检索结果-南通市图书馆

Duplex micro RNAs assay based on target-triggered universal reporter hybridization

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Pharmaceutical Analysis 2018年第4期8卷 265-270页

作者： Yinan Wang Yue Sun ch.iwan lau Jianzhong Lu Sch.ol of Pharmacy Fudan University

In this paper, we designed and evaluated a duplex detection strategy for micro RNAs（mi RNAs） using universal probe-based target-triggered double hybridization and fluorescent microsphere-based assay system（x MAP array）. In the absence of target mi RNA, reporter DNA cannot hybridize stably with the immobilized capture DNA due to its low melting temperature. Only after adding target mi RNA, can reporter probe hybridize with capture probe to form a stable three-component complex. This targettriggered stable hybridization makes this method possible for highly selective and sensitive detection of multiple mi RNAs. We exemplified a quantitative detection of duplex mi RNAs with a limit of detection of40 p M. The x MAP array platform holds the potential of extending this approach.to simultaneous detection of up to 100 mi RNA targets. Considering the simplicity, rapidity and multiplexing, this work promised a potential detection of multiple mi RNA biomarkers for early disease diagnosis and prognosis.

关键词： xMAP array Multiple microRNAs Target-triggered Universal reporter

Deterministic approach.s for noncoherent communicationswith ch.otic carriers

在线全文

学校读者我要写书评

暂无评论

Journal of Systems Engineering and Electronics 2005年第2期16卷 253-257页

作者： LiuXiongying QiuShuisheng Francis.C.M.lau CollegeofElectronicandInformationEngineering SouthChinaUniversityofTechnologyGuangzhou510640P.R.China DepartmentofElectronicandInformationEngineering HongkongPolytechnicUniversityHongkongP.R.China

Two problems are proposed. The first one is the noise decontamination of ch.otic carriers using a deterministic approach.to reconstruct pseudo trajectories, the second is the design of communications sch.mes with ch.otic carriers. After presenting our deterministic noise decontamination algorithm, conventional ch.os shift keying (CSK) communication system is applied. The difference of Euclidean distance between noisy trajectory and decontaminated trajectory in phase space could be utilized to non-coherently detect the sent symbol simply and effectively. It is shown that this detection method can ach.eve the bit error rate performance comparable to other non-coherent systems.

关键词： gradient descent reconstruction ch.otic communications noncoherent detection.

Wan-Yee Joseph lau:the promoter of modernization and internationalization of ch.nese surgery

在线全文

学校读者我要写书评

暂无评论

Oncology and Translational Medicine 2024年第1期10卷 1-3页

作者： Wan-Yee Joseph lau Faculty of Medicine The Chinese University of Hong KongHong KongChina

To my knowledge,there have been 3 books[1–3]and 2 book ch.pters written about me.[4–5]For those who are interested in the details of my life,please refer to these books/book *** title of this article is an English translation of the title of the book I refer to as reference 1,which.I find appropriate to describe the role that I play in ch.na.

关键词： please details translation

Cosmic microwave background constraints on the tensor-to-scalar ratio

在线全文

学校读者我要写书评

暂无评论

Research.in Astronomy and Astrophysics 2014年第6期14卷 635-647页

作者： King lau Jia-Yu Tang Ming-ch.ng ch. Department of Physics and Institute of Theoretical Physics The Chinese University of Hong Kong

One of the main goals of modern cosmic microwave background （CMB） missions is to measure the tensor-to-scalar ratio r accurately to constrain inflation models. Due to ignorance about the reionization history Xe （z）, this analysis is usu- ally done by assuming an instantaneous reionization Xe （z） which. however, can bias the best-fit value of r. Moreover, due to the strong mixing of B-mode and E-mode polarizations in cut-sky measurements, multiplying the sky coverage fraction fsky by the full-sky likelihood would not give satisfactory results. In this work, we forecast constraints on r for the Planck mission taking into account the general reionization scenario and cut-sky effects. Our results show that by applying an N-point interpo- lation analysis to the reionization history, the bias induced by the assumption of in- stantaneous reionization is removed and the value of r is constrained within 5% error level, if the true value of r is greater than about 0.1.

关键词： cosmology: cosmic microwave background -- cosmology: cosmologicalparameters -- cosmology: early universe -- gravitational waves

Primary Immunodeficiencies:“New” Disease in an Old Country

在线全文

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2009年第6期6卷 397-406页

作者： Pamela P.W.Lee Yu-Lung lau Department of Paediatrics and Adolescent Medicine The University of Hong KongHKSARChina Department of Paediatrics and Adolescent Medicine Room 1171/F New Clinical BuildingQueen Mary HospitalPokfulamHong KongChina

Primary immunodeficiency disorders(PIDs)are rare inborn errors of the immune *** with PIDs are unique models that exemplify the functional and phenotypic consequences of various immune defects underlying infections,autoimmunity,lymphoproliferation,allergy and *** 150 PID syndromes were ch.racterized in the past 60 years,with an ever growing list of new entities being *** of their rarity,multi-center collaboration for pooled data analysis and molecular studies is important to gain meaningful insights into the phenotypic and genetic diversities of *** this article,we summarize our research.findings on PIDs in ch.nese population in the past 20 *** collaboration among various immunology centers,cross-referrals and systematic data analysis constitute the foundation for research.on *** directions include establishment of a national PID registry,raising awareness of PIDs and securing sufficient resources for patient care and scientific research.

