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American Journal of Analytical chemistry 2012年第1期3卷 67-75页

作者： christophe Y.Fischer Pascal Laurent stephanie Heuskin Delphine Durieux Eric Haubruge Francois J.Verheggen Georges c.Lognay Department of Analytical chemistry Gembloux Agro-Bio TechLiege UniversityLiegeBelgium Department of General and Organic chemistry Gembloux Agro-Bio TechLiege UniversityLiegeBelgium Department of Biological and Industrial chemistry Gembloux Agro-Bio TechLiege UniversityLiegeBelgium Department of Functional and Evolutionary Entomology Gembloux Agro-Bio TechLiege UniversityLiegeBelgium

These last few years the multicolored Asian ladybird, Harmonia axyridis (Pallas), previously introduced in Europe and North America as a biological control agent, has swiftly spread out on those territories and turns out to be a pest in several ways. In order to improve understanding of several key points of the biology of this ladybird, a novel fast-Gc method of quantification of harmonine, the main defense alkaloid of H. axyridis, has been designed and validated using the accuracy profile concept for concentrations ranging from 13 to 264 μg/individual. This method allows quantifying harmonine in a single insect with a maximal error risk of 20%. For ladybirds collected in spring, mean harmonine concentration observed was 106.6 μg/individual with a very high standard deviation of 80.2 μg/individual, which can easily be explained by the strong asymmetry of the results distribution. Males and females seem equally chemically protected as the harmonine concentration did not significantly differ between sexes. This method is currently used to improve our comprehension of several key points of H. axyridis biology including aggregation behavior and aposematism.

关键词： Alkaloid Harmonine Harmonia axyridis Fast-Gc Validation

脑组织中高浓度的HIV-1DNA水平将HAART后AIDS痴呆综合征或心血管病的AIDS人与普通AIDS人区别开

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中国科学（c辑） 2009年第7期39卷 662-668页

作者：赵丽 Derek c.GALLIGAN Susanna L.LAMERS YU stephanie Lamia SHAGRUN Marco SALEMI Michael S.McGRATH 山东大学公共卫生学院病毒学检验室 Department of Laboratory Medicine Positive Health ProgramUniversity of CaliforniaSan FranciscoCalifornia94110USA Gene Johnson Inc. St.AugustineFlorida32080USA Pathologica Inc. BurlingameCalifornia94010USA Department of Pathology Immunology and Laboratory MedicineUniversity of FloridaGainesvilleFlorida32610USA

高效抗逆转录病毒治疗(highly active antiretroviral treatment,HAART)在延长获得性免疫缺陷综合征(acquired immunodeficiency syndrome,AIDS)患者的生存方面具有重要作用,然而随着HIV感染者寿命的延长,AIDS痴呆综合征(AIDS dementiacomplex,ADc),其他非AIDS相关疾病,心血管病(cardiovascular disease,cVD)也比较常见.这些各种疾病对脑组织中HIV-1DNA的影响在HAART后还未完全搞清楚.本文的研究目的是澄清HAART后ADc以及其他HIV感染的并发症对AIDS病人脑组织中病毒载量的影响.本实验用定量PcR研究了13例AIDS病人脑组织尸检标本,这些病人死于AIDS并发症,除1例病人外,其他12例病人均从1995年开始接受HAART治疗,直到死亡.2例死于严重的cVD,脑组织中广泛的动脉粥样硬化(cerebrovascular atherosclerosis,cVA).5例AIDS病人死于ADc,6例AIDS人既无ADc,也无cVA.用定量PcR检测了病人脑组织6个部位(脑膜、前叶灰质、前叶白质、颞叶皮层下部、小脑和基底节)HIV-1DNA的含量并对其进行了比较.HAART后,与非ADc/cVA的AIDS病人相比,ADc的AIDS病人脑组织中HIV-1DNA含量明显升高.一个新的发现是,2例患有cVD,特别是cVA的病人,脑组织中,也同样检出高浓度的HIV-1DNA,这些病人并没有ADc的症状,这是首次报道cVA与脑组织中HIV-1病毒载量有关.研究表明,HAATR抗性的HIV病毒储存库可能存在于AcD患者脑组织的损伤部位及富含巨噬细胞的动脉粥样硬化斑块中,仍需要大量的cVA病例研究来证实后者.

