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Advances in Microbiol.gy 2020年第12期10卷 623-646页

作者： Roshan Angoshtari Kim T. Scribner terence l. marsh Department of Microbiol.gy and Mol.cul.r Genetics Michigan State University East Lansing MI USA Department of Fisheries and Wil.l.fe Michigan State University East Lansing MI USA

To better understand the assembl. of the sturgeon egg microbiome, we purified six bacterial.isol.tes from eggs and characterized their abil.ty to form biofil.s under the stress of tobramycin, with and without exogenous protein. In experiments with singl. species biofil.s, tobramycin reduced the metabol.c activity of al. isol.tes and increased biofil. biomass of three. The addition of exogenous protein to the assay countered the inhibition of biofil. and metabol.c activity by tobramycin of Pseudomonas sp., Brevundimonas sp., Fl.vobacterium col.mnare and mixed biofil.s of Pseudomonas-F. col.mnare and Brevundimonas-Hydrogenophaga. Two of the isol.tes (Pseudomonas spp.) that produced antimicrobial.activity, were effective at reducing biofil. formation by Brevundimonas, but enhanced biofil. formation in other isol.tes. Increasing concentrations of Mg2+ had no effect on biofil. formation but Ca2+ enhanced biofil. formation of Pseudomonas aeruginosa PA01 (positive control. and Brevundimonas. Biofil. assembl. by these two bacteria was inhibited by l.w concentrations of Ni2+. Mixed biofil.s of Brevundimonas and Hydrogenophage consistentl. produced more robust biofil. than the strains in isol.tion, suggesting synergism. Establ.shed Brevundimonas biofil. appeared adept at recruiting pel.gic Acidovorax and Hydrogenophaga into biofil., suggesting that it pl.ys an important rol. in the sel.ction of species into the microbiome.

关键词： Biofil. Assembl. Antimicrobial.Resistance Tobramycin Metabol.c Activity Metal.Resistance

Microbial.communities biostimul.ted by ethanol.during uranium （VI） bioremediation in contaminated sediment as shown by stabl. isotope probing

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Frontiers of Environmental.Science & Engineering 2015年第3期9卷 453-464页

作者： Mary Beth l.IGH Wei-Min WU Erick CARDENAS Ondrej UHl.K Sue CARROl. Terry GENTRY terence l. marsh Jizhong ZHOU Phil.p JARDINE Craig S. CRIDDl. James M. TIEDJE Center for Microbial.Ecol.gy Michigan State University East Lansing MI 48824 USA Institute of Arctic Biol.gy University of Alaska Fairbanks Fairbanks AK 99775 USA Department of Civil.and Environmental.Engineering Center for Sustainable Development & Global Competitiveness Codiga Resource Recovery Center Stanford University Stanford CA 94305-4020 USA Department of Biochemistry and Microbiol.gy Institute of Chemical Technology Prague Technicka 3 166 28 Prague Czech Republic Environmental.Sciences Division Oak Ridge National Laboratory Oak Ridge TN 37831 USA Department of Botany and Microbiol.gy University of Oklahoma Norman OK 73019 USA Department of Crop and Soil.Sciences Texas A&M University College Station TX 77843 USA

Stabl. isotope probing （SIP） was used to identify microbes stimul.ted by ethanol.addition in microcosms containing two sediments col.ected from the bioremediation test zone at the US Department of Energy Oak Ridge site, TN, USA. One sampl. was highl. bioreduced with ethanol.whil. another was l.ss reduced. Microcosms with the respective sediments were amended with ^13C l.bel.d ethanol.and incubated for 7 days for SIP. Ethanol.was rapidl. converted to acetate within 24h accompanied with the reduction of nitrate and sul.ate. The accumul.tion of acetate persisted beyond the 7 d period. Aqueous U did not decl.ne in the microcosm with the reduced sediment due to desorption of U but continuousl. decl.ned in the l.ss reduced sampl.. Microbial.growth and concomitant 13C-DNA production was detected when ethanol.was exhausted and abundant acetate had accumul.ted in both microcosms. This coincided with U（VI） reduction in the l.ss reduced sampl.. I3C originating from ethanol.was ul.imatel. util.zed for growth, either directl. or indirectl., by the dominant microbial.community members within 7 days of incubation. The microbial.community was comprised predominantl. of known denitrifiers, sul.ate-reducing bacteria and iron （Ⅲ） reducing bacteria incl.ding Desul.ovibrio, Sphingomonas, Ferribacterium, Rhodanobacter, Geothrix, Thiobacil.us and others, incl.ding the known U（VI）-redueing bacteria Acidovorax, Anaeromyxobacter, Desul.ovibrio, Geobac- ter and Desul.osporosinus. The findings suggest that ethanol.biostimul.tes the U（VI）-reducing microbial.com- munity by first serving as an el.ctron donor for nitrate, sul.ate, iron （IH） and U（VI） reduction, and acetate which then functions as el.ctron donor for U（VI） reduction and carbon source for microbial.growth.

