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Four new phenolic glycosides from Baoyuan decoction

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Acta Pharmaceutica Sinica B 2017年第2期7卷 173-178页

作者： Xiaoli Ma Xiaoyu Guo Mingbo Zhao pengfei tu Yong Jiang State Key Laboratory of Natural and Biomimetic Drugs School of Pharmaceutical SciencesPeking UniversityBeijing 100191China

Four new phenolic glycosides,including two flavonoid glycosides(1 and 2) and two lignan glycosides(3 and 4),were isolated from the traditional Chinese medicine formula,Baoyuan *** structures were established by detailed analysis of the NMR and HR-ESI-MS spectroscopic data and their absolute configurations were determined by the experimental electronic circular dichroism data as well as chemical ***,the sources of the four new compounds were determined by the UPLC-Qtrap-MS method,which proved that 1 and 2 are originated from Glycyrrhiza uralensis,and 3 and4 are from Cinnamomum cassia.

关键词： Baoyuan decoction Traditional Chinese med icine formula Flavonoid glycosides Lignan glycosides Electronic circular dichroism UPLC–Qtrap-MS

Effects of parasitic plant Cistanche deserticola on chlorophyll a fluorescence and nutrient accumulation of host plant Haloxylon ammodendron in the Taklimakan Desert

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Journal of Arid Land 2012年第3期4卷 342-348页

作者： Li LI XinWen XU YongQiang SUN WeiHAN pengfei tu Cele National Station of Observation & Research for Desert-Grassland Ecosystem in Xinjiang Cele 848300 China Xinjiang Institute of Ecology and Geography Chinese Academy of Sciences Urumqi 830011 China State Key Laboratory of Natural and Biomimetic Drugs School of Pharmaceutical Sciences Peking University Health ScienceCenter Beijing 100191 China

The parasitic plant Cistanche deserticola attaches to Haloxylon ammodendron, a perennial shrub with high tolerance to salinity and drought. However, little was known about the parasite-host relation between the two species. Effects of the parasite on chlorophyll a fluorescence and nutrient accumulation in the host plant （H. am- modendron） were investigated in the Taklimakan Desert. Some photosynthetic parameters of both host and non-host H. ammodendron plants were measured by in vivo chlorophyll a fluorescence technology in the field. The assimilating branches of host and non-host plants were collected and nutrient and inorganic ion contents were analyzed. The results from field experiments showed that the infection of C. deserticola reduced the non-photochemical quenching of the variable chlorophyll fluorescence （NPQ） and the potential maximum quantum yield for primary photochemistry （Fv/Fm） of the host. Compared with non-host plants, the host H. ammodendron had low nutrient, low inorganic ion contents （Na~ and K~） and low K~/Na~ ratios in the assimilating branches. It suggested that C. deserticola infection reduced the nutrient acquisition and caused damage to the photoprotection through thermal dissipation of the energy of the photosystem II in the host, resulting in a decrease in the tolerance to salinity and high radiation. It was concluded that the attachment of the parasite plant （C. deserticola） had negative effects on the growth of its host.

关键词： parasite-host relation nutrient acquisition inorganic ion content non-photochemical quenching the Taklimakan Desert

Application of ~1H NMR-based metabolomics for discrimination of different parts and development of a new processing workflow for Cistanche deserticola

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Acta Pharmaceutica Sinica B 2017年第6期7卷 647-656,676页

作者： Pingping Zou Yuelin Song Wei Lei Jun Li pengfei tu Yong Jiang State Key Laboratory of Natural and Biomimetic Drugs School of Pharmaceutical Sciences Peking University Modern Research Center for Traditional Chinese Medicine School of Chinese Materia Medica Beijing University of Chinese Medicine

