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Occurrence of viable but non-culturable(VBNC)pathogenic bacteria in tap water of public places

Frontiers of Environmental Science & Engineering 2024年第3期18卷 115-129页

作者： Lizheng Guo Xinyan Xiao Kassim Chabi Yiting Zhang Jingjing Li su yao Xin Yu China National Research Institute of Food and Fermentation Industries Co. Ltd.China Center of Industrial Culture CollectionBeijing 100015China Key Laboratory of Urban Environment and Health Institute of Urban EnvironmentChinese Academy of SciencesXiamen 361021China College of Environment&Ecology Xiamen UniversityXiamen 361102China

Viable but non-culturable(VBNC)bacteria have been detected in source water and effluent of drinking water treatment processes,leading to significant underestimation of viable cell *** information exists on VBNC bacteria in tap water,particularly in public *** address this gap,a comprehensive nine-month study was conducted in a major city in south-eastern China,using culture-based and quantitative PCR with propidium monoazide(PMA)dye ***-five samples were collected from five representative public places(railway station,campus,hospital,shopping mall,and institution).The findings revealed that culturable bacteria represented only 0–17.51%of the viable 16S rRNA genes,suggesting that the majority of viable bacteria existed in an uncultured or VBNC ***,opportunistic pathogens such as Escherichia coli,Enterococcus faecalis,Pseudomonas aeruginosa,Salmonella sp.,and Shigella *** primarily detected as VBNC cells,with concentrations ranging from 1.03×10^(0)to 3.01×10^(3),1.20×10^(0)to 1.42×10^(2),1.32×10^(0)to 8.82×10^(0),1.00×10^(0)to 6.71×10^(1),and 2.07×10^(0)to 1.93×10^(2)cell equivalent/100 mL,*** *** was observed in tap water after prolonged stagnation,indicating potential risks associated with bacterial *** and temporal factors accounted for 17.1%and 26.0%,respectively,of the variation in tap water community structure during the sampling period,as revealed by 16S rRNA amplicon *** study provides quantitative insights into the occurrence of VBNC bacteria in tap water and highlights the need for more sensitive monitoring methods and microbial control techniques to enhance tap water safety in public locations.

关键词： VBNC Pathogenic bacteria PMA treatment Public tap water Community structure

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Evolution of nonmetallic inclusions in 80-t 9CrMoCoB large-scale ingots during electroslag remelting process

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International Journal of Minerals,Metallurgy and Materials 2024年第7期31卷 1525-1539页

作者： Shengchao Duan Min Joo Lee yao su Wangzhong Mu Dong Soo Kim Joo Hyun Park School of Mechanical and Materials Engineering North China University of TechnologyBeijing 100144China Department of Materials Science and Chemical Engineering Hanyang UniversityAnsan 15588Rep.of Korea Department of Materials Science and Engineering KTH Royal Institute of TechnologyBrinellvägen 23Stockholm 10044Sweden Department of Engineering Science and Mathematics Division of Materials ScienceLuleåUniversity of Technology97187 LuleåSweden Casting&Forging Business Unit Nuclear Business GroupDoosan EnerbilityChangwon 51711Rep.of Korea

