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Increased osteogenesis with hydroxyapatite constructs combined with serially-passaged bone marrow-derived mesenchymal stem cells

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Stem Cell Discovery 2012年第4期2卷 133-140页

作者： Manabu Akahane tomoyuki ueha Takamasa Shimizu Yusuke Inagaki Akira Kido Tomoaki Imamura Kenji Kawate Yasuhito Tanaka Department of Artificial Joint and Regenerative Medicine Nara Medical University Nara Japan Department of Orthopedic Surgery Nara Medical University Nara Japan Department of Public Health Health Management and Policy Nara Medical University School of Medicine Nara Japan

We have previously reported on both the osteogenic potential of hydroxyapatite (HA) combined with bone marrow-derived mesenchymal stem cells (BMSCs) and a method involving osteogenic matrix cell sheet transplantation of BMSCs. In the present study, we assessed the osteogenic potential of serially-passaged BMSCs, both in vitro and in vivo. We also assessed whether an additional cell-loading technique can regain the osteogenic potential of the constructs combined with serially-passaged BMSCs. The present study revealed that passage (P) 1 cells cultured in osteogenic-induced medium showed strong positive staining for alkaline phosphatase (ALP) and Alizarin Red S, whereas P3 cells showed faint staining for ALP, with no Alizarin Red S staining. Staining of P1, P2 and P3 cells were progressively weaker, indicating that the osteogenic potential of the serially-passaged rat BMSCs is lost after P3 in vitro. The in vivo study showed that little bone formation was observed in the HA constructs seeded with P3 cells, 4 weeks after subcutaneous implantation. However, P3 cell/HA constructs which had increased cell-loading showed abundant bone formation within the pores of the HA construct. ALP and osteocalcin mRNA expression in these constructs was significantly higher than that of constructs with regular cell-seeding. The present study indicates that the osteogenic potential of the constructs with serially-passaged BMSCs is increased by additional cell-loading. This method can be applied to cases requiring hard tissue reconstruction, where BMSCs require serial expansion of cells.

关键词： Osteogenesis Mesenchymal Stem Cells Serial Passaging Hydroxyapatite Tissue Engineering Bone Reconstruction

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Utility of tricalcium phosphate and osteogenic matrix cell sheet constructs for bone defect reconstruction

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World Journal of Stem Cells 2015年第5期7卷 873-882页

作者： tomoyuki ueha Manabu Akahane Takamasa Shimizu Yoshinobu Uchihara Yusuke Morita Naoya Nitta Akira Kido Yusuke Inagaki Kenji Kawate Yasuhito Tanaka Department of Orthopedic Surgery Nara Medical University Department of Public Health Health Management and Policy Nara Medical University Faculty of Medicine Department of Biomedical Engineering Doshisha University Department of Arthroplasty and Regenerative Medicine Nara Medical University

AIM: To determine the effects of transplanting osteogenic matrix cell sheets and beta-tricalcium phosphate(TCP) constructs on bone formation in bone ***: Osteogenic matrix cell sheets were prepared from bone marrow stromal cells(BMSCs), and a porous TCP ceramic was used as a scaffold. Three experimental groups were prepared, comprised of TCP scaffolds(1) seeded with BMSCs;(2) wrapped with osteogenic matrix cell sheets; or(3) both. Constructs were implanted into a femoral defect model in rats and bone growth was evaluated by radiography, histology, biochemistry, and mechanical testing after 8 wk. RESULTS: In bone defects, constructs implanted with cell sheets showed callus formation with segmentalor continuous bone formation at 8 wk, in contrast to TCP seeded with BMSCs, which resulted in bone nonunion. Wrapping TCP constructs with osteogenic matrix cell sheets increased their osteogenic potential and resulting bone formation, compared with conventional bone tissue engineering TCP scaffolds seeded with BMSCs. The compressive stiffness(mean ± SD) values were 225.0 ± 95.7, 30.0 ± 11.5, and 26.3 ± 10.6 MPa for BMSC/TCP/Sheet constructs with continuous bone formation, BMSC/TCP/Sheet constructs with segmental bone formation, and BMSC/TCP constructs, respectively. The compressive stiffness of BMSC/TCP/Sheet constructs with continuous bone formation was significantly higher than those with segmental bone formation and BMSC/TCP ***: This technique is an improvement over current methods, such as TCP substitution, and is useful for hard tissue reconstruction and inducing earlier bone union in defects.

