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A Novel Gene Delivery System Targeting Urokinase Receptor

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Acta Biochimica et Biophysica Sinica 2004年第7期36卷 485-491页

作者： Xing-Hui sun, li TAN, chun-Yang li, Chang TONG, Jin FAN, Ping li, and Yun-Song ZHU*Department of Molecular Genetics, Key Laboratory of Molecular Medicine of Ministry Education,Shanghai Medical College of Fudan University, Shanghai 200032, China Department of Molecular Genetics Key Laboratory of Molecular Medicine of Ministry Education Shanghai Medical College of Fudan UniversityShanghai 200032 China

Recombinant proteins that combine different functions required for cell targeting and intra- cellular delivery of DNA present an attractive approach for the development of nonviral gene delivery vectors. Here, we described a novel protein termed ATF-lys10 which facilitated cell-specific gene transfer via receptor-mediated endocytosis. ATF-lys10 was composed of the amino-terminal fragment of urokinase and ten lysines at the carboxyl terminus. Bacterially expressed ATF-lys10 protein existed in soluble form, and had antigenicity of human urokinase. Purified ATF-lys10 specifically bound to uPAR-expressing cells and formed protein-DNA complexes with plasmid pGL3-control. After neutralization of excess negative charge with poly-L-lysine, these complexes served as a specific gene delivery vector for uPAR-expressing cells. Lyso- somotropic compounds, such as chloroquine, drastically increased the ATF-lys10 mediated gene delivery efficiency. Our results suggest that the recombinant protein ATF-lys10 with the properties of DNA binding and tumor cell targeting represents a promising method for gene transfer and expression in tumor cells.

关键词： amino-terminal fragment urokinase gene transfer endocytosis urokinase receptor

Comparisons of multiple characteristics between young and old lung cancer patients

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Chinese Medical Journal 2012年第1期125卷 72-80页

作者： JIANG Wei KANG Yue SHI Guang-yue ZHANG Hai-yu CAI li sun Xiu-wei sun li-chun SUI Guang-jie Department of Medical Oncology the Third Affiliated Hospital of Harbin Medical University Harbin Heilongjiang 150081 China The Hospital of Harbin Engineering University Harbin Heilongjiang 150001 China Department of Surgery the Second Affiliated Hospital of Harbin Medical University Harbin Heilongjiang 150086 China Department of Biostatistics Harbin Medical University Harbin Heilongjiang 150086 China

Background Risk factors that contribute to younger patients with lung cancer are still relatively *** aim of this study was to compare the clinical characteristics,histological types,stages at diagnosis,treatment modalities and survival rates between young and old patients with lung *** The study was designed as a retrospective review of all lung cancer patients admitted to the Third Affiliated Hospital of Harbin Medical University from 1998 to *** analyses using univariate and multivariate approaches were performed to compare the survival rates between different age groups and to discover potential prognostic *** This research included 3320 patients with primary lung cancer,of whom 626 （18.8%） were 45 years old or younger at the time of *** percentage of smokers and the male to female ratios between the young and old patient groups were 51.27% vs.70.6% （P 〈0.001） and 1.99 vs.2.13 （P=0.4801）,*** young patient group had a higher incidence of adenocarcinoma and fewer *** 1-year,3-year and 5-year survival rates in the young patient group were generally lower than those of the old patient group,with significant differences （P=0.0232）.The clinical stage of the tumor was a prognostic factor for both non-small cell lung cancer patients （P 〈0.0001 ） and small cell lung cancer patients （P=0.0002）.Symptoms,diagnostic method,histology,smoking,treatment modality and body mass index were shown to have significant relationships with the survival of lung cancer patients （P 〈0.05）.Conclusions Patients with lung cancer who are younger than 45 years old might have a significantly poorer prognosis than that of older ***,diagnosis method,histology,smoking,treatment modality and body mass index can be independent prognostic factors for lung cancer.

