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World Journal of Vaccines 2014年第4期4卷 164-174页

作者： toshiaki shimoyama Takumi Kubota Junsuke Shirai Rie Watanabe Laboratory of Epizootiology Cooperative Department of Veterinary Medicine Faculty of Agriculture Tokyo University of Agriculture and Technology Fuchu Japan Research and Education Center for Prevention of Global Infectious Disease of Animals Fuchu Japan Veterinary Hygiene Joint Faculty of Veterinary Medicine Yamaguchi University Yamaguchi Japan

Four capsid proteins (VP1, VP2, VP3, and VP4) of coxsackievirus B3 (CVB3) were expressed as recombinant proteins in an Escherichia coli expression system and used as antigens for subunit vaccines against CVB3 in ICR mice. Antigens were expressed as thioredoxin-histidine (TrxHis)-tagged protein and purified before immunization. Although all VPs other than VP4 induced anti-CVB3 specific antibodies in mice (detected by ELISA and western blotting), they did not neutralize the infectious CVB3 in a virus neutralization assay. Meanwhile, 2 virus strains were purified from CVB3 virus stock on the basis of their plaque size on HeLa cells. ICR mice were infected with the 2 purified virus strains (S-strain and L-strain) and unpurified virus stock (wild type) to analyze the difference in antibody responses against infections of purified and unpurified virus strains. The reactivity of antisera against each virus strain was tested by ELISA, and the results showed that the inoculation of purified virus strain induced a strong antibody response against the inoculated strain. As a result, the antibody response against wild-type and other virus strains was suppressed. These results suggest using unpurified virus stock as an antigen is advantageous for inducing a broad antibody response in inoculated animals.

关键词： Coxsackievirus B3 Antibody Response Cross Reactivity

Enhanced Disinfection Effect of a Compound Disinfectant against Bovine Herpes Virus Type 1 at Low Temperature

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Open Journal of Veterinary Medicine 2013年第5期3卷 247-251页

作者： Makoto Nagai Ryoji Kamimura Reiji Seki toshiaki shimoyama Takumi Kubota Junsuke Shirai Department of Veterinary Medicine Faculty and Agriculture Tokyo University of Agriculture and Technology Tokyo Japan

A compound disinfectant, which consisted of didecyldimethylammonium chloride (DDAC), ortho-dichlorobenzene (1,2-dichlorobenzene, ODB), and chlorocresol (4-chloro-3-methylphenol, CC), and its component chemicals were individually tested for effectiveness against bovine herpes virus type 1 (BHV-1). DDAC, DDAC+ODB, DDAC+CC, and DDAC+ODB+CC showed effectiveness against BHV-1 at room temperature. However, ODB, CC, and ODB+CC showed no virucidal effects. The effects of all disinfectants tested were decreased at low temperature. DDAC showed disinfectant effects at a dilution of 1/800 and DDAC+ODB, DDAC+CC, and DDAC+ODB+CC at dilutions of 1/800 and 1/1600 at low temperature in the presence of 2% fetal bovine serum (FBS) but ODB, CC, and ODB+CC showed no virucidal effects. At low temperature and in the presence of 10% FBS, DDAC, DDAC+ODB, and DDAC+CC showed disinfectant effects at dilutions of 1/800, whereas ODB, CC, and ODB+CC showed no virucidal effects. DDAC+ ODB+CC was more effective (at 1/800 and 1/1600) than the other disinfectants under these conditions. In conclusion, a combination of three disinfectant components (DDAC+ODB+CC), enhanced the disinfectant effects at low temperature and in organic matter contamination.

关键词： Bovine Herpes Virus Type 1 Compound Disinfectant Enhancing Disinfection Low Temperature Organic Matter Contamination

Diabetes mellitus carries a risk of gastric cancer:A metaanalysis

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World Journal of Gastroenterology 2013年第40期19卷 6902-6910页

作者： Shouji shimoyama Gastrointestinal Unit Settlement ClinicTokyo 120-0003Japan

AIM:To investigate the association and quantify the relationship between diabetes mellitus(DM) and gastric cancer(GC) by an updated ***:The initial PubMed search identified 1233publications. Studies not reporting GC or those not reporting actual number of GC were excluded. Twelve pertinent studies were retrieved from the PubMed database or from a manual search and considered for the meta-analysis. Pooled risk ratios and 95%CI were estimated by a random-effects model. Subgroup analysis was performed according to gender or geographical regions. Heterogeneity and publication bias were evaluated by I2and funnel plot analysis,***:DM was significantly associated with GC with a RR of 1.41(P = 0.006)(95%CI:1.10-1.81).Subgroup analyses revealed that both sexes showed a significant association with GC,with a greater magnitude of risk in females(RR = 1.90; 95%CI:1.27-2.85;P = 0.002) than in males(RR = 1.24; 95%CI:1.08-1.43;P = 0.002). In addition,the link between DM and GC was significant in East Asian DM patients(RR = 1.77;95%CI:1.38-2.26; P < 0.00001) but not in Western DM patients(RR = 1.23; 95%CI:0.90-1.68; P = 0.2).There was no evidence of publication bias,but the results indicated significant ***:This updated meta-analysis has provided evidence of positive DM-GC associations. The limited information on potentially important clinical confounding factors in each study deserves further investigation.

