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Isolation and Characterization of Novel Microsatellite Markers in Chayote[Sechium edule(Jacq.)Sw.]

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American Journal of Plant Sciences 2015年第13期6卷 2033-2041页

作者： Ryoko Machida-Hirano Moisés Cortés-Cruz Blanca Amalia Amaro González Jorge cadenaí Iiguez Kazuto Shirata Kazuo N.Watanabe Gene Research Center University of TsukubaTsukubaJapan Centro Nacional de Recursos Genéticos Instituto Nacional de Investigaciones ForestalesAgrícolas y PecuariasTepatitlán de MorelosMéxico Colegio de Postgraduados Campus San Luis PotosíSalinas de HidalgoMexico National Institute of Agrobiological Sciences TsukubaJapan

Chayote [Sechium edule (Jacq.) Sw.] is an economically important species in Latin America;however, there are very few reports available regarding its genetic diversity. Out of 11 microsatellite markers isolated, 10 loci provided 1 to 7 alleles per locus in a set of Mexican chayote accessions. Observed and expected heterozygosities for each locus ranged from 0.00 to 0.85 and 0.00 to 0.73, respectively. The overall genetic diversity detected by microsatellites was compared with that detected by P450-based analogue markers, a genome-wide dominant marker. Genetic diversity values obtained by the newly designed microsatellite markers were almost equal to the value estimated by PBA markers, but genetic distances calculated by both marker systems were not significantly correlated. Additional microsatellite markers, which could detect more polymorphisms, may be necessary to analyze the genetic diversity and structure of Mexican chayote collections.

关键词： Chayote SSR Markers PBA Markers Dual-Suppression-PCR Technique Genetic Diversity

Identification of life habits factors as risk for gastritis and colitis occurrence in a mestizo population of Chabeklumil, Chiapas, México

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Open Journal of Nursing 2012年第2期2卷 67-71页

作者： Diana Cecilia Tapia-Pancardo Ramiro Jesús-Sandoval Myrna Miriam Valera-Mota José Luis cadena-Anguiano Miguel Murguía-Romero Rafael Villalobos-Molina Carrera de Cirujano Dentista FES Iztacala Universidad Nacional Autónoma de México Tlalnepantla México Carrera de Médico Cirujano FES Iztacala Universidad Nacional Autónoma de México Tlalnepantla México Carrera de Optometría FES Iztacala Universidad Nacional Autónoma de México Tlalnepantla México División de Investigación y Posgrado FES Iztacala Universidad Nacional Autónoma de México Tlalnepantla México

Aim: Gastric diseases are a recent trend in morbidity in México. We aimed to identify most frequent life habits factors as risk for gastritis and colitis occurrence, in a Mexican mestizo population in Chabeklumil, Chiapas. Population: This study was observational, descriptive, transversal and prospective;346 questionnaires were applied to individuals of both genders from 11 years of age, with low income and socio-economic status, after they signed the informed consent. An individual file was created by the nurses in Chabeklumil. The predominant gender in the population was female (56.1%), with a distribution of 166 among adulthood (19 - 59 years of age);among males (43.9%), 133 included adults. Results: We identified the following environmental risk factors to develop gastritis and colitis: long periods of daily fasting > consumption of irritant food and drink > stress, in females;in contrast, males reported alcohol and tobacco consumption > irritant food and drink > long periods of daily fasting > stress. We found statistical differences (P < 0.05) in risk factors between females and males, in drugs and alcohol consumption, fasting, and stress, but not in consumption of irritant foods, nor in colitis and gastritis prevalence. Conclusions: Females are most affected by life habits risk factors for gastritis/colitis, which occurs mainly due to lifestyle and “macho” culture, since women are precluded of a better living standard, they work hard in the field, and start early in the day without consuming a meal, which they eat late in the day once arriving home. People in Chabeklumil eat/drink a lot of irritant food like hot pepper and coffee, which damage the gastrointestinal system leading to gastritis and colitis. Furthermore, men started to consume drugs at early ages, a condition also involved in developing gastritis.

