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Characterization of the Purified Glutathione S-transferases from Two Psocids Liposcelis bostrychophila and ***

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Agricultural Sciences in China 2010年第7期9卷 1008-1016页

作者： DOU Wei XIAO Li-sha NIU jin-zhi JIANG Hong-bo wang jin-jun Key Laboratory of Entomology and Pest Control Engineering College of Plant Protection Southwest University Chongqing 400716 P.R. China

Glutathione S-transferases（GSTs） from Liposcelis bostrychophila Badonnel and ***（Enderlein）（Psocoptera：Liposcelididae） were purified by glutathione-agarose affinity chromatography,and characterized subsequently by their Michaelis-Menten kinetics toward the artificial substrates 1-chloro-2,4-dinitrobenzene（CDNB） and reduced glutathione（GSH）,*** specific activity of the purified GST toward CDNB was 2.3-fold higher in *** than in *** the specific activities of purified enzymes varied between the two species,the purification yields were ***-PAGE revealed one band at 23 kDa for both the *** of *** exhibited higher Michaelis-Menten constants（Km） but lower maximal velocity（Vmax） values than those of *** optimum pH for CDNB conjugation of *** and *** GSTs was 7.0 and 7.5,and optimum temperature was 35 and 40°C,*** kinetics showed that cibacron blue,curcumin,bromosulfalein,ethacrynic acid,and carbosulfan had excellent inhibitory effects on GSTs in both species,but the inhibitory effects of beta-cypermethrin,fenpropathrin,tetraethylthiuram disulfide,and diethyl maleate were not significant.

关键词： GSTs purification psocids xenobiotic compounds

Adsorption and Desorption of Methiopyrsulfuron in Soils

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Pedosphere 2011年第3期21卷 380-388页

作者： WU Chun-Xian wang jin-jun ZHANG Su-Zhi ZHANG Zhong-Ming College of Plant Protection Southwest University Chongqing 400716 China Institute for the Control of Agrochemicals of Sichuan Province Chengdu 610041 China Maize Research Institute Sichuan Agricultural University Ya'an 625014 China

Methiopyrsulfuron is a new low-rate sulfonylurea herbicide for weed control in wheat; however, there is a lack of published information on its behavior in soils. In this study, methiopyrsulfuron adsorption and desorption were measured in seven soils sampled from Heilongjiang, Shandong, Jiangxi, Sichuan, Anhui, and Chongqing provinces of China using a batch equilibrium method. The Freundlich equation was used to described its adsorption and desorption. Adsorption isotherms were nonlinear with the values of Kf-ads, the Freundlich empirical constant indicative of the adsorption capacity, ranging from 0.75 to 2.46, suggesting that little of this herbicide was adsorbed by any of the seven soils. Soil pH and organic matter content (OM) were the main factors influencing adsorption; adsorption was negatively correlated with pH and positively correlated with OM. Methiopyrsulfuron desorption was hysteretic on the soils with high OM content and low pH.

关键词： Freundlich equation herbicide hysteresis organic matter pH

T_(2)WI 3D-SPACE+RS-EPI DWI检查对直肠癌术前肿瘤淋巴结分期诊断准确率的影响

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中国CT和MRI杂志 2024年第6期22卷 142-144页

作者：王俊波王晋君丁建峰张崇杰焦玉泉运城市中心医院影像科山西运城044000

目的探究T_(2)WI 3D-SPACE联合RS-EPI DWI检查在直肠癌术前肿瘤淋巴分期诊断准确率的影响。方法收集2020年7月-2022年2月在本院确诊直肠癌并进行影像学检查的96名患者作为此次的研究对象,将所有的研究对象随机进行分组,对照组选择T_(2... 详细信息