关键词： Gastric cancer Diabetes mellitus Metaanalysis Hyperglycemia Hyperinsulinemia

Stool antigen tests for the management of Helicobacter pylori infection

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World Journal of Gastroenterology 2013年第45期19卷 8188-8191页

作者： Tadashi shimoyama Department of Gastroenterology Hirosaki University Graduate School of Medicine Hirosaki 036-8562Japan

Stool antigen tests(SATs)are noninvasive diagnostic modules for Helicobacter pylori(***)*** types of SATs exist for the diagnosis of *** infection,one based on enzyme immunoassay(EIA)and another on immunochromatography(ICA).SATs do not require expensive chemical agents or specified equipment;hence,they are less expensive compared with the urea breath *** European and Japanese guidelines have shown that EIA-based SATs using monoclonal antibodies are useful for primary diagnosis as well as for the assessment of eradication ***-based tests do not require particular equipment and are therefore useful in developing *** are also useful for the diagnosis of *** infection in children and post gastric surgery *** performed via EIA can assess *** infection in a large number of subjects,almost as well as ***,SATs would be useful or detecting current infection in such a survey to identify and eradicate *** *** accuracy of SATs is lower when the stool samples are unformed or watery,because *** antigens in the stool samples are *** and the interval between stool sample collection and measurement also affect the results of *** choice of test kit depends on the sensitivity and specificity in each region and the circumstances of each patient.

关键词： Helicobacter pylori Stool antigen test Diagnosis Enzyme immunoassay Immunochromatography

Assessment of physical stress during the perioperative period of endoscopic submucosal dissection

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World Journal of Gastroenterology 2022年第32期28卷 4508-4515页

作者： Daisuke Chinda Tadashi shimoyama Department of Gastroenterology Hirosaki University Graduate School of MedicineHirosaki 036-8562Japan Division of Endoscopy Hirosaki University HospitalHirosaki 036-8563Japan Department of Internal Medicine Aomori General Health Examination CenterAomori 030-0962Japan

The advantage of endoscopic submucosal dissection(ESD)is that it is less invasive than *** is one of the best treatments for older patients as surgery in this age group of patients is ***,it is unclear how much lower the physical stress of ESD is compared with that of ***,objective methods are required to assess physical stress in patients who have undergone *** current review of ESD aimed to summarize the recent advancements in the assessment of physical stress during the perioperative period,focusing on changes in energy metabolism and serum opsonic activity(SOA).Based on metabolic changes,resting energy expenditure(REE)was measured using an indirect *** stress factor calculated from the REE and the basal energy expenditure computed using the Harris-Benedict equation can be used to assess physical *** was assessed using the chemiluminescence method,wherein the use of chemiluminescent probes(i.e.,lucigenin and luminol)allowed quantification of reactive oxygen species generated by *** an auto luminescence analyzer,the results were evaluated based on the maximum light emission and area under the emission *** quantifiable results revealed the minimal invasiveness of ESD.

关键词： Physical stress Endoscopic submucosal dissection Indirect calorimeter Resting energy expenditure Chemiluminescence Serum opsonic activity

Impact of Helicobacter pylori infection on gut microbiota

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World Journal of Gastroenterology 2021年第37期27卷 6224-6230页

作者： Chikara Iino Tadashi shimoyama Department of Gastroenterology Hirosaki University Graduate School of MedicineHirosaki 036-8562Japan Department of Internal Medicine Aomori General Health Examination CenterAomori 030-0962Japan

A number of studies have revealed the association between Helicobacter pylori(***)infection and the gut *** than half of the investigations on the impact of *** on the gut microbiota have been the sub-analyses of the influence of eradication *** was observed that *** eradication altered gut microbiota within a short period after eradication,and majority of the alterations took a long period of time to reverse back to the *** in the gut microbiota within a short period after eradication may be attributed to antibiotics and proton pump *** of gastric acidity in the stomach caused by a long-term *** infection alters the gut *** of the gut microbiota should be conducted in a large population,adjusting for considerable biases associated with the composition of the gut microbiota,such as age,sex,body mass index,diet and the virulence of ***.

关键词： Helicobacter pylori Gut microbiota Atrophic gastritis Eradication Proton pump inhibitor

Controlling the aggregation and assembly of boron-containing molecular and polymeric materials

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Aggregate

Aggregate 2022年第2期3卷 50-59页

作者： Daisuke shimoyama Frieder Jäkle Department of Chemistry Rutgers University-NewarkNewarkNew JerseyUSA

Boron-containing molecules and polymers are attractive as powerful tunable Lewis acids for small molecule activation and catalysis,as luminescent materials for organic electronic device and(bio)imaging applications,and as smart chemical *** the characteristics of the boron-containing building blocks are attractive in and of themselves,their assembly into higher order supramolecular materials offers access to unique properties and emerging ***,we highlight recent achievements in the field of aggregated organoboron *** discuss how supramolecular interactions can be exploited to precisely control the structure of the assemblies and impact their functions as luminescent materials,recyclable and smart catalyst systems,chemical sensors,stimuli-responsive and self-healing materials.