关键词：人类免疫缺陷病毒-1型 AIDS痴呆综合征定量PcR DNA 心血管病脑血管动脉粥样硬化

Performance Analysis of Robotic Arm Manipulators control System under Multitasking Environment

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Journal of Mechanics Engineering and Automation 2012年第5期2卷 327-331页

作者： Adnan Al Moshi Salwa Salam cynthia Eftakhairul Islam Rumana Rahman Akm Abdul malek Azad Department of Electrical and Electronic Engineering BRA C University Dhaka 1212 Bangladesh

This work is to observe the performance of Pc based robot manipulator under general purpose （Windows）, Soft （Linux） and Hard （RT Linux） Real Time Operating Systems （OS）. The same open loop control system is observed in different operating systems with and without multitasking environment. The Data Acquisition （DAQ, PLc-812PG） card is used as a hardware interface. From the experiment, it could be seen that in the non real time operating system （Windows）, the delay of the control system is larger than the Soft Real Time OS （Linux）. Further, the authors observed the same control system under Hard Real Time OS （RT-Linux）. At this point, the experiment showed that the real time error （jitter） is minimum in RT-Linux OS than the both of the previous OS. It is because the RT-Linux OS kernel can set the priority level and the control system was given the highest priority. The same experiment was observed under multitasking environment and the comparison of delay was similar to the preceding evaluation.

关键词： control system DAQ (data acquisition) card jitter multitasking RT-Linux.

High-rate intercity quantum key distribution with a semiconductor single-photon source

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Light(Science & Applications) 2024年第10期13卷 2114-2123页

作者： Jingzhong Yang Zenghui Jiang Frederik Benthin Joscha Hanel Tom Fandrich Raphael Joos stephanie Bauer Sascha Kolatschek Ali Hreibie Eddy Patrick Rugeramigabo Michael Jetter Simone Luca Portalupi Michael Zopf Peter Michler tefan Kuck Fei Ding institut fur Festkorperphysik Leibniz Universitat HannoverAppelstraBe 230167 HannoverGermany institut fur Halbleiteroptik und Funktionelle Grenzflachen Center for Integrated Quantum Science and Technology(IQST)and SCoPEUniversity of StuttgartStuttgartGermany Physikalisch-Technische Bundesanstalt BraunschweigGermany Laboratorium fur Nanound Quantenengineering Leibniz Universitat HannoverSchneiderberg 3930167HannoverGermany

Quantum key distribution(QKD)enables the transmission of information that is secure against general attacks by *** use of on-demand quantum light sources in QKD protocols is expected to help improve security and maximum tolerable *** quantum dots(QDs)are a promising building block for quantum communication applications because of the deterministic emission of single photons with high brightness and low multiphoton *** we report on the first intercity QKD experiment using a bright deterministic single photon source.A BB84 protocol based on polarisation encoding is realised using the high-rate single photons in the telecommunication c-band emitted from a semiconductor QD embedded in a circular Bragg grating *** the 79 km long link with 25.49 dB loss(equivalent to 130 km for the direct-connected optical fibre)between the German cities of Hannover and Braunschweig,a record-high secret key bits per pulse of 4.8 x io-5 with an average quantum bit error ratio of~0.65%are *** asymptotic maximum tolerable loss of 28.11 dB is found,corresponding to a length of 144 km of standard telecommunication *** semiconductor sources therefore challenge state-of-the-art QKD protocols and have the potential to excel in measurement device independent protocols and quantum repeater applications.

关键词： structure. quantum distribution

Single Nucleotide Polymorphism Identification in Polyploids： A Review, Example, and Recommendations

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Molecular Plant 2015年第6期8卷 831-846页

作者： Josh clevenger carolina chavarro stephanie A. Pearl Peggy Ozias-Akins Scott A. Jackson center for AppLied Genetic Technologies University of Georgia Athens GA 30602 USA Institute of Plant Breeding Genetics & Genomics University of Georgia Tifton GA 31793 USA These authors contributed equally to this article.

