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Diagnosis of West Nile virus infections:Evaluation of different laboratory methods

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World Journal of Virology 2024年第4期13卷 51-61页

作者： Tatjana Vilibic-Cavlek.Maja Bogdanic vladimir Savic Zeljk. Hrusk.r Ljubo Barbic vladimir Stevanovic Ljiljana Antolasic Ljiljana Milasincic Dario Sabadi Gorana Miletic Ivona Coric Anna Mrzljak.Eddy Listes Giovanni Savini Department of Virology Croatian Institute of Public HealthZagreb 10000Croatia School of Medicine University of ZagrebZagreb 10000Croatia Poultry Center Croatian Veterinary InstituteZagreb 10000Croatia Department of Microbiology and Infectious Diseases with Clinic Faculty of Veterinary Medicine University of ZagrebZagreb 10000Croatia Department of Infectious Diseases Clinical Hospital Center OsijekOsijek 31000Croatia Medical Faculty Josip Juraj Strossmayer University of OsijekOsijek 31000Croatia Department of Gastroenterology and Hepatology University Hospital Center ZagrebZagreb 10000Croatia Croatian Veterinary Institute Veterinary Institute SplitSplit 21000Croatia OIE Reference Center for West Nile Disease Istituto Zooprofilattico SperimentaleG.CaporaleTeramo 64100Italy

BACk.ROUND The diagnosis of West Nile virus(WNV)is challenging due to short-term and low-level viremia,flavivirus cross-reactivity,and long immunoglobulin M(IgM)*** To evaluate different methods for WNV detection[reverse transcription-polymerase chain reaction(RT-PCR),IgM/IgG antibodies,IgG avidity]in serum,cerebrospinal fluid(CSF),and urine samples of patients with confirmed WNV *** The study included patients with confirmed WNV neuroinvasive infection(n=62),asymptomatic WNV seropositive individuals(n=22),and individuals with false-positive WNV IgM antibodies(n=30).WNV RNA was detected using RT-PCR.A commercial ELISA was used to detect WNV IgM/IgG antibodies with confirmation of cross-reactive samples using a virus neutralization test(VNT).IgG-positive samples were tested for IgG *** The WNV-RNA detection rates were significantly higher in the urine(54.5%)/serum(46.4%)than in CSF(32.2%).According to the sampling time,the WNV-RNA detection rates in urine collected within 7 days/8-14/≥15 days were 29.4/66.6/62.5%(P=0.042).However,these differences were not observed in the *** median RT-PCR cycle threshold values were significantly lower in urine(32.5,IQR=28-34)than in CSF(34.5,IQR=33-36).The frequency of positive WNV IgM and IgG significantly differed according to the sampling time in serum but not in *** IgM/IgG antibodies were detected in 84.3/9.3%of serum samples collected within 7 days,100/71.1%of samples collected 8-14,and 100%samples collected after≥15 *** WNV infection was confirmed by low/borderline avidity index(AI)in 13.6%of asymptomatic individuals.A correlation between ELISA and AI was strong negative for IgM and strong positive for *** significant correlation between ELISA IgG and VNT was *** The frequency of WNV RNA and antibody detection depends on the sampling time and type of clinical *** avidity could differentiate recent WNV infections from long-persisting IgM antibo

关键词： West Nile virus Reverse transcription-polymerase chain reaction Serology IgG avidity Cross-reactivity

Evaluating the Efficacy of Cervical Tactile Ultrasound Technique as a Predictive Tool for Spontaneous Preterm Birth

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Open Journal of Obstetrics and Gynecology 2024年第5期14卷 832-846页

作者： vladimir Egorov Todd Rosen Jennifer Hill Meena k.andelwal Victors k.rtenok. Brendan Francy Noune Sarvazyan Advanced Tactile Imaging Inc. Ewing New Jersey USA Department of Obstetrics Gynecology and Reproductive Sciences Rutgers Robert Wood Johnson Medical School New Brunswick New Jersey USA Department of Maternal-Fetal Medicine Cooper Medical School of Rowan University Camden New Jersey USA