关键词： Bone marrow stromal cells Osteogenesis Bone regeneration Tissue engineering Calcium phosphate

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Inverse relationship between platelet Akt activity and hippocampal atrophy:A pilot case-control study in patients with diabetes mellitus

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World Journal of Clinical Cases 2024年第2期12卷 302-313页

作者： Haruhiko Tokuda Takamitsu Hori Daisuke Mizutani tomoyuki Hioki Kumi Kojima Takashi Onuma Yukiko Enomoto Tomoaki Doi Rie Matsushima-Nishiwaki Shinji Ogura Hiroki Iida Toru Iwama Takashi Sakurai Osamu Kozawa Department of Metabolic Research Research InstituteNational Center for Geriatrics and GerontologyObu 474-8511Japan Department of Clinical Laboratory National Center for Geriatrics and GerontologyObu 474-8511Japan Department of Pharmacology Gifu University Graduate School of MedicineGifu 501-1194Japan Department of Neurosurgery Gifu University Graduate School of MedicineGifu 501-1194Japan Department of Dermatology Central Japan International Medical CenterMinokamo 505-8510Japan Department of Anesthesiology and Pain Medicine Gifu University Graduate School of MedicineGifu 501-1194Japan Department of Emergency Medicine Faculty of MedicineUniversity of ToyamaToyama 930-0194Japan Department of Emergency and Disaster Medicine Gifu University Graduate School of MedicineGifu 501-1194Japan Research Institute National Center for Geriatrics and GerontologyObu 474-8511Japan

BACKGROUND Akt plays diverse roles in *** is involved in the pathogenesis of type 2 diabetes mellitus(T2DM),which is caused by insulin *** also plays a vital role in human platelet ***,the hippocampus is closely associated with memory and learning,and a decrease in hippocampal volume is reportedly associated with an insulin-resistant phenotype in T2DM patients without *** To investigate the relationship between Akt phosphorylation in unstimulated platelets and the hippocampal volume in T2DM *** Platelet-rich plasma(PRP)was prepared from the venous blood of patients with T2DM or age-matched *** pellet lysate of the centrifuged PRP was subjected to western blotting to analyse the phosphorylation of Akt,p38 mitogen-activated protein(MAP)kinase and glyceraldehyde 3-phosphate dehydrogenase(GAPDH).Phosphorylation levels were quantified by densitometric *** volume was analysed using a voxel-based specific regional analysis system for Alzheimer’s disease on magnetic resonance imaging,which proposes the Z-score as a parameter that reflects hippocampal *** The levels of phosphorylated Akt corrected with phosphorylated p38 MAP kinase were inversely correlated with the Z-scores in the T2DM subjects,whereas the levels of phosphorylated Akt corrected with GAPDH were ***,this relationship was not observed in the control *** These results suggest that an inverse relationship may exist between platelet Akt activation and hippocampal atrophy in T2DM *** findings provide insight into the molecular mechanisms underlying T2DM hippocampal atrophy.

关键词： Akt Platelet Hippocampal atrophy Magnetic resonance imaging Diabetes mellitus

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强磁场高温超导材料的制备与表征

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国外铁道机车与动车 2024年第4期 1-4,13页

作者： [日]tomoyuki AKASAKA 周贤全(译) 张霓虹(校) 不详

由于超导块材结构紧凑,可以产生强磁场,有可能作为磁场产生源应用于各种设备。用于磁体的超导块材,要求具有磁场强度高、均匀性好、稳定性好等特性。为此生产出MgB2(二硼化镁)和稀土基超导块材,并对其性能进行了评定。

关键词：高温超导性超导块材临界电流磁场

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Snowpeak白马陆地站

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城市环境设计 2024年第1期147卷 86-91页

作者：长谷川伦之梅川元一山本纮代田中克昌 IT Imaging

我们创建了一个名为“Snowpeak白马村陆地站”的户外娱乐设施，集商店、餐厅、咖啡馆和户外活动于一体，旨在刺激长野县白马村的经济发展。用地周围的树林里有木制拖车房住箱，游客可以享受露营和豪华野营的乐趣。大屋顶的造型设计旨在...