Understanding the relationship between genotype and phenotype is a major biological question and being able to predict phenotypes based on molecular genotypes is integral to molecular breeding. Whole- genome duplications have shaped the history of all flowering plants and present challenges to elucidating the relationship between genotype and phenotype, especially in neopolyploid species. Although single nucleotide polymorphisms （SNPs） have become popular tools for genetic mapping, discovery and appli- cation of SNPs in polyploids has been difficult. Here, we summarize common experimental approaches to SNP calling, highlighting recent polyploid successes. To examine the impact of software choice on these analyses, we called SNPs among five peanut genotypes using different alignment programs （BWA-mem and Bowtie 2） and variant callers （SAMtools, GATK, and Freebayes）. Alignments produced by Bowtie 2 and BWA-mem and analyzed in SAMtools shared 24.5% concordant SNPs, and SAMtools, GATK, and Freebayes shared 1.4% concordant SNPs. A subsequent analysis of simulated Brassica napus chromosome 1A and 1c genotypes demonstrated that, of the three software programs, SAMtools performed with the highest sensitivity and specificity on Bowtie 2 alignments. These results, however, are likely to vary among species, and we therefore propose a series of best practices for SNP calling in polyploids.

关键词： genomics homeolog next-generation sequencing (NGS) peanut polyptoid single nucleotide poly-morphism (SNP)

DNA metabarcoding reveals seasonal changes in diet composition across four arthropod-eating lizard species(Phrynosomatidae:Sceloporus)

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Integrative Zoology 2024年第3期19卷 480-495页

作者： Mauricio HERNÁNDEZ stephanie HEREIRA-PAcHEcO Antton ALBERDI Aníbal H.DÍAZ DE LA VEGA-PÉREZ Arturo ESTRADA-TORRES Sergio ANcONA Yendi E.NAVARRO-NOYA Doctorado en ciencias Biológicas Centro Tlaxcala de Biología de la ConductaUniversidad Autónoma de TlaxcalaTlaxcalaMexico Estación científica La Malinche Centro Tlaxcala de Biología de la ConductaUniversidad Autónoma de TlaxcalaTlaxcalaMexico center for Evolutionary Hologenomics The Globe InstituteUniversity of CopenhagenCopenhagenDenmark consejo Nacional de ciencia y Tecnología-centro Tlaxcala de Biología de la conducta Universidad Autónoma de TlaxcalaTlaxcalaMexico Departamento de Ecología Evolutiva Instituto de EcologíaUniversidad Nacional Autónoma de MéxicoMexico CityMexico Laboratorio de Interacciones Bióticas Centro de Investigación en Ciencias BiológicasUniversidad Autónoma de TlaxcalaSan Felipe IxtacuixtlaTlaxcalaMexico

Diet composition and its ecological drivers are rarely investigated in coexisting closely related *** used a molecular approach to characterize the seasonal variation in diet composition in four spiny lizard species inhabiting a mountainous *** metabarcoding revealed that the lizards Sceloporus aeneus,***,***,and *** mostly consumed arthropods of the orders Hemiptera,Araneae,Hymenoptera,and *** terrestrial lizards *** and *** mostly predated ants and spiders,whereas the arboreal–saxicolous *** and saxicolous *** largely consumed grasshoppers and *** taxonomic and phylogenetic diversity of the prey was higher during the dry season than the rainy season,likely because reduced prey availability in the dry season forced lizards to diversify their diets to meet their nutritional *** and phylogenetic composition varied seasonally depending on the species,but only dietary composition varied with *** dietary turnover was greater in *** than in ***,suggesting site-specific seasonal variability in prey availability;no other differences among species were ***,which lives at the highest altitude in our study site,displayed interseasonal variation in diet *** differences were correlated with the species’feeding strategies and elevational distribution,which likely contributed to the coexistence of these lizard species in the studied geographic area and beyond.

