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Decellularized extracellular matrix as scaffold for cancer organoid cultures of colorectal peritoneal metastases

Journal of Molecular Cell Biology 2022年第11期14卷 36-49页

作者： Luca Varinelli Marcello Guaglio Silvia Brich Susanna Zanutto Antonino Belfiore Federica Zanardi Fabio Iannelli Amanda Oldani Elisa Costa Matteo Chighizola Ewelina Lorenc Simone P.Minardi stefano Fortuzzi Martina Filugelli Giovanna Garzone Federica Pisati Manuela Vecchi Giancarlo Pruneri Shigeki Kusamura Dario Baratti Laura Cattaneo Dario Parazzoli Alessandro Podestà Massimo Milione Marcello Deraco Marco A.Pierotti Manuela Gariboldi Department of Research Fondazione IRCCS Istituto Nazionale dei Tumorivia G.Venezian 120133 MilanItaly Peritoneal Surface Malignancies Unit Colon and Rectal SurgeryFondazione IRCCS Istituto Nazionale dei Tumorivia G.Venezian 120133 MilanItaly Department of Pathology and Laboratory Medicine Clinical Research lABoratory(CRAB)Fondazione IRCCS Istituto Nazionale dei Tumorivia G.Venezian 120133 MilanItaly Bioinformatics Core Unit IFOM ETSThe AIRC Institute of Molecular Oncologyvia Adamello 1620139 MilanItaly Imaging Technological Development Unit IFOM ETSThe AIRC Institute of Molecular Oncologyvia Adamello 1620139 MilanItaly C.I.Ma.I.Na.and Dipartimento di Fisica‘Aldo Pontremoli’ Universitàdegli Studi di Milanovia G.Celoria 1620133 MilanItaly Cogentech Ltd Benefit Corporation with a Sole Shareholder via Adamello 1620139 MilanItaly Pathology and Laboratory Medicine Department Fondazione IRCCS Istituto Nazionale dei Tumorivia G.Venezian 120133 MilanItaly

Peritoneal metastases (PM) from colorectal cancer (CRC) are associated with poor survival. The extracellular matrix (ECM) plays a fundamental role in modulating the homing of CRC metastases to the peritoneum. The mechanisms underlying the interactions between metastatic cells and the ECM, however, remain poorly understood, and the number of in vitro models available for the study of the peritoneal metastatic process is limited. Here, we show that decellularized ECM of the peritoneal cavity allows the growth of organoids obtained from PM, favoring the development of three-dimensional (3D) nodules that maintain the characteristics of in vivo PM. Organoids preferentially grow on scaffolds obtained from neoplastic peritoneum, which are characterized by greater stiffness than normal scaffolds. A gene expression analysis of organoids grown on different substrates reflected faithfully the clinical and biological characteristics of the organoids. An impact of the ECM on the response to standard chemotherapy treatment for PM was also observed. The ex vivo 3D model, obtained by combining patient-derived decellularized ECM with organoids to mimic the metastatic niche, could be an innovative tool to develop new therapeutic strategies in a biologically relevant context to personalize treatments.

关键词： colorectal cancer peritoneal metastasis organoids extracellular matrix(ECM) decellularized extracellular matrix engineered disease model ECM stiffness

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三种全肠道注洗法准备肠道的比较

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国际护理学杂志 1982年第1期03卷 26-26页

作者： stefano Minervinl ,魏建东

利用传统的肠道准备法常需时2～3天并限制饮食,可能造成脱水及体质消耗。作者改用全肠道注洗法。1978～1979年对做大肠择期手术和结肠镜检的62名病人分三组进行了研究。A组经鼻胃管单纯灌注盐水;B组口服甘露醇+经鼻胃管灌注盐水;C组单... 详细信息

利用传统的肠道准备法常需时2～3天并限制饮食,可能造成脱水及体质消耗。作者改用全肠道注洗法。1978～1979年对做大肠择期手术和结肠镜检的62名病人分三组进行了研究。A组经鼻胃管单纯灌注盐水;B组口服甘露醇+经鼻胃管灌注盐水;C组单纯口服甘露醇。准备日以前不需禁食。当日所有病人均插尿管以定时验尿。临注洗前给灭吐灵10mg(metoclopramide),开始注洗后2小时再用一次。A、B两组通过鼻胃管(B组在盐水注洗前先口服10%甘露醇500ml),往入37℃的155mmol/升浓度的温盐水。开始速率3升/小、时,注洗时病人坐于便桶上,直至排泄物中无粪便后

