检索结果-南通市图书馆

Sterols possess immunomodulatory property:Activity guided isolation and in vitro screening of phytoconstituents of Pongamia glabra and Ficus glomerata

引用

Chinese Herbal Medicines 2020年第3期12卷 310-315页

作者： Sanjeev Heroor Arunkumar Beknal Nitin Mahurkar suresh hiremath Shivkumar Inamdar Department of Pharmacognosy HKES's MTR Institute of Pharmaceutical SciencesKalaburagi 585105India Deptartment of Pharmacology HKES's MTR Institute of Pharmaceutical SciencesKalaburagi 585105India

Objective:To isolate the phytoconstituents from the methanolic extracts of the stem bark of Pongamia glabra and Ficus glomerata,characterize spectroscopically and screen for in vitro immunomodulatory activity on human ***:A flavonoid(PGF)and an alkaloidal compound(PGA)from the extract of *** and a steroidal compound(FGS)and tannin fraction(FGT)from the extract of *** were isolated using column chromatography technique and were subjected for the spectroscopic(FT-IR,1HNMR and LC-MS)and TLC studies to identify the *** isolated compounds were screened for in vitro immunomodulatory activity on human neutrophils using nitroblue tetrazolium(NBT)dye test,phagocytosis of Candida albicans and neutrophil locomotion and chemotaxis assay at the concentration range of 100,50,25,12.50 and 5.00μg/***:From the spectroscopic and TLC studies data,the isolated compounds were identified as glabrin(PGA),karanjin(PGF),β-sitosterol(FGS),and tannin fraction(FGT).The isolated compounds PGA,PGF,FGS,and FGT exhibited significant(P<0.05)in vitro immunomodulatory activity in all the parameters ***:The steroidal compound,*** was found to be more immunopotent than all constituents alkaloid,flavonoid and ***,these constituents could be attributed to the immunomodulatory property of the plants.

关键词： activity guided isolation Ficus glomerate Roxb. immunomodulation Pongamia glabra Vent.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Decoding bovine coronavirus immune targets:an epitope informatics approach

引用

Animal Diseases 2024年第2期4卷 138-153页

作者： Swati Rani Mehnaj Khatoon Jagadish hiremath Kuralayanapalya Puttahonnappa suresh Jayashree Anandakumar Nagendra Nath Barman Sheethal Manjunath Yamini Sri S Sharanagouda S.Patil Spatial Epidemiology Lab ICAR-National Institute of Veterinary Epidemiology and Disease InformaticsYelahankaBengaluruKarnataka 560064India College of Veterinary Science Assam Agricultural UniversityGuwahatiAssam 785013India Yuvaraja’s College Mysuru University of MysuruMysuruKarnataka 570005India

Bovine coronavirus(BCoV)poses a significant threat to the global cattle industry,causing both respiratory and gastrointestinal infections in cattle *** necessitates the development of efficacious *** several inactivated and live BCoV vaccines exist,they are predominantly limited to *** immunization of adult cattle is imperative for BCoV infection control,as it curtails viral transmission to calves and ameliorates the impact of enteric and respiratory ailments across all age groups within the *** study presents an in silico methodology for devising a multiepitope vaccine targeting *** spike glycoprotein(S)and nucleocapsid(N)proteins,which are integral elements of the BCoV structure,play pivotal roles in the viral infection cycle and immune *** constructed a remarkably effective multiepitope vaccine candidate specifically designed to combat the BCoV *** immunoinformatics technology,B-cell and T-cell epitopes were predicted and linked together using linkers and adjuvants to efficiently trigger both cellular and humoral immune responses in *** in silico construct was characterized,and assessment of its physicochemical properties revealed the formation of a stable vaccine *** 3D modeling of the vaccine construct,molecular docking revealed a stable interaction with the bovine receptor ***,the viability of the vaccine’s high expression and simple purification was demonstrated by codon optimization and in silico cloning expression into the pET28a(+)*** applying immunoinformatics approaches,researchers aim to better understand the immune response to bovine coronavirus,discover potential targets for intervention,and facilitate the development of diagnostic tools and vaccines to mitigate the impact of this virus on cattle health and the livestock *** anticipate that the design will be useful as a preventive treatment for BCoV sickness in cattle,opening the door for furthe

关键词： Immunoinformatics Bovine coronavirus Multiepitope vaccine Molecular docking In silico cloning

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

一种抗偏头痛复方制剂体外溶出度的比较研究(英文)

引用

色谱 2010年第1期28卷 93-99页

作者： hiremath Vijay Basayya KALIAPERUMAL Krishna BHOJRAJ suresh NANJAN Mulla Joghee TIFAC CORE HD JSS College of PharmacyRocklands JSS University Banim antap

A novel in vitro dissolution profile was developed for formulated drug in combinational form containing naproxen sodium ( NAP) and sumatriptan succinate ( SUMA). This study was performed to understand the dissolution of the drug in the physiological temperature and pH. Dissolution testing was performed using USP 29 type II testing apparatus rotating at 50 r /min,in 900 mL deaerated buffer (pH 1. 2,4. 5 and 6. 8) which was maintained at ( 37 ± 0. 5) ℃. Quantification was performed using a developed and validated high performance liquid chromatographic (HPLC) method. Aceclofenac (ACE) was used as internal standard. SUMA,ACE and NAP w ere eluted at 4. 8,5. 7 and 7. 9 min,respectively. As expected for enteric coated immediate release (IR) tablets,the dissolution of NAP and SUMA was rapid and essentially complete within 2 h using phosphate buffer ( pH 6. 8). The comparison of the dissolution profiles was realized by model independent approach using a difference factor ( f1) ,similarity factor (f2) and dissolution efficiency ( DE). Statistical results show ed the pro-files were similar to the reference and the test products. Hence,this method demonstrated to be adequate for in vitro studies of NAP and SUMA in the combinational dosage form,since there is no official monograph,col-laborating to the official codes.

关键词：甲氧萘丙酸萘普生钠琥珀酸盐分解方法

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：