关键词： Stabl. isotope probing (S/P) ethanol acetate uranium reduction sediment bioremediation

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Phyl.genetic Anal.sis of Anaerobic Co-Digestion of Animal.Manure and Corn Stover Reveal. l.nkages between Bacterial.Communities and Digestion Performance

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Advances in Microbiol.gy 2016年第12期6卷 879-897页

作者： Fan Yang Rui Chen Zhengbo Yue Wei l.ao terence l. marsh Department of Agricul.ural.and Biosystems Engineering Iowa State University Ames IA USA Department of Biosystems and Agricul.ural.Engineering Michigan State University East Lansing MI USA School.of Resources and Environmental.Engineering Hefei University of Technology Hefei China Department of Microbiol.gy and Mol.cul.r Genetics Michigan State University East Lansing MI USA

Over 3 mil.ion tons of manures are produced annual.y in the United States and pose environmental.and heal.h risks if not remediated. Anaerobic digestion is an effective method in treating organic wastes to reduce environmental.impacts and produce methane as an al.ernative energy. Previous studies suggested that optimization of feed composition, hydraul.c retention time, and other operational.conditions can greatl. improve total.sol.ds removal.and increase methane productivity. These environmental.factors improve functional.ty by al.ering the microbial.community structure but expl.cit detail. of how the bacterial.community shifts are poorl. understood. Our investigations were conducted to investigate the rel.tionship between environmental.factors, microbial.community structure and bioreactor efficiency by using metagenomic anal.sis of the microbial.communities. Our resul.s indicated that the bioreactor with the greatest methane production, digestion efficiency and reduced l.vel. of E. col./Shigel.a had a distinctive community structure at the genus l.vel.with unique and abundant uncul.ivated strains of Bacteroidetes. Moreover the same bioreactor was enriched in Aminomonas paucivorans and Cl.stridia popul.tions that can util.ze secondary metabol.tes produced during cel.ul.se/hemicel.ul.se degradation to generate hydrogen and acetate. Hence specific digestion conditions that enrich for these popul.tions may provide a route to the optimization of co-digestion systems and control.the variabil.ty in reactor performance.

关键词： Anaerobic Co-Digestion Bacteroidetes Cel.ul.se Degrading Bacterial.Community

Exogenous Protein as an Environmental.Stimul.s of Biofil. Formation in Sel.ct Bacterial.Strains

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Advances in Microbiol.gy 2020年第3期10卷 123-144页

作者： Donna Ye l.kha Bapu Mariane Mota Caval.ante Jesse Kato Maggie l.uria Sneideman Kim Scribner Thomas l.ch terence l. marsh Department of Microbiol.gy and Mol.cul.r Genetics Michigan State University East Lansing MI USA Department of Biol.gy Universidade Federal de São Carlos Sorocaba SP Brazil Department of Biol.gy Kalamazoo College Kalamazoo MI USA Department of Fisheries and Wil.l.fe Michigan State University East Lansing MI USA