目的探究T_(2)WI 3D-SPACE联合RS-EPI DWI检查在直肠癌术前肿瘤淋巴分期诊断准确率的影响。方法收集2020年7月-2022年2月在本院确诊直肠癌并进行影像学检查的96名患者作为此次的研究对象,将所有的研究对象随机进行分组,对照组选择T_(2)WI 3D-SPACE进行检查,研究组采用T_(2)WI3D-SPACE联合RS-EPI DWI进行检查,由盲选形式经检查结果呈现给2位资历较高的临床医师对直肠癌术前肿瘤淋巴分期的结果进行鉴别,分析两组肿瘤淋巴结分期阳性诊断的准确性的差异。结果研究组在直肠癌术前是肿瘤淋巴结分期的准确率高于对照组,尤其是在T3期和T4期,对照组的准确率分别为75.61%和84.38%,研究组为97.56%和100%,有差异纳入统计(P<0.05);对照组的总准确率和淋巴结阳性准确率分别为70%和79.55%,研究组的总淮确率和淋巴结阳性准确率分别为95%和97.73%,研究组总准确率和淋巴结阳性准确率高于对照组,有差异纳入统计(P<0.05)。结论 T_(2)WI 3D-SPACE联合RS-EPI DWI检查能提高直肠癌术前肿瘤淋巴分期诊断的准确性,具有较高的诊断价值,可用于直肠癌术前的肿瘤淋巴结的扫描,为临床医生的确诊提供了重要的理论依据。

关键词： T_(2)WI 3D-SPACE RS-EPI DWI 直肠癌肿瘤淋巴分期诊断准确率

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Influence of Organic Amendments on Adsorption, Desorption and Leaching of Methiopyrisulfuron in Soils

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Journal of Integrative Agriculture 2013年第9期12卷 1589-1597页

作者： WU Chun-xian NIE Guo ZHANG Zhong-ming wang Guang-cheng GAO Li-ming wang jin-jun College of Plant Protection Southwest University Institute of the Control of Agrochemical of Sichuan Province

Methiopyrisulfuron is a novel sulfonylurea herbicide with good activity for annual broadleaf and gramineal weeds control. Present study was to investigate the effects of organic amendments （including peat （PE）, sewage sludge （SS）, and humic acid （HA）） on adsorption, desorption and leaching of methiopyrisulfuron in soils. The batch equilibration technique was applied for adsorption-desorption experiments and the leaching was tested through soil column simulated experiments under laboratory conditions. The Freundlich model may well describe adsorption-desorption of methiopyrisulfuron on organic amendments, the natural soil, and amended soils. Organic amendments could not only greatly increase the adsorption capacity of methiopyrisulfuron, but also significantly enhance the hysteresis of desorption of methiopyrisulfuron. The correlations between Kf_ads and organic matter content of amended soils were significant, and the correlations between H and soil organic matter in amended soils with PE, SS, and HA were significant too. The results of soil column experiments indicated that organic amendments greatly decreased leaching of methiopyrisulfuron. This study suggested that PE, SS, and HA could greatly influence environmental behavior of methiopyrisulfuron in soils. Use of organic amendments might be an effective management practice for controlling potential pollution of methiopyrisulfuron to environment.

关键词： organic amendment adsorption desorption leaching methiopyrisulfuron soil

Special Issue: Functional Polymer Materials

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Chinese Journal of Polymer Science 2024年第7期42卷 895-896,I0004页

作者： jun-Wei Gu Du-jin wang Shaanxi Key Laboratory of Macromolecular Science and Technology School of Chemistry and Chemical Engineering Northwestern Polytechnical University Xi’an 710072 China Beijing National Laboratory for Molecular Sciences CAS Key Laboratory of Engineering Plastics Institute of Chemistry Chinese Academy of Sciences Beijing 100190 China

The rapid development of science has led to a significant role for polymer materials in multitude fields,including industry,medicine,energy,and the environment,*** a leading academic journal in the field of polymer,Chinese Journal of Polymer Science has been dedicated to fostering the advancement and utilization of polymer science.

