Isolation and Characterization of Novel Microsatellite Markers in Chayote[Sechium edule(Jacq.)Sw.]
隔离和新型微卫星标记佛手瓜的表征佛手瓜属edule（黄灯笼）西南。]作者机构：Gene Research CenterUniversity of TsukubaTsukubaJapan Centro Nacional de Recursos GenéticosInstituto Nacional de Investigaciones ForestalesAgrícolas y PecuariasTepatitlán de MorelosMéxico Colegio de PostgraduadosCampus San Luis PotosíSalinas de HidalgoMexico National Institute of Agrobiological SciencesTsukubaJapan
出 版 物：《American Journal of Plant Sciences》 (美国植物学期刊（英文）)
年 卷 期：2015年第6卷第13期
页 面：2033-2041页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：This research was supported by JST/JICA SATREPS(Science and Technology Research Partnership for Sustainable Development)“Diversity Assessment and Development of Sustainable Use of Mexican Genetic Resources:a SATREPS Project” Japan INIFAP and Interdisciplinary Research Group Sechium edule in Mexico(GiSeM)
主 题：Chayote SSR Markers PBA Markers Dual-Suppression-PCR Technique Genetic Diversity
摘 要：Chayote [Sechium edule (Jacq.) Sw.] is an economically important species in Latin America;however, there are very few reports available regarding its genetic diversity. Out of 11 microsatellite markers isolated, 10 loci provided 1 to 7 alleles per locus in a set of Mexican chayote accessions. Observed and expected heterozygosities for each locus ranged from 0.00 to 0.85 and 0.00 to 0.73, respectively. The overall genetic diversity detected by microsatellites was compared with that detected by P450-based analogue markers, a genome-wide dominant marker. Genetic diversity values obtained by the newly designed microsatellite markers were almost equal to the value estimated by PBA markers, but genetic distances calculated by both marker systems were not significantly correlated. Additional microsatellite markers, which could detect more polymorphisms, may be necessary to analyze the genetic diversity and structure of Mexican chayote collections.
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