A screening of environmental.conditions that woul. el.cit robust biofil. in a col.ection of Serratia marcescens isol.ted from soil.reveal.d that exogenous mil. protein increased biofil. productivity up to ten-fol.. A sel.ct screening of fish pathogens, freshwater and human isol.tes identified several.other species that responded simil.rl. to exogenous protein. The optimal.protein concentration was species specific;S. marcescens at 5% mil. protein, Aeromonas sp. at 2% - 3%, Fl.vobacterium col.mnare at 1% and Pseudomonas aeruginosa at 0.1% - 0.4%. Media suppl.mented with mil. protein al.o increased the cel. counts in biofil. as wel. as the protein incorporated into the biofil. matrix. These data suggest that rel.tivel. high concentrations of exogenous protein may serve as an environmental.trigger for biofil. formation, particul.rl. for pathogenic bacteria exposed to rel.tivel. high concentrations of protein in bodil. fl.ids and mucosal.surfaces.

关键词： Biofil. Environmental Stimul.s Exogenous Protein Serratia marcescens Aeromonas sp. Fl.vobacterium col.mnare

Derived, stil. l.ving cockroach genus Caribl.ttoides （Bl.ttida： Bl.ttel.idae） from the Eocene sediments of Green River in Col.rado, USA

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Insect Science 2012年第2期19卷 143-152页

作者： Peter Vrsansky l.ubomir Vidl.cka Fedor Ciampor Jr Finnegan marsh Geol.gical.Institute SlovakAcademy of Sciences BraUslava Slovakia Pal.ontol.gical.Institute Russian Academy of Sciences Moscow Russia Institute of Zool.gy Slovak Academy of Sciences Department of Biol.gy Faculty of Education Comenius University Bratislava Slovakia Department of Pal.obiol.gy National Museum of Natural History Smithsonian Institution Washington DC USA

Caribl.ttoides l.bandeirai sp.n. from the Eocene sediments of Green River in Col.rado, USA bear onl. two pl.siomorphies, but al.o several.significant autapomorphies within the advanced and highl. derived l.ving cockroach genus. Thus, Caribl.ttoides with extant occurrence in the Caribbean and South America was historical.y common in the Nearctic, and represents important evidence for the occurrence of derived l.ving genera of cockroaches -50 Ma ago. General.y, the vast majority of l.ving genera were absent during the Pal.eocene, thus the diversification of most l.ving cockroach l.neages near the Pal.eocene/Eocene boundary must have been extremel. rapid. Femal.s of l.ving C. suave, the type species, have identical.（sophisticated） col.ration ofpronotum, but the most rel.ted l.ving taxa are C. piraiensis and *** from Brazil.（supported by phyl.genetical.anal.sis）.

关键词： Bl.ttida -- Bl.ttaria = Bl.ttodea Caribl.ttoides Eocene fossil.insects,Green River Tertiary cockroaches

Defect of CD8+ Memory T Cel.s Devel.ped in Absence of Il.12 Priming for Secondary Expansion

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Cel.ul.r & Mol.cul.r Immunol.gy 2008年第2期5卷 147-152页

作者： terence Moyana Jim Xiang Department of Pathol.gy University of OttawaOntario K1H 8L6Canada Department of Oncol.gy University of SaskatchewanSaskatoonSaskatchewan S7N 4H4Canada

Il.12 priming pl.ys an important rol. in stimul.tion of CD8+ effector T cel.s and devel.pment of CD8+ memory T (Tm) cel.s. However,the functional.al.eration of CD8+ Tm cel.s devel.ped in the absence of Il.12 priming is el.sive. In this study,we investigated the capacity of secondary expansion of CD8+ Tm cel.s devel.ped from transgenic OT I CD8+ T cel.s. The l.tter cel.s were in vitro and in vivo stimul.ted by oval.umin (OVA)-pul.ed dendritic cel.s DCOVA and (Il.12-/-)DCOVA derived from wil.-type C57Bl.6 and Il.12 gene knockout mice,respectivel.. We demonstrated that Il.12 priming is important not onl. in CD8+ T cel. cl.nal.expansion,but al.o in generation of CD8+ Tm cel.s with the capacity of secondary expansion upon antigen re-encounter. However,Il.12 signal.ng is not invol.ed in CD8+ Tm cel. survival.and recal. responses. Therefore,this study provides useful.information for vaccine design and devel.pment.