In combination with theoretical calculations,experiments were conducted to investigate the evolution behavior of nonmetallic inclusions(NMIs)during the manufacture of large-scale heat-resistant steel ingots using 9CrMoCoB heat-resistant steel and CaF_(2)–CaO–Al_(2)O_(3)–SiO_(2)–B_(2)O_(3)electroslag remelting(ESR)-type slag in an 80-t industrial ESR *** main types of NMI in the consumable electrode comprised pure alumina,a multiphase oxide consisting of an Al_(2)O_(3)core and liquid CaO–Al_(2)O_(3)–SiO_(2)–MnO shell,and M_(23)C_(6)carbides with an MnS *** Al_(2)O_(3)and MnS inclusions had higher precipitation temperatures than the M_(23)C_(6)-type carbide under equilibrium and nonequilibrium solidification ***,inclusions can act as nucleation sites for carbide layer *** ESR process completely removed the liquid CaO–Al_(2)O_(3)–SiO_(2)–MnO oxide and MnS inclusion with a carbide shell,and only the Al_(2)O_(3)inclusions and Al_(2)O_(3)core with a carbide shell occupied the remelted *** M_(23)C_(6)-type carbides in steel were determined as Cr_(23)C_(6)based on the analysis of transmission electron microscopy *** substitution of Cr with W,Fe,or/and Mo in the Cr_(23)C_(6)lattice caused slight changes in the lattice parameter of the Cr_(23)C_(6)***,Cr_(21.34)Fe_(1.66)C_(6),(Cr_(19)W_(4)C_(6),Cr_(18.4)Mo_(4.6)C_(6),and Cr_(16)Fe_(5)Mo_(2)C_(6)can match the fraction pattern of Cr_(23)C_(6)*** Al_(2)O_(3)inclusions in the remelted ingot formed due to the reduction of CaO,SiO_(2),and MnO components in the liquid *** increased Al content in liquid steel or the higher supersaturation degree of Al_(2)O_(3)precipitation in the remelted ingot than that in the electrode can be attributed to the evaporation of CaF_(2)and the increase in CaO content in the ESR-type slag.

关键词： nonmetallic inclusion heat-resistant steel electroslag remelting M_(23)C_(6) carbide MnS inclusion supersaturation degree

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A facile strategy for large-scale production of 2D nanosheets exfoliated by three-roll milling

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Journal of Advanced Ceramics 2024年第1期13卷 11-18页

作者： Xin Wang Yang Xia Juntong Huang yao su Zhi Chen Linlin Chen Zhaohui Wu Zhijun Feng Huiyong Yang Xibao Li School of Materials Science and Engineering Nanchang Hangkong UniversityNanchang 330063China

Two-dimensional(2D)nanomaterials,such as graphene,MoS_(2),and MAX,have attracted increasing research attention in recent years due to their unique structural and performance ***,their complex production processes and equipment requirements are significant issues affecting their widespread ***,with an exfoliation strategy using three-roll milling,we present a simple,cost-effective,and extensible method to produce multilayer graphene,BN,MoS_(2),and Ti_(3)AlC_(2) *** roller and phenolic resin created three kinds of forces on the layered 2D materials,i.e.,shear forces,compressive forces,and adhesive forces,which exfoliated layered materials from their edges and surfaces into ***,the exfoliated materials were ultrasonically washed with alcohol,treated with ultrasonic vibration,and centrifuged to obtain 2D *** easy operation and high yield are attractive for research based on the construction of high-performance 2D nanosheet-based devices at low ***,the obtained multilayer graphene and MoS_(2) nanosheets were used as anode materials of sodium/potassium-ion batteries,respectively,to test their electrochemical *** performances are obtained compared with their primary bulk materials.

关键词： two-dimensional(2D)nanosheets exfoliation three-roll milling(TRM) sodium/potassium-ion batteries

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Artificial intelligence-based analysis of tumor-infiltrating lymphocyte spatial distribution for colorectal cancer prognosis

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Chinese Medical Journal 2024年第4期137卷 421-430页

作者： Ming Cai Ke Zhao Lin Wu Yanqi Huang Minning Zhao Qingru Hu Qicong Chen su yao Zhenhui Li Xinjuan Fan Zaiyi Liu Department of Radiology Guangdong Provincial People’s Hospital(Guangdong Academy of Medical Sciences)Southern Medical UniversityGuangzhouGuangdong 510080China Guangdong Provincial Key Laboratory of Artificial Intelligence in Medical Image Analysis and Application GuangzhouGuangdong 510080China Guangdong Cardiovascular Institute Guangdong Provincial People’s Hospital(Guangdong Academy of Medical Sciences)Southern Medical UniversityGuangzhouGuangdong 510080China Medical Research Institute Guangdong Provincial People’s Hospital(Guangdong Academy of Medical Sciences)Southern Medical UniversityGuangzhouGuangdong 510080China Department of Pathology The Third Affiliated Hospital of Kunming Medical UniversityYunnan Cancer HospitalYunnan Cancer CenterKunmingYunnan 650118China The Second School of Clinical Medicine Southern Medical UniversityGuangzhouGuangdong 510515China Institute of Computing Science and Technology Guangzhou UniversityGuangzhouGuangdong 510006China Department of Pathology Guangdong Provincial People’s Hospital(Guangdong Academy of Medical Sciences)Southern Medical UniversityGuangzhouGuangdong 510080China Department of Pathology The Sixth Affiliated Hospital of Sun Yat-sen UniversityGuangzhouGuangdong 510655China