我们创建了一个名为“Snowpeak白马村陆地站”的户外娱乐设施，集商店、餐厅、咖啡馆和户外活动于一体，旨在刺激长野县白马村的经济发展。用地周围的树林里有木制拖车房住箱，游客可以享受露营和豪华野营的乐趣。大屋顶的造型设计旨在映射白马村三座山峰的轮廓。此外，还根据树枝和雪花的形状，使用小直径木材设计了一个木结构框架。利用类似篷布使用的织物将空间分割成不同区域，并在墙壁上排列了带有树皮的木制百叶窗，创造出室内却能体验“野外郊游”的空间感。

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Identification of telomere maintenance gene variations related to lung adenocarcinoma risk by genome-wide association and whole genome sequencing analyses

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Cancer Communications 2024年第2期44卷 287-293页

作者： Kouya Shiraishi Atsushi Takahashi Yukihide Momozawa Yataro Daigo Syuzo Kaneko Takahisa Kawaguchi Hideo Kunitoh Shingo Matsumoto Hidehito Horinouchi Akiteru Goto Takayuki Honda Kimihiro Shimizu Masahiro Torasawa Daisuke Takayanagi Motonobu Saito Akira Saito Yuichiro Ohe Shun-ichi Watanabe Koichi Goto Masahiro Tsuboi Katsuya Tsuchihara Sadaaki Takata Tomomi Aoi Atsushi Takano Masashi Kobayashi Yohei Miyagi Kazumi Tanaka Hiroyuki Suzuki Daichi Maeda Takumi Yamaura Maiko Matsuda Yoko Shimada Takaaki Mizuno Hiromi Sakamoto Teruhiko Yoshida Yasushi Goto Tatsuya Yoshida Taiki Yamaji Makoto Sonobe Shinichi Toyooka Kazue Yoneda Katsuhiro Masago Fumihiro Tanaka Megumi Hara Nobuo Fuse Satoshi S.Nishizuka Noriko Motoi Norie Sawada Yuichiro Nishida Kazuki Kumada Kenji Takeuchi Kozo Tanno Yasushi Yatabe Kuniko Sunami tomoyuki Hishida Yasunari Miyazaki Hidemi Ito Mitsuhiro Amemiya Hirohiko Totsuka Haruhiko Nakayama tomoyuki Yokose Kazuyoshi Ishigaki Toshiteru Nagashima Yoichi Ohtaki Kazuhiro Imai Ken Takasawa Yoshihiro Minamiya Kazuma Kobayashi Kenichi Okubo Kenji Wakai Atsushi Shimizu Masayuki Yamamoto Motoki Iwasaki Koichi Matsuda Johji Inazawa Yuichi Shiraishi Hiroyoshi Nishikawa Yoshinori Murakami Michiaki Kubo Fumihiko Matsuda Yoichiro Kamatani Ryuji Hamamoto Keitaro Matsuo Takashi Kohno Division of Genome Biology National Cancer Center Research InstituteTokyoJapan Department of Clinical Genomics National Cancer Center Research InstituteTokyoJapan Laboratory for Statistical Analysis Center for Integrative Medical SciencesRIKENYokohamaJapan Department of Genomic Medicine Research InstituteNational Cerebral and Cardiovascular CenterOsakaJapan Laboratory for Genotyping Development Center for Integrative Medical SciencesRIKENYokohamaJapan Center for Antibody and Vaccine Therapy Research HospitalInstitute of Medical ScienceThe University of TokyoTokyoJapan Department of Medical Oncology and Cancer Center and Center for Advanced Medicine against CancerShiga University of Medical ScienceOtsuJapan Division of Medical AI Research and Development National Cancer Center Research InstituteTokyoJapan Center for Genomic Medicine Graduate School of MedicineKyoto UniversityKyotoJapan Department of Medical Oncology Japanese Red Cross Medical CenterTokyoJapan Department of Thoracic Oncology National Cancer Center Hospital EastJapan Department of Thoracic Oncology National Cancer Center HospitalTokyoJapan Department of Cellular and Organ Pathology Graduate School of MedicineAkita UniversityAkitaJapan Department of Respiratory Medicine Tokyo Medical and Dental UniversityBunkyo-kuTokyoJapan Division of General Thoracic Surgery Department of SurgeryShinshu University School of MedicineMatsumotoNaganoJapan Department of General Surgical Science Division of General Thoracic SurgeryGunma University Graduate School of MedicineIntegrative Center of General SurgeryGunma University HospitalGunmaJapan Department of Gastrointestinal Tract Surgery Fukushima Medical University School of MedicineFukushimaJapan StaGen Co. LtdTokyoJapan Division of Thoracic Surgery National Cancer Center HospitalTokyoJapan Department of Thoracic Surgery National Cancer Center Hospital EastChibaJapan Division of Translational Informatics Exploratory Oncology Research and Clinical Trial Center(EPOC

Dear editor,Lung carcinoma is responsible for the highest fatal-ity rate among cancer-related deaths globally,with lung adenocarcinoma(LADC)emerging as the prevailing sub-type.

