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Detection and evaluation of different morphological forms of mycoplasma hominis in human semen

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Asian Journal of Andrology 2024年第1期26卷 71-76页

作者： Elizaveta Bragina tatyana sorokina Avtandil Chogovadze Yulia Shevchenko Vyacheslav Chernykh Olga Barkhatova Irina Rakovskaya Research Centre for medical Genetics Moscow 115522Russia A.N.Belozersky Institute of Physico-Chemical Biology Lomonosov moscow State University Moscow 119992Russia Reprolab Limited Liability Company Moscow 119333Russia N.I.Pirogov Russian National Research medical University Moscow 117997Russia Federal Government Budgetary Institution"National Research Center for Epidemiology and microbiology Named after Honorary Academician N.F.Gamaleya of the ministry of Health of the Russian Federation" Moscow 123098Russia

Recently discovered microcolonial forms of mycoplasma hominis(***)and their impact on human spermatogenesis are *** spermatozoa of 125 fertile men(sperm donors;from Reprobank[Reproductive Tissue Bank,moscow,Russial)and of 93 patients with fertility problems(from the Federal State Budgetary Institution"Research Centre for medical Genetics[RCmG]",moscow,Russia)were *** colonies of *** and microcolonies were detected by molecular biological methods,culture of bacteria,and transmission electron *** unique structure of microcolonial cells,in which the cytoplasmic cylinder is surrounded by concentric electron-dense and electron-light layers with a periodicity of 12-14 nm,and the ability of microcolonial cells to attach to spermatozoa are *** patients with lower sperm quality,microcolonies of *** were detected 2.5 times more frequently than classical *** detection of microcolonies in the ejaculate and the frequent isolation of microcolonies from sperm samples of patients with fertility problems suggest that microcolonial cells may be one cause of infertility.

关键词： ejaculate fertility mycoplasma hominis spermatogenesis transmission electron microscopy

Weathering reflected by the chemical composition of alluvial soils from the Zeya and Selemdzha river valleys

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Science China Earth Sciences 2018年第5期61卷 604-613页

作者： O.A.sorokina m.N.GYSEV Institute of Geology and Nature management Far East Branch Russian Academy of Sciences

The paper examines the chemical composition of alluvial soils from the Zeya and Selemdzha River valleys, one of the branch rivers in the Amur River basin. It has been established that the compositions of these soils are characterized by a relative shortage of practically all analyzed macro-and microelements as compared with UCC(upper continental crust) and PAAS(postArhean Australian average shale) compositions. The chemical composition of alluvial soils approximates that of the soils in NE China and Korea due to similar climatic and geographic conditions. On the basis of relationships between major and trace elements and peculiarities of trace elements concentration it is shown that the alluvial soils in the middle reaches of the Zeya River were formed by the sources largely transformed by chemical weathering processes. By comparison, the soils in the Selemdzha River and lower reaches of the Zeya River(below the Selemdzha River mouth) are dominated by material to a large degree reworked by the physical processes rather than chemical ones. This inference conforms with specific geological settings of the water-collector.

关键词： Alluvial soils Weathering indices Amur Zeya

CRITICAL ISSUES IN THE NUmERICAL TREATmENT OF THE PARAmETER ESTImATION PROBLEmS IN ImmUNOLOGY

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Journal of Computational mathematics 2012年第1期30卷 59-79页

作者： tatyana Luzyanina Gennady Bocharov Institute of mathematical Problems in Biology RAS Pushchino Moscow reg. Russia Institute of Numerical mathematics RAS Moscow Russia

A robust and reliable parameter estimation is a critical issue for modeling in immunology. We developed a computational methodology for analysis of the best-fit parameter estimates and the information-theoretic assessment of the mathematical models formulated with ODEs. The core element of the methodology is a robust evaluation of the first and second derivatives of the model solution with respect to the model parameter values. The critical issue of the reliable estimation of the derivatives was addressed in the context of inverse problems arising in mathematical immunology. To evaluate the first and second derivatives of the ODE solution with respect to parameters, we implemented the variational equations-, automatic differentiation and complex-step derivative approximation methods. A comprehensive analysis of these approaches to the derivative approximations is presented to understand their advantages and limitations.

关键词： mathematical modeling in immunology Parameter estimation Constrainedoptimization.