关键词： Gastritis Colitis Life Habits Risk Factors Poverty Mexican Mestizo Population

Entire Whitening of Apple Trees to Increase Chill Units in Regions of Warm Temperatures during the Fall-Winter Seasons

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American Journal of Plant Sciences 2022年第8期13卷 1100-1115页

作者： Alejandro Zermeño-González J. A. Gil-Marín H. Ramírez-Rodríguez A. I. Melendres-Alvarez M. cadena-Zapata Departamento de Riego y Drenaje Universidad Autonóma Agraria Antonio Narro Saltillo México Departamento de Horticultura Universidad Autonóma Agraria Antonio Narro Saltillo México Departamento de Maquinaria Agrícola Universidad Autonóma Agraria Antonio Narro Saltillo México

Different studies have shown that global warming and climate change have increased the planet’s temperature in different locations. For the apple-growing farmers, this may have a negative impact on the accumulations of chill units when the air temperature during the fall-winter season increases. When the entire trees are covered with a reflective material, the wood temperature may decrease. Therefore, the objective of this study was to evaluate the effect of whitening (with calcium hydroxide) the entire apple trees (Malus domestica Borkh) after defoliation, on the branches and trunks’ internal temperature (under the bark), the accumulation of chill units (CU), its effects on fruit yield and quality and the relation with the use of thidiazuron (TDZ) (inducer of budbreak). The study was conducted during the fall-winter seasons of 2019-2020 and 2020-2021. The results of this study showed that at the hours of the highest incidence of solar radiation, the internal temperature of the whitened trunks and leaves decreases up to 9°C and 6°C respectively. The accumulated CU during the time of the study, of the whitened branches were up to 81% higher than the ones recorded on the branches with no whitening;while the CU was lost at the hours of highest solar radiation (due to a high temperature) were up to 37.2% smaller. Entire tree whitening increased up to 26% of the yield per tree compared to the application of TDZ. No statistical difference in fruit quality was observed between whitened trees and those with no whitening but with the application of thidiazuron.

关键词： Malus domestica Borkh Chill Units Whitening Calcium Hydroxide Budbreak

PANDAS PARTICIPAN EN HOCKEY Y CANAN

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China & The World Cultural Exchange 2022年第2期88卷 F0004-F0004页

作者： Jose Eduardo cadena Ortiz 不详

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Masa餐厅

现代装饰 2019年第3期 36-43页

作者： Benjamin cadena(摄影) Naho Kubota(摄影) Ambiente Familiar(摄影) 彭佳(译) 纪少佳纽约Studio cadena设计事务所不详

项目地点哥伦比亚波哥大项目面积697m2美编纪少佳图室内空间采用了水磨石地板以及带有纹理的现浇混凝土墙壁纽约Studio cadena设计事务所近日在哥伦比亚波哥大北部住宅区设计了一座新的Masa餐厅。该项目原本是一座住宅,多年来经历了多... 详细信息

项目地点哥伦比亚波哥大项目面积697m2美编纪少佳图室内空间采用了水磨石地板以及带有纹理的现浇混凝土墙壁纽约Studio cadena设计事务所近日在哥伦比亚波哥大北部住宅区设计了一座新的Masa餐厅。该项目原本是一座住宅,多年来经历了多次住宅和商业的更迭。餐厅内部空间共约697平方米,包括多个独立且相连的体块,每个体块都有各自特定的功能。空间的一角是咖啡厅兼面包店,随之即是中心入口区,紧邻餐区和一个独立的零售空间。

关键词：室内空间

Problem-Based Learning <i>in Situ</i>Applied to Students in the Assessment of Nursing Process

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Open Journal of Nursing 2021年第5期11卷 378-389页

作者： Margarita Acevedo-Peñ a Rosa María Ostiguín-Meléndez José Luis cadena-Anguiano Marcela Patricia Ibarra-Gonzalez Jesús Rigoberto Hernández-Hernández Rafael Villalobos-Molina Diana Cecilia Tapia-Pancardo Facultad de Estudios Superiores Iztacala Universidad Nacional Autónoma de México Mexico City Mexico Escuela Nacional de Enfermería y Obstetricia Universidad Nacional Autónoma de México Mexico City Mexico Facultad de Estudios Superiores Iztacala Universidad Nacional Autónoma de México Mexico City Mexico Colegio de Posgrado de la Ciudad de México city Mexico Unidad de Biomedicina Facultad de Estudios Superiores Iztacala Universidad Nacional Autónoma de México Mexico City Mexico

Background: Nursing is considered a science in the making that recognizes the nursing process (NP) as the scientific and methodological tool to provide care. The NP is composed of five stages of which the assessment is the first and fundamental to identify the health problems of the people. This research aimed to evaluate a pedagogical didactic strategy that is based on situated constructivism, the Based-Problem Learning (BPL), in order to improve significant learning. Methods: Quantitative research with an explanatory scope, was transversal, and it was applied to 30 students of the first year of the nursing career at the Faculty of Higher Studies Iztacala, National Autonomous University of Mexico. The sample was chosen considering the inclusion criteria. Data were collected using a Likert-type instrument validated in a previous investigation. Results: The average value obtained from the Likert scale to evaluate the intervention was 103.4, the highest score was 135 and the lowest was 83, which reflects that it was evaluated as good and particularly good. The grade given to the teacher as an agent of the ZPD, was between agree and totally agree, the highest percentages scored at 93%, since it provoked challenge in their thinking, and favored interaction among classmates. Conclusions: The application of the in situ teaching strategy, BPL favored significant learning of the assessment stage, the students were able to carry out the assessment in the hospital, the experience contributed to generate significant learning when students face real scenarios, which showed the effectiveness of the method.

