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Evolutionary Mode of Metallothioneins Inferred from Cd-MT Genes of Tetrahymena

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Wuhan University Journal of Natural Sciences 2005年第5期10卷 925-930页

作者： WAN ming-liang FENG Wei-song MIAO Wei wong ming-hong State Key Laboratory of Freshwater Ecology and Biotechnology Institute of Hydrobiology Chinese Academyof Sciences Wuhan 430072 Hubei China Institute for Natural Resources and Environmental Management Hong Kong Baptist University Kowloon Tong Hong Kong China

From Tetrahymena thermophila （strain BF5）, the coding region of Cd-MT gene was cloned and sequenced. and identified as MTT1 isoform. A serial duplicate structure is discovered in its amino acid sequence, which separates the coding region into three parts （Part 1：7-61; Part 2：64-118; Part 3：122-162）. The alignments among them and comparison with the corresponding parts of MT1 isoform suggest that MT1 and MTT1 isoforms both come from the same ancient gene that is homologous to Part 1, and Cd-MTs of Tetrahymena are aroused by such ancient gene duplication. The prediction of secondary structures and the analysis of the disulfide-bonding state of cysteine show that there are a lot of differences between MT1 and MTT1 isoforms, which maybe relate to their function mechanism.

关键词： Tetrahymena tkermopkila metallothioneins evolutionary mode gene duplication protein spatial structure metal-binding sites

Influence of screw length and diameter on tibial strain energy density distribution after anterior cruciate ligament reconstruction

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Acta Mechanica Sinica 2014年第2期30卷 241-249页

作者： Jie Yao Guan-ming Kuang Duo Wai-Chi wong Wen-Xin Niu ming Zhang Yu-Bo Fan Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education School of Biological Science and Medical Engineering/National Key Lab of Virtual Reality TechnologyBeihang University Interdisciplinary Division of Biomedical Engineering Faculty of EngineeringThe Hong Kong Polytechnic University Shenzhen Key Laboratory for Innovative Technology in Orthopaedic Trauma Department of Orthopaedics and TraumatologyThe University of Hong Kong-Shenzhen Hospital The University of Hong Kong Tongji University School of Medicine

Postoperative tunnel enlargement has been frequently reported after anterior cruciate ligament（ACL）*** screw,as a surgical implant in ACL reconstruction,may influence natural loading transmission and contribute to tunnel *** aims of this study are（1）to quantify the alteration of strain energy density（SED）distribution after the anatomic single-bundle ACL reconstruction;and（2）to characterize the influence of screw length and diameter on the degree of the SED alteration.A validated finite element model of human knee joint was *** screw length ranging from 20 to 30 mm with screw diameter ranging from 7 to 9 mm were *** the post-operative knee,the SED increased steeply at the extra-articular tunnel aperture under compressive and complex loadings,whereas the SED decreased beneath the screw shaft and nearby the intra-articular tunnel *** the screw length could lower the SED deprivation in the proximal part of the bone tunnel;whereas increasing either screw length or diameter could aggravate the SED deprivation in the distal part of the bone *** the elastic modulus of the screw could lower the bone SED deprivation around the *** consideration of both graft stability and SED alteration,a biodegradable interference screw with a long length is recommended,which could provide a beneficial mechanical environment at the distal part of the tunnel,and meanwhile decrease the bone-graft motion and synovial fluid propagation at the proximal part of the *** findings together with the clinical and histological factors could help to improve surgical outcome,and serve as a preliminary knowledge for the following study of biodegradable interference screw.

关键词： Anterior crucial ligament reconstruction Strain energy density Screw length Screw diameter

Tuna dark muscle peptides modulate host iron metabolism in the intestine through the gut microbiota and ameliorate iron deficiency anemia

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Food Science and Human Wellness 2024年

作者： Jiaojiao Han Jiayi Xiong Zhixiang Bai Yang Qin Chenyang Lu Jun Zhou Ye Li Tinghong ming Zhen Zhang Xiurong Su State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products， Ningbo University School of Marine Science， Ningbo University

Iron deficiency anemia (IDA) is a nutritional deficiency disease with a high incidence rate worldwide. Bioactive peptides are safe and effective， have multiple functions and can serve as potential candidates for alleviating IDA. In this study， the anti-anemia effects of tuna dark muscle peptides were explored in a diet-induced IDA mouse model. The results showed that tuna dark muscle peptides alleviated the IDA phenotype， oxidative stress and iron metabolism. In addition， tuna dark muscle peptides reversed gut microbiota dysbiosis in IDA mice. Furthermore， the transplanted fecal microbiota from tuna dark muscle peptide-treated mice also alleviated IDA symptoms and regulated iron metabolism and the gut microbiota， indicating that the anti-anemic effects were at least partially mediated by the gut microbiota. Thus， we identified a new and safe prebiotic material to alleviate IDA and provided ideas for the development of peptides. At the same time， these data also provided a theoretical basis for fecal microbiota transplantation (FMT) to alleviate IDA.