关键词： adenocarcinoma lung Lung

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Fabrication of luminescent patterns using aggregation-induced emission molecules by an electrolytic micelle disruption approach

聚集体(英文)

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聚集体(英文) 2023年第1期4卷 231-237页

作者： Yaqian Zhou Elena Villani tomoyuki Kurioka Yanyun Zhang Ikuyoshi Tomita Shinsuke Inagi Department of Chemical Science and Engineering School of Materials and Chemical TechnologyTokyo Institute of TechnologyYokohamaJapan PRESTO Japan Science and Technology Agency(JST)KawaguchiJapan

Herein,we report an electrochemical strategy that could control the location of aggregation-induced emission(AIE)molecules on patterned electrodes in a precise and facile way,producing photoluminescent and electrochemiluminescent patterns with a variety of colors.A micelle composed of electroactive surfactants was broken during the electrooxidation process,in which AIE molecules inside these micelles were released on patterned *** patterned electrodes were pretreated by not only metal,but also multifarious conducting polymers(CPs).An in-depth investigation clarified a correlation between the variety of CPs used as electrodes and the oxidation rate of the electroactive surfactant due to different catalytic performances of ***,combined with wireless and gradient features of bipolar electrochemistry,a gradient luminescent pattern was easily *** current studies suggest more abundant luminescent patterns using AIE luminophores can be developed by such an electrochemical method,in both of graphical shapes and emitting colors.

关键词： aggregation-induced emission bipolar electrochemistry catalysis conducting polymers electrochemiluminescence electrolytic micelle disruption

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Characteristics of In-Hospital Patients with Congenital Heart Disease Requiring Rapid Response System Activations: A Japanese Database Study

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Congenital Heart Disease 2022年第1期17卷 31-43页

作者： Taiki Haga tomoyuki Masuyama Yoshiro Hayashi Takahiro Atsumi Kenzo Ishii Shinsuke Fujiwara Department of Pediatric Critical Care Medicine Osaka City General HospitalOsaka534-0021Japan Department of Emergency and Critical Care Medicine Jichi Medical University Saitama Medical CenterSaitama330-8503Japan Department of Intensive Care Medicine Kameda Medical CenterChiba296-8602Japan Department of Emergency Medicine Seirei Hamamatsu General HospitalShizuoka430-8558Japan Department of Anesthesiology Intensive Care UnitFukuyama City HospitalHiroshima721-8511Japan Department of Emergency Medicine NHO Ureshino Medical CenterSaga843-0393Japan

Objectives:This study aimed to study the characteristics of in-hospital deterioration in patients with congenital heart disease who required rapid response system activation and identify risk factors associated with 1-month ***:We retrospectively analysed data from a Japanese rapid response system registry with 35 participating *** included consecutive patients with congenital heart disease who required rapid response system activation between January 2014 and March *** regression analyses were performed to examine the associations between 1-month mortality and other patient-specific ***:Among 9,607 patients for whom the rapid response system was activated,only 82(0.9%)had congenital heart *** few patients with congenital heart disease were being treated at the cardiology and cardiovascular surgery departments(12.3%and 9.9%,respectively).Moreover,the incidences of rapid-response events after intensive care unit discharge or surgery were low(6.8% and 12.2%,respectively).The most common reason for rapid response system activation was respiratory dysfunction(desaturation:35.4%,tachypnoea:25.6%,and new dyspnoea:19.5%).Rapid response system interventions and intensive care unit transfers were required for 65.9% and 20.7%of patients,*** mortality rate was 1.2% at the end of the rapid response system intervention and 11.0% after 1 ***,decreased respiratory rate and decreased heart rate at rapid response system activation were associated with increased 1-month *** adjusted odds ratio was 1.10(95% confidence interval 1.02–1.19)and 1.02(95% confidence interval,1.00–1.04) for respiratory rate and heart rate,***:Rapid response systems were rarely activated after cardiac surgery and intensive care unit discharge,which were situations with a high risk of sudden deterioration in patients with congenital heart ***,encouraging the use of the rapid response sys