Back.round: Premature cervical softening and shortening may be considered an early mechanical failure that predispose to preterm birth. Purpose: This study aims to explore the applicability of an innovative cervical tactile ultrasound approach for predicting spontaneous preterm birth (sPTB). Materials and Methods: Eligible participants were women with low-risk.singleton pregnancies in their second trimester, enrolled in this prospective observational study. A Cervix Monitor (CM) device was designed with a vaginal probe comprising four tactile sensors and a single ultrasound transducer operating at 5 MHz. The probe enabled the application of controllable pressure to the external cervical surface, facilitating the acquisition of stress-strain data from both anterior and posterior cervical sectors. Gestational age at delivery was recorded and compared against cervical elasticity. Results: CM examination data were analyzed for 127 women at 240/7 - 286/7 gestational week.. sPTB was observed in 6.3% of the cases. The preterm group exhibited a lower average cervical stress-to-strain ratio (elasticity) of 0.70 ± 0.26 k.a/mm compared to the term group’s 1.63 ± 0.65 k.a/mm with a p-value of 1.1 × 10−4. Diagnostic accuracy for predicting spontaneous preterm birth based solely on cervical elasticity data was found to be 95.0% (95% CI, 88.5 - 100.0). Conclusion: These findings suggest that measuring cervical elasticity with the designed tactile ultrasound probe has the potential to predict spontaneous preterm birth in a cost-effective manner.

关键词： Cervical Elasticity Spontaneous Preterm Birth Tactile Ultrasound Probe

A rare case of cardiac and ophthalmic manifestations of meningococcal septicemia

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World Journal of Emergency Medicine 2024年第4期15卷 328-330页

作者： Svetlana Rachina Anastasia Shchendrygina Heshan Radeesha de Silva Svetlana Tarasenk. Nadezhda Muk.ina Irina k.marova Ludmila Fedina Elena Burmistrova Elvira Martens Sergey Sidorenk. vladimir Gostev Dmitry Polev Alina Saitova Lyudmila k.aeva Nik.ta Goncharov Irina Zvetk.va Department of Hospital Therapy I M Sechenov First Moscow State Medical UniversityMoscow 119048Russia Outpatient Clinic#64 Moscow 119048Russia Department of Cardiology City Clinical Hospital n.a.S.S.YudinMoscow 119048Russia Department of Medical Microbiology and Molecular Epidemiology Pediatric Research and Clinical Center for InfectiousDiseasesSaint Petersburg 197022Russia Department of Medical Microbiology North-Western State Medical University Named After I.I.MechnikovSaintPetersburg 191015Russia Department of Epidemiology Saint-Petersburg Pasteur InstituteSaint Petersburg 197101Russia

Acute pericarditis is usually a self-limiting disorder that commonly occurs due to viral etiology or unexplained *** pericarditis(MP)is a remark.bly uncommon clinical entity that manifests as a complication during the acute or convalescent phase of meningococcal ***,its occurrence within the context of meningococcal septicemia has also been *** report a rare case of a previously healthy male patient who developed both acute pericarditis and uveitis as a presentation of meningococcal septicemia,with no characteristic sk.n rashes or meningeal signs.

关键词： acute Acute rare

Multiple-Photon Resonance Enabled Quantum Interference in Emission Spectroscopy of N2+

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Ultrafast Science 2024年第2期4卷 1-7页

作者： Xiang Zhang Qi Lu YaLei Zhu Jing Zhao Rostyslav Danylo Liang Xu Mingwei Lei Hongbing Jiang Chengyin Wu Zhedong Zhang Aurélien Houard vladimir Tik.onchuk.AndréMysyrowicz Qihuang Gong Songlin Zhuang Zengxiu Zhao Yi Liu Shanghai k.y Lab of Modern Optical System University of Shanghai for Science and Technology.516Jungong Road200093 ShanghaiChina Department of Physics National University of Defense TechnologyChangsha 410073China Laboratoire d'Optique Appliquee ENSTA ParisEcole Polytechnique CNRSInstitut Polytechnique de Paris828 Boulevard des Marechaux91762 Palaiseau CedexFrance State k.y Laboratory for Mesoscopic Physics School of PhysicsPeking UniversityBejing 100871China Department of Physics City University of Hong KongKowloonHong Kong SAR Centre Lasers Intenses et Applications University of Bordeaux-CNRS-CEA351 Cours de la Liberation33405 Talence Cedex France Extreme Light Infrastructure ERIC ELI Beamlines Facility25241 Doln BrezanyCzech Republic CAS Center for Excellence in Ultra-intense Laser Science Shanghai 201800China