关键词： lung neoplasm age factor epidemiology survival analysis prognosis

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《莽丛中》的女性主义解读

杭州师范大学学报（社会科学版） 2010年第3期32卷 85-88页

作者：孙立春杭州师范大学外国语学院浙江杭州310036

芥川小说《莽丛中》通过女主人公真砂的受辱事件,真实反映出女性在父权制社会的历史境遇。作为一名被侮辱和被戕害的女性,真砂既要遭受男权社会的生理和心理的双重压迫,还无法摆脱父权制话语的规范和制约。真砂实际上处于一种两难境地:... 详细信息

芥川小说《莽丛中》通过女主人公真砂的受辱事件,真实反映出女性在父权制社会的历史境遇。作为一名被侮辱和被戕害的女性,真砂既要遭受男权社会的生理和心理的双重压迫,还无法摆脱父权制话语的规范和制约。真砂实际上处于一种两难境地:如果认同父权制社会的角色定位,则势必会丧失自我的性别权利;如果违背父权制社会的伦理法权,又势必会沦为不贞或堕落的形象。真砂的哭诉反映了她反抗男性霸权的潜意识,预示着其对现实处境与性别意识的逐渐觉醒。

关键词：《莽丛中》性别话语女性主义

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左归丸治疗更年期综合征72例

上海中医药杂志 2001年第3期35卷 26-27页

作者：李莉孙竹春上海市杨浦区市东医院上海200090

为观察左归丸治疗更年期综合征的效果 ,对 72例患者采用左归丸治疗 ,2 0天为 1疗程 ,共治疗 3疗程。结果 :总有效率为 90 2 8% ;月经紊乱、汗出、潮热、情绪、睡眠等症状均有不同程度的改善。提示左归丸对更年期综合征有较好临床疗效。

关键词：更年期综合征左归丸辨证分型中医药治疗妇女

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山小橘化学成分研究

中药材 2016年第1期39卷 90-93页

作者：陈力许剑锋孙立春上海海洋大学食品学院上海201306

目的:研究山小橘的化学成分。方法:采用乙醇渗辘提取结合正反相色谱法进行分离纯化,根据波谱学方法,同时结合理化性质鉴定化合物的结构。结果:从山小橘中分离得到11个化合物,分别鉴定为:5,7,4'-三羟基-二氢黄酮醇(1)、香橙素(2)、反式-... 详细信息

目的:研究山小橘的化学成分。方法:采用乙醇渗辘提取结合正反相色谱法进行分离纯化,根据波谱学方法,同时结合理化性质鉴定化合物的结构。结果:从山小橘中分离得到11个化合物,分别鉴定为:5,7,4'-三羟基-二氢黄酮醇(1)、香橙素(2)、反式-二氢槲皮素(3)、顺式-二氢槲皮素(4)、山柰素(5)、槲皮素(6)、5,7,4'-三羟基-二氢黄酮醇-3-O-α-L-吡喃鼠李糖苷(7)、槲皮素-3-O-α-L-呋喃阿拉伯糖苷(8)、槲皮素-3-O-α-L-吡喃鼠李糖苷(9)、5,7,3',4'-四羟基黄酮-3-O-吡喃鼠李糖(1→6)吡喃葡萄糖苷(10)、5,7,3'-三羟基-4'-甲氧基黄酮-3-O-吡喃鼠李糖(1→6)吡喃葡萄糖苷(11)。结论:所有化合物均为首次从该植物中分离得到。

关键词：山小橘芸香科化学成分

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基于仿生设计的风力机塔架力学性能分析

热能动力工程 2018年第5期33卷 126-132页

作者：孙瑞李春陈文朴上海理工大学能源与动力工程学院

为提高塔架力学性能,提出一种仿生结构塔架并研究静态和动态结构特性。建立了1.5 MW风力机塔架有限元模型,通过仿棕榈植物的结构,将传统塔架模型设计成带类维管束腹板的锥筒塔架和带类维管束腹板多段式仿生塔架,并将计算结果与传统塔架... 详细信息

为提高塔架力学性能,提出一种仿生结构塔架并研究静态和动态结构特性。建立了1.5 MW风力机塔架有限元模型,通过仿棕榈植物的结构,将传统塔架模型设计成带类维管束腹板的锥筒塔架和带类维管束腹板多段式仿生塔架,并将计算结果与传统塔架进行了对比分析。结果表明：类维管束腹板使仿生塔架具有更强的抗弯性能,可有效降低塔架的塔尖位移,较之传统塔架减小了68%;传统塔架的应力集中在塔架根部,而仿生塔架能高效地分散载荷,最大应力下降19%,起到减载作用;仿生塔架的一阶固有频率较传统塔架下降0.246 Hz,能够避开塔架的共振频率范围。

关键词：风力机塔架仿生模态分析减载力学性能

Synthesis,separation and antifungal activity of diastereoisomers of 2,3,3a,4-tetra hydropyrido[b]thiopyran[4,3-c]pyrazole-2-carboxamide derivatives

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Synthesis,separation and antifungal activity of diastereoiso...

