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Soil Physico-Chemical Properties and Different Altitudes Affect Arbuscular Mycorrhizal Fungi Abundance and Colonization in Cacao Plantations of Cameroon

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American Journal of Plant Sciences 2024年第2期15卷 57-82页

作者： Franklin Tounkam Ketchiemo Beaulys Fotso Astride stéphanie Mouafi Djabou Victor Jos Eyamo Evina Japhet Youri Essambita Franck Maxime Ewane Tang Nicolas Niemenak Laboratory of Plant Physiology and Biochemistry Department of Biological Sciences Higher Teacher Training College University of Yaounde I Yaounde Cameroon Department of Plant Biology Faculty of Sciences University of Yaounde I Yaounde Cameroon Faculty of Agronomy and Agricultural Sciences Bafia Annex University of Dschang Dschang Cameroon Applied Biotechnology Engineering Laboratory Department of Agriculture and Agropastoral Higher Teacher Training College University of Ebolowa Ebolowa Cameroon Plant Physiology Improvement Unit Laboratory of Biotechnology and Environment Department of Plant Biology Faculty of Sciences University of Yaounde I Yaounde Cameroon

This study aims to investigate the abundance of AMF according to soil properties and altitudes in different cacao plantations of Cameroon. Physico-chemical analyses were made on soil samples collected from three agro-ecological zones. Soil samples were also used to evaluate directly the AMF abundance following the various altitudes and after trapping by sorghum plant. The results showed that soil properties, AMF spore abundances and colonization fluctuated significantly at different altitudes. The most represented texture was sandy loam. The bimodal zone presented a homogeneous texture (sandy loam) in all its localities. Cacao soil chemical characteristics showed that, the highest nitrogen rate (0.47%;p 0.05, Scott-Knott test) was recorded at Melong in a monomodal zone while Tonga in the Western highlands displayed the lowest rate (0.13%). Soil P concentration was significantly high in monomodal zones (Mbanga and Melong). Soil pH level indicated that the soil from Tonga in the Western highlands was neutral (pH = 6.67), and soils of other localities under study were acidic with the lowest (4.75) pH level recorded at Melong in a monomodal zone. In soil samples, the highest spore density (1.03 spores/g soil) was observed at Ntui in Bimodal zone, while the lowest spore density (0.26 spores/g soil) was observed at Bafang in the Western highlands. Root colonization showed that the sample from Bokito in a bimodal zone displayed the best frequency of mycorrhization (86.11%) while the sample from Bafang in the Western highlands recorded the lowest (27.11%). The PCA analysis highlighted that available phosphorus, pH and altitude all strongly correlated with AMF root colonization ability and can be used as a predictor of AMF colonization ability in cacao rhizosphere.

关键词： Agroecological Zone Altitude Variations Arbuscular Mycorrhizal Fungi Soil Properties Theobroma cacao

Individual stages of bacterial dichloromethane degradation mapped by carbon and chlorine stable isotope analysis

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Journal of Environmental Sciences 2019年第4期31卷 147-160页

作者： Maria L.Torgonskaya Anatoly M.Zyakun Yuri A.Trotsenko Kestutis S.Laurinavichius steffen Kümmel stéphane Vuilleumier Hans H.Richnow Laboratory of Radioactive Isotopes G.K.Skryabin Institute of Biochemistry and Physiology of MicroorganismsFRC Pushchino Center for Biological ResearchRussian Academy of Sciences Laboratory of Mass Spectrometry G.K.Skryabin Institute of Biochemistry and Physiology of MicroorganismsFRC Pushchino Center for Biological ResearchRussian Academy of Sciences Department of Isotope Biogeochemistry Helmholtz Centre for Environmental Research-UFZ Université de strasbourg CNRS

The fractionation of carbon and chlorine stable isotopes of dichloromethane(CH_2Cl_2,DCM)upon dechlorination by cells of the aerobic methylotroph Methylobacterium extorquens DM4 and by purified DCM dehalogenases of the glutathione S-transferase family was *** effects for individual steps of the multi-stage DCM degradation process,including transfer across the cell wall from the aqueous medium to the cell cytoplasm,dehalogenase binding,and catalytic reaction,were *** observed carbon and chlorine isotope fractionation accompanying DCM consumption by cell supensions and enzymes was mainly determined by the breaking of C\Cl bonds,and not by inflow of DCM into *** isotope effects of DCM dehalogenation were initially masked in high density cultures,presumably due to inverse isotope effects of non-specific DCM oxidation under conditions of oxygen *** cofactor supply remarkably affected the correlation of variations of DCM carbon and chlorine stable isotopes(Δδ^(13)C/Δδ^(37)Cl),increasing corresponding ratio from 7.2–8.6 to 9.6–10.5 under conditions of glutathione *** suggests that enzymatic reaction of DCM with glutathione thiolate may involve stepwise breaking and making of bonds with the carbon atom of DCM,unlike the uncatalyzed reaction,which is a one-stage process,as shown by quantum-chemical modeling.