Cistanche deserticola(CD) is one of the two authoritative source plants of Cistanches Herba, a well-known medicinal plant. Herein,~1H NMR spectroscopy was employed to characterize the chemical profile and to distinguish the different parts, as well as to propose a new processing workflow for *** assignment was achieved by multiple one and two dimensional NMR spectroscopic techniques in combination with available databases and authentic compounds. The upper parts of the plant were distinguished from the lower parts by combining ~1H NMR spectroscopic dataset with multivariate statistical analysis. A new processing method that hyphenated steaming with freeze-drying, was demonstrated to be superior to either steaming coupled with oven-drying or direct freeze-drying via holistic ~1H NMR-based metabolomic characterization. Phenylethanoid glycosides, mainly echinacoside and acteoside, were screened out and confirmed as the chemical markers responsible for exhibiting the superiority of the new processing workflow, whereas serial primary metabolites, especially carbohydrates and tricarboxylic acid cycle metabolites, were found as the primary molecules governing the discrimination between the upper and lower parts of the plant. Collectively,~1H NMR spectroscopy was demonstrated as a versatile analytical tool to characterize the chemical profile and to guide the indepth exploitation of CD by providing comprehensive qualitative and quantitative information.

关键词： Cistanche deserticola 1H NMR-based metabolomics Processing workflow Different parts Phenylethanoid glycoside Tricarboxylic acid cycle metabolites Echinacoside Acteoside

Cytotoxic polyacetylenes isolated from the roots and rhizomes of Notopterygium incisum

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Chinese Chemical Letters 2019年第2期30卷 428-430页

作者： Xikang Zheng Xiaoqing Zheng Chen Zhang Qingying Zhang Yong Jiang pengfei tu State Key Laboratory of Natural and Biomimetic Drugs School of Pharmaceutical Sciences Peking University

Phytochemical investigation on the roots and rhizomes of Notopterygium incisum led to the isolation of a new polyacetylene, notopolyenol A (1), along with thirteen known analogues (2-14). Their structures were elucidated by extensive analyses of NMR and HRMS data, and the absolute configuration of 1 was unambiguously determined as 3 R by comparison of its retention time and ECD curve with those of synthetic enantiomers (-)-1 and (+)-1, whose absolute configurations were established by using the modified Mosher's method. Subsequent activity screening revealed that (3 S)-1 exhibited the most significant cytotoxicity against MCF-7, H1299, and HepG2 cancer cells with IC_(50) values of 1.3 μmol/L,0.6μmol/L and 1.4μmol/L, respectively.

关键词： Notopterygium incisum Polyacetylenes Total synthesis Cytotoxicity

NO inhibitory constituents from Glycosmis craibii ***

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Chinese Journal of Natural Medicines 2024年第11期22卷 1040-1046页

作者： CHEN Hongwei DING Meng LIN Jun YUAN Shuo ZENG Kewu tu pengfei JIANG Yong State Key Laboratory of Natural and Biomimetic Drugs School of Pharmaceutical SciencesPeking UniversityBeijing 100191China

Six novel compounds,comprising three quinolones(1a,1b,and 2)and three flavanones(3-5),along with seven known analogs(6-13),were isolated from the 95%EtOH extract of the stems and leaves of Glycosmis craibii *** structures of the new compounds were elucidated using HR-ESI-MS,UV,and 1D and 2D nuclear magnetic resonance(NMR)data *** ab-solute configurations were determined through Mosher ester and electronic circular dichroism(ECD)spectral *** 2,6,9,and 10 demonstrated inhibition of nitric oxide(NO)production stimulated by lipopolysaccharide in BV-2 microglial cells,with IC50 values ranging from 13.5 to 20.1 μmol·L^(-1),comparable to the positive control,dexamethasone.

关键词： Glycosmis craibii var.glabra Quinolone Flavanone NO inhibition

Anti-psoriasis molecular targets and active components discovery of Optimized Yinxieling Formula via affinity-purified strategy

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Chinese Journal of Natural Medicines 2024年第2期22卷 127-136页

作者： WANG Wei LIU Lijuan YANG Zhuo LU Chuanjian tu pengfei ZHAO Ruizhi ZENG Kewu State Key Laboratory of Natural and Biomimetic Drugs School of Pharmaceutical SciencesPeking UniversityBeijing 100191China State Key Laboratory of Dampness Syndrome of Chinese Medicine The Second Affiliated Hospital of Guangzhou University of Chinese MedicineGuangzhou 510006China