关键词： polymer Polymer Functional

RNA interference in insects:the link between antiviral defense and pest control

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Insect Science 2024年第1期31卷 2-12页

作者： jinzhi Niu Ruoyu Chen jin-jun wang Key Laboratory of Entomology and Pest Control Engineering College of Plant ProtectionSouthwest UniversityChongqingChina Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River(Ministry of Education) Southwest UniversityChongqingChina

RNA interference(RNAi)is a form of gene silencing triggered by double-stranded RNA(dsRNA)that operates in all eukaryotic *** has been widely investigated in insects to determine the underlying molecular mechanism,to investigate its role in systemic antiviral defense,and to develop strategies for pest *** insect cells are infected by viruses,viral dsRNA signatures trigger a local RNAi response to block viral replication and generate virus-derived DNA that confers systemic ***-based insect pest control involves the application of exogenous dsRNA targeting genes essential for insect development or survival,but the efficacy of this approach has limited potency in many pests through a combination of rapid dsRNA degradation,inefficient dsRNA uptake/processing,and ineffective RNAi *** could be addressed by dsRNA screening and evaluation,focusing on dsRNA design and off-target management,as well as dsRNA production and *** review summarizes recent progress to determine the role of RNAi in antiviral defense and as a pest control strategy in insects,addressing gaps between our fundamental understanding of the RNAi mechanism and the exploitation of RNAi-based pest control strategies.

关键词： RNAi response systemic RNAi insecticidal dsRNA biological control off-targets

Adsorption-Desorption Characteristics of Chlorimuron-Ethyl in Soils

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Agricultural Sciences in China 2007年第11期6卷 1359-1368页

作者： ZHANG Wei wang jin-jun ZHANG Zhong-ming QIN Zhen College of Plant Protection Southwest University Chongqing 400716 P.R.China Institute of the Control of Agrochemical of Sichuan Province Chengdu 610041 P.R.China

The adsorption-desorption characteristics of chlorimuron-ethyl in soils were investigated to provide the basic data for evaluating the safety in field and the risk to water resource. The adsorption-desorption experiment was conducted by the batch equilibration and HPLC techniques; furthermore, data were analyzed with 5 mathematic models to describe the characteristics and mechanism of adsorption-desorption and translocation of the herbicide in soils. The results showed that the adsorption-desorption isotherms of chlorimuron-ethyl fitted for the Freundlich model well, and the physical reaction presents the main contribution during the adsorption-desorption process. The adsorption values （Kads-f） of chlorimuron-ethyl in 8 types of soil ranged from 0.798 to 6.891. The isotherms of 2# （Jiangxi clay） and 3# （Jiangxi sand loam） soils belong to the S-type curve, while the isotherms of another 6 type soils belong to the L-type isotherm. The results of desorption indicated that the hysteresis phenomena appeared during the desorption process, and the hysteresis coefficients （H） of the herbicides in 8 soils varied from 0.259-0.980. Furthermore, Kads-f and desorption values （Kads-f） increased with the OM （%） and the clay content increasing, while the values decreased with the soils pH increasing. The H values decreased with the OM and the clay content increasing, and increased with the soils pH increasing. It can be concluded that the low adsorption abilities of chlorimuron-ethyl in test soils and un-reversible adsorption existed in the process, which will induce the great translocation of the herbicide after application in field. It can be transported to ground or groundwater causing risk to environments. The physical and chemical properties of soils, including the OM, the clay content, and the pH of soil were the dominating factors during the adsorption-desorption.

关键词： chlorimuron-ethyl adsorption-desorption Freundlich model hysteresis physical and chemical properties of soils

Identification,Characterization, and Expression of P450 Gene Encoding CYP6BQ13v2 from the Red Flour Beetle,Tribolium castaneum (Herbst) (Coleoptera:Tenebrionidae)

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Agricultural Sciences in China 2009年第10期8卷 1210-1218页

作者： XU Yong-qiang wang jin-jun JIANG Hong-bo DOU Wei TANG Pei-an AN Feng-ming Key Laboratory of Entomology and Pest Control Engineering College of Plant Protection Southwest University Chongqing 400716P.R. China