关键词： Dichloromethane(DCM) Microbial degradation Aerobic methylotrophic bacteria Dichloromethane dehalogenase Carbon and chlorine isotopic fractionation

Molecular phylogeny of Trametes and related genera,and description of a new genus Leiotrametes

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Fungal Diversity 2012年第4期55卷 47-64页

作者： stéphane Welti Pierre-Arthur Moreau Anne Favel Régis Courtecuisse Mireille Haon David Navarro Sabine Taussac Laurence Lesage-Meessen INRA Aix-Marseille Univ.UMR1163 de Biotechnologie des Champignons Filamenteux ESIL163 avenue de LuminyCP 92513288 MarseilleCedex 09France Facultédes sciences pharmaceutiques et biologiques Laboratoire des sciences végétales et fongiquesUniversitéLille 2.3 rue du Professeur LaguesseB.P.83F-59006 LilleCedexFrance A.Favel:M.Haon:D.Navarro:S.Taussac:L.Lesage-Meessen CIRM-CF UMR 1163 BCF13009 MarseilleFrance

A phylogenetic analysis of Trametes and related taxa is proposed,based on a wide sampling of temperate and tropical *** sequences of ribosomal DNA(ITS1-5.8S-ITS2)and RPB2 gene from mycelia cultures were analyzed by Maximum Likelihood and Bayesian methods,whilst morphological features were documented from the corresponding herbarium *** results were obtained from analyses of ribosomal LSU sequences downloaded from *** Bayesian analysis of ITS+RPB2 sequences gave the best resolution for the phylogenetic reconstructions and revealed the existence of three main lineages in the Trametes-clade:1)a monospecific lineage represented by Artolenzites elegans 2)a lineage including the genus Pycnoporus in its traditional sense and several species usually classified in the genus Trametes(***,***,***,***)&3)a lineage corresponding to the core genus Trametes,including type species of Trametes,Coriolopsis and *** presence of a pseudostipe,aspect and structure of the abhymenial surface,colour change with 5%aqueous solutions of potassium hydroxide and topography of pigments on skeletal hyphae gave relevant morphological support to these *** the structure of the hymenial surface,presence of a black line below the tomentum and color of context(except for genus Pycnoporus)usually used in traditional polypore-classifications did not reveal any phylogenetic significance.A partial systematic arrangement of the Trametes clade is proposed,with the introduction of a new genus:Leiotrametes Welti&***..Two new combinations:Leiotrametes lactinea(Berk.)Welti&*** ***(Berk.)Welti&*** proposed.

关键词： Basidiomycota Polyporales Artolenzites Coriolopsis Lenzites Pycnoporus Morphology Biogeography

Sirolimus-eluting stent fractures associated with aneurysm and very late stent thrombosis in the right coronary artery

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上海医学 2007年第Z1期30卷 226-229页

作者： LIU Xuebo Gary S.Mintz stéphane G.Carlier Martin B.Leon Department of Cardiology Zhongshan HospitalFudan University Columbia University Medical Center and Cardiovassular Research Faundation New York

Although the occurrence of coronary stent fracture is rare, recent reports showed that stent fracture after sirolimus-eluting stent (SES) implantation may be associated with neointimal hyperplasia and restenosis. We report two cases of stent fracture that occurred late after elective SES implantation into the right coronary artery (RCA) that were related to the aneurysm, restenosis, thrombosis, and vessel occlusion.