Psoriasis,a prevalent inherited skin condition,involves an inflammatory response as a key pathogenic *** Optimized Yinxieling Formula(OYF),rooted in traditional Chinese medicine,is extensively utilized in clinical settings to treat *** previous studies have demonstrated OYF’s significant anti-inflammatory effects in psoriasis,its potential molecular targets and active components remain *** study aimed to unveil the anti-psoriasis molecular targets and active components of *** findings indicated that OYF extract markedly reduced the production of several inflammatory mediators,including IL-23,nitric oxide,TNF-α,and IL-1β,in LPS-induced RAW264.7 *** synthesized OYF extract-crosslinked beads to isolate pharmacological targets from RAW264.7 lysates using an affinity purification strategy,known as Target *** enriched target proteins were subsequently identified via LC-MS/MS,followed by bioinformatics analysis to map the psoriasis-associated pathway-gene *** identified a total of 76 potential target proteins,which were highly associated with mRNA transcription *** particular,pathway-gene network analysis revealed that the IL-23 inflammatory pathway was involved in the anti-psoriasis effect of OYF *** further utilized a target protein-based affinity capture strategy,combined with LC-MS and SPR analysis,to globally screen OYF’s active components,focusing on the mRNA transcription regulator,fused in sarcoma(FUS).This process led to the identification of umbelliferone,vanillic acid,protocatechuic acid,gentisic acid,and echinacoside as key compounds targeting FUS to inhibit IL-23 ***,we formulated a compound cocktail(CpdC),which significantly reduced psoriasis area and severity index(PASI)scores and the expressions of IL-23 and Ki67 in an imiquimod(IMQ)-induced psoriasis mouse ***,our study elucidates the primary molecular targets and active components of OYF,offeri

关键词： Psoriasis Optimized Yinxieling Formula(OYF) FUS protein Molecular targets Affinity-purified strategy

Rapid Simultaneous Determination of Four Ganoderic Acids in Ganoderma(Chinese Name:Lingzhi)by Direct Infusion-Multiple Reaction Monitoring Cubed

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Journal of Analysis and Testing 2024年第1期8卷 52-62页

作者： Nian Wu Bo Peng Ting Li pengfei tu Shengpeng Wang Bo Li Wenjing Liu Yuelin Song Modern Research Center for Traditional Chinese Medicine Beijing Research Institute of Chinese MedicineBeijing University of Chinese MedicineBeijing 100029China Amway(China)Botanical Research Center Wuxi 214011China State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical SciencesUniversity of MacaoTaipa 999078China Amway(Shanghai)Innovation&Science Co. LtdShanghai 201508China School of Pharmacy Henan University of Chinese MedicineZhengzhou 450046China

Attributing to the rapid demand expansion for the edible medicinal materials in the market,the limited throughput of highperformance liquid chromatography-multiple reaction monitoring(HPLC-MRM)cannot fully address the measurement workload for a huge number of testing ***,it is urgent to pursue more efficient approaches for the quality *** of the greater selectivity of MRM cubed(MRM^(3))over MRM,there might be a chance to omit the time-intensive LC *** current study,we attempted to develop a direct infusion(DI)-MRM^(3) program,and the applicability was thereafter assessed through simultaneous determination of four ganoderic acids(GAs)in one of the most famous tonic herbal medicines namely Ganoderma(Chinese name:Lingzhi).Primary parameters such as Q1>Q3>QLIT ion transitions,collision energy(CE),and excitation energy were optimized by programming online energy-resolved mass spectrometry with authentic compounds.A single DI-MRM measurement merely costed four minutes,and in spite of the wide occurrences of isomers,satisfactory selectivity was *** validation assays demonstrated the method to be sensitive,precise,accurate,and *** quantitative results from DI-MRM^(3) were also justified by conducting LC-MRM measurements in *** differences occurred for the content patterns between the two original sources namely Ganoderma lucidum and ***,and,moreover,either cultivar or harvest time showed dramatical influence on the quantitative features of the four targeted *** importantly,DI-MRM3 is a meaningful analytical option for rapid quantitative analysis of herbal medicines,because of the comparable reliability,nonetheless,less consumptions of both measurement time and solvent,compared with LC-MRM.