Boosting bidirectional sulfur conversion enabled by introducing boron-doped atoms and phosphorus vacancies in Ni2P for lithium-sulfur batteries

2011年全国药物化学学术会议——药物的源头创新

作者： XIE Suguang,LUO Wei,ZHANG Xin,sun li,GUO chun~* (School of Pharmaceutical Engineering,Shenyang Pharmaceutical University)

＜正＞The incidence of fungal infections has increased dramatically in recent *** triazoles antifungal agents which act by inhibiting cytochrome P450 now became the most rapidly expanding group of antifungal ***,their... 详细信息

关键词： tetrahydropyrido[b]thiopyran[4,3-c]pyrazole-2-carboxamide synthesis antifungal

来源： cnki会议评论

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Journal of Energy Chemistry 2025年第1期100卷 760-769页

作者： lin Peng Yu Bai Hang li Meixiu Qu Zhenhua Wang Kening sun Advanced Research Institute of Multidisciplinary Science Beijing Institute of Technology Beijing Key Laboratory of Chemical Power Source and Green Catalysis School of Chemistry and Chemical Engineering Beijing Institute of Technology

lithium-sulfur （li-S） batteries have gained great attention due to the high theoretical energy density and low cost,yet their further commercialization has been obstructed by the notorious shuttle effect and sluggish redox ***,we supply a strategy to optimize the electron structure of Ni2P by concurrently introducing B-doped atoms and P vacancies in Ni2P （Vp-B-Ni2P）,thereby enhancing the bidirectional sulfur *** study indicates that the simultaneous introduction of B-doped atoms and P vacancies in Ni2P causes the redistribution of electron around Ni atoms,bringing about the upward shift of d-band center of Ni atoms and effective d-p orbital hybridization between Ni atoms and sulfur species,thus strengthening the chemical anchoring for lithium polysulfides （liPSs） as well as expediting the bidirectional conversion kinetics of sulfur ***,theoretical calculations reveal that the incorporation of B-doped atoms and P vacancies in Ni2P selectively promotes li2S dissolution and nucleation ***,the li-S batteries with Vp-B-Ni2P-separators present outstanding rate ability of 777 m A h g-1at 5 C and high areal capacity of 8.03 mA h cm-2under E/S of 5μL mg-1and sulfur loading of 7.20 mg *** work elucidates that introducing heteroatom and vacancy in metal phosphide collaboratively regulates the electron structure to accelerate bidirectional sulfur conversion.

关键词： lithium sulfur batteries

Brain endothelial cyclic GMP-AMP synthase(cGAS)-stimulator of interferon genes(STING)signaling pathway in aging and neurodegeneration

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Neural Regeneration Research 2025年第7期20卷 2005-2007页

作者： Bryan sun Lulin li Jian Luo Palo Alto Veterans Institute for Research VA Palo Alto Health Care SystemPalo AltoCAUSA Polytrauma System of Care VA Palo Alto Health Care SystemPalo AltoCAUSA

The cyclic GMP-AMP synthase(cGAS)-stimulator of interferon genes(STING)signaling pathway has emerged as a key mediator of *** current studies primarily attribute its effects to neurons and glial cells,emerging research suggests that cGAS-STING signaling may play a critical role in cerebral vasculature,particularly in brain endothelial ***,studying the role 7of inflammation caused by the cGAS-STING pathway in brain endothelial cells could provide a more comprehensive understanding of neuroinflammatory disease and new avenues for therapeutic ***,we review the multifaceted role of global cGAS-STING signaling in various neurological and neuroinflammatory diseases and the potential contribution of cGAS-STING in brain endothelial cells.

关键词： stimulator interferon inflammation