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Comparative Transcriptional Profiling and Preliminary Study on Heterosis Mechanism of Super-Hybrid Rice

Molecular Plant 2010年第6期3卷 1012-1025页

作者： Gui-Sheng Song Hong-Li Zhai Yong-Gang Peng Lei Zhang Gang Wei Xiao- Ying Chen Yu-Guo Xiao Lili Wang Yue-Jun Chen Bin Wu Bin Chen Yu Zhang Hua Chen Xiu-Jing Feng Wan-Kui Gong Yao Liu Zhi-Jie Yin Feng Wang Guo-Zhen Liu Hong-Lin Xu Xiao-Li Wei Xiao-Ling Zhao Pieter B.F. Ouwerkerk thomas Hankemeier Theo Reijmers Rob van der Heijden Cong-Ming Lu Mei Wang Jan van der Greef Zhen Zhu State Key Laboratory of Plant Genomics Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China National Plant Gene Research Center (Beijing) Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China State Key Laboratory of Genetic Engineering Institute of Genetics School of Life Sciences Fudan University Shanghai 200433 China Key Laboratory of Photosynthesis and Environmental Molecular Physiology Institute of Botany Chinese Academy of Sciences Beijing 100093 China Fujian Province Key Laboratory of Genetic Engineering for Agriculture Fujian Academy of Agricultural Sciences Fuzhou 350003 China Beijing Institute of Genomics Chinese Academy of Sciences Beijing 101300 China Institute of Biology Leiden University Sylvius Laboratory Wassenaarseweg 72 2333 BE Leiden The Netherlands Leiden/Amsterdam Center for Drug Research Center for Medical Systems Biology Leiden University Einsteinweg 5 2500 RA Leiden The Netherlands SU BioMedicine and TNO Quality of Life Utrechtseweg 48 3700 AJ Zeist The Netherlands

Heterosis is a biological phenomenon whereby the offspring from two parents show improved and superior performance than either inbred parental lines. Hybrid rice is one of the most successful apotheoses in crops utilizing heterosis. Transcriptional profiling of F1 super-hybrid rice Liangyou-2186 and its parents by serial analysis of gene expression （SAGE） revealed 1183 differentially expressed genes （DGs）, among which DGs were found significantly enriched in pathways such as photosynthesis and carbon-fixation, and most of the key genes involved in the carbon-fixation pathway exhibited up-regulated expression in F1 hybrid rice. Moreover, increased catabolic activity of corresponding enzymes and photosynthetic efficiency were also detected, which combined to indicate that carbon fixation is enhanced in F1 hybrid, and might probably be associated with the yield vigor and heterosis in super-hybrid rice. By correlating DGs with yield-related quantitative trait loci （QTL）, a potential relationship between differential gene expression and phenotypic changes was also found. In addition, a regulatory network involving circadian-rhythms and light signaling pathways was also found, as previously reported in Arabidopsis, which suggest that such a network might also be related with heterosis in hybrid rice. Altogether, the present study provides another view for understanding the molecular mechanism underlying heterosis in rice.

关键词： Heterosis super-hybrid rice transcriptional profiling photosynthesis carbon fixation regulatory network.

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A Spherical Atom Model of Helium Based on Well-Defined Electron Trajectories

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Journal of Applied Mathematics and Physics 2022年第6期10卷 1998-2014页

作者： thomas Allmendinger Independent Scholar Glattbrugg (close to Zurich) Switzerland

The present approach is an advancement of the author’s former attempts to develop an atom model of Helium with well-defined electron trajectories. Thus it calls in question the traditional quantum mechanics which assume—in contrast and as a consequence of Heisenberg’s uncertainty principle—electronic probabilities of presence. Its basic idea consists of the assumption that the motions of the two electrons are influenced by their spins exhibiting the value h/2π, but in two different ways: on the one hand, one spin induces a rotation;and on the other hand, the other spin induces a harmonic oscillation. A second important relation is given by the fact that the retroactive force of the oscillation process is due to the centrifugal force when the process runs along the surface of a sphere, whereas in usual oscillation processes—such as the one of a spring pendulum—it is due to a permanent shift between potential and kinetic energy. Therefore, in the present case, the potential energy remains constant since the distance between the nucleus and the—diametrically positioned—electrons remains constant. Considering these two conditions and the usual physical relations such as Coulomb attraction, centrifugal force, and the conservation laws of the angular momentum and of the energy, it was possible to compute the respective key values. Thereby, the deflection of the oscillation angle ψ = 45˚is remarkable. Finally, the process is described using a Cartesian coordinate system with z as the rotation axis, a variable oscillation distance d and variable rotation velocities rrot. Thereby, the projections onto the x-axis and on the y-axis are not identically equal, leading to an elliptic projection shape. Thus this system is anisotropic, in contrast to the isotropic array of the conventional quantum mechanics according to Schrödinger, where the 1s-orbital is spherically symmetrical. This anisotropy explains the existence of interatomic van der Waals forces, which

