Duplex micro RNAs assay based on target-triggered universal reporter hybridization

作     者：Yinan Wang Yue Sun Choiwan Lau Jianzhong Lu 

作者机构：School of Pharmacy Fudan University 

出 版 物：《Journal of Pharmaceutical Analysis》 (药物分析学报（英文版）)

年 卷 期：2018年第8卷第4期

页      面：265-270页

核心收录：

学科分类：0710[理学-生物学] 100208[医学-临床检验诊断学] 1007[医学-药学(可授医学、理学学位)] 1002[医学-临床医学] 0817[工学-化学工程与技术] 0703[理学-化学] 10[医学] 0702[理学-物理学] 

基　　金：financially supported by the National Science Foundation of China (Grant No. 21575029) 

主　　题：xMAP array Multiple microRNAs Target-triggered Universal reporter 

摘      要：In this paper, we designed and evaluated a duplex detection strategy for micro RNAs（mi RNAs） using universal probe-based target-triggered double hybridization and fluorescent microsphere-based assay system（x MAP array）. In the absence of target mi RNA, reporter DNA cannot hybridize stably with the immobilized capture DNA due to its low melting temperature. Only after adding target mi RNA, can reporter probe hybridize with capture probe to form a stable three-component complex. This targettriggered stable hybridization makes this method possible for highly selective and sensitive detection of multiple mi RNAs. We exemplified a quantitative detection of duplex mi RNAs with a limit of detection of40 p M. The x MAP array platform holds the potential of extending this approach to simultaneous detection of up to 100 mi RNA targets. Considering the simplicity, rapidity and multiplexing, this work promised a potential detection of multiple mi RNA biomarkers for early disease diagnosis and prognosis.