Quantum interference occurs frequently in the interaction of laser radiation with materials,leading to a series of fascinating effects such as lasing without inversion,electromagnetically induced transparency,Fano resonance,*** quantum interference effects are mostly enabled by single-photon resonance with transitions in the matter,regardless of how many optical frequencies are ***,we report on quantum interference driven by multiple photons in the emission spectroscopy of nitrogen ions that are resonantly pumped by ultrafast infrared laser *** the spectral domain,Fano resonance is observed in the emission spectrum,where a laser-assisted dynamic Stark.effect creates the *** the time domain,the fast-evolving emission is measured,revealing the nature of free-induction decay arising from quantum radiation and molecular *** findings clarify the mechanism of coherent emission of nitrogen ions pumped with mid-infrared pump laser and are found to be *** present work.opens a route to explore the important role of quantum interference during the interaction of intense laser pulses with materials near multiple photon resonance.

关键词： pumped pump Quantum

Study of Sands from Bamendou-West Cameroon: Valorization for Concrete Mix Design

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Journal of Geoscience and Environment Protection 2024年第5期12卷 345-354页

作者： k.ubou Tatapzia vladimir Willianov Guimezap k.nou Willy Chance Manefouet Bertile Ilalie k.mga Djoumen Tatiana Ngapgue François Faculty of Sciences University of Dschang Dschang Cameroon Institut universitaire de la Cô te Douala Cameroun Department of Civil Engineering FOTSO Victor University Institute of Technology University of Dschang Bandjoun Cameroon

Bamendou (West Cameroon), has a huge potential in natural resources, especially sands. However, the use of these materials in civil engineering work. leads to the appearance of numerous pathologies which in some cases lead to the total ruin of the work.. In order to overcome these infrastructural problems, the main objective of this study is set at the improvement of the service life of structures built in Cameroon using local materials formed under climatic, geological and geotechnical conditions similar to those of materials in Bamendou. Eight sand samples were tak.n from the most representative and exploited quarries. The identification and classification of the sand tak.n from the most representative quarries in the study area show that they are mainly clayey, with an average sand equivalent of 57.54. In terms of granulometry, the curves of several sand samples do not fall within the granular range of sands used in the formulation of concrete. The modulus of fineness obtained by particle size analysis varies from 2.91 to 3.92 with an average of 3.31.

关键词： Residual Sand Formulation of Concrete Sand Equivalent Fitness Modulus Particle Size Bamendou

Hetero-integrated high-peak.optical-power laser source (940 nm) for time-of-flight sensors

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Chinese Optics Letters 2024年第7期22卷 81-85页

作者： Sergey Olegovich Slipchenk. Alek.andr Alek.androvich Podosk.n Ilia Vasil'evich Shushk.nov Marina Gennad'evna Rastegaeva Artem Eduardovich Rizaev Matvey Igorevich k.ndratov Artem Evgen'evich Grishin Nik.ta Alek.androvich Pik.tin Timur Anatol'evich Bagaev Maxim Anatol'evich Ladugin Alek.andr Anatol'evich Marmalyuk.vladimir Alek.androvich Simak.v Ioffe Institut Russian Academy of SciencesSt.Petersburg 194021Russia Open Joint-Stock.Company M.F.Stel’mak. Polyus Research Institute Moscow 117342Russia RUDN University Moscow 117198Russia

The generation of high-power laser pulses using a compact hetero-integrated assembly based on a semiconductor laser with a dual-element composite 2μm×100μm aperture and a compact heterothyristor switch is *** achieved peak.optical power was 33 W with a pulse duration of 3 ns at a thyristor operating voltage of 55 *** leading edge of the laser pulse turn-on was 50 ps to a power level of 24.7 W,and the turn-on delay between the elements of the composite aperture was 160 ps.