关键词： 3D-Atom-Model Electron-Spin Electron Wave Confutation of the Traditional Quantum Mechanics according to Schrödinger and Heisenberg

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Two-dimensional ferroelectrics from high throughput computational screening

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npj Computational Materials 2023年第1期9卷 1901-1911页

作者： Mads Kruse Urko Petralanda Morten N.Gjerding Karsten W.Jacobsen Kristian S.Thygesen thomas Olsen CAMD Department of PhysicsTechnical University of DenmarkDK-2800 Kongens LyngbyDenmark

We report a high throughput computational search for two-dimensional ferroelectric *** starting point is 252 pyroelectric materials from the computational 2D materials database(C2DB)and from these we identify 63 *** particular we find 49 materials with in-plane polarization,8 materials with out-of-plane polarization and 6 materials with coupled in-plane and out-of-plane *** of the known 2D ferroelectrics are recovered by the screening and the far majority of the predicted ferroelectrics are known as bulk van der Waals bonded compounds,which makes them accessible by direct *** roughly 25%of the materials we find a metastable state in the non-polar structure,which may imply a first order transition to the polar ***,we list the magnetic pyroelectrics extracted from the C2DB and focus on the case of VAgP2Se6,which exhibits a three-state switchable polarization vector that is strongly coupled to the magnetic excitation spectrum.

关键词： materials polarization plane

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公司交通政策的最佳实践——以比利时为例

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城市交通 2012年第4期10卷 79-90页

作者： Laurent van Malderen Bart Jourquin Isabelle thomas thomas vanoutrive Ann Verhetsel Frank Witlox 张宇天主教鲁汶大学鲁汶管理学院天主教鲁汶大学运筹学与计量经济学中心及地理学院安特卫普大学交通与区域经济系根特大学地理系中国城市规划设计研究院北京100037

公司是通勤交通的主要创造者,在与交通相关的讨论中,公司扮演着重要角色。近几年,公司提出了多种政策措施,以改善员工的出行条件,但其愿景与行动通常被研究文献所忽视。为了讨论比利时公司在交通政策方面的最佳实践,分析了现有研究成果... 详细信息

公司是通勤交通的主要创造者,在与交通相关的讨论中,公司扮演着重要角色。近几年,公司提出了多种政策措施,以改善员工的出行条件,但其愿景与行动通常被研究文献所忽视。为了讨论比利时公司在交通政策方面的最佳实践,分析了现有研究成果和比利时的两次大规模通勤交通调查。首先,对公司进行分类,以便确定在推广替代交通方式方面受员工欢迎的公司。然后,通过量化分析确定交通政策的最佳实践。结果显示,最佳替代交通方式取决于公司特征;自行车交通最适合小型工作场所,大型工作场所和位于建筑密集区或市中心的公司则更适合推广公共交通;在公司交通管理中,财务激励、设施提供、信息传播和停车管理均起重要作用。

关键词：通勤交通雇主交通计划可持续交通

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Enhanced π-π Interactions Between a C_(60) Fullerene and a Buckle Bend on a Double-Walled Carbon Nanotube

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Nano Research 2010年第2期3卷 92-97页

作者： Sandeep Gorantla Stanislav Avdoshenko Felix Börrnert Alicja Bachmatiuk Maria Dimitrakopoulou Franziska Schäffel Ronny Schönfelder Jürgen thomas thomas Gemming Jamie HWarner Gianaurelio Cuniberti Jürgen Eckert Bernd Büchner Mark H.Rümmeli IFW Dresden P.O.Box 270116D-01171 DresdenGermany Institute for Materials Science and Max Bergmann Center of Biomaterials Dresden University of TechnologyDresden 01062Germany Department of Materials University of OxfordParks Rd.Oxford OX13PHUnited Kingdom

In situ low-voltage aberration corrected transmission electron microscopy(TEM)observations of the dynamic entrapment of a C_(60) molecule in the saddle of a bent double-walled carbon nanotube is *** fullerene interaction is non-covalent,suggesting that enhancedπ-πinteractions(van der Waals forces)are *** molecular dynamics calculations confirm that the increased interaction area associated with a buckle is sufficient to trap a ***,they show hopping behavior in agreement with our experimental *** findings further our understanding of carbon nanostructure interactions,which are important in the rapidly developing field of low-voltage aberration corrected TEM and nano-carbon device fabrication.

