Sterols possess immunomodulatory property:Activity guided isolation and in vitro screening of phytoconstituents of Pongamia glabra and Ficus glomerata
Sterols possess immunomodulatory property: Activity guided isolation and in vitro screening of phytoconstituents of Pongamia glabra and Ficus glomerata作者机构：Department of PharmacognosyHKES's MTR Institute of Pharmaceutical SciencesKalaburagi 585105India Deptartment of PharmacologyHKES's MTR Institute of Pharmaceutical SciencesKalaburagi 585105India
出 版 物：《Chinese Herbal Medicines》 (中草药（英文版）)
年 卷 期：2020年第12卷第3期
页 面：310-315页
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
主 题：activity guided isolation Ficus glomerate Roxb. immunomodulation Pongamia glabra Vent.
摘 要：Objective:To isolate the phytoconstituents from the methanolic extracts of the stem bark of Pongamia glabra and Ficus glomerata,characterize spectroscopically and screen for in vitro immunomodulatory activity on human ***:A flavonoid(PGF)and an alkaloidal compound(PGA)from the extract of *** and a steroidal compound(FGS)and tannin fraction(FGT)from the extract of *** were isolated using column chromatography technique and were subjected for the spectroscopic(FT-IR,1HNMR and LC-MS)and TLC studies to identify the *** isolated compounds were screened for in vitro immunomodulatory activity on human neutrophils using nitroblue tetrazolium(NBT)dye test,phagocytosis of Candida albicans and neutrophil locomotion and chemotaxis assay at the concentration range of 100,50,25,12.50 and 5.00μg/***:From the spectroscopic and TLC studies data,the isolated compounds were identified as glabrin(PGA),karanjin(PGF),β-sitosterol(FGS),and tannin fraction(FGT).The isolated compounds PGA,PGF,FGS,and FGT exhibited significant(P0.05)in vitro immunomodulatory activity in all the parameters ***:The steroidal compound,*** was found to be more immunopotent than all constituents alkaloid,flavonoid and ***,these constituents could be attributed to the immunomodulatory property of the plants.
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