关键词： Learning Method Nursing Process Assessment

Silver Recovery from Spent Silver Oxide Button Cell by Liquid-Liquid Extraction

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Journal of Materials Science and Chemical Engineering 2015年第7期3卷 148-153页

作者： Zeferino Gamino-Arroyo Antonio Tapia-Cisneros Osvaldo M.Zamacona-Saucedo Irene Cano-Rodríguez Alberto F.Aguilera-Alvarado Lorena E.Sánchez-cadena Fernando I.Gómez-Castro Departamento de Ingeniería Química Universidad de Guanajuato Guanajuato México Departamento de Ingeniería Civil Universidad de Guanajuato Guanajuato México

Button cell batteries are used in clocks, thermometers, remote controls, toys and other devices, and they are usually discarded in the trash once its useful life is over. Some models of these batteries contain silver oxide. In this paper we propose liquid-liquid extraction as separation process to recover the metal. First, silver determination is performed in different models of these batteries and leaching with nitric acid is carried out. Affinity study is done between several commercial extractants for silver. The best performing extractant is the bis(2-ethylhexyl) dithiophosphoric acid (D2EHDTPA). Furthermore, a study of the extraction yields as a function of extractant concentration and time is performed. The distribution isotherm is determined;complex extracted in organic phase and stripping conditions have been identified. With the aim of obtaining industrial application, a number of steps for a countercurrent process were defined by the McCabe-Thiele method. Finally, a study was done in micropilot scale. The results show that it is possible to recover silver from this type of waste.

关键词： Silver Spent Button Cells Leaching Recovery

Growth of Four Varieties of Coffea arabica L. Biofertilized with Rhizophagus intraradices and Azospirillum brasilense in Nursery

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The Beneficial Effect of Trichoderma spp. in Seed Treatment of Four Maize (Zea mays L.) Genotypes

American Journal of Plant Sciences（美国植物学期刊） 2023年第5期

作者： Zoila C. Anzueto-Herón Juan F. Aguirre-Medina Juan F. Aguirre-cadena cadena-Iñiguez Pedro

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American Journal of Plant Sciences 2023年第6期14卷 625-637页

作者： José Luis Arispe-Vázquez Abiel Sánchez-Arizpe Daniel Alejandro cadena-Zamudio Ma. Elizabeth Galindo-Cepeda David Heriberto Noriega-Cantú Oscar Guadalupe Barrón-Bravo Leslie Carnero-Avilés Juan Mayo-Hernández Susana Elizabeth Ramírez-Sánchez Adriana Antonio-Bautista Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias Iguala de la Independencia Guerrero México Departamento de Parasitología Universidad Autónoma Agraria Antonio Narro Saltillo México Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias Altamira México Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias Delicias México Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias Tepatitlán de Morelos Jalisco México Departamento de Fitomejoramiento Universidad Autónoma Agraria Antonio Narro Saltillo México

Maize is the main crop for Mexicans;however, it is affected by species of fungi causing ear rot. This research aimed to evaluate the effect of T. asperellum T11, T. harzianum T1 4 y T. longibrachiatum T1 40 on some agronomic variables of four maize genotypes. The seeds of the genotypes H-515, Zapata 7, and H-507 were treated with a suspension of Trichoderma spp. to 1 × 108 spores mL-1, using a control (untreated seed), and Benomyl as chemical control. The planting was in Morelos, in a completely random block design with a factorial arrangement. The ear rot was natural. Data were obtained at the end of the crop cycle and processed in SAS 9.4®. H-515 genotype had the greatest effect on the treatment of maize seeds with Trichoderma spp. (5.562 kg);T. asperellum T11 was the strain that stood out with a mean yield of 50 ears in an area of 16 m2 of 4.904 kg, and control of 4.448 kg. Our results are an economic option for farmers to contemplate the use of Trichoderma and obtain its benefits.

关键词： Seeds Treatment Trichoderma asperellum Ear Rot Yield