translational Research Program NUS Center for NanomedicineYong Loo Lin School of MedicineNational University of SingaporeSingaporeSingapore

The blockade of cytoprotective autophagy has been demonstrated to effectively enhance the efﬁcacy of sonodynamic therapy(SDT).However,the limited recogni-tion of antiautophagy agents for autophagosomes impedes the clinical application of autophagy *** efﬁciently deliver hydroxychloroquine(HCQ),an autophagy inhibitor,to autophagosomes,we utilized a strategy based on in situ click chemistry between sulfhydryl(-SH)and maleimide(Mal)groups to trigger autophagosomes tracking and suppress tumor growth synergistically.A cascade nanoreactor was synthesized by encapsulating Mal-modiﬁed HCQ(MHCQ)into a manganese porphyrin-based metal-organic framework with sonosensitizer proper-ties,followed by poly(ethylene glycol)ylated liposomal membrane *** ultrasound irradiation,SDT-induced apoptotic cells released damaged proteins with free-SH groups,which MHCQ rapidly captured in situ via a Mal-thiol click *** autophagosomes actively wrapped damaged proteins for detoxiﬁ-cation,they simultaneously internalized HCQ anchored on *** this scenario,antiautophagy drugs could actively track intracellular autophagosomes instead of undergoing passive diffusion in the *** interaction between HCQ and autophagic vesicles was greatly enhanced,which strengthened the blocking efﬁ-ciency of autophagy and resulted in complete cell ***,this study with smart design provides a promising strategy for improving intracellular targeted delivery to autophagosomes,thereby enhancing antitumor therapy.

关键词： autophagosomes tracking autophagy blockade click reaction sonodynamic therapy synergy

铁皮石斛多糖对2型糖尿病小鼠降糖降脂的作用

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食品科学 2020年第21期41卷 127-132页

作者：王云威王景雪山西大学生命科学学院山西太原030006

目的:研究铁皮石斛多糖(Dendrobium officinale polysaccharide,DOP)对2型糖尿病小鼠降糖降脂作用,为铁皮石斛的充分利用提供药理学依据。方法:用四氧嘧啶对小鼠进行腹腔注射(100 mg/kg mb),构建小鼠2型糖尿病模型。将供试小鼠分为6组:... 详细信息

目的:研究铁皮石斛多糖(Dendrobium officinale polysaccharide,DOP)对2型糖尿病小鼠降糖降脂作用,为铁皮石斛的充分利用提供药理学依据。方法:用四氧嘧啶对小鼠进行腹腔注射(100 mg/kg mb),构建小鼠2型糖尿病模型。将供试小鼠分为6组:DOP高剂量组(450 mg/kg mb)、DOP中剂量组(300 mg/kg m_b)、DOP低剂量组(150 mg/kg mb)、正常对照组、阳性对照组(盐酸二甲双胍250 mg/kg mb)、模型组,连续灌胃4周。对供试小鼠进行血液生化水平(甘油三酯和总胆固醇)、肾功能指标(血清肌酐和尿素氮)以及抗氧化能力检测。结果:1)与模型组小鼠相比,铁皮石斛多糖对2型糖尿病小鼠具有明显的降糖效果,其DOP高剂量组降糖效果极显著(P<0.01)。与模型组小鼠相比,铁皮石斛多糖能够显著提高2型糖尿病小鼠糖耐量(P<0.05),高剂量DOP的作用效果与盐酸二甲双胍基本一致。2)与模型组小鼠相比,DOP能够显著降低2型糖尿病小鼠的总胆固醇、低密度脂蛋白胆固醇、甘油三酯浓度(P<0.05),显著增加高密度脂蛋白胆固醇浓度(P<0.05),表明DOP能够改善2型糖尿病小鼠的血脂代谢紊乱。3)与模型组小鼠相比,DOP能够显著降低2型糖尿病小鼠血清尿素氮和肌酐浓度(P<0.05),表明DOP能够改善2型糖尿病小鼠的肾脏损伤。4)与模型组小鼠相比,DOP能够显著提高2型糖尿病小鼠胰岛素水平、降低其胰岛素抵抗性。5)与模型组小鼠相比,DOP能够显著提高2型糖尿病小鼠的总抗氧化能力、超氧化物歧化酶活力、过氧化氢酶活力(P<0.05)。结论:铁皮石斛多糖能够显著提高糖尿病小鼠肝脏和胰腺的抗氧化能力,修复肝脏和胰腺氧化损伤,从而提高胰岛素含量和缓解胰岛素抵抗,因此具有降血糖的作用。铁皮石斛多糖也有改善糖尿病小鼠血脂代谢紊乱、改善肾脏损伤的作用。

关键词：铁皮石斛多糖降糖降脂作用脂质代谢 2型糖尿病

Cloning and Expression Analysis of THWRKY12 Gene from Tamarix hispida

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Agricultural Biotechnology 2013年第5期2卷 55-57页

作者： jingxue wang Yuekun TANG Qi LIU Chunqiang LIANG Yucheng wang State Key Laboratory of Forest Genetics and Tree Breeding Northeast Forestry University

[ Objective] This study aimed to clone and analyze the expression of THWRKY12 gene in Tamarix hispida. [Method] T. hispida seedlings were treated with 400 mmol/L NaC1 solutlon, 20% PEG and 100 μmol/L ABA, respectively. The expression of THIVRKY12 gene in different tissues was investigated by RT-PCR technology. [Result] Under treatments of NaC1 and PEG, the expression of THWRKY12 gene in different tissues of T. h/sp/da seedlings showed an overall upregulated trend, suggesting that THWRKYI2 is related with the saline-alkali resistance and drought resistance of T. hispida. Under ABA treatment, THWRKY12 gene had approximately the same expression pattern with the former two, indicating that THWRKY12 gene may involve in the regulation and control of the saline-al- kali resistance and drought resistance of T. hispida through the signal pathway regulated by ABA. [ Conclusion] This study laid foundation for investigating the functions of WRKY gene in the stress resistance of T. hispida.

关键词： Tamarix hispida WRKY transcription factor RT-PCR Saline-alkali stress Drought stress

Some properties of Young measure solutions for a class of nonlinear diffusion equations

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Progress in Natural Science:Materials International 2002年第7期 34-39页

作者： wang Chunpeng and YIN jingxue(Department of Mathematics, Jilin University, Changchun 130012, China)

This paper is concerned with the first initial-boundary value problem of a class of singular diffusion equations with the flux sublinear growth and the potential without convexity, in which the properties of Young measure solutions are discussed, and the uniqueness, stability are proved, and the asymptotic behaviors of solutions and their energy as the time goes to infinity are investigated.

关键词： singular diffusion equations forward-backward Young measure solutions.

Existence of Young measure solutions of a class of singular diffusion equations

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Progress in Natural Science:Materials International 2002年第4期 20-25页

作者： YIN jingxue and wang Chunpeng(Department of Mathematics, Jilin University, Changchun 130012, China)

The first initial-boundary value problem of a class of singular diffusion equations with the flux sublinear growth and the potential without convexity is investigated. Such equations may be strongly degenerate, singular and forward-backward. Inspired by the idea in a recent work of Demoulini, we first discuss the regular case by introducing the Young measure solutions and prove the existence of such solutions. Consequently, we approximate the extreme case by the method of regularization. By means of some uniform estimates and some techniques, the existence of Young measure solutions with bounded variation is established.

关键词： singular diffusion equations forward-backward Young measure solutions.