关键词： primary immunodeficiency disorders immunodeficiencies development research ch.na

Modeling of cable vibration effects of cable-stayed bridges

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Earthquake Engineering and Engineering Vibration 2002年第1期1卷 74-85页

作者： S.H.ch.ng David T.lau Department of Civil and Environmental Engineering Carleton UniversityOttawaOntarioCanadaPost-doctoral fellow Department of Civil and Environmental Engineering Carleton UniversityOttawaOntarioCanada Professor

The analysis of dynamic responses of cable-stayed bridges subjected to wind and earthquake loads generally considers only the motions of the bridge deck and *** influence of the stay cable vibration on the responses of the bridge is either ignored or considered by approximate *** transverse vibration of the stay cables,which.can be significant in some cases,are usually neglected in previous *** the present study,a new three-node cable element has been developed to model the transverse motions of the *** interactions between the cable behavior and the other parts of the bridge superstructure are considered by the concept of dynamic *** nonlinear effect of the cable caused by its self-weight is included in the *** examples are presented to demonstrate the accuracy and efficiency of the proposed model. The impact of cable vibration behavior on the dynamic ch.racteristics of cable-stayed bridges is discussed.

关键词： cable-stayed bridges cable vibration dynamics finite elements long-span structures

Battle of the sexes: contrasting roles of testis-specific protein Y-encoded (TSPY) and TSPX in human oncogenesis

在线全文

学校读者我要写书评

暂无评论

Asian Journal of Andrology 2019年第3期21卷 260-269页

作者： Yun-Fai ch.is lau Yunmin Li Tatsuo Kido Division of Cell and Developmental Genetics Department of Medicine VA Medical Center and Institute for Human Genetics University of California San Francisco CA 94121 USA

The Y-located testis-specific protein Y-encoded (TSPY) and its X-homologue TSPX originated from the same ancestral gene, but act as a proto-oncogene and a tumor suppressor gene, respectively. TSPY has specialized in male-specific functions, while TSPX has assumed the functions of the ancestral gene. Both TSPY and TSPX harbor a conserved SET/NAP domain, but are divergent at flanking structures. Specifically, TSPX contains a C-terminal acidic domain, absent in TSPY. They possess contrasting properties, in which.TSPY and TSPX, respectively, accelerate and arrest cell proliferation, stimulate and inhibit cyclin B-CDK1 phosphorylation activities, have no effect and promote proteosomal degradation of the viral HBx oncoprotein, and exacerbate and repress androgen receptor (AR) and constitutively active AR variant, such.as AR-V7, gene transactivation. The inhibitory domain has been mapped to the carboxyl acidic domain in TSPX, truncation of which.results in an abbreviated TSPX exerting positive actions as TSPY. Transposition of the acidic domain to the C-terminus of TSPY results in an inhibitory protein as intact TSPX. Hence, genomic mutations/aberrant splicing events could generate TSPX proteins with truncated acidic domain and oncogenic properties as those for TSPY. Further, TSPY is upregulated by AR and AR-V7 in ligand-dependent and ligand-independent manners, respectively, suggesting the existence of a positive feedback loop between a Y-located proto-oncogene and male sex hormone/receptors, thereby amplifying the respective male oncogenic actions in human cancers and diseases. TSPX counteracts such.positive feedback loop. Hence, TSPY and TSPX are homologues on the sex ch.omosomes that function at the two extremes of the human oncogenic spectrum.

关键词： androgen receptor AR-V7 cell cycle regulation cyclin B-CDK1 oncogene sex ch.omosomes sex differences TSPX TSPY tumor suppressor

Line-of-Sight MIMO for High Capacity Millimeter Wave Backhaul in FDD Systems

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Communications and Information Networks 2020年第2期5卷 177-193页

作者： Ye Xue Xuanyu Zheng Vincent K.N.lau Hong Kong University of Science and Tech.ology Hong Kong 999077China

Wireless backhaul is considered to be the key part of the future wireless network with dense small cell traffic and high capacity *** this paper,we focus on the design of a high spectral efficiency line-of-sight(LoS)multiple-input multiple-output(MIMO)system for millimeter wave backhaul using dual-polarized frequency division duplex(FDD).High spectral efficiency is very ch.llenging to ach.eve for the system due to various physical impairments such.as phase noise(PHN),timing offset(TO)as well as the poor condition number of the LoS *** this paper,we propose a holistic solution containing TO compensation,PHN estimation,precoder/decorrelator optimization of the LoS MIMO for wireless backhaul,and the interleaving of each.*** show that the proposed solution has a robust performance with end-to-end spectral efficiency of 60 bit/s/Hz for 88 MIMO.

关键词： line-of-sight MIMO wireless backhaul FDD timing synch.onization phase noise

Transmission of the Ych.omosome microdeletion to a baby boy conceived after in tracytoplasmic sperm injection

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

ch.nese Medical Journal 2001年第1期114卷 97-99页

作者： Tse JYM Yeung WSB lau EYL Ng EHY Tam PC So WWK Tang MHY lau ET Ho PC Department of Obstetrics and Gynecology The University of HongKongHongKongChina

Subfertility can be caused by acquired or genetic factors. Y ch.omosome microdeletion is one of the genetic factors associating with male infertility.1 Azoospermia factors (AZFa, AZFb and AZFc) have been mapped to different subregions in Yq11.2 So far, two gene families, RNA-binding motif (RBM) and deleted in azoospermia (DAZ) from interval 6, were proposed as candidate spermatogenesis genes for AZF.3,4 Recent studies demonstrated that microdeletions were detected at a frequency of 5% to 18% in the AZF region of oligospermic and azoospermic men.5-7 With the development of assisted reproductive tech.ologies, particularly intracytoplasmic sperm injection (ICSI), these men can now father a ch.ld and the genetic abnormalities in defective spermatozoa could be transmitted to future offspring. To examine the possible transmission of the Y-ch.omosome microdeletion to the offspring via ICSI treatment, we performed both cytogenetic and molecular analyses of the Y ch.omosome on both an infertile patient with Y ch.omosome microdeletion and his offspring.

关键词： sperm