关键词：注洗盐水甘露醇病人多元醇内窥镜检结肠镜检肠道准备

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尿中核苷排放模式测定的两种方法─-HPLC和CE法(英文)

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色谱 1999年第2期17卷 97-101页

作者：许国旺路鑫张玉奎卢佩章中国科学院大连化学物理研究所色谱开放实验室

癌症病人在尿中排放比常人高得多的叶啉和嘧啶，这些改性核耷是RNA的主要组成。由于其增加的排放与癌变的RNA周转有关，已被建议和研究作肿瘤标记物。文章给出测定尿中核苷的反相高效液相色谱法和毛细管电泳方法。两种方法所得数据一致... 详细信息

癌症病人在尿中排放比常人高得多的叶啉和嘧啶，这些改性核耷是RNA的主要组成。由于其增加的排放与癌变的RNA周转有关，已被建议和研究作肿瘤标记物。文章给出测定尿中核苷的反相高效液相色谱法和毛细管电泳方法。两种方法所得数据一致。用此方法建立了正常人尿中核苷的排放水平，并测定了34个癌症病人尿中核苷浓度，观察到改性核着浓度明显的增高现象。结果袭明，两个方法均适于大量尿样的核苷与癌症关系的临床研究。

关键词： HPLC CE 核苷肿瘤标记物癌症尿样测定

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Contracaecum ogmorhini DNA多态性的研究

Contracaecum ogmorhini DNA多态性的研究

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中国动物学会全国第九次寄生虫学学术讨论会

作者：陈红玲 stefano D’Amelio 林瑞庆 Lia Paggi 宋慧群李国清 Robin B.Gasser 朱兴全华南农业大学兽医学院 Istituto di Parassitologia Universita di Roma "La Sapienza" Rome Italy 华南农业大学兽医学院 Istituto di Parassitologia Universita di Roma "La Sapienza" Rome Italy 华南农业大学兽医学院华南农业大学兽医学院 Department of Veterinary Science The University of Melboume Victoria Australia 华南农业大学兽医学院

＜正＞欧氏对盲囊线虫（Contracaecum ogmorhini）是水生动物的一种常见蛔虫科线虫。在分类学上,它属于线虫纲、蛔虫总科、异尖线虫科、对盲囊线虫属。本项研究以***作为研究对象,旨在研究来自南、北半球的***三个不同地理株在线粒体...

＜正＞欧氏对盲囊线虫（Contracaecum ogmorhini）是水生动物的一种常见蛔虫科线虫。在分类学上,它属于线虫纲、蛔虫总科、异尖线虫科、对盲囊线虫属。本项研究以***作为研究对象,旨在研究来自南、北半球的***三个不同地理株在线粒体基因序列是否存在差异,以及差异程度的大小,并对其种群间的遗传变异和序列同源性做进一步的分析和验证。1 材料和方法1.1 虫体样本共有三个种群（CoZC、CoAPP和CoAPD）,分别采自北半球加拿大的海狮

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Primary omental torsion:A case report

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World Journal of Gastrointestinal Surgery 2011年第10期3卷 153-155页

作者： stefano Scabini Edoardo Rimini Andrea Massobrio Emanuele Romairone Chiara Linari Renato Scordamaglia Luisito De Marini Valter Ferrando Oncologic Surgical Unit Department of Haemato-OncologySt Martino Hospital Surgical Unit Department of SurgeryUniversity of Firenze

A patient presented with an acute abdomen at the Emergency *** patient,a 69-year-old man,was admitted and underwent surgery with a provisional diagnosis of acute *** surgery,omental torsion was diagnosed and the involved omentum was *** patient had no previous surgical *** torsion is a rare cause of acute abdomen in children and adults who may present with various signs and symptoms;a preoperative diagnosis may therefore be difficult and can usually only be established during surgery.