Background:Artificial intelligence(AI)technology represented by deep learning has made remarkable achievements in digital pathology,enhancing the accuracy and reliability of diagnosis and prognosis *** spatial distribution of CD3^(+)and CD8^(+)T cells within the tumor microenvironment has been demonstrated to have a significant impact on the prognosis of colorectal cancer(CRC).This study aimed to investigate CD3_(CT)(CD3^(+)T cells density in the core of the tumor[CT])prognostic ability in patients with CRC by using AI ***:The study involved the enrollment of 492 patients from two distinct medical centers,with 358 patients assigned to the training cohort and an additional 134 patients allocated to the validation *** facilitate tissue segmentation and T-cells quantification in whole-slide images(WSIs),a fully automated workflow based on deep learning was *** the completion of tissue segmentation and subsequent cell segmentation,a comprehensive analysis was ***:The evaluation of various positive T cell densities revealed comparable discriminatory ability between CD3_(CT) and CD3-CD8(the combination of CD3^(+)and CD8^(+)T cells density within the CT and invasive margin)in predicting mortality(C-index in training cohort:0.65 vs.0.64;validation cohort:0.69 vs.0.69).The CD3_(CT) was confirmed as an independent prognostic factor,with high CD3_(CT) density associated with increased overall survival(OS)in the training cohort(hazard ratio[HR]=0.22,95%confidence interval[CI]:0.12–0.38,Psue regions in WSIs using AI *** CD3_(CT) confirmed as a stage-independent predictor for OS in CRC ***,CD3_(CT) shows promise in simplifying the CD3-CD8 system and facilitating its practical application in clinical settings.

关键词： Colorectal cancer Artificial intelligence Deep learning Digital pathology Prognosis Immune cells CD3 CD8 TME

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Photochromic radical states in 3D covalent organic frameworks with zyg topology for enhanced photocatalysis

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National Science Review 2024年第07期 177-188页

作者： Tian-Tian Ma Guo-Zhang Huang Xiao-Han Wang Yan Liang Run-Han Li Bin Wang su-Juan yao Jia-Peng Liao Shun-Li Li Yong Yan Ya-Qian Lan School of Chemistry South China Normal University Department of Chemistry Guangdong Provincial Key Laboratory of Catalytic Chemistry Southern University of Science and Technology

Covalent-organic frameworks（COFs） with photoinduced donor-acceptor（D-A） radical pairs show enhanced photocatalytic activity in principle. However, achieving long-lived charge separation in COFs proves challenging due to the rapid charge recombination. Here, we develop a novel strategy by combining[6 + 4] nodes to construct zyg-type 3D COFs, first reported in COF chemistry. This structure type exhibits a fused Olympic-rings-like shape, which provides a platform for stabilizing the photoinduced D-A radical pairs. The zyg-type COFs containing catalytically active moieties such as triphenylamine and phenothiazine（PTZ） show superior photocatalytic production rates of hydrogen peroxide（H2O2）. Significantly, the photochromic radical states of these COFs show up to 400% enhancement in photocatalytic activity compared to the parent states, achieving a remarkable H2O2synthesis rate of 3324 μmol g-1h-1, which makes the PTZ-COF one of the best crystalline porous photocatalysts in H2O2production. This work will shed light on the synthesis of efficient 3D COF photocatalysts built on topologies that can facilitate photogenerating D-A radical pairs for enhanced photocatalysis.