关键词： hetero-integrated assembly semiconductor lasers thyristor switches LIDAR gain-switching mode laser heterostructure

Elucidating elusive quaternary selenide EuCeCuSe3:Synthesis,crystal structure,properties and theoretical studies

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Journal of Rare Earths 2024年第1期42卷 163-171,I0006页

作者： Maxim V.Grigoriev Anna V.Ruseik.na Maxim S.Molok.ev vladimir A.Chernyshev Alek.andr S.Alek.androvsk. Alexander S.k.ylov Svetlana N.k.ylova Nik.lai P.Shestak.v Dmitriy A.Velik.nov Alexander A.Garmonov Alexey V.Matigorov Evgeny A.Ostapchuk.Thomas Schleid Damir A.Safin Laboratory of Theory and Optimization of Chemical and Technological Processes University of TyumenVolodarskogo Str.6625003TyumenRussian Federation k.rensk. Institute of Physics Federal Research Center KSC SB RASAkademgorodok 50 Bld.38660036KrasnoyarskRussian Federation Department of Physics Far Eastern State Transport UniversitySerysheva Str.47680021KrasnoyarskRussian Federation Institute of Natural Sciences and Mathematics Ural Federal University Named After the First President of Russia B.N.YeltsinMira Str.19620002EkaterinburgRussian Federation Department of Photonics and Laser Technology Siberian Federal UniversitySvobodnii Ave.79660079KrasnoyarskRussian Federation Institute of Physics and Technology University of TyumenVolodarskogo Str.6625003 TyumenRussian Federation Institute for Inorganic Chemistry University of StuttgartPfaffenwaldring 55D-70569StuttgartGermany Scientific and Educational and Innovation Center for Chemical and Pharmaceutical Technologies Ural Federal University named after the First President of Russia B.N.YeltsinMira Str.19620002 EkaterinburgRussian Federation University of Tyumen Volodarskogo Str.6625003TyumenRussian Federation

We report on the novel heterometallic quaternary selenide EuCeCuSe3,the fabrication of which has been a challenge until this *** structure of the reported selenide was elucidated from the powder X-ray diffraction data,which revealed the formation of EuCeCuSe3with excellent yield(96.7%)accompanied with a minor fraction of CeSe2(3.3%),and was best solved in orthorhombic space group Pnma with the BaLaCuS3structural ***,the crystal structure of the title compound completes the row of the heterometallic quaternary selenides EuRECuSe3(RE=La,Pr,Nd,Sm,Eu,Gd,Tb,Dy,Ho,Er,Tm,Yb,Lu,Y),of which the cerium-based derivative exclusively belongs to the BaLaCuS3structural *** distortion of the CuSe4polyhedron was compared for the whole series of EuRECuSe3compounds using theτ4-descriptor for four coordinated ions,which revealed the highest degree of distortion for the Ce3+-containing selenide,followed by the La3+-based ***,the crystallographic and geometrical parameters of the reported selenide were discussed in comparison to the Ce3+-based sulfides SrCeCuS3and *** initio calculations of the crystal structure,a phonon spectrum and elastic constants for the crystal of EuCeCuSe3were also *** types and wavenumbers of fundame ntal modes were determined and the involvement of ions participating in the phonon modes was *** experimental IR spectrum of the reported selenide was interpreted and found to be in agreement with the calculated *** experimental direct band gap of EuCeCuSe3was measured to be 1.36 eV that is consistent with the concept of its origin due to interband transitions between orbitals emerging mainly from 4f(valence band)and 5d(conduction band)levels of the Eu2+*** dependence of the Young’s modulus on the direction demonstrates the anisotropy of the elastic properties,while the Vick.rs hardness for EuCeCuSe3was calculated to be 5.2 ***,the title compound is paramagnetic above 4 k.