关键词： stent aneurysm eluting restenosis elective implantation distal occlusion longitudinal unstable

A machine vision tool for facilitating the optimization of large-area perovskite photovoltaics

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npj Computational Materials 2021年第1期7卷 1764-1773页

作者： Nina Taherimakhsousi Mathilde Fievez Benjamin P.MacLeod Edward P.Booker Emmanuelle Fayard Muriel Matheron Matthieu Manceau stéphane Cros Solenn Berson Curtis P.Berlinguette Department of Chemistry The University of British Columbia2036 Main MallVancouverBC V6T 1Z1Canada Univ.Grenoble Alpes CEALITENCampus Ines73375 Le Bourget du LacFrance stewart Blusson Quantum Matter Institute The University of British Columbia2355 East MallVancouverBC V6T 1Z4Canada School of Electrical Engineering and Computer Science University of Ottawa800 King Edward AvenueOttawaON K1N 6N5USA Department of Chemical&Biological Engineering The University of British Columbia2360 East MallVancouverBC V6T 1Z3Canada Canadian Institute for Advanced Research(CIFAR) MaRS Innovation Centre661 University Avenue Suite 505M5G 1M1 TorontoONCanada

We report a fast,reliable and non-destructive method for quantifying the homogeneity of perovskite thin films over large areas using machine *** adapt existing machine vision algorithms to spatially quantify multiple perovskite film properties(substrate coverage,film thickness,defect density)with pixel resolution from pictures of 25 cm2 *** machine vision tool—called PerovskiteVision—can be combined with an optical model to predict photovoltaic cell and module current density from the perovskite film *** use the measured film properties and predicted device current density to identify a posteriori the process conditions that simultaneously maximize the device performance and the manufacturing throughput for large-area perovskite deposition using gas-knife assisted slot-die *** thus facilitates the transfer of a new deposition process to large-scale photovoltaic module *** work shows how machine vision can accelerate slow characterization steps essential for the multi-objective optimization of thin film deposition processes.

关键词： optimization perovskite film

Proteomic and metabolomic profiling underlines the stage- and time-dependent effects of high temperature on grape berry metabolism

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Journal of Integrative Plant Biology 2020年第8期62卷 1132-1158页

作者： David Lecourieux Christian Kappel stéphane Claverol Philippe Pieri Regina Feil John E.Lunn Marc Bonneu Lijun Wang Eric Gomès Serge Delrot Fatma Lecourieux UMR1287 EGFV INRAEBordeaux Sciences AgroBordeaux UniversityISVV33140 Villenave d'OrnonFrance Institut of Biochemistry and Biology Potsdam UniversityD-14476 PotsdamGermany Proteome Platform Bordeaux Functional Genomic CenterBordeaux University33076 BordeauxFrance Max Planck Institute of Molecular Plant Physiology 14476 PotsdamGolmGermany Institute of Botany the Chinese Academy of SciencesBeijing 100093China UMR1287 EGFV CNRSINRAEBordeaux Sciences AgroBordeaux UniversityISVV33140 Villenave d'OrnonFrance

Climate change scenarios predict an increase in mean air temperatures and in the frequency,intensity,and length of extreme temperature events in many wine-growing regions *** elevated temperature has detrimental effects on berry growth and composition,it threatens the economic and environmental sustainability of wine *** Cabernet Sauvignon fruit-bearing cuttings,we investigated the effects of high temperature(HT)on grapevine berries through a label-free shotgun proteomic analysis coupled to a complementary metabolomic *** the 2,279 proteins identified,592 differentially abundant proteins were found in berries exposed to *** gene ontology categories“stress,”“protein,”“secondary metabolism,”and“cell wall”were predominantly altered under *** temperatures strongly impaired carbohydrate and energy metabolism,and the effects depended on the stage of development and duration of *** amounts correlated poorly with protein expression levels in HT berries,highlighting the value of proteomic studies in the context of heat ***,this work reveals that HT alters key proteins driving berry development and ***,we provide a list of differentially abundant proteins that can be considered as potential markers for developing or selecting grape varieties that are better adapted to warmer climates or extreme heat waves.

关键词： metabolism extreme high

A Kinematics Scalar Projection Method (KSP) for Incompressible Flows with Variable Density

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Open Journal of Fluid Dynamics 2015年第2期5卷 171-182页

作者： Jean-Paul Caltagirone stéphane Vincent UMR CNRS 5295 Département TREFLE Institut de Mécanique et d’Ingénierie Université de Bordeaux Pessac Cedex France UMR CNRS 8208 Laboratoire Modélisation et Simulation Multi Echelle (MSME) Université Paris-Est Marne-La-Vallée France

A new scalar projection method presented for simulating incompressible flows with variable density is proposed. It reverses conventional projection algorithm by computing first the irrotational component of the velocity and then the pressure. The first phase of the projection is purely kinematics. The predicted velocity field is subjected to a discrete Hodge-Helmholtz decomposition. The second phase of upgrade of pressure from the density uses stokes’ theorem to explicitly compute the pressure. If all or part of the boundary conditions is then fixed on the divergence free physical field, the system required to be solved for the scalar potential of velocity becomes a Poisson equation with constant coefficients fitted with Dirichlet conditions.

