Evolutionary Mode of Metallothioneins Inferred from Cd-MT Genes of Tetrahymena

作     者：WAN Ming-liang FENG Wei-song MIAO Wei WONG Ming-hong 

作者机构：State Key Laboratory of Freshwater Ecology and Biotechnology Institute of Hydrobiology Chinese Academyof Sciences Wuhan 430072 Hubei China Institute for Natural Resources and Environmental Management Hong Kong Baptist University Kowloon Tong Hong Kong China 

出 版 物：《Wuhan University Journal of Natural Sciences》 (武汉大学学报（自然科学英文版）)

年 卷 期：2005年第10卷第5期

页      面：925-930页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071002[理学-动物学] 

基　　金：SupportedbyKSCXofChineseAcademyofSciencesGrant(SW102)andtheAllocationfromtheEarmaarkedGrantforHongKong 

主　　题：Tetrahymena tkermopkila metallothioneins evolutionary mode gene duplication protein spatial structure metal-binding sites 

摘      要：From Tetrahymena thermophila （strain BF5）, the coding region of Cd-MT gene was cloned and sequenced. and identified as MTT1 isoform. A serial duplicate structure is discovered in its amino acid sequence, which separates the coding region into three parts （Part 1：7-61; Part 2：64-118; Part 3：122-162）. The alignments among them and comparison with the corresponding parts of MT1 isoform suggest that MT1 and MTT1 isoforms both come from the same ancient gene that is homologous to Part 1, and Cd-MTs of Tetrahymena are aroused by such ancient gene duplication. The prediction of secondary structures and the analysis of the disulfide-bonding state of cysteine show that there are a lot of differences between MT1 and MTT1 isoforms, which maybe relate to their function mechanism.