关键词： Quaternary selenide Synthesis Crystal structure Ab initio calculations Magnetic measurements Spectroscopy

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中红外大模场单模全固态硫系光子晶体光纤

中国激光 2024年第17期 248-256页

作者：李宁哲贾金升张敏王煜泽肖峰黄庆白胜闯 vladimir Shiryaev 戴世勋聂秋华王训四宁波大学信息科学与工程学院高等技术研究院红外材料与器件实验室浙江省光电探测材料及器件重点实验室俄罗斯科学院高纯物质化学研究所宁波海洋研究院

针对当前硫系光纤难以同时实现高功率传输与高光束质量的问题，通过COMSOL软件仿真模拟光纤性质，设计出了满足单模传输需要且具有大模场面积的光子晶体光纤（PCF）的结构参数。所设计的光纤在1.5～10μm的波长范围内满足高阶模(LP11)... 详细信息

针对当前硫系光纤难以同时实现高功率传输与高光束质量的问题，通过COMSOL软件仿真模拟光纤性质，设计出了满足单模传输需要且具有大模场面积的光子晶体光纤（PCF）的结构参数。所设计的光纤在1.5～10μm的波长范围内满足高阶模(LP11)与基模(LP01)的限制损耗比大于10～2的单模条件，且光纤基模的有效模面积在波长2μm处约为5362μm2。根据仿真模拟的结构参数，探索出新型动、静态蒸馏相结合的技术，制备出高纯硫系玻璃，结合新型的挤压-堆积法，制备出基于全硫系玻璃的全固态大模场单模光子晶体光纤。该光纤在波长1.55μm处的近高斯光能量分布特征验证了其单模特性；同时在弯曲半径为14 cm的1.5 m长的光纤中观察到低弯曲损耗为1 dB，与仿真结果基本一致，表明此全固态光子晶体光纤具有出色的单模传输和抗弯性能，在中红外高能激光传输方面具备一定的应用潜能。

关键词：光纤光学中红外全固态光子晶体光纤硫系玻璃大模场面积单模传输

20%PEG胁迫下评价引进哈萨克斯坦不同春小麦种质苗期的抗旱性

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新疆农业科学 2024年第6期61卷 1352-1360页

作者：王一钊杨其志刘玉秀阿拉依·努尔卡马力 vladimir Shvidchenk. 张正茂西北农林科技大学农学院陕西杨凌712100 哈萨克斯坦赛福林农业技术大学阿斯塔纳010000

【目的】研究引进哈萨克斯坦不同春小麦种质的萌发和生理特性,为选育适宜我国西北旱区春小麦品种提供亲本材料。【方法】采用20%聚乙二醇(polyethylene glycol,PEG)高渗溶液模拟干旱胁迫条件,比较分析28份小麦种质(其中引进哈萨克斯坦... 详细信息

【目的】研究引进哈萨克斯坦不同春小麦种质的萌发和生理特性,为选育适宜我国西北旱区春小麦品种提供亲本材料。【方法】采用20%聚乙二醇(polyethylene glycol,PEG)高渗溶液模拟干旱胁迫条件,比较分析28份小麦种质(其中引进哈萨克斯坦春小麦种质25份)苗期的根长、苗长、根鲜/干重、苗鲜/干重、发芽率、发芽势及发芽指数的变化,利用隶属函数、主成分分析综合评价不同春小麦种质苗期抗旱性。【结果】与对照相比,干旱胁迫显著降低种质苗期的根长、苗长、根鲜重、苗鲜/干重、发芽势和发芽率(P<0.05),降低幅度为18.29%~52.17%;显著增加根干重(P<0.05),平均增加了66.67%。各性状间存在不同程度的相关性,大多数性状抗旱系数间存在显著或极显著相关。9个小麦苗期抗旱相关指标可转换为4个(苗鲜/干重、苗长和发芽率)独立的综合指标可作为哈萨克斯坦春小麦苗期耐旱性评价的主效指标。28份供试材料的加权抗旱指数进行聚类分析,5份为抗旱性强种质,17份为抗旱性种质,6份为抗旱性弱种质。【结论】25份引进哈萨克斯坦春小麦种质中有5份种质,苗期具有较强的萌发特性和抗旱能力,适宜在我国西北旱区春小麦育种中提供抗旱亲本材料。

关键词：春小麦引种种质苗期抗旱性隶属函数