An allele of CYP6BQI3, named CYP6BQ 13v2 （GenBank accession no. FJ209361）, was isolated from the red flour beetle, Tribolium castaneum （Herbst）（Coleoptera： Tenebrionidae） by RT-PCR. The cDNA sequence of CYP6BQ13v2, 1 563 bp in length, contains an open reading frame of 1 554 nucleotides encoding a putative protein of 518 amino acid residues with a predicted molecular weight of 59.92 kDa and a theoretical pl of 7.60. The putative protein contains the classic hemebinding sequence motif F××G×××C×G （residues 456-465） conserved among all P450 enzymes as well as other characteristic motifs of all cytochrome P450s. It shares 98% identity with the previously published sequence of CYP6BQ13 （GenBank accession no. XP967146） from the T. castaneum genome project. Phylogenetic analysis of amino acid sequences from members of various P450 families indicated that there was closer phylogenetic relationship of CYP6BQ 13v2 with CYP302A1 and CYP307A1 mediating synthesis of the insect molting hormone, distant relationship with CYP6B1 metabolizing plant allelochemicals, CYP6D 1 linking to pyrethroid resistance and other members of CYP6 family. Expression test of the gene in the adults and immature stages of T. castaneum by quantitative real-time PCR revealed that CYP6BQ13v2 is expressed in all life stages investigated. The mRNA expression level in 1st instar larvae was 14.9- and 3.86-fold higher than those in pupae and adults, respectively. The CYP6BQ13v2 expression levels appeared in the order of 1st instar larvae, followed by 4th instar larvae, 7th instar larvae, adult, and pupae from high to low. The more bioinformation of CYP6BQ 13v2 was also analyzed.

关键词： Tribolium castaneum cytochrome P450 real-time PCR analysis bioinformatics

转录因子E74在柑桔全爪螨后若螨发育中的作用

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应用昆虫学报 2024年第3期61卷 539-548页

作者：刘钰杭李传振夏梦豪刘巽燕李玉闯潘登王进军豆威西南大学植物保护学院昆虫学及害虫控制工程重庆市重点实验室重庆400715 西南大学含弘学院重庆400715 西南大学农业科学研究院重庆400715

【目的】作为20E诱导产生的一个关键转录因子,E74在昆虫变态发育过程中起重要调控作用,但该转录因子在螨类中的研究较少。本文旨在探究转录因子E74在柑桔全爪螨Panonychus citri后若螨到成螨阶段的功能,为害螨提供潜在的防控靶点。【方... 详细信息

【目的】作为20E诱导产生的一个关键转录因子,E74在昆虫变态发育过程中起重要调控作用,但该转录因子在螨类中的研究较少。本文旨在探究转录因子E74在柑桔全爪螨Panonychus citri后若螨到成螨阶段的功能,为害螨提供潜在的防控靶点。【方法】利用RT-qPCR,明确了E74(PcE74)在柑桔全爪螨后若螨到成螨阶段的表达模式。进一步利用RNA干扰(RNA interference,RNAi)技术,通过人工装置饲喂法,以3种不同的处理(dsRNA,siRNA和dsRNA+siRNA)沉默PcE74,分析沉默效率以确定最佳处理方式,并探究了PcE74在柑桔全爪螨后若螨发育为成螨过程中的作用。【结果】PcE74在柑桔全爪螨后若螨到成螨各阶段均有表达,后若螨2 h至后若螨16 h表达量上升,后若螨16 h至发育为3日龄成螨表达量递减,成螨阶段表达量相对较低。RNAi表明,与单独饲喂ds PcE74(14%)或si PcE74(24%)相比,饲喂ds PcE74+si PcE74干扰效率显著提高(54%)(P<0.05),且成螨羽化率显著下降(P<0.05),异常表型表现为静伏期的后若螨不能产生旧表皮而死亡。dsRNA+siRNA的RNAi效率优于单独使用dsRNA或siRNA。【结论】PcE74参与调控柑桔全爪螨后若螨到成螨的发育过程,与E74互作的基因调控机制有待进一步研究。

关键词：柑桔全爪螨 PcE74 RNAi siRNA 羽化率