关键词： boron luminescent materials nanostructure self-assembly supramolecular polymers

Trajectories of endoscopic Barrett esophagus: Chronological changes in a community-based cohort

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World Journal of Gastroenterology 2016年第35期22卷 8060-8066页

作者： Shouji shimoyama Toshihisa Ogawa Toshiyuki Toma Gastrointestinal Unit Settlement Clinic

AIM To elucidate longitudinal changes of an endoscopic Barrett esophagus(BE), especially of short segment endoscopic BE(SSBE). METHODS This study comprised 779 patients who underwent two or more endoscopies between January 2009 and December 2015. The intervals between the first and the last endoscopy were at least 6 mo. The diagnosis of endoscopic BE was based on the criteria proposed by the Japan Esophageal Society and was classified as long segment(LSBE) and SSBE, the latter being further divided into partial and circumferential types. The potential background factors that were deemed to affect BE change included age, gender, antacid therapy use, gastroesophageal reflux disease-suggested symptoms, esophagitis, and hiatus hernia. Time trends of a new appearance and complete regression were investigated by Kaplan-Meier curves. The factors that may affect appearance and complete regression were investigated by χ~2 and Student-t tests, and multivariable Cox regression analysis. RESULTS Incidences of SSBE and LSBE were respectively 21.7% and 0%, with a mean age of 68 years. Complete regression of SSBE was observed in 61.5% of initial SSBE patients, while 12.1% of initially disease free patients experienced an appearance of SSBE. Complete regressions and appearances of BE occurred constantly over time, accounting for 80% and 17% of 5-year cumulative rates. No LSBE development from SSBE was observed. A hiatus hernia was the only significant factor that facilitated BE development(P = 0.03) or hampered(P = 0.007) BE regression. CONCLUSION Both appearances and complete regressions of SSBE occurred over time. A hiatus hernia was the only significant factor affecting the BE story.

关键词： Barrett esophagus Longitudinal vessels Progression Regression Hiatus hernia

Kostka functions associated to complex reflection groups and a conjecture of Finkelberg-Ionov

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Science China Mathematics 2018年第2期61卷 353-384页

作者： toshiaki Shoji Department of Mathematics Tongji University

Kostka functions K_(λ,μ)~±(t), indexed by r-partitions λ and μ of n, are a generalization of Kostka polynomials K_(λ,μ)(t) indexed by partitions λ,μ of n. It is known that Kostka polynomials have an interpretation in terms of Lusztig's partition function. Finkelberg and Ionov(2016) defined alternate functions K_(λ,μ)(t) by using an analogue of Lusztig's partition function, and showed that K_(λ,μ)(t) ∈Z≥0[t] for generic μ by making use of a coherent realization. They conjectured that K_(λ,μ)(t) coincide with K_(λ,μ)^-(t). In this paper, we show that their conjecture holds. We also discuss the multi-variable version, namely, r-variable Kostka functions K_(λ,μ)~±(t_1,…,t_r).

关键词： Kostka functions complex reflection groups conjecture of Finkelberg-Ionov

Performance and Internal Flow of a Counter-rotating Type Tidal Stream Turbine

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Journal of Thermal Science 2015年第5期24卷 410-416页

作者： Bin Huang toshiaki Kanemoto Faculty of Engineering Kyushu Institute of Technology

In the past decade, the tidal energies have caused worldwide concern as it can provide regular and predictable re- newable energy resource for power generation. The majority of technologies for exploiting the tidal stream energy are based on the concept of the horizontal axis propellers, which can be derived from the design and operation of wind turbines. However, there are some peculiar features such as the propeller working in the seawater with free surface and the possible occurrence of cavitation as compared with wind turbines. Especially, for a coun- ter-rotating type tidal stream power turbine, it is difficult to accurately predict the interaction between the front and rear blades at the design stage by blade element momentum theory. As a result, CFD shows its advantage to predict the performance of counter-rotating type propellers of the tidal stream turbi^le. In order to improve the accuracy of CFD predictions, the predicted results must be verified with experimental values. In this paper, a CFD model using block-structured grid was set up and experimental test was performed in a water tunnel for a tidal stream turbine with counter-rotating type propellers. The comparison between CFD predictions and experimental data shows quite good agreement on the power coefficients, which provides an evidence of validation of the CFD model. Such results offer the necessary confidence in the accuracy of the set up CFD model for the coun- ter-rotating type tidal stream turbine.

关键词： Counter-rotating tidal stream turbine internal flow CFD experimental validation