关键词： Carbon nanotubes fullerenes low-voltage transmission electron microscopy molecule trap

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An implantable,wireless,battery-free system for tactile pressure sensing

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Microsystems & Nanoengineering 2023年第5期9卷 309-320页

作者： Lin Du Han Hao Yixiao Ding Andrew Gabros thomas C.E.Mier Jan van der Spiegel Timothy H.Lucas Firooz Aflatouni Andrew G.Richardson Mark G.Allen Department of Electrical and Systems Engineering School of Engineering and Applied ScienceUniversity of PennsylvaniaPhiladelphiaPAUSA Department of Neurosurgery Perelman School of MedicineUniversity of PennsylvaniaPhiladelphiaPAUSA Departments of Neurosurgery and Biomedical Engineering Ohio State UniversityColumbusOHUSA

The sense of touch is critical to dexterous use of the hands and thus an essential component of efforts to restore hand function after amputation or *** systems have addressed this goal with wearable tactile ***,such wearable sensors are suboptimal for neuroprosthetic systems designed to reanimate a patient’s own paralyzed ***,we developed an implantable tactile sensing system intended for subdermal *** system is composed of a microfabricated capacitive pressure sensor,a custom integrated circuit supporting wireless powering and data transmission,and a laser-fused hermetic silica *** miniature device was validated through simulations,benchtop assessment,and testing in a primate *** sensor implanted in the fingertip accurately measured applied skin forces with a resolution of 4.3 *** output from this novel sensor could be encoded in the brain with microstimulation to provide tactile *** broadly,the materials,system design,and fabrication approach establish new foundational capabilities for various applications of implantable sensing systems.

关键词： system battery fused

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Practical handling skills during road transport of fattening pigs from farm to slaughterhouse: A brief review

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Agricultural Sciences 2013年第12期4卷 756-761页

作者： Bert Driessen Ester Peeters Jos van Thielen Sanne van Beirendonck Department of Animal Welfare Federal Government Service Brussels Belgium Department of Bioengineering Technology KU Leuven | Thomas More Geel Belgium

The transport of fattening pigs is characterized by a strong human-animal interaction. Consequent handling is important because of animal welfare, meat quality and matching economic consequences. During road transport, human impact can be divided in different steps: 1) driving pigs from the pens via an alley to the trailer, 2) loading, 3) actual transport, 4) unloading to the lairage, and 5) the final phase driving pigs to the stunning. An inadequate design and a poor condition of the facilities will negatively affect the ease of handling pigs. Because of the consequences, acute stress during transport and slaughter should be minimized by acting on the education of people, on equipment and on preparation of animals for the journey. Education programs have to be repeated regularly so that knowledge can be refreshed.

关键词： Handling Pigs Transport Slaughterhouse Training

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Design of molecule-based magnetic conductors

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Nano Research 2014年第12期7卷 1832-1842页

作者： Naureen Akhtar Graeme R. Blake Roberto Felici Heinz Amenitsch thomas T. M. Palstra Petra Rudolf Zernike Institute for Advanced Materials University of Groningen Nijenborgh 4 NL-9747AG Groningen The Netherlands ESRF BP 220 F-38043 Grenoble Cedex 9 France Institute of Inorganic Chemistry Graz University of Technology Stremayerg. 9/IV A-8010 Graz Austria

Enabling the use of rationally designed thin films in technological devices is a recognized goal in materials science. However, constructing such thin films using highly ordered supramolecular architectures with well-controlled size and growth direction has remained an elusive target. Here, we introduce a layer-by- layer protocol to grow hybrid thin films of molecule-based magnetic conductors comprising arachidic acid and donor bis（ethylenedioxy）tetrathiafulvalene （BEDO-TTF） as the organic component and Cu/Gd complexes as the inorganic component. The construction of layered hybrid thin films was achieved at ambient conditions by employing the Langmuir-Blodgett method, which provides good control over film thickness and packing of molecules in the monolayer. As demonstrated by X-ray diffraction, these films are crystalline with distinct organic and inorganic sublattices, where the BEDO-TTF molecular layer is interfaced with the inorganic layer. Due to the flexibility of the Langmuir-Blodgett deposition technique, this result indicates a route toward the preparation of well-ordered films with various functionalities, determined by the choice of the inorganic compound that is combined with the π-unit of BEDO-TFF. Moreover, the ability to deposit films on a variety of substrates establishes the potential for lower-cost device fabrication on inexpensive substrates.