关键词： arthropod-eating lizards diet breadth environmental barcoding mountainous ecosystem seasonal dietary shifts

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在香港看到的“耀中幼教教学法”

早期教育（幼教·教育教学） 2020年第6期 17-19页

作者： stephanie c.Sanders-Smith 刘萌然美国伊利诺伊大学香槟分校新西兰奥克兰大学

香港“耀中幼教教学法”,是由陈保琼博士自20世纪70年代从美国学成归国,构建“游戏中学习”的早期教育课程开始,经过50年的实践,逐渐形成的系统的教学法。在香港耀中幼儿园,我们看到了耀中幼教教学法的实践情形。每天入学、放学时,你会... 详细信息

香港“耀中幼教教学法”,是由陈保琼博士自20世纪70年代从美国学成归国,构建“游戏中学习”的早期教育课程开始,经过50年的实践,逐渐形成的系统的教学法。在香港耀中幼儿园,我们看到了耀中幼教教学法的实践情形。每天入学、放学时,你会看到穿着耀中园服进进出出的幼儿园孩子,色彩和谐、式样俊美的园服,让孩子们看上去活力四射。但更让我们好奇的不是这些,而是在耀中幼儿园,孩子们究竟是怎样度过一天又一天的。

关键词：色彩和谐幼教耀中活力四射教学法幼儿园早期教育课程游戏中学习

Methylenetetrahydrofolate reductase c677T genotype affects promoter methylation of tumor-specific genes in sporadic colorectal cancer through an interaction with folate/vitamin B_(12) status

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World Journal of Gastroenterology 2008年第23期14卷 3662-3671页

作者： Pooneh Mokarram Fakhraddin Naghibalhossaini Mehdi Saberi Firoozi Seyed Vahid Hosseini Ahmad Izadpanah Heshmetalah Salahi Seyed Ali malek-Hosseini Abdoulrasool Talei Mehra Mojallal Department of Biochemistry Shiraz University of Medical SciencesSchool of MedicineShiraz 71345Iran Department of Internal Medicine and Gastroenterohepatology Research centre Shiraz University of Medical SciencesShiraz 71345Iran Department of Surgery(colorectal ward) and Gastroenterohepatology Research centre Shiraz University of Medical SciencesShiraz 71345Iran Department of Surgery and Organ Transplantation Research centre Namazee HospitalShiraz University of Medical SciencesShiraz 71345Iran Department of Surgery and Institute of cancer Research Shiraz University of Medical SciencesShiraz 71345Iran Pathology Laboratory Dena HospitalShiraz 71345Iran

AIM:To evaluate joint effects of Methylentetra-hydrofolate reductase(MTHFR) c677T genotypes,and serum folate/vitamin B12 concentrations on promoter methylation of tumor-associated genes among Iranian colorectal cancer patients. METHODS:We examined the associations between MTHFR c677T genotype,and promoter methylation of P16,hMLH1,and hMSH2 tumor-related genes among151 sporadic colorectal cancer patients. The promoter methylation of tumor-related genes was determined by methylation-specific PcR. Eighty six patients from whom fresh tumor samples were obtained and 81 controls were also examined for serum folate and vitamin B12 concentrations by a commercial radioimmunoassay kit. RESULTS:We found 29.1% of cases had tumors with at least one methylated gene promoter. In case-case comparison,we did not find a significant association between methylation in tumors and any single genotype. However,in comparison to controls with the cc genotype,an increased risk of tumor methylation was associated with the cT genotype(OR = 2.5;95% cI,1.1-5.6) . In case-case comparisons,folate/vitamin B12 levels were positively associated with tumor methylation. Adjusted odds ratios for tumor methylation in cases with high(above median) versus low(below median) serum folate/vitamin B12 levels were 4.9(95% cI,1.4-17.7) ,and 3.9(95% cI,1.1-13.9) ,respectively. The frequency of methylated tumors was significantly higher in high methyl donor than low methyl donor group,especially in those with MTHFR cT(P = 0.01) ,and cT/TT(P = 0.002) genotypes,but not in those with the cc genotype(P = 1.0) . cONcLUSION:We conclude that high concentrations of serum folate/vitamin B12 levels are associated with the risk of promoter methylation in tumor-specific genes,and this relationship is modified by MTHFR c677T genotypes.