Irrigation erosion of irrigated soils in the foothills of southern Kazakhstan

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Journal of Arid Land 2013年第2期5卷 166-171页

作者： Abdulla S SAPAROV Enadiy K МIRZAKEEV tatyana m SHARYPOVA Galymzhan A SAPAROV Jilili ABUDUWAILI Kazakh Research Institute of Soil Science and Agrochemistry named after Uspanov Almaty 050060 Kazakhstan State Key Laboratory of Desert and Oasis Ecology Xinjiang Institute of Ecology and Geography Chinese Academy of SciencesUrumqi 830011 China

Abstract： This paper presented the results from the study of irrigation erosion of irrigated lands in southern Ka- zakhstan. The main purpose of the work is the conservation of the fertility of irrigated soils, and then the soils of the upper, middle and lower sections of the slopes at the experimental site were studied. Based on field investigations, authors studied the water resistance of aggregates of gray-brown soils and light gray soils before and after irrigation and qualitative indicators of changes in physical and chemical properties of structured soils in irrigation were de- termined by conventional methods of soil research. The results indicated that the changes in the physical properties of soils by using polymeric compounds created a fundamentally new opportunity to control water erosion of soils.

关键词： irrigation erosion furrow irrigation structure-forming polymers deformation of furrows permissible water speed aggregate water stability

GIS tools for correlation of tectonics and seismicity in the Altay-Sayan area,Russia

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Geoscience Frontiers 2010年第1期1卷 133-141页

作者： Raissa m.Lobatskaya tatyana G.Krasnoramenskaya Irkutsk State Technological University Irkutsk 664074Russia Center for Environment and Resources Conservation Krasnoyarsk 660049Russia

Fault-block structures of the Altay-Sayan folded area （ASFA） southeastern Siberia of Russia were used as the basis for creating a 3-D model. The surface structures were projected to depths by previous correlations between long and deep faults, with all layers and deformation factors defined. The mean deformation factor （Ds） is 0.12 unit/km^3 in the upper layer, 0.012 unit/km^3 in the intermediate layer, and 0.007 unit/km^3 in the lower layer of the 3-D ASFA neotectonic model. Ds allows correlation of the three distinguished layers with theological bodies that differ in their potential for accumulating elastic energy. 3-D modeling can be used as a methodological approach to projections in seismic prone areas such as the Krasnoyarsk region, for earthquake-hazard monitoring.

关键词： Fault-block structures 3-D modeling GIS methods Earthquake-hazard Altay-Sayan folded area Russia

Pharmacological and Predicted Activities of Natural Azo Compounds

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Natural Products and Bioprospecting 2017年第1期7卷 151-169页

作者： Valery m.Dembitsky tatyana A.Gloriozova Vladimir V.Poroikov National Scientific Center of marine Biology 17 Palchevsky Str.VladivostokRussia 690041 Institute of Biomedical Chemistry MoscowRussia 119121

This paper describes research on natural azo compounds isolated from fungi,plant,bacteria,and *** than 120 biologically active diazene containing alkaloids demonstrate confirmed pharmacological activity,including antitumor,antimicrobial,and antibacterial *** structures,origin,and biological activities of azo compounds are *** the computer program PASS,some structure–activity relationship new activities are also predicted,pointing toward possible new applications of these *** article emphasizes the role of natural azo compounds as an important source of drug prototypes and leads for drug discovery.

关键词： Azo metabolites Alkaloids Fungi Plant Bacteria Sponges SAR

Benefits of Ilizarov automated bone distraction for nerves and articular cartilage in experimental leg lengthening

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World Journal of Orthopedics 2017年第9期8卷 688-696页

作者： Nathalia Shchudlo tatyana Varsegova tatyana Stupina michael Shchudlo marat Saifutdinov Andrey Yemanov Laboratory of morphology FSBI Russian Ilizarov Scientific Center "Restorative Traumatology and Orthopaedics"