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An enamel-inspired bioactive material with multiscale structure and antibacterial adhesion property

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Bioactive Materials 2022年第1期7卷 491-503页

作者： Hai ming wong Yu Yuan Zhang Quan Li Li Paediatric Dentistry and Orthodontics Faculty of DentistryThe University of Hong Kong34 Hospital RoadHong Kong Collage and Hospital of Stomatology Anhui Medical UniversityNo.69Meishan RoadHeifeiChina

Conventional dental materials lack of the hierarchical architecture of enamel that exhibits excellent intrinsic-extrinsic mechanical ***,restorative failures frequently occur due to physical and chemical mismatch between artificial materials and native dental hard tissue followed by recurrent caries which is caused by sugar-fermenting,acidogenic bacteria invasion of the defective *** order to resolve the limitations of the conventional dental materials,the aim of this study was to establish a non-cell-based biomimetic strategy to fabricate a novel bioactive material with enamel-like structure and antibacterial adhesion *** evaporation-based,bottom-up and self-assembly method with layer-by-layer technique were used to form a large-area fluorapatite crystal layer containing antibacterial *** multilayered structure was constructed by hydrothermal growth of the fluorapatite crystal layer and highly conformal adsorption to the crystal surface of a polyelectrolyte matrix *** and mechanical assessment demonstrated that the synthesized bioactive material resembled the native enamel in chemical components,mechanical properties and crystallographic *** and cytocompatibility evaluation demonstrated that this material had the antibacterial adhesion property and *** combination with the molecular dynamics simulations to reveal the effects of variables on the crystallization mechanism,this study brings new prospects for the synthesis of enamel-inspired materials.

关键词： Biomimetic mineralization Enamel-inspired material Bioactive material Graphene oxide Enamel-like structure

Screening and early detection of pancreatic cancer in high risk population

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World Journal of Gastroenterology 2014年第9期20卷 2358-2364页

作者： ming-Chu Chang Jau-Min wong Yu-Ting Chang Department of Internal Medicine National Taiwan University Hospital College of Medicine National Taiwan University

Pancreatic cancer is a serious growing health issue in developed *** patients diagnosed with pancreatic cancer,the five year survival rate is below 5%.One major important reason leads to the poor survival rate is lack of early detection of pancreatic *** 80% of the patients are diagnosed in advanced disease *** for pancreatic cancer is a desirable option for high risk individuals to allow early detection and treatment of curable pancreatic neoplasms at a pre-invasive *** article highlights the need,endpoint,population,method,diagnostic yield,and the problems of current screening programs.

关键词： Pancreatic cancer screening High risk population Pancreatic neoplasm Peutz-Jeghers syndrome

Curriculum Adaptation in Special Schools for Students with Intellectual Disabilities(SID):A Case Study of Project Learning in One SID School in Hong Kong

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Frontiers of Education in China 2014年第2期9卷 250-273页

作者： ZHANG Jia-Wei wong Lam CHAN tak-Hang CHIU Chi-Shing Hong Kong Institute of Educational Research The Chinese University of Hong KongHong KongChina

Using a qualitative case study approach,the authors analyzed the curriculum adaptation process for one project learning activity in School K,which is a SID school in the context of school-university *** sources of data were collected for triangulation,including interviews,documents and *** adaptation strategies in this study were analyzed from five perspectives:instructional goals,instructional content,instructional strategies,instructional settings,and student behavioral *** was found that curriculum adaptation efforts could help students with ID develop potential at their own level through project learning activates and teachers could also gain professional development during the university-school collaboration *** for future studies,enlarging the sample size,involving teachers’past orientations and motivations in the project learning process,and collecting quantitative data could all be taken into account.