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多系统受累的间变性淋巴瘤激酶阳性组织细胞增生症1例

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中华病理学杂志 2024年第5期53卷 495-497页

作者：姚溯张芬陈玉刘艳辉南方医科大学附属广东省人民医院(广东省医学科学院)病理科广州510080

报道多系统受累的间变性淋巴瘤激酶(ALK)阳性组织细胞增生症1例。患者女,35岁。因发现左乳肿物10 d入院。镜下见乳腺组织内梭形细胞增生、交错排列,细胞核卵圆形,核分裂象罕见,胞质丰富、嗜碱或透亮,背景可见散在淋巴细胞浸润,及散在残... 详细信息

报道多系统受累的间变性淋巴瘤激酶(ALK)阳性组织细胞增生症1例。患者女,35岁。因发现左乳肿物10 d入院。镜下见乳腺组织内梭形细胞增生、交错排列,细胞核卵圆形,核分裂象罕见,胞质丰富、嗜碱或透亮,背景可见散在淋巴细胞浸润,及散在残存的脂肪细胞及乳腺导管。免疫组织化学染色显示异常增生的梭形细胞CD68、CD163、ALK阳性,广谱细胞角蛋白(CKpan)、p63、Langerin、S-100蛋白、CD34、β-catenin、结蛋白、Calponin、STAT6阴性。ALK荧光原位杂交(FISH)分离探针检测显示ALK基因有断裂。二代测序于DNA及RNA水平均检测到ALK::KIF5B基因融合。ALK阳性组织细胞增生症是新近收录于第5版WHO造血与淋巴组织肿瘤的罕见病例,是伴有ALK::KIF5B基因融合的独特亚型,常常伴有多系统受累,ALK抑制剂靶向治疗有效、但远期生存情况不明确。经验不足的医师容易因认识不足而误诊,因此需要充分认识其临床表现、病理特征与分子改变。

关键词： ALK 间变性淋巴瘤激酶组织细胞增生症

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探寻江户时代出版物背后的化妆文化

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文化创新比较研究 2024年第17期8卷 69-73页

作者：粟瑶浙江工商大学东方语言与哲学学院浙江杭州310018

日本平安时代,以贵族为中心的国风文化诞生,独特的化妆文化开始萌芽,日本贵族们奠定了白红黑传统化妆三原色。至平安时代后期,这种独特化妆手法在男性间开始流行,男子打扮成女性模样之风渐及公卿。同时,化妆作为一种身份地位的表现与权... 详细信息

日本平安时代,以贵族为中心的国风文化诞生,独特的化妆文化开始萌芽,日本贵族们奠定了白红黑传统化妆三原色。至平安时代后期,这种独特化妆手法在男性间开始流行,男子打扮成女性模样之风渐及公卿。同时,化妆作为一种身份地位的表现与权力的象征随着政权的更替,由公家向武家扩散,并且渗透到庶民阶层。至江户时代,化妆文化在武家礼法与身份制度的双重规制下,逐渐在女性中普及并成为她们有限度展现个性的方式,这也是江户时代女性化妆文化的独特魅力所在。在化妆文化的发展历程中,“往来物”、美容指南书和浮世绘等出版物扮演了重要角色。该文深入分析这些出版物的具体内涵,重点关注它们如何塑造和影响了化妆观念,从而揭示其背后所蕴含的丰富化妆文化。

关键词：江户时代化妆文化出版物女子用往来物化妆书浮世绘

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安徽庐江方言语气词研究

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巢湖学院学报 2024年第1期26卷 8-15,45页

作者：苏瑶郭辉安徽大学文学院安徽合肥230039 合肥师范附属小学第二小学安徽合肥230051 亳州学院中文与传媒系安徽亳州236800

研究根据语法意义的不同,将庐江方言语气词分为三类:事态语气词、情态语气词与疑问语气词。庐江方言语气词特色鲜明,有四大特点:数量总体多于普通话,十分丰富;用于陈述句的语气词少于普通话中用于陈述句的语气词,说明庐江人民不常用平... 详细信息