关键词： Omentum Torsion Abdominal pain

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Memory Nanodevices Based on Carbon Nanotube-Fe-Pt Interconnects： Electromagnetic Simulations and Magnetically Stimulated Nanotube Growth

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材料科学与工程（中英文B版） 2015年第3期5卷 120-134页

作者： stefano Belluccl Federico Micciullal Yuri Shunin Yuri Zhukovskii Viktor Gopejenko Nataly Burlutskaya Tamara Lobanova-Shunina Aldo Capobianchi INFN - Laboratori Nazionali di Frascati Via Enrico Fermi 40 1-00044 Frascati- (Rome) Italy Institute of Solid State Physics University of Latvia L V-1063 Riga Latvia ISMA University LV-1019 Riga Latvia Aviation Institute Riga Technical University L V-1019 Riga Latvia Istituto di Struttura della Materia-CNR A.d.R. Roma Via Salaria Km 29.300 Monterotondo (Rome) Italy

The parametrically controlled production of CNTs (Carbon nanotubes) with predefined morphologies is a topical technological problem for modern nanoelectronics. The CVD (Chemical vapor deposition) technique for SWCNTs (Single walled carbon nanotubes) in the presence of various metal nanoparticle catalysts is generally used now. The application of a magnetically stimulated CVD process scheme and catalyst nanoparticles with a strong magnetism promises additional possibilities for the CVD process management and allows expecting a predictable growth of CNTs with set chiralities and diameters. The main attention is focused on the magnetically anisotropy Pt-Fe in L10 crystallographic phase nanoparticles effect research. The developed theoretical cluster approach based on the multiple scattering and effective medium approximation is used for simulation of fundamental electromagnetic properties in Pt-Fe L10-CNT interconnects, which are responsible for developing CNTs morphologies. The proposed model of "effective bonds" and the model of magnetic stimulation for growing CNTs morphologies generated on the Pt-Fe nanoparticle surface are applied for the evaluation of the expected CNT chiralities distribution. The model and conditions controlled magnetically, which stimulate CNT growth in the CVD process, aimed at the predictable SWCNT diameter and chirality and based on Pt-Fe L10 catalyst are discussed. The possibilities of CNT forest growing on FePt nanoparticles for magnetic nanomemory are also evaluated.

关键词：单壁碳纳米管电磁仿真磁刺激存储器生长互连 Pt纳米粒子纳米催化剂

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蜂螫伤致急性溶血性贫血三例

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中华血液学杂志 2003年第4期24卷 202-202页

作者：吴华新陈劲松冯贵平徐旭四川省绵阳市中心医院 621000

例1，男，20岁。因群蜂鳌伤头部伴局部红肿、疼痛lh于2000年9月20日人院。查体：无贫血貌，皮肤、巩黄染，无出血点、疲斑。头部蜂鳌伤30余处，红肿。双肺无异常，心率56次／min，肝、脾未触及。人院时wBC 24 ．5 ×10L，Hb127g/L，BPC1... 详细信息

例1，男，20岁。因群蜂鳌伤头部伴局部红肿、疼痛lh于2000年9月20日人院。查体：无贫血貌，皮肤、巩黄染，无出血点、疲斑。头部蜂鳌伤30余处，红肿。双肺无异常，心率56次／min，肝、脾未触及。人院时wBC 24 ．5 ×10L，Hb127g/L，BPC147 ×10~9/L,Ret 0．004。'肾mmol/L,肌酐109μmol/L。塞米松30 mg静脉滴注，病情仍进行性加重，患者烦躁、呕吐9h内无尿、黄疽加深，中度贫血貌。心电图示心率44次min。经尿管导出酱油色尿约30ml，尿蛋白（+++（＋＋＋），尿胆原（＋＋）。血红蛋白降至81g/L。肝功ALT 90U／L，AST286U／L，乳酸脱氢酶（LDH）一P 1 527U／L，红素76．0μmol/L，直接胆红素9．2μmol酸脱氢酶（HBDH）870U／L，肌酸磷酸激酶（CK）8 347U／L，肌酸磷酸激酶同功酶（CK-MB）496 U／L。肾功能：尿素17．6 mmL，肌醉181 ．μmol/L。诊断：蜂鳌伤致急性溶血中毒性心肌炎、肝损害、急性肾功能不全。给予血浆置换（PE），在运行至1／2时间患者感头痛、呕吐症状减轻，尿呈茶色，心率升至60次／min。次日因前述症状反复，40次／min，给予第2次PE后病情得到控制。尿色变肤、巩膜黄染减退，心率70～80次／min，继续激素治疗，并渐减量。共住院35d，出院时WBc 4 ．7 ×10~9/L,Hbl0BPC 107×10~9/L，Ret0．020。尿常规，肝、肾功恢复正常。