AIm To determine peculiarities of tissue responses to manual and automated Ilizarov bone distraction in nerves and articular *** Twenty-nine dogs were divided in two experimental groups: Group m-leg lengthening with manual distraction(1 mm/d in 4 steps), Group A-automated distraction(1 mm/d in 60 steps) and intact group. Animals were euthanized at the end of distraction, at 30 th day of fixation in apparatus and 30 d after the fixator removal. m-responses in gastrocnemius and tibialis anterior muscles were recorded, numerical histology of peronealand tibialis nerves and knee cartilage semi-thin sections, scanning electron microscopy and X-ray electron probe microanalysis were *** Better restoration of m-response amplitudes in leg muscles was noted in A-group. Fibrosis of epineurium with adipocytes loss in peroneal nerve, subperineurial edema and fibrosis of endoneurium in some fascicles of both nerves were noted only in m-group, shares of nerve fibers with atrophic and degenerative changes were bigger in m-group than in A-group. At the end of experiment morphometric parameters of nerve fibers in peroneal nerve were comparable with intact nerve only in A-group. Quantitative parameters of articular cartilage(thickness, volumetric densities of chondrocytes, percentages of isogenic clusters and empty cellular lacunas, contents of sulfur and calcium) were badly changed in m-group and less changed in *** Automated Ilizarov distraction is more safe method of orthopedic leg lengthening than manual distraction in points of nervous fibers survival and articular cartilage arthrotic changes.

关键词： Limb lengthening Articular cartilage Nerve Histomorphometry Dog

monitoring cellular metabolism of 3T3 upon wild type E. coli infection by mapping NADH with FLIm

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Chinese Optics Letters 2010年第10期8卷 931-933页

作者： tatyana Buryakina Pin-Tzu Su Vladimir Gukassyan Wan-Jr Syu Fu-Jen Kao Institute of Biophotonics National Yang-Ming University Institute of microbiology and Immunology National Yang-Ming University Department of Photonics National Sun Yat-Sen University

Fluorescence lifetime imaging microscopy （FLIm） has gained popularity as a sensitive technique to monitor the functional/conformational states of reduced nicotinamide adenine dinucleotide （NADH）, one of the main compounds of oxidative phosphorylation. In this letter, we apply the technique to characterize the metabolic changes in mouse embryonic fibroblast 3T3 cells upon bacterial infection. A gradual shortening of the decaying time constants in both the short and the long lifetime components of NADH＇s autofluorescence is detected. The ratio of the short and the long lifetime components＇ relative contributions, however, shows a rapid increase, indicating the rise of cellular metabolic activity over the course of infection.

关键词： Amides Cell culture Phosphorylation Tissue

Assessment of palmar subcutaneous tissue vascularization in patients with Dupuytren's contracture

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World Journal of Orthopedics 2018年第9期9卷 130-137页

作者： Nathalia Shchudlo tatyana Varsegova tatyana Stupina Tamara Dolganova michael Shchudlo Nathalia Shihaleva Vadim Kostin Clinics and Experimental Laboratory for Reconstructive microsurgery and Hand Surgery Russian Ilizarov Scientific Center "Restorative Traumatology and Orthopaedics" Laboratory of morphology of Federal State Budget Institution Russian Ilizarov Scientific Center "Restorative Traumatology and Orthopaedics"

AIm To investigate the structural and functional characte-ristics of palmar hypodermal tissue vascularization in Dupuytren's contracture patients of different *** Eighty-seven Dupuytren's contracture patients underwent partial fasciectomy. Twenty-two of them were less than 55 years old(Y-group, n = 22); the others were 55 and older(O-group, n = 65). In surgically excised representative tissue samples, a histomorphometric analysis of the perforating arteries of the palmar aponeurosis and stereologic analysis of hypodermis vascularity were performed. The method of laser flowmetry estimated the microcirculation of the skin of the *** Frequency of cases with rapid development of contracture(less than 5 years) was 13.6% in the Y-group and 40% in the O-group, P minal diameters of perforating arteries in palmar fascia were decreased more severely in Y. The thickness of intima increased three times compared with healthy control, and the intima/media relation also increased, especially in O. Increased numerical and volumetric micro-vessel densities in hypodermis, percentage of large vessels(more than 12 μm in diameter), and percentage of vessels with signs of periadventitial inflammatory infiltration were noted in Y. The percentage of vessels with adventitial fibrosis was greater in O than in Y. Base capillary flow in Y was increased compared to healthy control subjects and to O, and peak capillary flow was increased in comparison with *** Compared to the O-group, Y-group patients exhibited more severe constrictive remodeling of palmar fascia perforating arteries supplying hypodermis but more expressed compensatory changes of its capillarization.

关键词： Dupuytren’s contracture Laser Doppler flowmetry Hypodermis Histo-morphometry Palmar fascia