研究根据语法意义的不同,将庐江方言语气词分为三类:事态语气词、情态语气词与疑问语气词。庐江方言语气词特色鲜明,有四大特点:数量总体多于普通话,十分丰富;用于陈述句的语气词少于普通话中用于陈述句的语气词,说明庐江人民不常用平调表达感情,易采用有变化的升调或降调传递语气;语气词和语气之间并不是一一对应的,同一语气可以用不同语气词来表示,不同语气可以用同一语气词表示,具有很大的灵活性;语气词与其所在的江淮官话区保持较高的相似性,但是部分语气词较为独特,表现出相对的独立性。研究考察了部分庐江方言语气词来源,并指出了其语法化路径。

关键词：庐江方言句末语气词语用功能来源语法化

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不凝性气体对纳米通道内水分子流动传热影响的分子动力学模拟

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物理学报 2024年第9期73卷 168-178页

作者：邢赫威陈占秀杨历苏瑶李源华呼和仓河北工业大学能源与环境工程学院天津300401

随着电子元件高性能化和小型化的发展,纳米通道内工质的流动传热问题受到了更多的关注.本文采用分子动力学模拟方法,模拟了300,325,350 K的纳米通道中流体的流动传热情况,工质为水,水中不凝性气体用氩气代替.结果表明:流动过程中,氩原... 详细信息

随着电子元件高性能化和小型化的发展,纳米通道内工质的流动传热问题受到了更多的关注.本文采用分子动力学模拟方法,模拟了300,325,350 K的纳米通道中流体的流动传热情况,工质为水,水中不凝性气体用氩气代替.结果表明:流动过程中,氩原子形成高势能团簇,随着温度升高,流体势能上升,团簇逐渐减小或消失;少量气体原子能够促进流动,而较多氩气会导致通道中心区域形成较大气体团簇而阻碍流动,同时,被加热的工质能显著减小流动阻力系数;近壁面区域流体温度高于中心区域,团簇内部原子活动更加剧烈,平均分子动能更大,温度更高;水的氢键结构可以促进纳米通道内的传热,氩原子会影响氢键数量,高温会破坏水分子形成的氢键网络,使努塞尔数下降.本研究分析了不凝性气体影响下微通道内水分子流动传热的机理,为电子设备的强化传热提供了理论指导.

关键词：分子动力学流动传热团簇纳米通道

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童年创伤与神经质之间的关系:基于性别的视角

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中国临床心理学杂志 2024年第2期32卷 387-393,399页

作者：苗正淼陈奇虹刘苏瑶顾晓塽唐美倩蚁金瑶中南大学湘雅二医院医学心理中心长沙410011 中南大学医学心理研究所长沙410011 国家精神心理疾病临床医学研究中心长沙410011

目的:探究童年创伤与神经质之间的关系,以及性别因素对两者关系的调节作用。方法:1285名临床心理科门诊患者完成了儿童期创伤问卷简表和大五人格量表修订版神经质分量表。将童年创伤不同类型和神经质不同特质作为节点纳入,使用R软件进... 详细信息

目的:探究童年创伤与神经质之间的关系,以及性别因素对两者关系的调节作用。方法:1285名临床心理科门诊患者完成了儿童期创伤问卷简表和大五人格量表修订版神经质分量表。将童年创伤不同类型和神经质不同特质作为节点纳入,使用R软件进行网络分析。结果:总样本中,情感虐待是强度和桥强度最高的创伤类型,抑郁是强度最高的神经质特质。男性样本中,冲动性与愤怒性敌意特质是重要桥接节点;而女性样本中,自我意识与抑郁特质是重要桥接节点。结论:尽早识别和干预情感虐待并重点关注儿童期受虐者的抑郁特质具有重要意义。此外,应重点关注男性受虐者与外化情绪相关的特质和女性受虐者与内化情绪相关的特质,进行差异化干预。

关键词：童年创伤儿童期虐待神经质性别差异网络分析

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