关键词： Heart defects congenital emergencies clinical deterioration hospital rapid response team critical care This

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Research on Lightning Protection of Overhead Power Distribution Lines

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高电压技术 2013年第10期39卷 2317-2328页

作者： Shigeru Yokoyama tomoyuki Sato Graduate School of Engineering Shizuoka University Shizuoka 4228526 Japan Tohoku Electric Power Company Sendai 9808550 Japan

There are two major protective methods against lightning outages on overhead distribution *** is a surge arrester,and the other is an overhead ground *** surge arresters have rather constant effect regardless of the cause of the lightning *** the other hand,the effect of an overhead ground wire is quite different in two major causes,the direct lightning hit and the induced *** is sufficient to provide surge arresters with an interval of 300 m for protection against the induced overvoltage caused by the nearby lightning *** of an overhead ground wire together with surge arresters is effective for lightning protection against the direct lightning hit to a distribution *** of surge arresters is the popular outage when the outage caused by the insulation break decreases ***,the existence of nearby trees leads to the line break due to the side flash from a *** lightning protection equipment with ZnO arrester components is popular in *** of a pole transformer and application of an electromagnetic analysis method,such as FDTD method,to surge phenomena is considerably advanced.

关键词：架空配电线路防雷保护雷击感应过电压时域有限差分方法架空地线避雷器防护方法直接雷击

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Involvement of A5/A7 noradrenergic neurons and B2 serotonergic neurons in nociceptive processing:a fiber photometry study

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Neural Regeneration Research 2022年第4期17卷 881-886页

作者： Shunpei Moriya Akira Yamashita Daiki Masukawa Junichi Sakaguchi Yoko Ikoma Yoshimune Sameshima Yuki Kambe Akihiro Yamanaka tomoyuki Kuwaki Department of Physiology Kagoshima University Graduate School of Medical and Dental ScienceKagoshimaJapan Department of Molecular Pharmacologyand Neurobiology Yokohama City University Graduate School of MedicineYokohamaJapan Department of Pharmacology Kagoshima University Graduate School of Medical and Dental ScienceKagoshimaJapan Research Institute of Environmental Medicine Nagoya UniversityNagoyaJapan

In the central nervous system,the A6 noradrenaline(NA)and the B3 serotonin(5-HT)cell groups are well-recognized players in the descending antinociceptive system,while other NA/5-HT cell groups are not well characterized.A5/A7 NA and B25-HT cells project to the spinal horn and form descending *** recorded G-Ca MP6 green fluorescence signal intensities in the A5/A7 NA and the B25-HT cell groups of awake mice in response to acute tail pinch stimuli,acute heat stimuli,and in the context of a non-noxious control test,using fiber photometry with a calcium imaging *** first introduced G-Ca MP6 in the A5/A7 NA or B25-HT neuronal soma,using transgenic mice carrying the tetracycline-controlled transactivator transgene under the control of either a dopamineβ-hydroxylase or a tryptophan hydroxylase-2 promoters and by the site-specific injection of adeno-associated virus(AAV-Tet O(3 G)-G-Ca MP6).After confirming the specific expression patterns of G-Ca MP6,we recorded G-Ca MP6 green fluorescence signals in these sites in awake mice in response to acute nociceptive stimuli.G-Ca MP6 fluorescence intensity in the A5,A7,and B2 cell groups was rapidly increased in response to acute nociceptive stimuli and soon after,it returned to baseline fluorescence *** was not observed in the non-noxious control *** results indicate that acute nociceptive stimuli rapidly increase the activities of A5/A7 NA or B25-HT neurons but the non-noxious stimuli do *** present study suggests that A5/A7 NA or B25-HT neurons play important roles in nociceptive processing in the central nervous *** suggest that A5/A7/B2 neurons may be new therapeutic *** performed procedures were approved by the Institutional Animal Use Committee of Kagoshima University(MD17105)on February 22,2018.

关键词： A5 NA neurons A7 NA neurons B25-HT neurons DBH-tTA mice fiber photometry G-CaMP6 mCherry monoaminergic signaling nociception TPH-t TA mice

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