关键词： special education students with intellectual disabilities(SID) curriculum adaptation project learning

Recent Developments of Genomic Research in Soybean

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Journal of Genetics and Genomics 2012年第7期39卷 317-324页

作者： Ching Chan Man-Wah Li Fuk-Ling wong Hon-ming Lam State Key Laboratory of Agrobiolechnology and School of Life Sciences The Chinese University of Hong Kong

Soybean is an important cash crop with unique and important traits such as the high seed protein and oil contents, and the ability to perform symbiotic nitrogen fixation. A reference genome of cultivated soybeans was established in 2010, followed by whole-genome re-sequencing of wild and cultivated soybean accessions. These efforts revealed unique features of the soybean genome and helped to understand its evolution. Mapping of variations between wild and cultivated soybean genomes were perfo^aned. These genomic variations may be related to the process of domestication and human selection. Wild soybean germplasms exhibited hiigh genomic diversity and hence may be an important source of novel genes/alleles. Accumulation of genomic data will help to refine genetic maps and expedite the identification of functional genes. In this review, we summarize the major findings from the whole-genome sequencing projects and discuss the possible impacts on soybean researches and breeding programs. Some emerging areas such as transcriptomic and epigenomic studies will be introduced. In addition, we also tabulated some useful bioinformatics tools that will help the mining of the soybean genomic data.

关键词： Evolution Genome Soybean

Crocodile blood supplementation protect svascular function in diabetic mice

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Food Production, Processing and Nutrition 2021年第1期3卷 283-295页

作者： Chui Yiu Bamboo Chook Francis M.Chen Gary Tse Fung Ping Leung Wing tak wong School of Life Sciences Faculty of ScienceThe Chinese University of Hong KongShatinNTHong Kong SAR State Key Laboratory of Agrobiotechnology The Chinese University of Hong KongHong KongChina

Cardiovascular disease is a major cause of mortality in diabetic patients due to the heightened oxidative stress and pro-inflammatory state in vascular *** approaches targeting cardiovascular health for diabetic patients are urgently *** blood,an emerging dietary supplement,was suggested to have anti-oxidative and anti-inflammatory effects in vitro,which have yet to be proven in animal *** study thereby aimed to evaluate whether crocodile blood can protect vascular function in diabetic mice against oxidation and *** db/db mice and their counterparts db/m+mice were treated daily with crocodile blood soluble fraction(CBSF)or vehicle via oral gavage for 4 weeks before their aortae were harvested for endothelium-dependent relaxation(EDR)quantification using wire myograph,which is a well-established functional study for vascular function *** culture experiments culturing mouse aortae from C57BL/6 J mice with or without IL-1βand CBSF were done to evaluate the direct effect of CBSF on endothelial *** oxygen species(ROS)levels in mouse aortae were assessed by dihydroethidium(DHE)staining with inflammatory markers in endothelial cells quantified by quantitative polymerase chain reaction(qPCR).CBSF significantly improved deteriorated EDR in db/db diabetic mice through both diet supplementation and direct culture,with suppression of ROS level in mouse *** also maintained EDR and reduced ROS levels in mouse aortae against the presence of pro-inflammatory IL-1β.Under the pro-inflammatory state induced by IL-1β,gene expressions of inflammatory cytokines were downregulated,while the protective transcripts UCP2 and SIRT6 were upregulated in endothelial *** study suggests a novel beneficial effect of crocodile blood on vascular function in diabetic mice and that supplementation of diet with crocodile blood may act as a complementary approach to protect against vascular diseases through anti

关键词： Crocodile blood Diabetes mellitus Vascular endothelial function Anti-oxidative Anti-inflammatory

Distribution patterns of octylphenol and nonylphenol in the aquatic system at Mai Po Marshes Nature Reserve,a subtropical estuarine wetland in Hong Kong

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Journal of Environmental Sciences 2007年第6期19卷 657-662页

作者： LI Xiang-li LUAN Tian-gang LIANG Yan wong ming-hung LAN Chong-yu Croucher Institute for Environmental Sciences and Department of Biology Hong Kong Baptist UniversityHong Kong China State Key Laboratory of Biocontrol School of life Sciences Sun Yat-Sen （Zhongshan） University Guangzhou 510275 China

Spatial and temporal distribution of octylphenol （OP） and nonylphenol （NP） in Mai Po Marshes, a subtropical estuarine wetland in Hong Kong, were investigated. Surface water samples were collected every month from 11 sites during the period of September- December 2004. Concentrations of OP and NP ranged from 11.3 to 348 ng/L and from 29 to 2591 ng/L, respectively. The high levels of NP and OP were found in November and December than in September and October. The levels of OP and NP have no significant spatial differences except September. Total organic matter in the sediments appeared to be an important factor in controlling the fate of these compounds in the aquatic environment.

关键词： octylphenol nonylphenol endocrine-disrupting chemicals Ramsar Site