关键词：蜂螫伤急性溶血性贫血血浆置换疗法病例报告

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Storage lesion:History and perspectives

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World Journal of Hematology 2015年第4期4卷 54-68页

作者： Julien Delobel Olivier Garraud stefano Barelli Jean-Jacques Lefrère Michel Prudent Niels Lion Jean-Daniel Tissot Transfusion Interrégionale CRS Institut National de Transfusion Sanguine Faculty of Medicine University of Lyon Saint-Etienne

Red blood cell concentrates(RBCCs) are the major labile blood component transfused worldwide to rescue severe anemia symptoms. RBCCs are frequently stored in additive solutions at 4 ℃ for up to 42 d, which induces cellular lesion and alters red blood cell metabolism, protein content, and rheological properties. There exists a hot debate surrounding the impact of storage lesion, with some uncertainty regarding how RBCC age may impact transfusion-related adverse clinical outcomes. Several studies show a tendency for poorer outcomes to occur in patients receiving older blood products; however, no clear significant association has yet been demonstrated. Some age-related RBCC alterations prove reversible, while other changes are irreversible following protein oxidation. It is likely that any irreversible damage affects the blood component quality and thus the transfusion efficiency. The present paper aims to promote a better understanding of the occurrence of red blood cell storage lesion, with particular focus on biochemical changes and microvesiculation, through a discussion of the historical advancement of blood transfusion processes.

关键词： Ageing Blood cells Exosomes Microparticles Microvesicles Proteomics Storage Transfusion

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植物节律

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国外科技新书评介 2007年第12期 5-5页

作者： M．stefano 刘毅（博士生）不详中国科学院遗传与发育生物学研究所

植物节律广泛存在于生物界，是生命科学的重大基础理论问题。植物分子生物学技术、显微技术及应用数学等技术的发展，为外界信号及生物钟对节律基因表达调控提供了新的研究手段。本书回顾了近年来植物近昼夜节律、次昼夜节律分子机制相... 详细信息

植物节律广泛存在于生物界，是生命科学的重大基础理论问题。植物分子生物学技术、显微技术及应用数学等技术的发展，为外界信号及生物钟对节律基因表达调控提供了新的研究手段。本书回顾了近年来植物近昼夜节律、次昼夜节律分子机制相关研究所取得的进展，并描述了植物的生长、发育、对周围环境的应激性反应等生理现象。

关键词：昼夜节律植物分子生物学技术基因表达调控基础理论生命科学应用数学显微技术

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Oldest biliary endoprosthesis in situ

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World Journal of Gastrointestinal Endoscopy 2013年第7期5卷 356-358页

作者： Pierluigi Consolo Giuseppe Scalisi stefano F Crinò Andrea Tortora Giuseppa Giacobbe Marcello Cintolo Luigi Familiari Socrate Pallio Digestive Endoscopy Unit University Hospital98100 Messina Italy

The advantages of endoscopic retrograde cholangio-pancreatography over open surgery have made it the predominant method of treating patients with choledo-cholithiasis. After sphincterotomy, however, 10%-15% of common bile duct stones cannot be removed with a basket or balloon. The methods for managing "irretrievable stones" include surgery, mechanical lithotripsy, intraductal or extracorporeal shock wave lithotripsy and biliary stenting. The case presented was a referred 82-year-old Caucasian woman with a 7-year-old plastic biliary endoprosthesis in situ . To the best of our knowledge the examined endoprosthesis is the oldest endo-prosthesis in situ reported in the literature. Endoscopic biliary endoprosthesis placement remains a simple and safe procedure for patients with stones that are difficult to manage by conventional endoscopic methods and for patients who are unfit for surgery or who are high surgical risks. To date no consensus has been reached regarding how long a biliary prosthesis should remain in situ . Long-term biliary stenting may have a role in selected elderly patients if stones extraction has failed because the procedure may prevent stones impaction and cholangitis.

关键词： Common bile duct stones Cholangitis Biliary endoprosthesis Endoscopic retrograde cholangio-pancreatography Endoscopic sphincterotomy

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