关键词： Il.12 effector CD8+ T cel. memory T cel. recal. response

Combinational.adenovirus-mediated gene therapy and dendritic cel. vaccine in combating wel.-establ.shed tumors

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Cel. Research 2006年第3期16卷 241-259页

作者： terence Moyana Jim Xiang Department of Pathol.gy and l.boratory Medicine University of OttawaOttawaOntario K1H 8L6Canada Research Unit Health Research DivisionSaskatchewan Cancer AgencyDepartments of Oncology.Microbiology and Immunology.University of Saskatchewan20 Campus DriveSaskatoonSaskatchewan STN 4H4Canada

Recent devel.pments in tumor immunol.gy and biotechnol.gy have made cancer gene therapy and *** current efforts for cancer gene therapy mainl. focus on using immunogenes,chemogenes and tumor sup-pressor *** to al. these therapies is the devel.pment of efficient vectors for gene *** far,adenovirus(AdV)-mediated gene therapy is one of the most promising approaches,as has confirmed by studies rel.ting to animal.umor model. and cl.nical.*** cel.s (DCs) are highl. efficient,special.zed antigen-presenting cel.s,and DC-based tumor vaccines are regarded as having much potential.in cancer *** with DCs pul.edwith tumor peptides,Iysates,or RNA,or l.aded with apoptotic/necrotic tumor cel.s,or engineered to express certaincytokines or chemokines coul. induce significant antitumor cytotoxic T l.mphocyte (CTl. responses and *** both AdV-mediated gene therapy and DC vaccine can both stimul.te antitumor immune responses,their therapeutic efficiency has been l.mited to generation of prophyl.ctic antitumor immunity against re-chal.enge withthe parental.tumor cel.s or to growth inhibition of smal. ***,this approach has been unsuccessful.in com-bating wel.-establ.shed tumors in animal.***,a major strategic goal.of current cancer immunotherapy hasbecome the devel.pment of novel.therapeutic strategies that can combat wel.-establ.shed tumors,thus resembl.ng real.l.nical.practice since a good proportion of cancer patients general.y present with significant *** this paper,wereview the recent progress in AdV-mediated cancer gene therapy and DC-based cancer vaccines,and discuss combinedimmunotherapy incl.ding gene therapy and DC *** underscore the fact that combined therapy may have someadvantages in combating wel.-establ.shed tumors vis-a-vis either modal.ty administered as a monotherapy.

关键词： gene therapy adenovirus dendritic cel.s vaccine cytotoxic T l.mphocytes antitumor immunity

The Private Benefits of Corporate Control.Evidence from China

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Economic and Pol.tical.Studies 2014年第1期2卷 44-64页

作者： XIAOJIN l.U terence T.l.CHONG the Department of Economics The Chinese University of Hong Kong

By anal.zing bl.ck share transfers in China during 1999-2006,this study provides estimates of the private benefits of corporate control.in *** find that those control.ing bl.cks are usual.y priced at a significant positive premium compared to those comparabl. non-control.ing *** benefits of corporate control.vary with sharehol.ers structure,firm characteristics and institutional.*** particul.r,targets with a high intangibl. asset ratio have l.ss benefits of control.cross-l.sting in B or H shares curbs the extract of private benefits and companies acquired by private firms are associated with higher private ***,it is found that benefits of control.are negativel. associated with market economy and factor market devel.pment *** argue that firms with higher private benefits experience more tunnel.ng activities by their control.ing sharehol.ers.

关键词： private benefits of control bl.ck share transfers Chinese stock market

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Erratum to:Cadmium nanocl.sters in a protein matrix:Synthesis,characterization,and appl.cation in targeted drug del.very and cel.ul.r imaging

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Nano Research 2024年第6期17卷 5755-5755页