关键词： molecule-based materials self-assembly ordered heterostructures multifunctionality ultra-thin films

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HPLC/LTQ-FTMS用于白参中的丙二酰基人参皂苷的研究

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世界科学技术-中医药现代化 2009年第1期11卷 190-194页

作者：孔宏伟王梅 Rob van der Heijden Jan van der Greef thomas Hankemeier 许国旺中国科学院大连化学物理研究所代谢组学研究中心大连116023 荷兰应用科学研究组织(TNO)SU生物药品公司荷兰应用科学研究组织(TNO)生命科学部荷兰莱顿大学荷兰国家代谢组学中心

在15mM甲酸铵作为流动相添加剂条件下,采用HPLC/LTQ-FTMS实现了白参样品中酸性人参皂苷的优化分离。根据高分辨质谱、多级质谱信息,结合保留规律验证,在白参萃取液中共检测和识别了八种丙二酰基人参皂苷,包括丙二酰基人参皂苷Rg1、Re、R... 详细信息

在15mM甲酸铵作为流动相添加剂条件下,采用HPLC/LTQ-FTMS实现了白参样品中酸性人参皂苷的优化分离。根据高分辨质谱、多级质谱信息,结合保留规律验证,在白参萃取液中共检测和识别了八种丙二酰基人参皂苷,包括丙二酰基人参皂苷Rg1、Re、Rf、Rb1、Rc、Rb2、Rb3和Rd。

关键词： HPLC/LTQ—FTMS 丙二酰基人参皂苷白参甲酸铵

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RosBREED:bridging the chasm between discovery and application to enable DNA-informed breeding in rosaceous crops

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Horticulture Research 2020年第1期7卷 527-549页

作者： Amy F.Iezzoni Jim McFerson James Luby Ksenija Gasic vance Whitaker Nahla Bassil Chengyan Yue Karina Gallardo Vicki McCracken Michael Coe Craig Hardner Jason D.Zurn Stan Hokanson Eric van de Weg Sook Jung Dorrie Main Cassia da Silva Linge Stijn vanderzande thomas M.Davis Lise L.Mahoney Chad Finn Cameron Peace Michigan State University East LansingMI 48824USA Washington State University WenatcheeWA 98801USA University of Minnesota St.PaulMN 55108USA Clemson University ClemsonSC 29634USA University of Florida WimaumaFL 33598USA USDA-ARS CorvallisOR 97333USA Washington State University PuyallupWA 98371USA Washington State University PullmanWA 99164USA Cedar Lake Research Group PortlandOR 97215USA University Queensland BrisbaneQLDAustralia Wageningen University and Research 6700 AA WageningenThe Netherlands University of New Hampshire DurhamNH 03824USA

The Rosaceae crop family(including almond,apple,apricot,blackberry,peach,pear,plum,raspberry,rose,strawberry,sweet cherry,and sour cherry)provides vital contributions to human well-being and is economically significant across the *** 2003,industry stakeholder initiatives prioritized the utilization of genomics,genetics,and breeding to develop new cultivars exhibiting both disease resistance and superior horticultural ***,rosaceous crop breeders lacked certain knowledge and tools to fully implement DNA-informed breeding—a“chasm”existed between existing genomics and genetic information and the application of this knowledge in *** RosBREED project(“Ros”signifying a Rosaceae genomics,genetics,and breeding community initiative,and“BREED”,indicating the core focus on breeding programs),addressed this challenge through a comprehensive and coordinated 10-year effort funded by the USDA-NIFA Specialty Crop Research *** was designed to enable the routine application of modern genomics and genetics technologies in *** crop breeding programs,thereby enhancing their efficiency and effectiveness in delivering cultivars with producer-required disease resistances and market-essential horticultural *** review presents a synopsis of the approach,deliverables,and impacts of RosBREED,highlighting synergistic global collaborations and future *** technologies and tools developed are described,including genome-wide scanning platforms and DNA diagnostic *** of DNA-informed breeding use by project participants are presented for all breeding stages,including pre-breeding for disease resistance,parental and seedling selection,and elite selection *** chasm is now bridged,accelerating rosaceous crop genetic improvement.

关键词： breeding cultivar crops

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