关键词： Direct infusion Multiple reaction monitoring cubed High-throughput quantitative analysis Ganoderic acids Ganoderma

Triple three-dimensional MS/MS spectrum facilitates quantitative ginsenosides-targeted sub-metabolome characterization in notoginseng

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Acta Pharmaceutica Sinica B 2024年第9期14卷 4045-4058页

作者： Ke Zhang Jinru Jia Ting Li Wenjing Liu pengfei tu Jian-Bo Wan Jun Li Yuelin Song Modern Research Center for Traditional Chinese Medicine Beijing Research Institute of Chinese MedicineBeijing University of Chinese MedicineBeijing 100029China SCIEX China Beijing 100015China School of Pharmacy Henan University of Chinese MedicineZhengzhou 450046China State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical SciencesUniversity of MacaoTaip1Macao 999078China

Although serving as the workhorse,MS/MS cannot fully satisfy the analytical requirements of quantitative sub-metabolome *** more information intrinsically correlates to more structural and concentration clues,here,efforts were devoted to comprehensively tracing and deciphering MS/MS behaviors through constructing triple three-dimensional(3×3D)-MS/MS ***-targeted metabolomics of notoginseng,one of the most famous edible medicinal plants,was employed as a *** authentic ginsenosides were deployed to build the correlations between 3×3D-MS/MS spectra and structure/concentration *** assaying ginsenosides with progressive concentrations using QTOF-MS to configure 1st 3D spectrum,the generations of MS1 spectral signals,particularly multi-charged multimer anions,e.g.,[2Me2H]^(2-) and[2M+2HCOO]^(2-) ions,relied on both concentration and the amount of sugar *** programming progressive collision energies to the front collision cell of Qtrap-MS device to gain 2^(nd) 3D spectrum,optimal collision energy(OCE)corresponding to the glycosidic bond fission was primarily correlated with the masses of precursor and fragment ions and partially governed by the glycosidation *** quantitative relationships between OCEs and masses of precursor and fragment ions were utilized to build large-scale quantitative program for *** applying progressive exciting energies to the back collision chamber to build 3^(rd) 3D spectrum,the fragment ion and the decomposition product anion exhibited identical dissociation trajectories when they shared the same molecular *** ginsenosides-focused quantitative metabolomics,significant differences occurred for sub-metabolome amongst different parts of *** differential ginsenosides were confirmatively identified by applying the correlations between 3×3D-MS/MS spectra and ***,3×3D-MS/MS spectrum covers all MS/MS behaviors and drama

关键词： Quantitative submetabolome characterization Ginsenosides Structural identification Notoginseng Full exciting energy ramp-MS3 spectrum

Impact of Motor Stator Winding Faults on Motor Differential-mode Impedance and Mode Transformation

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Chinese Journal of Electrical Engineering 2022年第3期8卷 12-21页

作者： Fei Fan Zhenyu Zhao Huamin Jie Quqin Sun pengfei tu Zhou Shu Wensong Wang Kye Yak See School of Electrical and Electronic Engineering Nanyang Technological UniversitySingapore 639798Singapore Science and Technology on Thermal Energy and Power Laboratory Wuhan Second Ship Design and Research InstituteWuhan 430000China

Motor impedance and mode transformation have significant effects on the electromagnetic interference(EMI)generated in motor drive *** winding faults commonly cause motor failure;however,in their early stages,they may not affect the short-term operation of the *** date,EMI noise under the influence of premature stator winding faults has not been adequately studied,particularly the differential-mode(DM)noise due to the common-mode(CM)-to-DM *** study investigates and quantifies the influence of stator winding faults on the motor DM impedance and mode ***,the transmission line model of an induction motor is described based on the scattering(S)parameter measurements of each phase of the *** offers the flexibility to emulate different types of stator winding faults at specific locations and various severities,such that the impacts of the faults on the motor DM impedance can be easily ***,a test setup is proposed to quantify the CM-to-DM transformation due to the stator winding *** findings of this study reveal that even the early stages of stator winding faults can result in significant changes in the DM noise.

关键词： Differential-mode(DM)impedance DM noise mode transformation motor stator winding faults transmission line model