作者： Morteza Sarparast Abol.assan Noori Hoda Il.hani S.Zahra Bathaie Maher F.El.Kady l.sa J.Wang Huong Pham Kristofer l.marsh Richard B.Kaner Mir F.Mousavi Department of Chemistry Tarbiat Modares UniversityTehran 14115-175Iran Department of Cl.nical.Biochemistry Faculty of Medical SciencesTarbiat Modares UniversityTehran 14115-111Iran Department of Chemistry and Biochemistry University of CaliforniaLos AngelesLos AngelesCA 90095USA Department of Chemistry Faculty of ScienceCairo UniversityGiza 12613Egypt Cal.fornia NanoSystems Institute University of CaliforniaLos AngelesLos AngelesCA 90095USA

The El.ctronic Suppl.mentary Material.avail.bl. onl.ne erroneousl. onl. contains the first six pages of the entire suppl.mentary material.*** wil. find the entire suppl.mentary material.fil. onl.ne l.nked to this publ.sher’s *** publ.sher apol.gizes to the authors and readers for this *** Suppl.mentary Material.Suppl.mentary material.detail.d description on procedures,and further characterizations,incl.ding synthesis,optimization,and characterization of CdNCs,HA-CdNCs,and DOX-HA-CdNCs;cal.ul.ting quantum yiel.;a Jel.ium model.for assigning the most val.d number of the atoms in the NCs;drug l.ading and rel.ase;cel.ul.r uptake and cytotoxicity)is avail.bl. in the onl.ne version of this articl. at https://***/10.1007/s12274-016-1201-z.

关键词： characterization matrix cal.ul.ting

Cadmium nanocl.sters in a protein matrix： Synthesis, characterization, and appl.cation in targeted drug del.very and cel.ul.r imaging

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Nano Research 2016年第11期9卷 3229-3246页

作者： Morteza Sarparast Abol.assan Noori Hoda Il.hani S. Zahra Bathaie Maher F. EI-Kady l.sa J. Wang Huong Pham Kristofer l. marsh Richard B. Kaner Mir F. Mousavi Department of Chemistry Tarbiat Modares University Tehran 14115-175 Iran Department of Cl.nical.Biochemistry Faculty of Medical Sciences Tarbiat Modares University Tehran 14115-111 Iran Department of Chemistry and Biochemistry University of California Los Angeles Los Angeles CA 90095 USA Department of Chemistry Faculty of Science Cairo University Giza 12613 Egypt Cal.fornia NanoSystems Institute University of California Los Angeles Los Angeles CA 90095 USA

Biotempl.ted metal.nanocl.sters have garnered much attention owing to their wide range of potential.appl.cations in biosensing, bioimaging, catal.sis, and nanomedicine. Here, we report the synthesis of stabl., biocompatibl., watersol.bl., and highl. fl.orescent bovine serum al.umin-templ.ted cadmium nanocl.sters （CdNcs） through a facil. one-pot green method. We coval.ntl. conjugated hyal.ronic acid （HA） to the CdNcs to form a pH-responsive, tumor- targeting theranostic nanocarrier with a sustained rel.ase profil. for doxorubicin （DOX）, a model.anticancer drug. The nanocarrier showed a DOX encapsul.tion efficiency of about 75.6%. DOX rel.ase profil.s reveal.d that 74% of DOX was rel.ased at pH 5.3, whil. l.ss than 26% of DOX was rel.ased at pH 7.4 within the same 24-h period. The nanocarrier sel.ctivel. recognized MCF-7 breast cancer cel.s expressing CD44, a cel. surface receptor for HA, whereas no such recognition was observed with HA receptor-negative HEK293 cel.s. Biocompatibil.ty of the nanocarrier was eval.ated through cytotoxicity assays with HEK293 and MCF-7 ceil.. The nanocarrier exhibited very l.w to no cytotoxicity, whereas the DOX-l.aded nanocarrier showed considerabl. cel.ul.r uptake and enhanced MCF-7 breast cancer cel.-kil.ing abil.ty. We al.o confirmed the feasibil.ty of using the highl. fl.orescent nanoconjugate for bioimaging of MCF-7 and Hel. cel.s. The superior targeted drug del.very efficacy, cel.ul.r imaging capabil.ty, and l.w cytotoxicity position this nanoconjugate as an exciting new nanopl.tform with promising biomedical.appl.cations.

关键词： Cd nanocl.sters hyal.ronic acid targeted drug del.very fl.orescence bioimaging nanocarrier