关键词： Metthylentetrahydrofolate reductase Folate Vitamin 1322 Methylation colorectal cancer

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大肿块ⅠB、ⅡA期及局部晚期宫颈癌的治疗

中国癌症杂志 2006年第11期16卷 890-895页

作者： stephanie c.Han 章青陆嘉德美国纽约Maimonides医院肿瘤治疗中心妇科上海交通大学附属第六人民医院放疗科上海200233 The cancer Institute National Healthcare GroupSingapore

宫颈癌是全球范围内严重威胁人类身体健康的疾病,也是中国最常见的妇科恶性肿瘤之一。在尚未开展宫颈涂片普查的国家和地区,患者就诊时肿瘤通常已发展至大肿块ⅠB/Ⅱ期或局部晚期宫颈癌。宫颈癌最常应用的分期系统是FIGO分期系统。尽管... 详细信息

宫颈癌是全球范围内严重威胁人类身体健康的疾病,也是中国最常见的妇科恶性肿瘤之一。在尚未开展宫颈涂片普查的国家和地区,患者就诊时肿瘤通常已发展至大肿块ⅠB/Ⅱ期或局部晚期宫颈癌。宫颈癌最常应用的分期系统是FIGO分期系统。尽管分期为早期,但大肿块ⅠB/Ⅱ期宫颈癌的预后与局部晚期患者类似,且单纯子宫切除术无法治愈这类患者。外放射后继以腔内近距离放射联合同期DDP为主的化疗可有效提高局部晚期宫颈癌的总生存率。本文将就目前支持联合治疗大肿块ⅠB/Ⅱ期或局部晚期宫颈癌的临床试验结果进行全面评估。

关键词：宫颈肿瘤局部晚期大肿块综合治疗放疗

The Fate of Aflatoxin in corn Fermentation

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Natural Resources 2012年第3期3卷 126-136页

作者： c. Ian Johnston Rebecca Singleterry cedric Reid Darrell Sparks Ashli Brown Brian Baldwin stephanie Hill Ward W. Paul Williams corn Host Plant Resistance Research Unit United States Department of Agriculture Agricultural Research Service Washington DC USA Department of Animal and Dairy Science Mississippi State University Oktibbeha County USA Department of Biochemistry Molecular Biology Entomology and Plant Pathology Mississippi State University Oktibbeha County USA Department of Plant and Soil Sciences Mississippi State University Oktibbeha County USA

A lab-scale ethanol fermentation was investigated to determine where aflatoxin concentrated during each phase of production. Four corn samples with high levels of aflatoxin (ranging from 7750 – 17,208 parts per billion) and their replicates were compared with a replicated negative control. Fractions were taken from the fermented mash, distilled ethanol, stillage, and dried corn solids (DcS). These fractions were analyzed using two different immunoassay methods and liquid chromatography tandem mass spectrometry (Lc-MS/MS). Results indicated no aflatoxin was found in the distilled ethanol. Some aflatoxin (13%) was detected in the stillage, but most of the toxin was recovered in the DcSs ranging from 31% to 58%. A second series of experiments were conducted to investigate the effect of binders on dried distillers grains (DDGs). A brewers dried yeast anti-caking binder that contains glucomannon (MTB-100?), was mixed with contaminated DDGs. Addition of the binder showed a significant reduction in aflatoxin levels in comparison to a positive control. Aflatoxin binding at 2% binder w/w reached 72.5% and showed a minimal binding percentage increase of 80% at 6% binder w/w. Testing was also conducted to determine if environmental variables such as pH and temperature had any effect on the binding capabilities. Temperature near 0?c resulted in binding at 19.7% at a pH range of 6 to 8. Additionally, at a temperature of 40?c resulted in binding of 36%, 47%, and 45% at pHs 6, 7, and 8, respectively. These findings suggest that the addition of sorbents may be an effective way of salvaging contaminated DDGs.

关键词： Lc-MS/MS Ethanol Immunoassay Distillers Grains Binders