关键词： Projection Methods Hodge-Helmholtz Decomposition Navier-stokes Equation Incompressible Flows

边缘下皮质(IL)的动态改变以及IL对前边缘皮质(PL)的调控参与大鼠慢性炎症痛

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中国疼痛医学杂志 2024年第7期30卷 158-158页

作者：刘风雨(译) 北京大学神经科学研究所

慢性疼痛是临床的常见病。内侧前额叶皮质(medial prefrontal cortex,mPFC)参与慢性疼痛发病机制。前边缘皮质(prelimbic cortex,PL)是mPFC的一个核心亚区,介导慢性疼痛。边缘下皮质(IL)是mPFC的另一个亚区,也能够调控伤害性反应。神经... 详细信息

慢性疼痛是临床的常见病。内侧前额叶皮质(medial prefrontal cortex,mPFC)参与慢性疼痛发病机制。前边缘皮质(prelimbic cortex,PL)是mPFC的一个核心亚区,介导慢性疼痛。边缘下皮质(IL)是mPFC的另一个亚区,也能够调控伤害性反应。神经解剖学研究表明,IL深层的兴奋性锥体神经元通过支配PL深层的抑制性中间神经元,进而作用到PL局部的锥体神经元上,调控PL的功能。IL、PL以及IL-PL环路如何参与慢性疼痛的发病机制,不甚清晰。研究者采用完全弗氏佐剂(complete Freund's adjuvant,CFA)建立的大鼠慢性炎症痛模型,结合体电生理记录和化学遗传学等方法,深入解析IL、PL及其环路在慢性疼痛中的作用。

关键词：中间神经元完全弗氏佐剂慢性疼痛神经解剖学锥体神经元大鼠发病机制

Predictive factors of survival in patients treated with definitive chemoradiotherapy for squamous cell esophageal carcinoma

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World Journal of Gastroenterology 2006年第26期12卷 4185-4190页

作者： Frédéric Di Fiore stéphane Lecleire Olivier Rigal Marie-Pierre Galais Emmanuel Ben Soussan Isabelle David Bernard Paillot Jacques-Henri Jacob Pierre Michel Digestive Oncology Unit Hepatogastroenterology Department Rouen University HospitalFrance Digestive Oncology unit Francois Baclesse Centre CaenFrance Radiotherapy Department Henri Becquerel Centre RouenFrance

AIM： The aim of the study was to evaluate the predictive factors of survival in patients with locally advanced squamous cell esophageal carcinoma （LASCOC） treated with definitive chemoradiotherapy （CRT） regimen based on the 5FU/CDDP combination. METHODS： All patients with LASCOC treated with a definitive CRT using the 5FU/CDDP combination between 1994 and 2000 were retrospectively included. Clinical complete response （CCR） to CRT was assessed by esophageal endoscopy and C-F-scan 2 mo after CRT completion. Prognostic factors of survival were assessed using univariate and multivariate analysis by the Cox regression model. RESULTS： A total of 116 patients were included in the study. A CCR to CRT was observed in 86/116 （74.1%）. The median survival was 20 mo （range 2-114） and the 5-year survival was 9.4%. Median survival of responder patients to CRT was 25 mo （range 3-114） as compared to 9 mo （range 2-81） in non-responder patients （P 〈 0.001）. In univariate analysis, survival was associated with CCR （P 〈 0.001）, WHO performance status 〈 2 （P = 0.01）, tumour length 〈 6 cm （P = 0.045） and weight loss 〈 10% was in limit of significance （P = 0.053）. In multivariate analysis, survival was dependant to CCR （P 〈 0.0001）, weight loss 〈 10% （P = 0.034） and WHO performance 〈 2 （P = 0.046）. CONCLUSION： Our results suggest that survival in patients with LASCOC b＇eated with definitive CRT was correlated to CCR, weight loss and WHO performance status.

关键词： Definitive chemoradiotherapy Esophageal squamous cell carcinoma Predictive factors